Detailed view for Tb927.10.12500

Basic information

TDR Targets ID: 20698
Trypanosoma brucei, P-type H+-ATPase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.2168 | Length (AA): 912 | MW (Da): 100228 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 7

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00122   E1-E2 ATPase
PF00690   Cation transporter/ATPase, N-terminus
PF00702   haloacid dehalogenase-like hydrolase

Gene Ontology

Mouse over links to read term descriptions.
GO:0008553   hydrogen-exporting ATPase activity, phosphorylative mechanism  
GO:0120029   GO:proton export across plasma membrane  

GO:0016021   integral to membrane  
GO:0016020   membrane  
GO:0005524   ATP binding  
GO:0003824   catalytic activity  
GO:0000166   nucleotide binding  
GO:0016887   ATPase activity  
GO:0008152   metabolic process  
GO:0006812   cation transport  
GO:0006754   ATP biosynthetic process  

Metabolic Pathways

Oxidative phosphorylation (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
37 882 5ksd (A) 15 844 32.00 0.00000019 1 1.23803 0.32
53 876 5ksd (A) 31 838 38.00 0 1 1.26611 0.11
349 499 1q3i (A) 379 591 17.00 0 0.54 0.13737 -0.24
495 632 1nnl (A) 86 216 21.00 0 0.5 0.234116 0.41

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127253)

Species Accession Gene Product
Arabidopsis thaliana AT4G11730   putative plasma membrane-type ATPase
Arabidopsis thaliana AT2G18960   H(+)-ATPase 1
Arabidopsis thaliana AT2G24520   H(+)-ATPase 5
Arabidopsis thaliana AT3G47950   H(+)-ATPase 4
Arabidopsis thaliana AT5G57350   H(+)-ATPase 3
Arabidopsis thaliana AT1G17260   autoinhibited H(+)-ATPase isoform 10
Arabidopsis thaliana AT5G62670   H(+)-ATPase 11
Arabidopsis thaliana AT3G60330   H(+)-ATPase 7
Arabidopsis thaliana AT3G42640   H(+)-ATPase 8
Arabidopsis thaliana AT2G07560   H(+)-ATPase 6
Arabidopsis thaliana AT4G30190   H(+)-ATPase 2
Arabidopsis thaliana AT1G80660   H(+)-ATPase 9
Candida albicans CaO19.12838   adenosine triphosphatase
Candida albicans CaO19.5383   adenosine triphosphatase
Dictyostelium discoideum DDB_G0268810   magnesium-translocating P-type ATPase
Dictyostelium discoideum DDB_G0282817   P-type ATPase
Escherichia coli b4242   magnesium transporter
Leishmania braziliensis LbrM.18.1740   P-type H -ATPase, putative
Leishmania braziliensis LbrM.18.1550   P-type H -ATPase, putative
Leishmania donovani LdBPK_181510.1   P-type H+-ATPase 1B, putative
Leishmania infantum LinJ.18.1490   P-type H -ATPase, putative
Leishmania infantum LinJ.18.1500   P-type H -ATPase, putative
Leishmania infantum LinJ.18.1510   P-type H -ATPase, putative
Leishmania major LmjF.18.1510   P-type H -ATPase, putative
Leishmania major LmjF.18.1520   P-type H -ATPase, putative
Leishmania mexicana LmxM.18.1510   P-type H -ATPase, putative
Leishmania mexicana LmxM.18.1520   P-type H -ATPase, putative
Neospora caninum NCLIV_022240   ATPase, related
Oryza sativa 4331853   Os03g0183900
Oryza sativa 4337257   Os04g0656100
Oryza sativa 4342621   Os07g0191200
Oryza sativa 4331015   Os02g0797300
Oryza sativa 4331281   Os03g0100800
Oryza sativa 4333770   Os03g0689300
Oryza sativa 4340312   Os06g0181500
Oryza sativa 4352910   Os12g0638700
Oryza sativa 4338405   Os05g0319800
Saccharomyces cerevisiae YPL036W   H(+)-exporting P2-type ATPase PMA2
Saccharomyces cerevisiae YGL008C   H(+)-exporting P2-type ATPase PMA1
Trypanosoma brucei gambiense Tbg972.10.15070   P-type H -ATPase, putative
Trypanosoma brucei Tb927.10.12510   P-type H+-ATPase, putative
Trypanosoma brucei Tb927.10.12500   P-type H+-ATPase, putative
Trypanosoma congolense TcIL3000_10_10700   P-type H+-ATPase, putative
Trypanosoma cruzi TcCLB.506333.10   plasma-membrane proton-efflux P-type ATPase, putative
Trypanosoma cruzi TcCLB.510575.5   proton motive ATPase, putative
Trypanosoma cruzi TcCLB.505763.19   P-type H+-ATPase, putative
Trypanosoma cruzi TcCLB.505763.10   proton motive ATPase 1, putative
Trypanosoma cruzi TcCLB.506649.20   P-type H+-ATPase, putative
Toxoplasma gondii TGME49_252640   P-type ATPase PMA1
Toxoplasma gondii TGME49_284602   E1-E2 ATPase subfamily protein

Essentiality

Tb927.10.12500 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.12510 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.12510 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.12510 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.12510 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.12500 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.12500 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.12500 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.12500 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b4242 Escherichia coli non-essential goodall
YGL008C Saccharomyces cerevisiae inviable yeastgenome
TGME49_284602 Toxoplasma gondii Probably non-essential sidik
TGME49_252640 Toxoplasma gondii Probably non-essential sidik
TGME49_284602 Toxoplasma gondii Essentiality uncertain sidik
TGME49_252640 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Trypanosoma cruzi P-type H+-ATPase, putative Compounds References
Trypanosoma cruzi P-type H+-ATPase, putative Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Trypanosoma cruzi P-type H+-ATPase, putative 259 aa 83.3% 239 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0056 0.2665 0.4256
0.0155 0.3003 0.5887
0.0579 0.2573 0.4686
0.0071 0.2665 0.3012
0.0572 0.2519 0.2943
0.0131 0.2665 0.3012
0.0309 0.3779 0.5887
0.0463 0.2502 0.2935
0.0421 0.3535 0.5857
0.0508 0.2509 0.2938
0.0632 0.3968 0.6198
0.0328 0.3451 0.629
0.0653 0.3216 0.4686
0.055 0.2515 0.2941
0.026 0.2617 0.616
0.0562 0.2583 0.2338
0.0516 0.2518 0.2943
0.0065 0.2665 0.3012
0.0062 0.2563 0.4515
0.0129 0.2665 0.3012
0.0513 0.3141 0.6114
0.0478 0.2512 0.294
0.0537 0.2516 0.2942
0.04 0.3779 0.5865
0.004 0.2983 0.4997
0.0395 0.3087 0.6282
0.0079 0.2697 0.4686
0.0563 0.2561 0.2942
0.0328 0.3451 0.629
0.0533 0.2818 0.4686
0.0562 0.2583 0.2338
0.082 0.344 0.4686
0.0345 0.4063 0.6194
0.0533 0.2818 0.4686
0.0253 0.3282 0.4686
0.0151 0.2665 0.3667
0.0139 0.3819 0.6123
0.0562 0.2583 0.2338
0.0151 0.2665 0.3667
0.0503 0.2501 0.2934
0.0095 0.3033 0.3764
0.0103 0.3548 0.4686
0.0614 0.3209 0.4686
0.0447 0.2551 0.2937
0.0131 0.2665 0.3012
0.0426 0.2844 0.4686
0.0168 0.2665 0.3012
0.068 0.4136 0.5874
0.0053 0.2613 0.3582
0.0502 0.2513 0.294
0.0047 0.2697 0.4686
0.0373 0.3775 0.5864
0.0421 0.3535 0.5857
0.0562 0.2583 0.2338
0.0513 0.3141 0.6114
0.0542 0.2511 0.2939
0.0241 0.327 0.5887
0.0093 0.2665 0.3383
0.0069 0.2697 0.4686
0.0456 0.2537 0.2929
0.0306 0.2665 0.3012
0.0139 0.3345 0.4686
0.0562 0.2559 0.2942
0.0095 0.3033 0.3764
0.0508 0.2509 0.2938
0.0328 0.3451 0.629
0.0079 0.2697 0.4686
0.0328 0.3451 0.629
0.0011 0.2665 0.3012
0.0478 0.2501 0.2934
0.0184 0.2697 0.4686
0.0373 0.3775 0.5864
0.0328 0.3451 0.629
0.0536 0.3441 0.4686
0.0672 0.2536 0.2952
0.0479 0.3193 0.4686
0.0381 0.315 0.4686
0.0138 0.2631 0.3119
0.0529 0.3194 0.4686
0.0597 0.3103 0.4686
0.0562 0.2583 0.2338
0.0562 0.2559 0.2942
0.0489 0.2743 0.4686
0.047 0.2501 0.2934
0.0447 0.2551 0.2937
0.0602 0.2524 0.2946
0.0626 0.2535 0.2951
0.0617 0.3582 0.5864
0.0504 0.3187 0.4686
0.0103 0.3548 0.4686
0.0077 0.2665 0.3012
0.0179 0.3747 0.5887
0.0135 0.3283 0.6082
0.0545 0.3603 0.5869
0.0021 0.2665 0.3012
0.0064 0.2665 0.3012
0.0615 0.3209 0.4686
0.009 0.3151 0.5285
0.0102 0.2665 0.3012
0.0328 0.3451 0.629
0.0077 0.3345 0.4686
0.007 0.291 0.4951
0.0572 0.2519 0.2943

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.10.12500 (Trypanosoma brucei), P-type H+-ATPase, putative
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