Detailed view for LmjF.08.1040

Basic information

TDR Targets ID: 21112
Leishmania major, cathepsin L-like protease
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.1316 | Length (AA): 348 | MW (Da): 37800 | Paralog Number: 8

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00112   Papain family cysteine protease
PF08246   Cathepsin propeptide inhibitor domain (I29)

Gene Ontology

Mouse over links to read term descriptions.
GO:0008234   cysteine-type peptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

Leishmaniasis (KEGG)

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
27 340 1cs8 (A) 1 220 34.00 0 1 1.33 -0.85
127 331 1me3 (A) 2 202 61.00 0 1 1.38 -0.95
128 341 1m6d (A) 3 212 42.00 0 1 1.19 -1.19
26 346 5egw (A) 28 350 29.00 0.00000000022 1 1.26031 -0.31
127 340 3i06 (A) 2 214 60.00 0 1 1.43734 -1.41
127 340 2p86 (A) 2 214 61.00 0 1 1.45534 -1.49

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_126607)

Species Accession Gene Product
Arabidopsis thaliana AT3G54940   Papain family cysteine protease
Arabidopsis thaliana AT1G20850   xylem cysteine proteinase 2
Arabidopsis thaliana AT5G50260   KDEL-tailed cysteine endopeptidase CEP1
Arabidopsis thaliana AT4G23520   putative cysteine proteinase
Arabidopsis thaliana AT4G39090   cysteine proteinase RD19a
Arabidopsis thaliana AT1G06260   Cysteine proteinases superfamily protein
Arabidopsis thaliana AT3G19390   Granulin repeat cysteine protease family protein
Arabidopsis thaliana AT5G43060   Granulin repeat cysteine protease family protein
Arabidopsis thaliana AT3G48340   KDEL-tailed cysteine endopeptidase CEP2
Arabidopsis thaliana AT1G47128   cysteine proteinase RD21a
Arabidopsis thaliana AT4G16190   papain family cysteine protease
Arabidopsis thaliana AT2G21430   Papain family cysteine protease
Arabidopsis thaliana AT5G45890   cysteine protease SAG12
Brugia malayi Bm1_53615   Cathepsin L-like precursor
Brugia malayi Bm1_00065   Cathepsin L-like precursor
Brugia malayi Bm1_19970   Cathepsin L-like precursor
Brugia malayi Bm1_20385   Cathepsin L-like precursor
Brugia malayi Bm1_26865   Cathepsin L-like cysteine proteinase
Brugia malayi Bm1_00870   cathepsin L-like precursor
Brugia malayi Bm1_19965   Cathepsin L-like precursor
Brugia malayi Bm1_16635   Papain family cysteine protease containing protein
Brugia malayi Bm1_02100   Cathepsin L-like precursor
Brugia malayi Bm1_53625   Cathepsin L-like precursor
Brugia malayi Bm1_13265   Cathepsin L-like precursor
Caenorhabditis elegans CELE_F41E6.6   Protein TAG-196
Caenorhabditis elegans CELE_T03E6.7   Protein CPL-1, isoform B
Caenorhabditis elegans CELE_R09F10.1   Protein R09F10.1
Cryptosporidium hominis Chro.60563   cryptopain precursor
Cryptosporidium parvum cgd6_4880   cryptopain - cysteine proteinase secreted, possible transmembrane domain near N-terminus
Dictyostelium discoideum DDB_G0278721   cysteine protease 4
Dictyostelium discoideum DDB_G0274385   hypothetical protein
Dictyostelium discoideum DDB_G0290957   cysteine proteinase 1
Dictyostelium discoideum DDB_G0272298   hypothetical protein
Dictyostelium discoideum DDB_G0279185   cysteine proteinase
Dictyostelium discoideum DDB_G0272815   cysteine proteinase 5 precursor
Dictyostelium discoideum DDB_G0279187   cysteine proteinase
Dictyostelium discoideum DDB_G0283867   cysteine proteinase 3
Dictyostelium discoideum DDB_G0278401   hypothetical protein
Dictyostelium discoideum DDB_G0291191   cysteine protease
Dictyostelium discoideum DDB_G0279799   cysteine protease
Drosophila melanogaster Dmel_CG6692   Cysteine proteinase-1
Drosophila melanogaster Dmel_CG12163   CG12163 gene product from transcript CG12163-RB
Echinococcus granulosus EgrG_000989200   cathepsin l cysteine peptidase
Echinococcus granulosus EgrG_000654500   cathepsin L cysteine proteinase
Echinococcus granulosus EgrG_002067400   cathepsin l1
Echinococcus granulosus EgrG_000654800   cathepsin l1
Entamoeba histolytica EHI_159610   cysteine protease, putative
Entamoeba histolytica EHI_168240   cysteine proteinase, putative
Entamoeba histolytica EHI_151440   cysteine proteinase, putative
Entamoeba histolytica EHI_050570   cysteine proteinase, putative
Echinococcus multilocularis EmuJ_000654200   cathepsin L cysteine protease
Echinococcus multilocularis EmuJ_000989200   cathepsin l cysteine peptidase
Echinococcus multilocularis EmuJ_000654100   cathepsin l cysteine peptidase
Echinococcus multilocularis EmuJ_000654600   cysteine protease
Echinococcus multilocularis EmuJ_000654800   cathepsin L cysteine protease
Echinococcus multilocularis EmuJ_000654500   cysteine protease
Homo sapiens ENSG00000143387   cathepsin K
Homo sapiens ENSG00000136943   cathepsin V
Homo sapiens ENSG00000174080   cathepsin F
Homo sapiens ENSG00000135047   cathepsin L
Homo sapiens ENSG00000163131   cathepsin S
Leishmania braziliensis LbrM.08.0810   cathepsin L-like protease
Leishmania braziliensis LbrM.08.0830   cathepsin L-like protease
Leishmania braziliensis LbrM.19.1690   cysteine peptidase A (CPA)
Leishmania braziliensis LbrM.08.0820   cathepsin L-like protease
Leishmania donovani LdBPK_191460.1   cysteine peptidase A (CBA)
Leishmania infantum LinJ.19.1460   cysteine peptidase A (CPA)
Leishmania infantum LinJ.08.0960   cathepsin L-like protease
Leishmania infantum LinJ.08.0950   cathepsin L-like protease
Leishmania major LmjF.08.1030   cathepsin L-like protease
Leishmania major LmjF.08.1010   cathepsin L-like protease
Leishmania major LmjF.19.1420   cysteine peptidase A (CPA)
Leishmania major LmjF.08.1070   cathepsin L-like protease
Leishmania major LmjF.08.1020   cathepsin L-like protease
Leishmania major LmjF.08.1040   cathepsin L-like protease
Leishmania major LmjF.08.1080   cathepsin L-like protease
Leishmania major LmjF.08.1050   cathepsin L-like protease
Leishmania major LmjF.08.1060   cathepsin L-like protease
Leishmania mexicana LmxM.08.1040b  
Leishmania mexicana LmxM.08.1040c  
Leishmania mexicana LmxM.08.1030a  
Leishmania mexicana LmxM.08.1070partial  
Leishmania mexicana LmxM.08.1080   cathepsin L-like protease, putative
Leishmania mexicana LmxM.08.1040  
Leishmania mexicana LmxM.08.1070   cathepsin L-like protease, putative
Leishmania mexicana LmxM.19.1420   cysteine peptidase A (CBA)
Loa Loa (eye worm) LOAG_09865   cysteine protease
Loa Loa (eye worm) LOAG_11608   fibroinase
Loa Loa (eye worm) LOAG_01434   papain family cysteine protease containing protein
Loa Loa (eye worm) LOAG_12600   Papain cysteine protease/cathepsin propeptide inhibitor-containing protein
Loa Loa (eye worm) LOAG_07188   ctsf protein
Loa Loa (eye worm) LOAG_15105   hypothetical protein
Loa Loa (eye worm) LOAG_12602   hypothetical protein
Loa Loa (eye worm) LOAG_12683   hypothetical protein
Loa Loa (eye worm) LOAG_15647   hypothetical protein
Mus musculus ENSMUSG00000021477   cathepsin L
Mus musculus ENSMUSG00000042243   cDNA sequence BC051665
Mus musculus ENSMUSG00000038642   cathepsin S
Mus musculus ENSMUSG00000083282   cathepsin F
Mus musculus ENSMUSG00000056728   cathepsin L-like 3
Neospora caninum NCLIV_004380   Cathepsin L, related
Oryza sativa 4343231   Os07g0480900
Oryza sativa 4337223   Os04g0650000
Oryza sativa 4324982   Os01g0907600
Oryza sativa 4337353   Os04g0670500
Oryza sativa 4329339   Os02g0469600
Oryza sativa 4337582   Os05g0108600
Oryza sativa 4346292   Os08g0556900
Oryza sativa 4337351   Os04g0670200
Oryza sativa 4350216   Os11g0255300
Oryza sativa 4335455   Os04g0311400
Onchocerca volvulus OVOC3316  
Onchocerca volvulus OVOC1748   Cathepsin L homolog
Onchocerca volvulus OVOC12825  
Onchocerca volvulus OVOC3332  
Onchocerca volvulus OVOC5840a  
Onchocerca volvulus OVOC5843  
Onchocerca volvulus OVOC12274  
Onchocerca volvulus OVOC1713a   Cathepsin L homolog
Onchocerca volvulus OVOC11164a   Cathepsin F homolog
Onchocerca volvulus OVOC5840b  
Onchocerca volvulus OVOC7265  
Plasmodium berghei PBANKA_0932400   berghepain-2
Plasmodium falciparum PF3D7_1115300   cysteine proteinase falcipain 2b
Plasmodium falciparum PF3D7_1115400   cysteine proteinase falcipain 3
Plasmodium falciparum PF3D7_1115700   cysteine proteinase falcipain 2a
Plasmodium knowlesi PKNH_0912800   knowpain-4
Plasmodium knowlesi PKNH_0912900   knowpain-3
Plasmodium knowlesi PKNH_0913000   knowpain-2
Plasmodium vivax PVX_091415   vivapain-2
Plasmodium vivax PVX_091405   vivapain-2
Plasmodium yoelii PY00783   berghepain-2
Schistosoma japonicum Sjp_0107700   ko:K01373 cathepsin F [EC3.4.22.41], putative
Schistosoma japonicum Sjp_0005730   expressed protein
Schistosoma japonicum Sjp_0027020   Cathepsin L-like proteinase precursor, putative
Schistosoma mansoni Smp_034410.3   cathepsin F (C01 family)
Schistosoma mansoni Smp_034410.2   cathepsin F (C01 family)
Schistosoma mansoni Smp_034410.1   cathepsin F (C01 family)
Schmidtea mediterranea mk4.000002.39   Cathepsin L
Schmidtea mediterranea mk4.003459.01  
Schmidtea mediterranea mk4.000734.08   Cathepsin L
Schmidtea mediterranea mk4.002220.08   Cathepsin L
Schmidtea mediterranea mk4.023811.00   Cathepsin L
Schmidtea mediterranea mk4.003111.02  
Schmidtea mediterranea mk4.001447.00   Cathepsin L
Schmidtea mediterranea mk4.001924.09  
Schmidtea mediterranea mk4.001294.09  
Schmidtea mediterranea mk4.000389.00  
Schmidtea mediterranea mk4.026877.00   Cathepsin L
Schmidtea mediterranea mk4.000136.09   Cathepsin L
Schmidtea mediterranea mk4.000115.03  
Schmidtea mediterranea mk4.014091.00   Cathepsin F
Schmidtea mediterranea mk4.002298.04  
Schmidtea mediterranea mk4.017376.00   Cathepsin L
Schmidtea mediterranea mk4.001546.00  
Schmidtea mediterranea mk4.008390.00  
Schmidtea mediterranea mk4.033576.00   Cathepsin L
Schmidtea mediterranea mk4.016876.00   Cathepsin L
Schmidtea mediterranea mk4.001294.08   Cathepsin L
Schmidtea mediterranea mk4.001447.01  
Schmidtea mediterranea mk4.002220.04  
Schmidtea mediterranea mk4.019799.01   Cathepsin F
Schmidtea mediterranea mk4.020982.01  
Schmidtea mediterranea mk4.005973.01   Cathepsin L
Schmidtea mediterranea mk4.001447.09   Cathepsin L
Schmidtea mediterranea mk4.001075.04   Cathepsin L
Schmidtea mediterranea mk4.002298.05   Cathepsin L
Schmidtea mediterranea mk4.000002.11  
Schmidtea mediterranea mk4.001447.10   Cathepsin L
Schmidtea mediterranea mk4.000734.07  
Schmidtea mediterranea mk4.000136.08  
Schmidtea mediterranea mk4.040357.00   Cathepsin L
Schmidtea mediterranea mk4.001924.01  
Trypanosoma brucei gambiense Tbg972.6.725   cysteine peptidase precursor,cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma brucei gambiense Tbg972.6.735   cysteine peptidase precursor,cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma brucei gambiense Tbg972.6.760   cysteine peptidase precursor,cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma brucei Tb927.6.1040   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma brucei Tb927.6.1010   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma brucei Tb927.6.970   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma brucei Tb927.6.1000   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma brucei Tb927.6.990   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma brucei Tb927.6.1060   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma brucei Tb927.6.1030   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma brucei Tb927.6.960   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma brucei Tb927.6.980   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma brucei Tb927.6.1020   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma brucei Tb927.6.1050   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma congolense TcIL3000_0_28390   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma congolense TcIL3000_0_53250   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma congolense TcIL3000_0_18880   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma congolense TcIL3000_0_28270   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma congolense TcIL3000_0_31720   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma congolense TcIL3000_0_12120   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma congolense TcIL3000_0_17860   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma congolense TcIL3000_0_60020   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma congolense TcIL3000_0_31730   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma congolense TcIL3000_0_55820   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma congolense TcIL3000_0_37240   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma congolense TcIL3000_0_26780   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma congolense TcIL3000_0_55780   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma congolense TcIL3000_0_25670   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma congolense TcIL3000_0_44210   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma congolense TcIL3000_0_11840   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma congolense TcIL3000_0_55830   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma congolense TcIL3000_0_49190   cysteine peptidase, Clan CA, family C1, Cathepsin L-like
Trypanosoma cruzi TcCLB.507603.260   cysteine peptidase, putative
Trypanosoma cruzi TcCLB.507603.270   cruzipain precursor, putative
Trypanosoma cruzi TcCLB.509429.320   cysteine peptidase, putative
Trypanosoma cruzi TcCLB.509401.30   cysteine peptidase, putative
Trypanosoma cruzi TcCLB.507537.20   cysteine peptidase, putative
Trypanosoma cruzi TcCLB.509401.20   cysteine peptidase, clan CA, family C1, cathepsin L-like, putative
Toxoplasma gondii TGME49_321530   cathepsin CPL
Theileria parva TP02_0098   cysteine protease, putative
Theileria parva TP03_0283   cysteine proteinase, putative
Theileria parva TP03_0281   cysteine proteinase, putative
Theileria parva TP02_0097   cysteine protease, putative
Theileria parva TP03_0285   cysteine proteinase
Theileria parva TP02_0099   cysteine protease, putative
Theileria parva TP03_0282   cysteine proteinase, putative
Trichomonas vaginalis TVAG_151460   Clan CA, family C1, cathepsin L or K-like cysteine peptidase
Trichomonas vaginalis TVAG_202090   Clan CA, family C1, cathepsin L-like cysteine peptidase
Trichomonas vaginalis TVAG_003650   Clan CA, family C1, papain-like cysteine peptidase
Trichomonas vaginalis TVAG_057000   Clan CA, family C1, papain-like cysteine peptidase
Trichomonas vaginalis TVAG_164120   Clan CA, family C1, cathepsin L-like cysteine peptidase
Trichomonas vaginalis TVAG_034140   Clan CA, family C1, papain-like cysteine peptidase
Trichomonas vaginalis TVAG_427130   Clan CA, family C1, cathepsin L-like cysteine peptidase
Trichomonas vaginalis TVAG_484350   Clan CA, family C1, cathepsin L-like cysteine peptidase
Trichomonas vaginalis TVAG_467970   Clan CA, family C1, cathepsin L-like cysteine peptidase
Trichomonas vaginalis TVAG_090100   Clan CA, family C1, papain-like cysteine peptidase
Trichomonas vaginalis TVAG_518810   Clan CA, family C1, papain-like cysteine peptidase
Trichomonas vaginalis TVAG_043620   Clan CA, family C1, papain-like cysteine peptidase
Trichomonas vaginalis TVAG_325040   Clan CA, family C1, cathepsin L-like cysteine peptidase
Trichomonas vaginalis TVAG_135480   Clan CA, family C1, cathepsin L-like cysteine peptidase
Trichomonas vaginalis TVAG_228450   Clan CA, family C1, cathepsin L-like cysteine peptidase
Trichomonas vaginalis TVAG_218830   Clan CA, family C1, cathepsin L-like cysteine peptidase
Trichomonas vaginalis TVAG_096740   Clan CA, family C1, papain-like cysteine peptidase
Trichomonas vaginalis TVAG_410260   Clan CA, family C1, cathepsin L-like cysteine peptidase
Trichomonas vaginalis TVAG_465470   Clan CA, family C1, cathepsin L-like cysteine peptidase
Trichomonas vaginalis TVAG_267850   Clan CA, family C1, cathepsin L or K-like cysteine peptidase
Trichomonas vaginalis TVAG_053440   Clan CA, family C1, papain-like cysteine peptidase
Trichomonas vaginalis TVAG_427120   Clan CA, family C1, cathepsin L-like cysteine peptidase
Trichomonas vaginalis TVAG_437820   Clan CA, family C1, cathepsin L-like cysteine peptidase
Trichomonas vaginalis TVAG_298080   Clan CA, family C1, cathepsin L-like cysteine peptidase
Trichomonas vaginalis TVAG_405280   Clan CA, family C1, cathepsin L-like cysteine peptidase
Trichomonas vaginalis TVAG_047960   Clan CA, family C1, papain-like cysteine peptidase
Trichomonas vaginalis TVAG_355480   Clan CA, family C1, cathepsin L-like cysteine peptidase

Essentiality

LmjF.08.1040 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.1000 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.1000 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.6.1000 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.1000 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.6.960 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.960 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.6.960 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.960 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_T03E6.7 Caenorhabditis elegans embryonic lethal wormbase
CELE_T03E6.7 Caenorhabditis elegans larval lethal wormbase
CELE_T03E6.7 Caenorhabditis elegans slow growth wormbase
TGME49_321530 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania mexicana 337527   561727   585910  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 4174-73-6. chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in cell assay; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 7179420
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania mexicana 337527   561727   585910  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 4174-73-6. chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in cell assay; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 7179420
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from enzyme assay (ECO:0000005) Leishmania mexicana 337527   561727   585910  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 4174-73-6. chemical inhibition with known cysteine peptidase inhibitors leads to reduced enzyme activity in vitro; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 7179420
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania amazonensis No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: L-Amino acid esters appear to be hydrolyzed by cysteine peptidase and lead to slow growth of Leishmania sp. in cell assay; . References: 1428504 2235078 2345655
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in cell assay; . References: 2270108
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from enzyme assay (ECO:0000005) Leishmania amazonensis 81384   576750  
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with known cysteine peptidase inhibitors leads to reduced enzyme activity in vitro; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 8023752
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from enzyme assay (ECO:0000005) Leishmania major 81384   576750  
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with known cysteine peptidase inhibitors leads to reduced enzyme activity in vitro; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 8023752
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania pifanoi No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in cell assay; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 7891736
growth (GO:0040007) normal (PATO:0000461) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from loss-of-function mutant phenotype (ECO:0000016) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: LmCPb (genetic tandem array) null mutant generated without affecting growth or differentiation, but reducing virulence by ~80% (most parasites die post-invasion of macrophages); lesions still grow slowly in vivo, but proves LmCPbs are non-essential for parasite survival; LmCPa/LmCPb double-null mutant cannot generate lesions in vivo; attenuation with LmCPa/LmCPb double-null mutant leads to decreased growth of wild-type (partial vaccine); however, different species may have different interactions/redundancies of CPs, so therapy is still possible via CP inhibition; LMCPB_LEIME; . References: 16216395 16803590 8650210 9162063 9862710
growth (GO:0040007) normal (PATO:0000461) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from loss-of-function mutant phenotype (ECO:0000016) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: LmCPb (genetic tandem array) null mutant generated without affecting growth or differentiation, but reducing virulence by ~80% (most parasites die post-invasion of macrophages); lesions still grow slowly in vivo, but proves LmCPbs are non-essential for parasite survival; LmCPa/LmCPb double-null mutant cannot generate lesions in vivo; attenuation with LmCPa/LmCPb double-null mutant leads to decreased growth of wild-type (partial vaccine); however, different species may have different interactions/redundancies of CPs, so therapy is still possible via CP inhibition; LMCPB_LEIME; . References: 16216395 16803590 8650210 9162063 9862710
growth (GO:0040007) normal (PATO:0000461) single cell organism (CARO:0000064) metacyclic form (BTO:0002487) inferred from loss-of-function mutant phenotype (ECO:0000016) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: LmCPb (genetic tandem array) null mutant generated without affecting growth or differentiation, but reducing virulence by ~80% (most parasites die post-invasion of macrophages); lesions still grow slowly in vivo, but proves LmCPbs are non-essential for parasite survival; LmCPa/LmCPb double-null mutant cannot generate lesions in vivo; attenuation with LmCPa/LmCPb double-null mutant leads to decreased growth of wild-type (partial vaccine); however, different species may have different interactions/redundancies of CPs, so therapy is still possible via CP inhibition; LMCPB_LEIME; . References: 16216395 16803590 8650210 9162063 9862710
viability (PATO:0000169) decreased (PATO:0000468) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from loss-of-function mutant phenotype (ECO:0000016) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: LmCPb (genetic tandem array) null mutant generated without affecting growth or differentiation, but reducing virulence by ~80% (most parasites die post-invasion of macrophages); lesions still grow slowly in vivo, but proves LmCPbs are non-essential for parasite survival; LmCPa/LmCPb double-null mutant cannot generate lesions in vivo; attenuation with LmCPa/LmCPb double-null mutant leads to decreased growth of wild-type (partial vaccine); however, different species may have different interactions/redundancies of CPs, so therapy is still possible via CP inhibition; LMCPB_LEIME; . References: 16216395 16803590 8650210 9162063 9862710
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in cell assay; LMCPB_LEIME; . References: 11072899
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from in-silico analysis (ECO:0000043) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with known cysteine peptidase inhibitors leads to reduced enzyme activity in vitro; LMCPB_LEIME; . References: 11231271
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from enzyme assay (ECO:0000005) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with known cysteine peptidase inhibitors leads to reduced enzyme activity in vitro; LMCPB_LEIME; . References: 11231271
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) host (GO:0018995) Leishmania donovani No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 81989-95-9. chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in vivo (balb/c mouse) and cure of parasite burden; also provided protection against further infection (partial vaccine); General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 11238649
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania donovani No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 81989-95-9. chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in vivo (balb/c mouse) and cure of parasite burden; also provided protection against further infection (partial vaccine); General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 11238649
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) host (GO:0018995) Leishmania infantum No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 67776-06-1. chemical inhibition with NO-donor inhibitors leads to slow growth of Leishmania sp. in vivo (balb/c mouse); . References: 11342060
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania infantum No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 67776-06-1. chemical inhibition with NO-donor inhibitors leads to slow growth of Leishmania sp. in vivo (balb/c mouse); . References: 11342060
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania infantum 36285   106963   542659   542660   545056   571140   571141   571142  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 67776-06-1; Drug: 138472-01-2. chemical inhibition with NO-donor inhibitors leads to slow growth of Leishmania sp. in cell assay; . References: 11342060 14975759
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from enzyme assay (ECO:0000005) Leishmania infantum 36285   106963   542659   542660   545056   549058   549059   571140   571141   571142  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 67776-06-1; Drug: 138472-01-2. chemical inhibition with NO-donor inhibitors leads to reduced enzyme activity in vitro; . References: 11342060 14975759
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from in-silico analysis (ECO:0000043) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with peptide inhibitors and peptidomimetics leads to reduced enzyme activity in vitro; LMCPB_LEIME; . References: 11356516 11463460 11549362 11606223 11828405 11985465
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from enzyme assay (ECO:0000005) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with peptide inhibitors and peptidomimetics leads to reduced enzyme activity in vitro; LMCPB_LEIME; . References: 11356516 11463460 11549362 11606223 11828405 11985465
growth (GO:0040007) increased (PATO:0000470) single cell organism (CARO:0000064) amastigote (BTO:0000062) host (GO:0018995) Leishmania major No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 215098-90-1. chemical inhibition with known cysteine peptidase inhibitors leads to increased growth of Leishmania sp. in vivo (balb/c mouse) and exacerbation of infection through moderation of host immune response; . References: 11470767
growth (GO:0040007) increased (PATO:0000470) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania major No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 215098-90-1. chemical inhibition with known cysteine peptidase inhibitors leads to increased growth of Leishmania sp. in vivo (balb/c mouse) and exacerbation of infection through moderation of host immune response; . References: 11470767
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with peptide inhibitors and peptidomimetics leads to slow growth of Leishmania sp. in cell assay; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 11985465
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from in-silico analysis (ECO:0000043) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with peptide inhibitors and peptidomimetic triazoles leads to reduced enzyme activity in vitro; LMCPB_LEIME; . References: 11985465 15132590
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from enzyme assay (ECO:0000005) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with peptide inhibitors and peptidomimetic triazoles leads to reduced enzyme activity in vitro; LMCPB_LEIME; . References: 11985465 15132590
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) host (GO:0018995) Leishmania tropica No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 233277-99-1. chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in vivo (balb/c mouse lesions) and reduced lesion size; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 15172223
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania tropica No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 233277-99-1. chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in vivo (balb/c mouse lesions) and reduced lesion size; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 15172223
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania tropica No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 233277-99-1. chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in cell assay; inhibitors overcome redundancy?; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 15172223
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania donovani 0   6693   81384   236103   327961   376038   543486   543487   575389   575390   575391   576750  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 22386-37-4; Drug: 36677-78-8; Drug: 52090-11-6; Drug: 98319-30-3; Drug: 300670-24-0; Drug: 302939-54-4; Drug: 303216-44-6; Drug: 309742-24-3; Drug: 312280-89-0; Drug: 315703-20-9; Drug: 320369-30-0; Drug: 322409-94-9; Drug: 330220-86-5; Drug: 335206-77-4; Drug: 347910-28-5; Drug: 352560-86-2; Drug: 363180-61-4; Drug: 413580-11-7; Drug: 416878-80-3; Drug: 419547-26-5; Drug: 428852-79-3; Drug: 825612-08-6; Drug: 825612-09-7; Drug: 825612-10-0. in silico virtual screening of falcipains lead to bioactive compounds against Leishmania promastigotes; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 15588096
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from in-silico analysis (ECO:0000043) Leishmania donovani 0   6693   81384   236103   327961   376038   543486   543487   575389   575390   575391   576750  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 22386-37-4; Drug: 36677-78-8; Drug: 52090-11-6; Drug: 98319-30-3; Drug: 300670-24-0; Drug: 302939-54-4; Drug: 303216-44-6; Drug: 309742-24-3; Drug: 312280-89-0; Drug: 315703-20-9; Drug: 320369-30-0; Drug: 322409-94-9; Drug: 330220-86-5; Drug: 335206-77-4; Drug: 347910-28-5; Drug: 352560-86-2; Drug: 363180-61-4; Drug: 413580-11-7; Drug: 416878-80-3; Drug: 419547-26-5; Drug: 428852-79-3; Drug: 825612-08-6; Drug: 825612-09-7; Drug: 825612-10-0. in silico virtual screening of falcipains lead to bioactive compounds against cysteine protease in vitro; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 15588096
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from specific protein inhibition (ECO:0000020) Leishmania donovani 0   6693   81384   236103   327961   376038   543486   543487   575389   575390   575391   576750  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 22386-37-4; Drug: 36677-78-8; Drug: 52090-11-6; Drug: 98319-30-3; Drug: 300670-24-0; Drug: 302939-54-4; Drug: 303216-44-6; Drug: 309742-24-3; Drug: 312280-89-0; Drug: 315703-20-9; Drug: 320369-30-0; Drug: 322409-94-9; Drug: 330220-86-5; Drug: 335206-77-4; Drug: 347910-28-5; Drug: 352560-86-2; Drug: 363180-61-4; Drug: 413580-11-7; Drug: 416878-80-3; Drug: 419547-26-5; Drug: 428852-79-3; Drug: 825612-08-6; Drug: 825612-09-7; Drug: 825612-10-0. in silico virtual screening of falcipains lead to bioactive compounds against cysteine protease in vitro; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 15588096
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from specific protein inhibition (ECO:0000020) Leishmania infantum No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 186409-04-1. chemical inhibition with NO-donor inhibitors leads to reduced enzyme activity in vitro; . References: 15588704
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania donovani No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 2935-91-3; Drug: 24117-97-3; Drug: 98647-23-5; Drug: 198124-18-4; Drug: 256238-87-6; Drug: 257287-52-8; Drug: 263157-80-8; Drug: 294854-43-6; Drug: 303124-84-7; Drug: 303147-38-8; Drug: 321579-84-4; Drug: 331247-87-1; Drug: 337923-53-2; Drug: 338791-39-2; Drug: 675828-74-7; Drug: 676226-56-5; Drug: 678967-57-2; Drug: 681213-40-1; Drug: 686272-33-3; Drug: 696586-43-3; Drug: 705250-31-3; Drug: 708987-83-1; Drug: 831234-40-3; Drug: 882161-95-7; Drug: 882161-98-0; Drug: 882161-99-1; Drug: 882162-00-7; Drug: 882162-01-8; Drug: 882162-02-9; Drug: 882162-03-0; Drug: 882162-04-1; Drug: 882162-05-2; Drug: 882162-06-3; Drug: 882162-07-4; Drug: 882162-08-5; Drug: 882162-09-6; Drug: 882162-10-9; Drug: 882162-11-0; Drug: 882162-12-1; Drug: 882162-13-2; Drug: 882162-14-3. in silico virtual screening of falcipains lead to bioactive compounds against Leishmania promastigotes; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 16509575
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from in-silico analysis (ECO:0000043) Leishmania donovani No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 2935-91-3; Drug: 24117-97-3; Drug: 98647-23-5; Drug: 198124-18-4; Drug: 256238-87-6; Drug: 257287-52-8; Drug: 263157-80-8; Drug: 294854-43-6; Drug: 303124-84-7; Drug: 303147-38-8; Drug: 321579-84-4; Drug: 331247-87-1; Drug: 337923-53-2; Drug: 338791-39-2; Drug: 675828-74-7; Drug: 676226-56-5; Drug: 678967-57-2; Drug: 681213-40-1; Drug: 686272-33-3; Drug: 696586-43-3; Drug: 705250-31-3; Drug: 708987-83-1; Drug: 831234-40-3; Drug: 882161-95-7; Drug: 882161-98-0; Drug: 882161-99-1; Drug: 882162-00-7; Drug: 882162-01-8; Drug: 882162-02-9; Drug: 882162-03-0; Drug: 882162-04-1; Drug: 882162-05-2; Drug: 882162-06-3; Drug: 882162-07-4; Drug: 882162-08-5; Drug: 882162-09-6; Drug: 882162-10-9; Drug: 882162-11-0; Drug: 882162-12-1; Drug: 882162-13-2; Drug: 882162-14-3. in silico virtual screening of falcipains lead to bioactive compounds against cysteine protease in vitro; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 16509575
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from specific protein inhibition (ECO:0000020) Leishmania donovani No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 2935-91-3; Drug: 24117-97-3; Drug: 98647-23-5; Drug: 198124-18-4; Drug: 256238-87-6; Drug: 257287-52-8; Drug: 263157-80-8; Drug: 294854-43-6; Drug: 303124-84-7; Drug: 303147-38-8; Drug: 321579-84-4; Drug: 331247-87-1; Drug: 337923-53-2; Drug: 338791-39-2; Drug: 675828-74-7; Drug: 676226-56-5; Drug: 678967-57-2; Drug: 681213-40-1; Drug: 686272-33-3; Drug: 696586-43-3; Drug: 705250-31-3; Drug: 708987-83-1; Drug: 831234-40-3; Drug: 882161-95-7; Drug: 882161-98-0; Drug: 882161-99-1; Drug: 882162-00-7; Drug: 882162-01-8; Drug: 882162-02-9; Drug: 882162-03-0; Drug: 882162-04-1; Drug: 882162-05-2; Drug: 882162-06-3; Drug: 882162-07-4; Drug: 882162-08-5; Drug: 882162-09-6; Drug: 882162-10-9; Drug: 882162-11-0; Drug: 882162-12-1; Drug: 882162-13-2; Drug: 882162-14-3. in silico virtual screening of falcipains lead to bioactive compounds against cysteine protease in vitro; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 16509575
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania major No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 872361-01-8; Drug: 872361-02-9; Drug: 886061-72-9; Drug: 886061-73-0; Drug: 886061-74-1; Drug: 886061-75-2; Drug: 886061-76-3; Drug: 886061-77-4; Drug: 886061-78-5; Drug: 886061-79-6; Drug: 886061-80-9; Drug: 886061-81-0; Drug: 886061-82-1; Drug: 886061-85-4; Drug: 886061-86-5; Drug: 886061-87-6; Drug: 886061-88-7; Drug: 886061-89-8; Drug: 886061-90-1; Drug: 886061-91-2; Drug: 886061-92-3; Drug: 886061-93-4; Drug: 886061-99-0; Drug: 886062-00-6; Drug: 886062-05-1; Drug: 886062-06-2; Drug: 886062-07-3; Drug: 886062-08-4; Drug: 886062-09-5; Drug: 886062-10-8; Drug: 886062-11-9; Drug: 886062-12-0; Drug: 886062-13-1; Drug: 886062-14-2; Drug: 886062-15-3; Drug: 886062-16-4; Drug: 886062-17-5; Drug: 906674-95-1. chemical inhibition with peptidomimetics and Aziridine-2,3-dicarboxylates leads to slow growth of Leishmania sp. in cell assay; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 16801424
autophagy (GO:0006914) disrupted (PATO:0001507) single cell organism (CARO:0000064) metacyclic form (BTO:0002487) inferred from loss-of-function mutant phenotype (ECO:0000016) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: LmCPa/LmCPb double-null mutant leads to disrupts autophagy and the transition between promastigotes and metacyclics and metacyclics and amastigotes; explains reduced virulence and survivability during infection; . References: 16803590
autophagy (GO:0006914) disrupted (PATO:0001507) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from loss-of-function mutant phenotype (ECO:0000016) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: LmCPa/LmCPb double-null mutant leads to disrupts autophagy and the transition between promastigotes and metacyclics and metacyclics and amastigotes; explains reduced virulence and survivability during infection; . References: 16803590
cell differentiation (GO:0030154) disrupted (PATO:0001507) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from loss-of-function mutant phenotype (ECO:0000016) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: LmCPa/LmCPb double-null mutant leads to disrupts autophagy and the transition between promastigotes and metacyclics and metacyclics and amastigotes; explains reduced virulence and survivability during infection; . References: 16803590
cell differentiation (GO:0030154) disrupted (PATO:0001507) single cell organism (CARO:0000064) metacyclic form (BTO:0002487) inferred from loss-of-function mutant phenotype (ECO:0000016) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: LmCPa/LmCPb double-null mutant leads to disrupts autophagy and the transition between promastigotes and metacyclics and metacyclics and amastigotes; explains reduced virulence and survivability during infection; . References: 16803590
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania infantum No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with NO-donor inhibitors leads to slow growth of Leishmania sp. in cell assay; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 17018162

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

  • Validation: in vivo inhibition
  • annotated by: aaronjr@u.washington.edu.
  • References: 11342060 11470767
  • Validation: in vivo inhibition
  • annotated by: aaronjr@u.washington.edu.
  • References: 11238649 15172223

Associated compounds / Druggability

Known modulators for this target

Compound Source Reference
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens cathepsin K Compounds References
Leishmania mexicana (unnamed target) Compounds References
Trypanosoma brucei cysteine peptidase, Clan CA, family C1, Cathepsin L-like Compounds References
Rattus norvegicus Cathepsin S Compounds References
Leishmania major cathepsin L-like protease Compounds References
Trypanosoma brucei cysteine peptidase, Clan CA, family C1, Cathepsin L-like Compounds References
Bos taurus Cathepsin L Compounds References
Trypanosoma brucei cysteine peptidase, Clan CA, family C1, Cathepsin L-like Compounds References
Homo sapiens cathepsin V Compounds References
Trypanosoma brucei gambiense cysteine peptidase precursor,cysteine peptidase, Clan CA, family C1, Cathepsin L-like Compounds References
Trypanosoma brucei cysteine peptidase, Clan CA, family C1, Cathepsin L-like Compounds References
Leishmania major cathepsin L-like protease Compounds References
Trypanosoma brucei cysteine peptidase, Clan CA, family C1, Cathepsin L-like Compounds References
Plasmodium falciparum cysteine proteinase falcipain 3 Compounds References
Carica papaya Papain Compounds References
Trypanosoma brucei cysteine peptidase, Clan CA, family C1, Cathepsin L-like Compounds References
Leishmania major cathepsin L-like protease Compounds References
Canis familiaris Cathepsin K Compounds References
Leishmania major cathepsin L-like protease Compounds References
Trypanosoma brucei cysteine peptidase, Clan CA, family C1, Cathepsin L-like Compounds References
Leishmania major cathepsin L-like protease Compounds References
Trypanosoma cruzi cysteine peptidase, putative Compounds References
Leishmania donovani Cysteine protease Compounds References
Trypanosoma brucei cysteine peptidase, Clan CA, family C1, Cathepsin L-like Compounds References
Homo sapiens cathepsin L Compounds References
Plasmodium falciparum cysteine proteinase falcipain 2b Compounds References
Mus musculus cathepsin S Compounds References
Trypanosoma brucei cysteine peptidase, Clan CA, family C1, Cathepsin L-like Compounds References
Leishmania major cathepsin L-like protease Compounds References
Homo sapiens cathepsin S Compounds References
Mus musculus cathepsin L Compounds References
Leishmania major cathepsin L-like protease Compounds References
Dermatophagoides pteronyssinus Peptidase 1 Compounds References
Trypanosoma cruzi cysteine peptidase, putative Compounds References
Leishmania major cysteine peptidase A (CPA) Compounds References
Trypanosoma cruzi cruzipain precursor, putative Compounds References
Plasmodium falciparum cysteine proteinase falcipain 2a Compounds References
Rattus norvegicus Cathepsin K Compounds References
Trypanosoma brucei cysteine peptidase, Clan CA, family C1, Cathepsin L-like Compounds References
Rattus norvegicus Cathepsin L Compounds References
Trypanosoma brucei cysteine peptidase, Clan CA, family C1, Cathepsin L-like Compounds References
Oryctolagus cuniculus Cathepsin K Compounds References
Homo sapiens cathepsin F Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Schistosoma mansoni cathepsin B-like peptidase (C01 family) 345 aa 26.1% 284 aa Compounds References
Capra hircus Cathepsin H 335 aa 36.5% 342 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0249 0.2504 0.2792
0.0109 0.4956 1
0.0054 1 0.5
0.0944 0.2841 0.6028
0.0583 1 0.5
0.0117 0.562 1
0.0027 0.7888 0.5
0.0054 1 0.5
0.1152 0.4937 0.6777
0.0424 0.4894 0.8266
0.0629 0.2636 0.4231
0.0253 0.3023 0.4321
0.0101 0.6495 0.5
0.059 0.808 1
0.0848 0.6806 1
0.0328 0.6561 1
0.0027 1 0.5
0.1936 0.5037 0.7795
0.0793 0.4853 1
0.0133 1 0.5
0.123 0.3604 0.6535
0.0758 1 1
0.0054 1 0.5
0.0551 1 1
0.0214 0.255 0.2268
0.0346 0.3186 0.3172
0.0845 0.2549 0.7196
0.0086 0.2924 1
0.2225 0.4145 0.4756
0.0185 0.3483 1
0.0187 0.4158 0.5
0.0567 0.3658 1
0.0791 1 1
0.0589 0.4354 0.8573
0.0442 0.2683 0.2668
0.045 1 0.5
0.02 1 1
0.0054 1 0.5
0.0373 0.3357 0.4547
0.0345 0.4089 1
0.0486 0.5597 0.5559
0.0054 0.3656 1
0.0095 1 0.5
0.0707 0.2789 1
0.061 0.5064 0.6287
0.1168 0.3129 0.7739
0.026 1 1
0.0077 0.3559 1
0.116 1 0.5
0.0086 0.2924 1
0.0054 1 0.5
0.016 0.412 0.5459
0.0081 1 0.5
0.0113 0.4176 1
0.0218 0.3088 1
0.0339 0.271 0.271
0.0083 0.3012 1
0.0618 0.9523 1
0.0111 0.4427 0.5
0.2031 0.5122 0.5258
0.0819 0.4453 0.4401
0.0163 0.4195 1
0.0361 0.5001 1
0.0163 1 1
0.0054 1 0.5
0.0675 1 1
0.0086 0.2924 1
0.0235 0.4435 0.5
0.0022 1 0.5
0.0086 0.3216 1
0.0054 1 0.5
0.0226444 0.619525 1
0.0604 0.2561 0.2561
0.0405 1 1
0.0130612 0.288023 0.249664
0.0568 0.3035 0.3103
0.0086 0.2924 1
0.0054 1 0.5
0.0487 0.2561 0.2558
0.2046 0.3344 0.8481
0.0130612 0.288023 0.249664
0.0346 0.2565 0.2565
0.2248 0.2846 0.2904
0.0629 0.2636 0.4231
0.0489 0.6538 1
0.0198 0.3801 1
0.0026 1 0.5
0.1488 0.26 0.26
0.0069 1 1
0.1181 1 1
0.0086 0.2924 1
0.0069 0.9715 0
0.0152 0.3133 0.431
0.0392 0.3943 1
0.0818 1 0.5
0.1401 0.3495 0.6849
0.0681 1 1
0.0674 0.309 1
0.05 0.5856 1
0.0027 0.397 0.5
0.0168498 0.357323 0
0.0191 1 0.5
0.097 1 1
0.0081 1 0.5
0.0982 1 0.5
0.0142 0.2924 1
0.0054 1 0.5
0.0151 0.2569 0.9019
0.0079 0.2636 0.1586
0.061 1 1
0.0907 0.3429 0.3429
0.1406 0.4156 0.6924
0.0622 1 0.5
0.0027 1 0.5
0.0086 0.2924 1
0.0071 1 0.5
0.00440343 1 0.5
0.0598 1 0.5
0.0282 0.2699 0.2696
0.0246 1 0.5
0.133 0.3556 0.7533
0.0086 0.2924 1
0.0889 0.5911 1
0.0891 0.3178 0.5092
0.1364 0.3965 0.4718
0.0558 0.3782 1
0.0285 0.3247 1
0.0206 1 1
0.0047 0.287 0
0.024716 0.414133 0.246688
0.0067 1 0.5
0.0373 0.3357 0.4547
0.0683 0.5163 0.7535
0.1104 1 1
0.0148 0.4486 0.5
0.0069 1 0.5
0.0216 0.4176 1
0.0054 1 0.5
0.071 1 0.5
0.0719 1 0.5
0.101591 0.364677 1
0.0679 1 1
0.0279 0.2699 0.2696
0.0266 0.3485 0.3087
0.0778 0.3497 0.9577
0.0257 1 0.5
0.0098 0.2727 1
0.1274 0.3409 0.5537
0.0395 1 0.5
0.0154 0.3698 0.5
0.0947 0.3409 0.9311
0.0081 1 0.5
0.067 0.4891 1
0.0072 0.3283 0.5603
0.0086 0.2924 1
0.0841 0.4467 0.557
0.0494 1 1
0.0105 0.4176 1
0.0409 0.6682 0
0.0349 0.2554 0.9527
0.121 0.4105 0.8627
0.0154 0.5249 1
0.0371 0.3199 0.3246
0.0113 0.4176 1
0.0329 0.4058 1
0.0666 0.3918 1
0.1193 0.4456 1
0.0081 1 0.5
0.0054 1 0.5
0.151 0.401 0.3994
0.110639 1 0.5
0.0576 0.5822 1
0.0234 0.4709 0.7196
0.0087 1 0.5
0.1164 1 1
0.0699 0.3124 0.4634
0.0336 0.4346 1
0.0681 0.3946 0.658
0.0081 1 0.5
0.027 0.2656 0.2616
0.0575 1 1
0.0715 0.5394 1
0.0077 1 1
0.1252 0.3755 1
0.2594 0.4935 0.6755
0.0273 1 1
0.0935 1 0.5
0.0919 0.4641 0.3511
0.2112 0.4916 1
0.0435 1 1
0.0075 1 1
0.0519 1 0.5
0.1269 0.7319 1
0.0027 0.2683 1
0.0256 1 1
0.1157 0.5075 1
0.1635 0.2906 1
0.0202 0.4176 1
0.0054 1 0.5
0.0869 0.3175 0.7852
0.0404 0.2733 0
0.0231 0.705 1
0.0571 0.2649 0.7587
0.0478 0.3294 0.5712
0.0597 0.3106 0.4336
0.0505 0.4176 1
0.0047 1 0.5
0.0287 1 0.5
0.0059 0.2727 0.8557
0.0315 1 1
0.0568 1 0.5
0.0044 0.3553 1
0.1227 1 1
0.0086 0.2924 1
0.0054 1 0.5
0.0819 1 0.5
0.0794 0.6475 1
0.0081 1 0.5
0.0182 0.319 0.5
0.0774 1 0.5
0.0698 0.5228 1
0.0364 0.2935 0.8568
0.01 0.3132 1
0.0984 1 0.5
0.0681 0.3946 0.658
0.0209 0.4598 1
0.0462 0.4399 0.819
0.1338 0.6337 1
0.0152 0.3962 0.3766
0.0446 0.4402 0.8645
0.0184 0.2531 0.2509
0.0888 1 0.5
0.0638 0.3713 1
0.0113 0.562 1
0.1008 1 1
0.0273 1 1
0.0054 1 0.5
0.0756 0.9054 1
0.0427 0.4612 0.4563
0.0027 1 0.5
0.0086 0.2647 1
0.0428 0.5321 1
0.0632 0.4036 0.4036
0.0675 0.3484 0
0.0069 0.2924 1
0.0054 1 0.5
0.0289 0.3018 0.5262
0.0979 1 0.5
0.0113 0.4176 1
0.0044 1 0.5
0.2249 0.4186 0.3857
0.055 1 1
0.0078 0.9639 0.5
0.0786 1 1
0.0044 1 0.5
0.1331 0.2538 0.2449
0.1252 1 0.5
0.0081 1 0.5
0.0091 0.2821 1
0.1381 0.3724 0.5714
0.1422 0.7329 1
0.0535 0.5374 1
0.0078 1 1
0.0093 1 0.5
0.1192 0.394 1
0.0583 0.2507 0.2249
0.0407 0.2529 0.2523
0.0081 1 0.5
0.1208 0.4572 1
0.0604 0.8246 1
0.086 0.3295 1
0.0027 1 0.5
0.0362 0.4634 1
0.1156 0.3805 0.6649
0.0113 0.4176 1
0.0124 0.3698 0.5
0.0329 0.4058 1
0.2031 0.5122 0.5258
0.0081 0.9581 0.5
0.0027 1 0.5
0.0381 0.4195 1
0.0302 1 1
0.0407 0.684 0
0.0293 1 0.5
0.032 0.6867 1
0.1395 0.3223 0.7167
0.0198 0.2531 0.2509
0.0079 0.3969 0.5489
0.0928 1 1
0.1401 0.5189 0.967
0.0343 0.3176 0.3176
0.1657 1 1
0.0348 0.2918 0.39
0.00858541 0.416007 1
0.024 0.287 0.9057
0.008 0.2948 1
0.1218 0.7828 1
0.0313 0.3939 1
0.0238 0.595 1
0.0079 0.3523 0.4235
0.1061 0.3456 0.9433
0.0081 1 0.5
0.0086 0.2924 1
0.0483 0.3546 0.3538
0.0368 0.2749 0.3635
0.2009 0.4985 0.8029
0.0509 1 0.5
0.0071781 0.253381 0
0.2102 0.6531 1
0.0438 0.4633 1
0.0027 0.4557 0.5
0.0639 0.2548 0.7083
0.1349 0.2681 1
0.0022 0.8669 0.8669
0.0362 0.3951 0.4453
0.0758 1 1
0.0265 0.4877 0.3731
0.0266 0.8552 1
0.0322 0.3211 1
0.0054 1 0.5
0.0328 0.3641 0.3635
0.0495 0.37 0
0.0362 0.4634 1
0.1955 0.4848 1
0.182258 0.580494 0.954635
0.0112 0.562 1
0.027 0.2656 0.2616
0.0917 1 0.5
0.236 0.4739 1
0.0768 0.5151 1
0.1545 0.6303 1
0.0027 1 0.5
0.1192 0.3397 0.6851
0.0209 0.41 1
0.0048 1 0.5
0.0788 0.3398 0.9338
0.0396 1 1
0.0699 0.3124 0.4634
0.0415 0.2985 1
0.0968 1 1
0.1362 0.4435 0.7785
0.0475 0.2784 0.5004
0.0027 0.8 0
0.2384 0.5692 1
0.1946 0.435 0.4328
0.0136 0.562 1
0.074 0.4444 1
0.0109 0.4176 1
0.1545 0.6303 1
0.0103 0.2646 0
0.009 0.3186 1
0.0086 0.2924 1
0.0104 1 0.5
0.0301 0.2636 0.1586
0.0047 0.287 0
0.0164 1 0.5
0.0094 0.3721 0
0.0027 1 0.5
0.1173 0.364 0.5992
0.0373 0.3357 0.4547
0.0163 0.4777 0.4633
0.0342 0.6751 0.6088
0.0337 0.3447 0.5
0.1159 0.4319 0.4068
0.0566 0.6369 1
0.0093 0.7123 0.5
0.0362618 0.796569 1
0.1153 0.4141 0.9229
0.0487 0.5238 1
0.0854 1 1
0.0116 0.2621 0.2545
0.0466 0.2667 0.2667
0.0648 1 1
0.0691 0.3175 1
0.0299 0.3068 1
0.0703 0.3444 0.5287
0.0076 0.4557 0.5
0.0054 1 0.5
0.0086 0.2924 1
0.0155 0.3735 0.5
0.1028 0.4176 1
0.0086 0.4536 1
0.0127 1 0.5
0.0284 0.3319 0.3292
0.1359 1 1
0.0899 0.3597 0.7084
0.0918 1 1
0.0362 0.2619 0.2575
0.189 0.4897 1
0.0416 0.4931 1
0.2154 0.3129 1
0.0043 1 0.5
0.1318 0.5074 0.5061
0.091 0.2553 0.5914
0.0366 0.3659 0.3635
0.0113 0.4176 1
0.014 0.2503 0.2503
0.0479 0.5542 0.5538
0.0357 1 1
0.0538 1 0.5
0.0645 0.5234 0.5721
0.0254 0.5067 1
0.0096 0.77 0
0.0102 0.2787 0.6918
0.0027 1 0.5
0.0104 1 0.5
0.0113 0.5467 1
0.0322 0.403 1
0.0241 1 1
0.0059 0.2606 0.8769
0.0646 1 1
0.0041 0.287 0
0.0205 0.2511 0.251
0.0027 1 0.5
0.0078 1 0.5
0.1217 0.4605 1
0.1278 0.2616 0.8983
0.0462 0.4399 0.819
0.0027 1 0.5
0.0086 0.2924 1
0.0081 1 0.5
0.0366 0.4236 1
0.05 0.5856 1
0.0113 0.4176 1
0.0285 0.2699 0.2696
0.0081 1 0.5
0.0373 0.3357 0.4547
0.0027 1 0.5
0.0271 0.3051 0.5
0.0834 0.4251 0.544
0.0054 1 0.5
0.0081 1 1
0.0548 0.6487 1
0.1375 0.8602 1
0.005 1 0.5
0.0343 0.3176 0.3176
0.0964 0.2532 0.2492
0.1907 1 1
0.0086 0.2924 1
0.0195 0.3731 1
0.0054 1 0.5
0.1451 0.2684 0.9684
0.0164 1 0.5
0.0027 1 0.5
0.0643 0.3659 1
0.0044 1 0.5
0.0353 1 0.5
0.1554 1 0.5
0.0104 0.2866 0.2124
0.0508 0.4139 1
0.0969 0.2549 0.4812
0.113631 1 1
0.0086 0.4154 1
0.0587 0.3132 0.1662
0.0917 1 0.5
0.1047 0.9665 0.5
0.1494 0.4277 0.4272
0.0081 1 0.5
0.0593 1 0.5
0.0279 0.2529 0.7602
0.1635 1 1
0.0142 1 0.5
0.0197 0.2525 0.2509
0.0027 1 0.5
0.0146 0.4002 0.3998
0.0148 0.3754 1
0.0081 1 0.5
0.1095 1 1
0.0054 1 0.5
0.0784 0.4986 1
0.0179 0.2531 0.2509
0.0112 0.562 1
0.005 0.2514 0.5
0.0183016 0.341261 0.793382
0.0086 0.2924 1
0.1751 1 1
0.0628 0.4845 1
0.0285 0.415 1
0.0373 0.3357 0.4547
0.0192 1 1
0.0524535 0.612595 1
0.0211 0.2503 0.2474
0.0289 0.3762 1
0.0205 0.2511 0.251
0.0441 1 0.5
0.0081 1 0.5
0.0054 1 0.5
0.0137 0.3518 0.5604
0.0044 0.2602 0
0.0027 1 0.5
0.0921 0.4705 0.7629
0.2228 0.4343 0.4398
0.2544 0.319 0
0.1507 0.2506 0.7398
0.0086 0.2924 1
0.0048 1 0.5
0.0442 0.2992 0.1592
0.0618 0.9523 1
0.1304 0.5229 1
0.1451 0.2684 0.9684
0.0492 0.5087 1
0.0411 0.5859 0
0.0398 0.473 1
0.1639 0.2939 0.7535
0.0154 1 0.5
0.0086 0.2924 1
0.2248 0.2846 0.2904
0.041 0.6817 0
0.0054 1 0.5
0.0047 0.287 0.2864
0.0049 1 0.5
0.0144 0.284 0.2732
0.0049 1 1
0.0491 0.5654 1
0.0373 0.3357 0.4547
0.0241 0.4892 1
0.5818 0.8498 0.8497
0.052 0.3087 1
0.0984 0.3944 1
0.1349 0.2681 1
0.0889 0.5911 1
0.121 0.4105 0.8627
0.0329 0.4058 1
0.0293955 1 0.5
0.0440984 1 1
0.0027 0.2673 1
0.0377 0.3328 0.3328
0.0054 1 0.5
0.0624 0.286 0.3868
0.0027 1 0.5
0.022 0.2555 0.2547
0.0171 0.715 1
0.041 0.257 0.2542
0.0182 0.5378 1
0.0086 0.392 1
0.0693 0.2643 0.7223
0.1502 0.3046 0.7237
0.1134 0.3006 1
0.1169 1 1
0.0168 0.4323 1
0.0366 0.6105 1
0.009 0.6548 1
0.0472 0.3112 1
0.0054 0.2774 0.1246
0.0325 0.2942 1
0.0077 0.3559 1
0.0416 0.254 0.2528
0.0293 0.4085 1
0.0839 1 1
0.0731 0.3838 1
0.0198 0.4154 1
0.0079 0.6471 0.5
0.0086 0.2924 1
0.0102 0.6495 0.5
0.0253 0.2997 1
0.1155 0.3295 0.4037
0.0636 0.4472 0.4472
0.0054 1 0.5
0.0373 0.3357 0.4547
0.0235 1 1
0.0027 1 0.5
0.1705 1 1
0.0882 0.5195 0.5195
0.1257 1 1
0.0054 1 0.5
0.0086 0.4289 1