Detailed view for LmjF.20.1180

Basic information

TDR Targets ID: 22430
Leishmania major, calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.0422 | Length (AA): 976 | MW (Da): 107643 | Paralog Number: 2

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00648   Calpain family cysteine protease
PF09149   Domain of unknown function (DUF1935)

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0004198   calcium-dependent cysteine-type endopeptidase activity  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
329 438 1r75 (A) 13 130 38.00 0 1 0.56 -1.17
451 976 1qxp (B) 20 601 22.00 0 1 0.47 0.81
461 787 1zcm (A) 39 351 29.00 0 1 0.56 -0.38
22 234 5cwi (A) 11 227 31.00 0.00012 1 0.484738 -0.24
37 193 5cwh (A) 2 158 10.00 0 0 0.316561 -1.09
318 441 2fe0 (A) 2 129 46.00 0 1 0.724549 -0.75
453 787 2p0r (A) 19 334 28.00 0 1 0.629938 -0.4
461 975 1qxp (B) 29 600 26.00 0 1 0.538364 1.16
462 788 2nqa (A) 30 342 29.00 0 1 0.623341 -0.25
465 787 1zcm (A) 43 351 32.00 0 1 0.682443 -0.29

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_135138)

Species Accession Gene Product
Leishmania braziliensis LbrM.20.5380   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania braziliensis LbrM.04.0490   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania donovani LdBPK_040430.1   calpain-like cysteine peptidase, putative
Leishmania infantum LinJ.20.1220   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania infantum LinJ.04.0430   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania infantum LinJ.20.1210   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania major LmjF.20.1185   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania major LmjF.20.1180   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania major LmjF.04.0450   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Leishmania mexicana LmxM.20.1185   calpain-like cysteine peptidase, putative
Leishmania mexicana LmxM.20.1180   calpain-like cysteine peptidase
Leishmania mexicana LmxM.04.0450   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Trypanosoma brucei gambiense Tbg972.9.4110   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Trypanosoma brucei gambiense Tbg972.1.1230   calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
Trypanosoma brucei Tb927.9.7540   cysteine peptidase, Clan CA, family C2, putative
Trypanosoma brucei Tb927.1.2100   Calpain-like protein 1.1
Trypanosoma congolense TcIL3000_0_54930   cysteine peptidase, Clan CA, family C2, putative
Trypanosoma cruzi TcCLB.503855.40   calpain-like cysteine peptidase, putative
Trypanosoma cruzi TcCLB.508999.190   cysteine peptidase, Clan CA, family C2, putative
Trypanosoma cruzi TcCLB.510957.9   calpain-like cysteine peptidase, putative
Trypanosoma cruzi TcCLB.510337.20   cysteine peptidase, Clan CA, family C2, putative

Essentiality

LmjF.20.1180 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.1.2100 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.1.2100 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.1.2100 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.1.2100 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb09.160.5550 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.160.5550 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.160.5550 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb09.160.5550 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania mexicana 337527   561727   585910  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 4174-73-6. chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in cell assay; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 7179420
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania mexicana 337527   561727   585910  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 4174-73-6. chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in cell assay; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 7179420
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from enzyme assay (ECO:0000005) Leishmania mexicana 337527   561727   585910  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 4174-73-6. chemical inhibition with known cysteine peptidase inhibitors leads to reduced enzyme activity in vitro; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 7179420
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania amazonensis No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: L-Amino acid esters appear to be hydrolyzed by cysteine peptidase and lead to slow growth of Leishmania sp. in cell assay; . References: 1428504 2235078 2345655
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania mexicana No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in cell assay; . References: 2270108
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from enzyme assay (ECO:0000005) Leishmania amazonensis 81384   576750  
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with known cysteine peptidase inhibitors leads to reduced enzyme activity in vitro; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 8023752
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from enzyme assay (ECO:0000005) Leishmania major 81384   576750  
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with known cysteine peptidase inhibitors leads to reduced enzyme activity in vitro; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 8023752
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) host (GO:0018995) Leishmania donovani No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 81989-95-9. chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in vivo (balb/c mouse) and cure of parasite burden; also provided protection against further infection (partial vaccine); General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 11238649
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania donovani No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 81989-95-9. chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in vivo (balb/c mouse) and cure of parasite burden; also provided protection against further infection (partial vaccine); General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 11238649
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) host (GO:0018995) Leishmania tropica No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 233277-99-1. chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in vivo (balb/c mouse lesions) and reduced lesion size; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 15172223
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) amastigote (BTO:0000062) inferred from bioassay (ECO:0000094) Leishmania tropica No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 233277-99-1. chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in vivo (balb/c mouse lesions) and reduced lesion size; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 15172223
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania tropica No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 233277-99-1. chemical inhibition with known cysteine peptidase inhibitors leads to slow growth of Leishmania sp. in cell assay; inhibitors overcome redundancy?; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 15172223
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania donovani 0   6693   81384   236103   327961   376038   543486   543487   575389   575390   575391   576750  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 22386-37-4; Drug: 36677-78-8; Drug: 52090-11-6; Drug: 98319-30-3; Drug: 300670-24-0; Drug: 302939-54-4; Drug: 303216-44-6; Drug: 309742-24-3; Drug: 312280-89-0; Drug: 315703-20-9; Drug: 320369-30-0; Drug: 322409-94-9; Drug: 330220-86-5; Drug: 335206-77-4; Drug: 347910-28-5; Drug: 352560-86-2; Drug: 363180-61-4; Drug: 413580-11-7; Drug: 416878-80-3; Drug: 419547-26-5; Drug: 428852-79-3; Drug: 825612-08-6; Drug: 825612-09-7; Drug: 825612-10-0. in silico virtual screening of falcipains lead to bioactive compounds against Leishmania promastigotes; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 15588096
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from in-silico analysis (ECO:0000043) Leishmania donovani 0   6693   81384   236103   327961   376038   543486   543487   575389   575390   575391   576750  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 22386-37-4; Drug: 36677-78-8; Drug: 52090-11-6; Drug: 98319-30-3; Drug: 300670-24-0; Drug: 302939-54-4; Drug: 303216-44-6; Drug: 309742-24-3; Drug: 312280-89-0; Drug: 315703-20-9; Drug: 320369-30-0; Drug: 322409-94-9; Drug: 330220-86-5; Drug: 335206-77-4; Drug: 347910-28-5; Drug: 352560-86-2; Drug: 363180-61-4; Drug: 413580-11-7; Drug: 416878-80-3; Drug: 419547-26-5; Drug: 428852-79-3; Drug: 825612-08-6; Drug: 825612-09-7; Drug: 825612-10-0. in silico virtual screening of falcipains lead to bioactive compounds against cysteine protease in vitro; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 15588096
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from specific protein inhibition (ECO:0000020) Leishmania donovani 0   6693   81384   236103   327961   376038   543486   543487   575389   575390   575391   576750  
Annotator: aaronjr@u.washington.edu. Comment: Drug: 22386-37-4; Drug: 36677-78-8; Drug: 52090-11-6; Drug: 98319-30-3; Drug: 300670-24-0; Drug: 302939-54-4; Drug: 303216-44-6; Drug: 309742-24-3; Drug: 312280-89-0; Drug: 315703-20-9; Drug: 320369-30-0; Drug: 322409-94-9; Drug: 330220-86-5; Drug: 335206-77-4; Drug: 347910-28-5; Drug: 352560-86-2; Drug: 363180-61-4; Drug: 413580-11-7; Drug: 416878-80-3; Drug: 419547-26-5; Drug: 428852-79-3; Drug: 825612-08-6; Drug: 825612-09-7; Drug: 825612-10-0. in silico virtual screening of falcipains lead to bioactive compounds against cysteine protease in vitro; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 15588096
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania donovani No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 2935-91-3; Drug: 24117-97-3; Drug: 98647-23-5; Drug: 198124-18-4; Drug: 256238-87-6; Drug: 257287-52-8; Drug: 263157-80-8; Drug: 294854-43-6; Drug: 303124-84-7; Drug: 303147-38-8; Drug: 321579-84-4; Drug: 331247-87-1; Drug: 337923-53-2; Drug: 338791-39-2; Drug: 675828-74-7; Drug: 676226-56-5; Drug: 678967-57-2; Drug: 681213-40-1; Drug: 686272-33-3; Drug: 696586-43-3; Drug: 705250-31-3; Drug: 708987-83-1; Drug: 831234-40-3; Drug: 882161-95-7; Drug: 882161-98-0; Drug: 882161-99-1; Drug: 882162-00-7; Drug: 882162-01-8; Drug: 882162-02-9; Drug: 882162-03-0; Drug: 882162-04-1; Drug: 882162-05-2; Drug: 882162-06-3; Drug: 882162-07-4; Drug: 882162-08-5; Drug: 882162-09-6; Drug: 882162-10-9; Drug: 882162-11-0; Drug: 882162-12-1; Drug: 882162-13-2; Drug: 882162-14-3. in silico virtual screening of falcipains lead to bioactive compounds against Leishmania promastigotes; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 16509575
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from in-silico analysis (ECO:0000043) Leishmania donovani No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 2935-91-3; Drug: 24117-97-3; Drug: 98647-23-5; Drug: 198124-18-4; Drug: 256238-87-6; Drug: 257287-52-8; Drug: 263157-80-8; Drug: 294854-43-6; Drug: 303124-84-7; Drug: 303147-38-8; Drug: 321579-84-4; Drug: 331247-87-1; Drug: 337923-53-2; Drug: 338791-39-2; Drug: 675828-74-7; Drug: 676226-56-5; Drug: 678967-57-2; Drug: 681213-40-1; Drug: 686272-33-3; Drug: 696586-43-3; Drug: 705250-31-3; Drug: 708987-83-1; Drug: 831234-40-3; Drug: 882161-95-7; Drug: 882161-98-0; Drug: 882161-99-1; Drug: 882162-00-7; Drug: 882162-01-8; Drug: 882162-02-9; Drug: 882162-03-0; Drug: 882162-04-1; Drug: 882162-05-2; Drug: 882162-06-3; Drug: 882162-07-4; Drug: 882162-08-5; Drug: 882162-09-6; Drug: 882162-10-9; Drug: 882162-11-0; Drug: 882162-12-1; Drug: 882162-13-2; Drug: 882162-14-3. in silico virtual screening of falcipains lead to bioactive compounds against cysteine protease in vitro; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 16509575
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from specific protein inhibition (ECO:0000020) Leishmania donovani No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 2935-91-3; Drug: 24117-97-3; Drug: 98647-23-5; Drug: 198124-18-4; Drug: 256238-87-6; Drug: 257287-52-8; Drug: 263157-80-8; Drug: 294854-43-6; Drug: 303124-84-7; Drug: 303147-38-8; Drug: 321579-84-4; Drug: 331247-87-1; Drug: 337923-53-2; Drug: 338791-39-2; Drug: 675828-74-7; Drug: 676226-56-5; Drug: 678967-57-2; Drug: 681213-40-1; Drug: 686272-33-3; Drug: 696586-43-3; Drug: 705250-31-3; Drug: 708987-83-1; Drug: 831234-40-3; Drug: 882161-95-7; Drug: 882161-98-0; Drug: 882161-99-1; Drug: 882162-00-7; Drug: 882162-01-8; Drug: 882162-02-9; Drug: 882162-03-0; Drug: 882162-04-1; Drug: 882162-05-2; Drug: 882162-06-3; Drug: 882162-07-4; Drug: 882162-08-5; Drug: 882162-09-6; Drug: 882162-10-9; Drug: 882162-11-0; Drug: 882162-12-1; Drug: 882162-13-2; Drug: 882162-14-3. in silico virtual screening of falcipains lead to bioactive compounds against cysteine protease in vitro; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 16509575
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania major No drug identifiers listed for this gene.
Annotator: aaronjr@u.washington.edu. Comment: Drug: 872361-01-8; Drug: 872361-02-9; Drug: 886061-72-9; Drug: 886061-73-0; Drug: 886061-74-1; Drug: 886061-75-2; Drug: 886061-76-3; Drug: 886061-77-4; Drug: 886061-78-5; Drug: 886061-79-6; Drug: 886061-80-9; Drug: 886061-81-0; Drug: 886061-82-1; Drug: 886061-85-4; Drug: 886061-86-5; Drug: 886061-87-6; Drug: 886061-88-7; Drug: 886061-89-8; Drug: 886061-90-1; Drug: 886061-91-2; Drug: 886061-92-3; Drug: 886061-93-4; Drug: 886061-99-0; Drug: 886062-00-6; Drug: 886062-05-1; Drug: 886062-06-2; Drug: 886062-07-3; Drug: 886062-08-4; Drug: 886062-09-5; Drug: 886062-10-8; Drug: 886062-11-9; Drug: 886062-12-0; Drug: 886062-13-1; Drug: 886062-14-2; Drug: 886062-15-3; Drug: 886062-16-4; Drug: 886062-17-5; Drug: 906674-95-1. chemical inhibition with peptidomimetics and Aziridine-2,3-dicarboxylates leads to slow growth of Leishmania sp. in cell assay; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 16801424
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) promastigote (BTO:0001124) inferred from bioassay (ECO:0000094) Leishmania amazonensis 30267  
Annotator: aaronjr@u.washington.edu. Comment: chemical inhibition with calpain inhibitor leads to slow growth of Leishmania sp. in cell assay; General cysteine peptidase inhibition with anti-leishmanial activity; specific target unknown. References: 16842979

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

  • Validation: inhibition during in vitro culture
  • annotated by: aaronjr@u.washington.edu.
  • References: 16842979
  • Validation: in vivo inhibition
  • annotated by: aaronjr@u.washington.edu.
  • References: 11238649 15172223

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

Compound Source Reference
Curated by TDRTargets References
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Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative Compounds References
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative 754 aa 25.1% 720 aa Compounds References
Leishmania major cytoskeleton-associated protein CAP5.5, putative,cysteine peptidase, Clan CA, family C2, putative 723 aa 35.5% 662 aa Compounds References
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative 414 aa 27.9% 412 aa Compounds References
Leishmania major small myristoylated protein-3, putative 115 aa 42.1% 107 aa Compounds References
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative 855 aa 33.4% 707 aa Compounds References
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative 687 aa 38.9% 669 aa Compounds References
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative 732 aa 35.0% 679 aa Compounds References
Leishmania major small myristoylated protein-2 136 aa 40.5% 116 aa Compounds References
Leishmania major calpain family cysteine protease-like protein 705 aa 33.6% 655 aa Compounds References
Leishmania major small myristoylated protein-1 131 aa 47.1% 123 aa Compounds References
Leishmania major calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative 743 aa 35.5% 708 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0912 0.3593 1
0.0846 0.3022 0.6732
0.0772 0.6292 0.6882
0.0836 0.4265 0.6912
0.1045 0.2596 0.5082
0.0874 0.975 1
0.0785 0.4911 1
0.1059 0.2888 0.6328
0.082 0.2815 0.6293
0.0897 0.9941 1
0.0995 0.2618 0.4943
0.1439 0.7286 1
0.0537 0.623 1
0.0874 0.4365 0.7757
0.1045 0.2596 0.5082
0.0904 0.307 0.6729
0.0837 0.7377 1
0.0884 0.5444 1
0.0804 0.323 0.8502
0.0866 0.8734 1
0.0811 0.8272 1
0.1439 0.7286 1
0.0862 0.3187 1
0.0803 0.575 1
0.0995 0.2618 0.4943
0.100192 1 1
0.0945 0.4992 1
0.0802 0.7948 1
0.0878 0.2535 1
0.0826 0.8051 1
0.0734 0.2647 1
0.0772 0.6292 0.6882
0.0789 0.7423 1
0.0868 0.6975 1
0.1891 0.7276 1
0.0896 0.3841 0.7974
0.0924 0.5378 0.8968
0.0857 0.2889 0.6068
0.0752 0.8206 0.8206
0.0732 0.8122 1
0.081 0.592 0.939
0.0883 0.9132 1
0.1059 0.2888 0.6328
0.0779 0.9333 1
0.1891 0.7283 1
0.099 0.2885 0.6193
0.164447 1 1
0.0876 0.9978 1
0.0776 0.8501 1
0.0803 0.4345 1
0.092 0.2968 0.6396
0.0699 0.3804 1
0.0537 0.6482 1
0.0774 0.2962 0.6202
0.0848 0.887 1
0.0982 0.2596 0.5487
0.039 0.2582 1
0.0958 0.7454 1
0.0878 0.8921 1
0.0497 0.4878 0.4853
0.0819 0.3347 0.9363
0.0884 0.5478 1
0.0974 0.2547 0.5053
0.0818 0.3004 0.8989
0.0797 0.9228 1
0.031 0.3303 1
0.0919 0.3462 1
0.0884 0.3557 0.6749
0.1109 0.2882 0.6779
0.1891 0.7276 1
0.1086 0.828 1
0.0497 0.4878 0.4853
0.0848 0.6006 1
0.0974 0.2547 0.5053
0.1538 0.7298 1
0.0868 0.6975 1
0.0847 0.4873 1
0.0901 0.4183 0.7841
0.0903 0.921 1
0.0848 0.8957 1
0.1042 0.3194 0.7003
0.0912 0.376 0.7164
0.0862 0.3249 1
0.0803 0.2876 1
0.0884 0.5444 1
0.0912 0.3281 1
0.0892 0.6143 1
0.0349 0.4233 1
0.0772 0.6292 0.6882
0.0931 0.6669 0.9959
0.0982 0.2596 0.5487
0.0901 0.4183 0.7841
0.0497 0.4878 0.4853
0.1045 0.2596 0.5082
0.0982 0.2596 0.5487
0.0871 0.3076 0.6455
0.0792 0.8844 1
0.0603 0.4489 1
0.089 0.3254 0.8047
0.0907 0.3426 0.8959
0.0811 0.2665 1
0.1045 0.2596 0.5082
0.085 0.4543 0.7716
0.0974 0.2547 0.5053
0.0865 0.3964 0.9157
0.1044 0.3277 0.7099
0.0772 0.7608 1
0.0958 0.9998 1
0.071 0.5552 1
0.0995 0.2618 0.4943
0.0887 0.9024 1
0.0991 0.6169 1
0.0942 0.7508 1
0.0811 0.2665 1
0.091 0.4857 1
0.0842 0.91 1
0.0792 0.8844 1
0.0766 0.7977 1
0.0765 0.7745 0.7738
0.0878 0.2535 1
0.1608 0.7283 1
0.0972 0.4318 0.8933
0.0797 0.5209 0.9221
0.0772 0.7613 1
0.0926 0.2807 0.516
0.0822 0.5335 0.9273
0.0875 0.4445 0.8188
0.0766 0.7977 1
0.0812 0.6045 0.9492
0.0497 0.4878 0.4853
0.0842 0.91 1
0.089 0.2838 0.5085
0.0679 0.4738 1
0.0852 0.5692 1
0.0815 0.4807 1
0.0934 0.9636 1
0.0831 0.5389 0.902
0.0911 0.4582 0.891
0.0907 0.2907 0.5128
0.0829 0.328 0.7039
0.0969 0.5878 1
0.039 0.3921 1
0.09 0.4109 0.6608
0.0789 0.7423 1
0.1792 0.7283 1
0.0995 0.2618 0.4943
0.0803 0.9137 1
0.0799 0.2919 1
0.0949 0.3805 0.7494
0.0811 0.2665 1
0.0835 0.2598 1
0.0765 0.7745 0.7738
0.1496 0.3111 1
0.091 0.4857 1
0.1792 0.7283 1
0.087 0.2905 0.5821
0.1891 0.7276 1
0.0934 0.9798 1
0.0884 0.3557 0.6749
0.0985 0.6125 1
0.0776 0.8501 1
0.0435 0.4305 1
0.0884 0.3557 0.6749
0.0857 0.2889 0.6068
0.164447 1 1
0.0408 0.2712 1
0.1059 0.2888 0.6328
0.0982 0.2596 0.5487
0.0497 0.4878 0.4853
0.0927 0.3963 1
0.0974 0.2547 0.5053
0.0974 0.2547 0.5053
0.0863 0.2735 1
0.0584 0.3017 0.547
0.0868 0.6975 1
0.0803 0.5392 1
0.0934 0.9798 1
0.1891 0.7283 1
0.0924 0.5378 0.8968
0.193072 1 1
0.0789 0.7423 1
0.0819 0.8779 1
0.0763 0.2733 0.669
0.0995 0.4621 1
0.0839 0.4446 0.9245
0.0848 0.4027 1
0.0848 0.6006 1
0.0803 0.2876 1
0.08 0.3162 0.9311
0.093 0.3138 0.656
0.0772 0.7608 1
0.0942 0.5998 1
0.1203 0.2771 0.616
0.0804 0.323 0.8502
0.0848 0.3166 0.892
0.0912 0.3254 1
0.0763 0.2733 0.669
0.0907 0.3426 0.8959
0.0818 0.3004 0.8989
0.0835 0.7054 1
0.0803 0.5392 1
0.0887 0.3574 0.635
0.0916 0.3025 0.6899
0.0995 0.2618 0.4943
0.0912 0.3281 1
0.0852 0.5692 1
0.0843 0.5787 1
0.0803 0.9137 1
0.0848 0.4027 1
0.0883 0.9132 1
0.0912 0.2962 0.5683
0.0868 0.6975 1
0.0491 0.2608 0.7464
0.1013 0.3183 0.6894
0.0803 0.9137 1
0.0848 0.6006 1
0.0995 0.2618 0.4943
0.0849 0.4343 0.9495
0.1792 0.7283 1
0.0862 0.4806 0.889
0.0772 0.6292 0.6882
0.0912 0.3593 1
0.0761 0.6275 1
0.0829 0.5956 0.945
0.071 0.5552 1
0.0884 0.4473 0.8471
0.0903 0.9937 1
0.0902 0.3687 0.6205
0.1891 0.7276 1
0.093 0.4006 0.7661
0.0497 0.4878 0.4853
0.0819 0.3347 0.9363
0.0584 0.3017 0.547
0.0862 0.3249 1
0.0815 0.4807 1
0.0497 0.4878 0.4853
0.0811 0.8272 1
0.0848 0.6006 1
0.0912 0.3281 1
0.0825 0.5382 0.9005
0.1538 0.7298 1
0.0811 0.2665 1
0.0497 0.4878 0.4853
0.0848 0.8957 1
0.075 0.6083 1
0.0772 0.7603 0.9861
0.0848 0.8957 1
0.0785 0.8806 1
0.0863 0.6087 0.8455
0.0788 0.5961 1
0.0864 0.9524 1
0.0928 0.3007 0.5278
0.0931 0.6669 0.9959
0.0884 0.3526 1
0.0912 0.3281 1
0.091 0.4857 1
0.1439 0.7251 1
0.0845 0.6701 1
0.0825 0.3547 1
0.093 0.4006 0.7661
0.0917 0.2984 0.6339
0.0995 0.4621 1
0.0855 0.4951 1
0.0837 0.7377 1
0.0808 0.4458 1
0.1045 0.2596 0.5082
0.093 0.4006 0.7661
0.0803 0.4345 1
0.0942 0.3697 1
0.0764 0.8752 1
0.0984 0.3305 0.7298
0.0927 0.2694 1
0.0772 0.6292 0.6882
0.0935 0.9848 1
0.093 0.4006 0.7661
0.0844 0.3408 0.9264
0.0803 0.575 1
0.0927 0.2694 1
0.0803 0.751 1
0.0942 0.7508 1
0.0763 0.2733 0.669
0.078 0.7592 1
0.0961991 0.711247 1
0.092 0.2968 0.6396
0.0876 0.6852 1

Assayability

Assay information

  • Assay for Cathepsin B (3.4.22.1 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

30 literature references were collected for this gene.

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Gene identifier LmjF.20.1180 (Leishmania major), calpain-like cysteine peptidase, putative,cysteine peptidase, Clan CA, family C2, putative
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