Detailed view for Tb927.10.16070

Basic information

TDR Targets ID: 19374
Trypanosoma brucei, Eukaryotic translation initiation factor 4E-2
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.6459 | Length (AA): 251 | MW (Da): 28299 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01652   Eukaryotic initiation factor 4E

Gene Ontology

Mouse over links to read term descriptions.
GO:0005737   cytoplasm  
GO:0003743   translation initiation factor activity  
GO:0003723   RNA binding  
GO:0006413   translational initiation  

Metabolic Pathways

RNA transport (KEGG)

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Bloodstream Form. Siegel TN
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129419)

Species Accession Gene Product
Arabidopsis thaliana AT5G18110   translation initiation factor NCBP
Brugia malayi Bm1_32915   Eukaryotic translation initiation factor 4E type 3, putative
Caenorhabditis elegans CELE_C05D9.5   Protein IFE-4
Dictyostelium discoideum DDB_G0284841   hypothetical protein
Dictyostelium discoideum DDB_G0291820   hypothetical protein
Drosophila melanogaster Dmel_CG33100   eIF4E-Homologous Protein
Giardia lamblia GL50803_7990   Translation elongation factor
Homo sapiens ENSG00000135930   eukaryotic translation initiation factor 4E family member 2
Loa Loa (eye worm) LOAG_04336   eukaryotic translation initiation factor 4E type 3
Mus musculus ENSMUSG00000026254   eukaryotic translation initiation factor 4E member 2
Neospora caninum NCLIV_057910   hypothetical protein
Oryza sativa 4332327   Os03g0262400
Schmidtea mediterranea mk4.000548.03   4EHP
Trypanosoma brucei gambiense Tbg972.10.19680   eukaryotic translation initiation factor 4e, putative
Trypanosoma brucei Tb927.10.16070   Eukaryotic translation initiation factor 4E-2
Trypanosoma congolense TcIL3000_0_03820   Eukaryotic translation initiation factor 4E-2
Toxoplasma gondii TGME49_315150   eukaryotic initiation factor-4E, putative
Trichomonas vaginalis TVAG_450940   eukaryotic translation initiation factor 4E, putative

Essentiality

Tb927.10.16070 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.16070 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.16070 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.16070 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.16070 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C05D9.5 Caenorhabditis elegans embryonic lethal wormbase
TGME49_315150 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Tb927.10.16070 (Trypanosoma brucei), Eukaryotic translation initiation factor 4E-2
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