Detailed view for Tb927.4.2560

Basic information

TDR Targets ID: 20244
Trypanosoma brucei, cardiolipin synthetase
Source Database / ID:  TriTrypDB  GeneDB

pI: 10.1837 | Length (AA): 639 | MW (Da): 73791 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00614   Phospholipase D Active site motif
PF13091   PLD-like domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_129472)

Species Accession Gene Product
Babesia bovis BBOV_II002000   phospholipase D active site motif family protein
Cryptosporidium hominis Chro.30333   cardiolipin synthetase
Cryptosporidium parvum cgd3_2940   probable phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthase, 2x SMART_PLDc domains, possible bacterial origin
Chlamydia trachomatis CT_284   phospholipase D superfamily protein
Dictyostelium discoideum DDB_G0284687   phospholipase D
Escherichia coli b0789   cardiolipin synthase 2
Escherichia coli b1249   cardiolipin synthase 1
Leishmania braziliensis LbrM.20.1600   cardiolipin synthetase, putative
Leishmania donovani LdBPK_341860.1   cardiolipin synthetase, putative
Leishmania infantum LinJ.34.1860   cardiolipin synthetase, putative
Leishmania major LmjF.34.2110   cardiolipin synthetase, putative
Leishmania mexicana LmxM.33.2110   cardiolipin synthetase, putative
Neospora caninum NCLIV_068780   hypothetical protein
Neospora caninum NCLIV_054060   hypothetical protein
Plasmodium berghei PBANKA_0108000   mitochondrial cardiolipin synthase, putative
Plasmodium falciparum PF3D7_0609400   mitochondrial cardiolipin synthase, putative
Plasmodium knowlesi PKNH_1140800   mitochondrial cardiolipin synthase, putative
Plasmodium vivax PVX_113625   cardiolipin synthetase, putative
Plasmodium yoelii PY06901   cardiolipin synthetase
Trypanosoma brucei gambiense Tbg972.4.2520   cardiolipin synthetase, putative
Trypanosoma brucei Tb927.4.2560   cardiolipin synthetase
Trypanosoma brucei Tb11.v5.0672   cardiolipin synthetase, putative
Trypanosoma congolense TcIL3000_4_2440   cardiolipin synthetase, putative
Trypanosoma cruzi TcCLB.506631.20   cardiolipin synthetase, putative
Trypanosoma cruzi TcCLB.506559.40   cardiolipin synthetase, putative
Toxoplasma gondii TGME49_309940   phospholipase D active site domain-containing protein
Theileria parva TP04_0329   cardiolipin synthetase, putative

Essentiality

Tb927.4.2560 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.2560 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.2560 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.2560 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.2560 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0789 Escherichia coli non-essential goodall
b1249 Escherichia coli non-essential goodall
TGME49_309940 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.1264 0.3402 1
0.1049 0.3449 1
0.1642 0.346 1
0.0379 0.3338 1
0.1495 0.3456 1
0.0544 0.3311 1
0.1556 0.3192 1
0.1957 0.3442 1
0.1323 0.3431 1
0.157 0.3471 1
0.0304 0.2983 1
0.1347 0.3464 1
0.1708 0.3446 1
0.0613 0.3192 1
0.0127 0.2605 1
0.0648 0.3345 1
0.02 0.3304 1
0.1494 0.3265 1
0.0301 0.3317 1
0.0478 0.3327 1
0.03 0.3241 0.8159
0.0311 0.324 1
0.0442 0.3306 1
0.0143 0.3192 1
0.0043 0.2613 1
0.1323 0.3431 1
0.1495 0.3456 1
0.087 0.3372 1
0.1239 0.3458 1
0.0556 0.3452 1
0.0432 0.3414 1
0.0868 0.3379 1
0.0383 0.3057 1
0.1951 0.3411 1
0.1175 0.3472 1
0.0285 0.3015 1
0.1136 0.3456 1
0.084 0.3382 1
0.1971 0.347 1
0.0035 0.2579 1
0.0067 0.3045 1
0.0728 0.307 1
0.0859 0.3451 1
0.0304 0.3361 1
0.0314 0.336 1
0.0868 0.3474 1
0.0263 0.3394 1
0.0123 0.2575 1
0.1049 0.3449 1
0.0272 0.2864 0.7643
0.0319 0.3257 1
0.1602 0.3425 1
0.1276 0.3238 1
0.0892 0.3453 1
0.0442 0.3306 1
0.1012 0.3389 1
0.0034 0.3048 1
0.1983 0.3441 1
0.1964 0.3423 1
0.0097 0.3101 1
0.1323 0.3431 1
0.0929 0.3428 1
0.1918 0.344 1
0.2209 0.3473 1
0.0504 0.2658 1
0.1049 0.3449 1
0.0294 0.3076 1
0.1959 0.3424 1
0.0254 0.3265 1
0.0814 0.3379 1
0.1623 0.344 1
0.0201 0.3268 1
0.1026 0.3351 1
0.1863 0.3478 1
0.0579 0.3385 0.5466
0.181 0.3465 1
0.0808 0.3466 1
0.1323 0.3431 1
0.0431 0.3311 1
0.1939 0.3424 1
0.0264 0.3095 1
0.0432 0.3414 1
0.0968 0.3389 1
0.1492 0.3399 1
0.0332 0.3014 1
0.0043 0.2504 1
0.016 0.2955 1
0.1352 0.3451 1
0.0449 0.3367 1
0.1276 0.3238 1
0.0268 0.3274 1
0.124 0.3407 1
0.1134 0.347 1
0.014 0.2945 1
0.1642 0.346 1
0.2131 0.3441 1
0.03 0.3361 1
0.00582448 0.272382 1
0.1694 0.3462 1
0.0339 0.3192 1
0.0315 0.3075 1
0.1844 0.3443 1
0.1667 0.3465 1
0.0847 0.3401 1
0.1885 0.3442 1
0.0561 0.3059 1
0.1506 0.3435 1
0.1738 0.3477 1
0.175 0.3462 1
0.0698 0.3286 1
0.1881 0.3192 1
0.1444 0.3443 1
0.1971 0.347 1
0.1041 0.3435 1
0.0399 0.3359 1
0.0316 0.3307 1
0.0733 0.3424 1
0.0336 0.326 1
0.0411 0.3382 1
0.1875 0.3459 1
0.0332 0.3014 1
0.1583 0.3436 1
0.1718 0.3463 1
0.0525 0.3328 1
0.0354 0.2706 1
0.1453 0.3435 1
0.0354 0.2706 1
0.2048 0.3447 1
0.0437 0.3294 1
0.099 0.3452 1
0.1667 0.3465 1
0.1825 0.3433 1
0.083 0.336 1
0.0805 0.343 1
0.0865 0.3192 1
0.181 0.344 1
0.031 0.3082 1
0.0345 0.3056 1
0.0398 0.3055 1
0.0042 0.2573 1
0.1934 0.3064 1
0.0781 0.3319 1
0.0929 0.3428 1
0.1667 0.3465 1
0.157 0.3471 1
0.0808 0.3424 1
0.1012 0.3389 1
0.1483 0.3402 1
0.0892 0.3192 1
0.1336 0.3469 1
0.1339 0.3451 1
0.0344 0.3343 1
0.1997 0.3445 1
0.1001 0.336 1
0.1467 0.3455 1
0.037 0.3258 1
0.1517 0.3472 1
0.0845 0.3375 1
0.0369 0.332 1
0.203 0.3464 1
0.2209 0.3473 1
0.1842 0.2724 1
0.1568 0.3437 1
0.2035 0.3434 1
0.2209 0.3473 1
0.0802 0.3369 1
0.099 0.3452 1
0.068299 0.308661 1
0.1539 0.3459 1
0.1302 0.3454 1
0.2035 0.3434 1
0.0058 0.2869 1
0.0458 0.3358 1
0.0181 0.2774 1
0.0063 0.2572 1
0.126 0.3396 1
0.0036 0.2854 1
0.1528 0.3477 1
0.0197 0.33 1
0.1939 0.3423 1
0.0781 0.3319 1
0.1667 0.3465 1
0.1863 0.3478 1
0.1652 0.3192 1
0.1524 0.3426 1
0.1215 0.3373 1
0.1722 0.343 1
0.2352 0.3472 1
0.1526 0.3449 1
0.0036 0.2854 1
0.126 0.3396 1
0.0201 0.3064 1
0.1504 0.3431 1
0.1448 0.3417 1
0.1149 0.2888 1
0.0897 0.3192 1
0.0317 0.3053 1
0.1971 0.347 1
0.1468 0.3452 1
0.2149 0.3453 1
0.1768 0.3442 1
0.2174 0.345 1
0.0352 0.3362 1
0.1164 0.3403 1
0.0242 0.3192 1
0.1236 0.3431 1
0.1323 0.3431 1
0.0035 0.2582 1
0.1863 0.3421 1
0.1743 0.3441 1
0.0321 0.3356 1
0.0314 0.3362 1
0.1539 0.3459 1
0.1136 0.3447 1
0.0907 0.336 1
0.0432 0.3414 1
0.0393 0.2504 1
0.0245 0.3136 1
0.1158 0.3469 1
0.2007 0.3035 1
0.166 0.344 1
0.0398 0.3402 1
0.0504 0.2658 1
0.0259 0.2975 1
0.1622 0.3469 1
0.039 0.3398 1
0.2106 0.3445 1
0.0131 0.3067 1
0.0251 0.2713 1
0.0327 0.3322 1
0.0501 0.3327 1
0.0159 0.2992 1
0.224 0.3436 1
0.2146 0.3451 1
0.1359 0.3475 1
0.0314 0.3362 1
0.0042 0.2833 1
0.2317 0.2554 1
0.2352 0.3472 1
0.0711 0.3359 1
0.0308 0.327 1
0.039 0.3398 1
0.1379 0.346 1
0.0972 0.3381 1
0.0042 0.2951 1
0.1921 0.341 1
0.0406 0.3349 1
0.203 0.3464 1
0.0321 0.3438 1
0.0317 0.3053 1
0.0035 0.2598 1
0.1374 0.3057 1
0.1523 0.3432 1
0.173 0.3442 1
0.0344 0.3363 1
0.0808 0.3424 1
0.0405 0.3379 1
0.071 0.3451 1
0.0432 0.3414 1
0.1716 0.3454 1
0.0892 0.3453 1
0.2209 0.3473 1
0.0143 0.2792 1
0.0831 0.3391 1
0.1346 0.3186 1
0.0264 0.3198 1
0.1854 0.3461 1
0.1971 0.347 1
0.0583 0.3253 1
0.0375 0.2715 1
0.0311 0.324 1
0.0512 0.3343 1
0.032 0.3402 1
0.1971 0.3443 1
0.0366 0.3362 1
0.2352 0.3472 1
0.0788 0.3065 1
0.0338 0.3428 0.8148
0.0815 0.3192 1
0.0312 0.2976 1
0.1502 0.3474 1
0.0345 0.3056 1
0.0211 0.3362 1
0.0667 0.3132 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

2 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tb927.4.2560 (Trypanosoma brucei), cardiolipin synthetase
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