Detailed view for PF3D7_1312000

Basic information

TDR Targets ID: 2059
Plasmodium falciparum, malonyl CoA-acyl carrier protein transacylase
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 8.7458 | Length (AA): 403 | MW (Da): 46305 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00698   Acyl transferase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0020011   apicoplast  
GO:0016740   transferase activity  
GO:0004314   [acyl-carrier-protein] S-malonyltransferase activity  
GO:0008152   metabolic process  
GO:0006633   fatty acid biosynthetic process  

Metabolic Pathways

Fatty acid biosynthesis (KEGG)
Global map (KEGG)
Fatty acid metabolism (KEGG)

Structural information

Modbase 3D models:

There are 11 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
19 403 2hg4 (A) 433 846 16.00 0 1 0.99 -0.32
99 403 2c2n (A) 24 336 30.00 0 1 1.19 -1.81
101 393 1mla () 3 289 31.00 0 1 1.28 -1.78
3 395 4mz0 (A) 443 875 16.00 0 1 1.11929 0.62
96 397 3tqe (A) 0 294 28.00 0 1 1.20848 -1.13
100 392 3h0p (A) 2 288 31.00 0 1 1.20295 -0.99
2 399 2qo3 (A) 450 848 15.00 0 1 1.13549 0.58
96 397 3tqe (A) 0 294 28.00 0 1 1.20928 -1.14
100 399 3h0p (A) 2 295 31.00 0.000000051 1 1.21302 -0.96
101 395 3ezo (A) 1 288 28.00 0 1 1.18771 -1.09
156 365 4amm (A) 618 812 32.00 0.00000023 0.99 0.756792 0.4

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Oocyst. Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile gametocyte, Female Gametocyte. PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile intra-erythrocytic - 0 hs, Ring, Male Gametocyte. Otto TD Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, Sporozoite. Otto TD Zanghi G
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_127857)

Species Accession Gene Product
Arabidopsis thaliana AT2G30200   [acyl-carrier-protein] S-malonyltransferase
Candida albicans CaO19.6643   similar to bacterial/mitochondrial malonyl CoA:acyl carrier transacylase
Candida albicans CaO19.13964   similar to bacterial/mitochondrial malonyl CoA:acyl carrier transacylase
Caenorhabditis elegans CELE_C50D2.9   Protein C50D2.9
Chlamydia trachomatis CT_238   malonyl CoA-ACP transacylase
Dictyostelium discoideum DDB_G0270558   hypothetical protein
Drosophila melanogaster Dmel_CG7842   bad egg
Escherichia coli b1092   malonyl-CoA-[acyl-carrier-protein] transacylase
Echinococcus granulosus EgrG_000839400   Malonyl coenzyme A acyl carrier protein transacylase
Echinococcus multilocularis EmuJ_000839400   Malonyl coenzyme A acyl carrier protein transacylase
Homo sapiens ENSG00000100294   malonyl CoA:ACP acyltransferase (mitochondrial)
Mycobacterium leprae ML1653   MALONYL CoA-ACYL CARRIER PROTEIN TRANSACYLASE FABD (Malonyl CoA:AcpM acyltransferase) (MCT)
Mus musculus ENSMUSG00000048755   malonyl CoA:ACP acyltransferase (mitochondrial)
Mycobacterium tuberculosis Rv2243   Malonyl CoA-acyl carrier protein transacylase FabD (malonyl CoA:ACPM acyltransferase) (MCT)
Mycobacterium ulcerans MUL_1306   malonyl CoA-acyl carrier protein transacylase FabD
Neospora caninum NCLIV_046840   Acyl-carrier-protein S-malonyltransferase (EC 2.3.1.39), related
Oryza sativa 4332547   Os03g0297400
Plasmodium berghei PBANKA_1410500   malonyl CoA-acyl carrier protein transacylase, putative
Plasmodium falciparum PF3D7_1312000   malonyl CoA-acyl carrier protein transacylase
Plasmodium knowlesi PKNH_1412700   malonyl CoA-acyl carrier protein transacylase, putative
Plasmodium vivax PVX_122435   malonyl CoA-acyl carrier protein transacylase precursor, putative
Plasmodium yoelii PY05492   malonyl CoA-acyl carrier protein transacylase precursor
Schistosoma japonicum Sjp_0302820   ko:K00645 [acyl-carrier-protein] S-malonyltransferase [EC2.3.1.39], putative
Schistosoma mansoni Smp_072090.2   [ACP] S-malonyltransferase
Schistosoma mansoni Smp_072090.3   [ACP] S-malonyltransferase
Schistosoma mansoni Smp_072090.4   [ACP] S-malonyltransferase
Schistosoma mansoni Smp_072090.1   [ACP] S-malonyltransferase
Schmidtea mediterranea mk4.013551.00   Malonyl-CoA-acyl carrier protein transacylase, mitochondrial
Schmidtea mediterranea mk4.052433.00  
Schmidtea mediterranea mk4.005521.05   Malonyl-CoA-acyl carrier protein transacylase, mitochondrial
Schmidtea mediterranea mk4.004448.02   Malonyl-CoA-acyl carrier protein transacylase, mitochondrial
Schmidtea mediterranea mk4.010055.00   Malonyl-CoA-acyl carrier protein transacylase, mitochondrial
Toxoplasma gondii TGME49_225990   acyl transferase domain-containing protein
Wolbachia endosymbiont of Brugia malayi Wbm0402   ACP S-malonyltransferase

Essentiality

PF3D7_1312000 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b1092 Escherichia coli essential goodall
PBANKA_1410500 Plasmodium berghei Dispensable plasmo
TGME49_225990 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Trypanosoma cruzi cAMP-dependent protein kinase catalytic subunit 3 337 aa 23.0% 274 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Reagent:
  • Target Type Source Notes
    PF3D7_1312000 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Plasmodium falciparum ( 1 )

Bibliographic References

2 literature references were collected for this gene.

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Gene identifier PF3D7_1312000 (Plasmodium falciparum), malonyl CoA-acyl carrier protein transacylase
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