pI: 5.2775 |
Length (AA): 373 |
MW (Da): 40554 |
Paralog Number:
0
Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0
Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable
Modbase 3D models:
There are 6 models calculated for this protein. More info on
these models, including the
models themselves is available at:
Modbase
Target Beg | Target End | Template | Template Beg | Template End | Identity | Evalue | Model Score | MPQS | zDope |
---|---|---|---|---|---|---|---|---|---|
12 | 368 | 1j8f (A) | 57 | 356 | 42.00 | 0 | 1 | 1.17 | -0.21 |
14 | 273 | 1m2k (A) | 1 | 245 | 27.00 | 0 | 1 | 1.05 | -0.83 |
5 | 279 | 5btr (A) | 229 | 504 | 37.00 | 0 | 1 | 1.19697 | -0.35 |
8 | 361 | 4x3o (A) | 53 | 350 | 43.00 | 0 | 1 | 1.24976 | 0.04 |
15 | 262 | 4bn4 (A) | 121 | 365 | 44.00 | 0 | 1 | 1.21238 | -0.33 |
18 | 262 | 4v1c (K) | 124 | 365 | 47.00 | 0.0000017 | 1 | 0.534536 | 1.67 |
Help me make sense of these data.
A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.
PDB Structures:
Upregulation Percent | Ranking | Stage | Dataset |
---|---|---|---|
Upper 60-80% percentile | amastigotes. | Fernandes MC |
Upregulation Percent | Ranking | Stage | Dataset |
---|---|---|---|
Mid 40-60% percentile | metacyclic. | Fernandes MC |
Fernandes MC | Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures. |
Ortholog group members (OG5_127826)
Species | Accession | Gene Product |
---|---|---|
Brugia malayi | Bm1_30475 | transcriptional regulator, Sir2 family protein |
Candida albicans | CaO19.10112 | likely SIR2 family histone deacetylase similar to S. cerevisiae HST2 (YPL015C) involved in telomeric chromatin silencing |
Candida albicans | CaO19.2580 | likely SIR2 family histone deacetylase similar to S. cerevisiae HST2 (YPL015C) involved in telomeric chromatin silencing |
Dictyostelium discoideum | DDB_G0283917 | NAD(+)-dependent deacetylase, silent information regulator protein family protein |
Drosophila melanogaster | Dmel_CG5085 | CG5085 gene product from transcript CG5085-RB |
Echinococcus granulosus | EgrG_001176600 | NAD dependent deacetylase sirtuin 3 |
Echinococcus granulosus | EgrG_001065100 | chromatin regulatory protein sir2 |
Entamoeba histolytica | EHI_151300 | Sir2 family transcriptional regulator, putative |
Entamoeba histolytica | EHI_201210 | Sir2 family transcriptional regulator, putative |
Echinococcus multilocularis | EmuJ_001065100 | chromatin regulatory protein sir2 |
Echinococcus multilocularis | EmuJ_001176600 | NAD dependent deacetylase sirtuin 3 |
Giardia lamblia | GL50803_10708 | Hypothetical protein |
Homo sapiens | ENSG00000142082 | sirtuin 3 |
Homo sapiens | ENSG00000068903 | sirtuin 2 |
Leishmania braziliensis | LbrM.26.0210a | NAD-dependent SIR2, putative |
Leishmania donovani | LdBPK_260200.1 | Silent information regulator 2 related protein 1 |
Leishmania infantum | LinJ.26.0200 | silent information regulator 2, putative |
Leishmania major | LmjF.26.0210 | silent information regulator 2, putative |
Leishmania mexicana | LmxM.26.0210 | silent information regulator 2, putative,NAD-dependent SIR2, putative |
Loa Loa (eye worm) | LOAG_05338 | transcriptional regulator |
Mus musculus | ENSMUSG00000015149 | sirtuin 2 |
Mus musculus | ENSMUSG00000025486 | sirtuin 3 |
Saccharomyces cerevisiae | YPL015C | histone deacetylase HST2 |
Schistosoma japonicum | Sjp_0303650 | ko:K01463 sirtuin (silent mating type information regulation 2 homolog) 2 [EC:3.5.1.-], putative |
Schistosoma mansoni | Smp_084140.3 | chromatin regulatory protein sir2 |
Schistosoma mansoni | Smp_084140.4 | chromatin regulatory protein sir2 |
Schistosoma mansoni | Smp_084140.1 | chromatin regulatory protein sir2 |
Schmidtea mediterranea | mk4.002285.00 | NAD-dependent protein deacetylase sirtuin-3, mitochondrial |
Schmidtea mediterranea | mk4.013539.01 | NAD-dependent protein deacetylase sirtuin-3, mitochondrial |
Schmidtea mediterranea | mk4.014730.01 | NAD-dependent protein deacetylase sirtuin-3, mitochondrial |
Trypanosoma brucei gambiense | Tbg972.7.1700 | silent information regulator 2,NAD-dependent SIR2 |
Trypanosoma brucei | Tb927.7.1690 | Silent information regulator 2 related protein 1 |
Trypanosoma congolense | TcIL3000_0_37270 | silent information regulator 2 |
Trypanosoma cruzi | TcCLB.508207.150 | Silent information regulator 2 related protein 1 |
Trypanosoma cruzi | TcCLB.507519.60 | Silent information regulator 2 related protein 1 |
Trichomonas vaginalis | TVAG_362260 | chromatin regulatory protein sir2, putative |
Trichomonas vaginalis | TVAG_413390 | chromatin regulatory protein sir2, putative |
Gene/Ortholog | Organism | Phenotype | Source Study |
---|---|---|---|
Tb927.7.1690 | Trypanosoma brucei | significant gain of fitness in bloodstream forms (3 days) | alsford |
Tb927.7.1690 | Trypanosoma brucei | significant gain of fitness in bloodstream forms (6 days) | alsford |
Tb927.7.1690 | Trypanosoma brucei | significant gain of fitness in procyclic forms | alsford |
Tb927.7.1690 | Trypanosoma brucei | significant gain of fitness in differentiation of procyclic to bloodstream forms | alsford |
gerdes | Experimental determination and system-level analysis of essential genes in E. coli MG1655 | Gerdes et al., J Bacteriol. 2003 185:5673-84 |
keio | Systematic single-gene knock-out mutants of E. coli K12 | The Keio Collection |
nmpdr | Genome-scale essentiality datasets from published studies (M. tuberculosis) | National Microbial Pathogen Data Resource |
wormbase | C. elegans RNAi experiments | WormBase web site, http://www.wormbase.org, release WS170 |
alsford | High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome | Genome Res 2011, 21:915-924 |
yeastgenome | Systematic deletion of yeast genes | Saccharomyces Genome Database |
blattner | Systematic mutagenesis of the E. coli (MG1655) genome | J Bacteriol 2004, 186:4921-4930 |
neb | C. elegans RNAi phenotypes | Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs |
shigen | Profiling of E. coli Chromosome (PEC) | National Institute of Genetics, Japan |
Affected Entity | Phenotypic quality | Occurs in | Occurs at | Evidence | Observed in | Drugs/Inhibitors |
---|---|---|---|---|---|---|
growth (GO:0040007) | decreased time (PATO:0000716) | single cell organism (CARO:0000064) | amastigote (BTO:0000062) | inferred from specific protein inhibition (ECO:0000020) | Leishmania infantum | No drug identifiers listed for this gene. |
Annotator: | aaronjr@u.washington.edu. | Comment: | Drug: 410536-97-9. chemical inhibition with SIR2 inhibitor leads to slow growth of Leishmania sp. in cell assay; . | References: | 15860278 | |
growth (GO:0040007) | decreased time (PATO:0000716) | single cell organism (CARO:0000064) | amastigote (BTO:0000062) | inferred from bioassay (ECO:0000094) | Leishmania infantum | No drug identifiers listed for this gene. |
Annotator: | aaronjr@u.washington.edu. | Comment: | Drug: 410536-97-9. chemical inhibition with SIR2 inhibitor leads to slow growth of Leishmania sp. in cell assay; . | References: | 15860278 | |
DNA fragmentation during apoptosis (GO:0006309) | active (PATO:0001707) | single cell organism (CARO:0000064) | amastigote (BTO:0000062) | inferred from visible phenotype (ECO:0000176) | Leishmania infantum | No drug identifiers listed for this gene. |
Annotator: | aaronjr@u.washington.edu. | Comment: | Drug: 410536-97-9. chemical inhibition with SIR2 inhibitor causes apoptotic like cell-death and DNA fragmentation; . | References: | 15860278 | |
growth (GO:0040007) | drug insensitive (PATO:0000528) | single cell organism (CARO:0000064) | promastigote (BTO:0001124) | inferred from specific protein inhibition (ECO:0000020) | Leishmania infantum | No drug identifiers listed for this gene. |
Annotator: | aaronjr@u.washington.edu. | Comment: | Drug: 410536-97-9. overexpression of recombinant enzyme confers resistance to SIR2 inhibitor; . | References: | 15860278 | |
growth (GO:0040007) | drug insensitive (PATO:0000528) | single cell organism (CARO:0000064) | promastigote (BTO:0001124) | inferred from bioassay (ECO:0000094) | Leishmania infantum | No drug identifiers listed for this gene. |
Annotator: | aaronjr@u.washington.edu. | Comment: | Drug: 410536-97-9. overexpression of recombinant enzyme confers resistance to SIR2 inhibitor; . | References: | 15860278 | |
syncytium formation by plasma membrane fusion (GO:0000768) | biosynthetic process (GO:0009058) | single cell organism (CARO:0000064) | amastigote (BTO:0000062) | inferred from loss-of-function mutant phenotype (ECO:0000016) | Leishmania infantum | No drug identifiers listed for this gene. |
Annotator: | aaronjr@u.washington.edu. | Comment: | Genetic disruption of the SIR2 gene reduces ability of amastigotes to survive intracellularly; targeted double KO cannot be generated unless an episome is present that expresses a functional copy; . | References: | 16236469 | |
viability (PATO:0000169) | decreased (PATO:0000468) | single cell organism (CARO:0000064) | amastigote (BTO:0000062) | inferred from loss-of-function mutant phenotype (ECO:0000016) | Leishmania infantum | No drug identifiers listed for this gene. |
Annotator: | aaronjr@u.washington.edu. | Comment: | Genetic disruption of the SIR2 gene reduces ability of amastigotes to survive intracellularly; targeted double KO cannot be generated unless an episome is present that expresses a functional copy; . | References: | 16236469 |
In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
In any case, if you have information about papers containing relevant validation data for this target, please contact us.
Druggability index (range: 0 to 1): 0.2
Species | Known druggable target | Linked compounds | Reference |
---|---|---|---|
Homo sapiens | sirtuin 2 | Compounds | References |
Leishmania infantum | silent information regulator 2, putative | Compounds | References |
Homo sapiens | sirtuin 3 | Compounds | References |
Saccharomyces cerevisiae | histone deacetylase HST2 | Compounds | References |
12 literature references were collected for this gene.