Detailed view for LmjF.36.4100

Basic information

TDR Targets ID: 22568
Leishmania major, glycosyl hydrolase-like protein

Source Database / ID:  TriTrypDB  GeneDB

pI: 7.1683 | Length (AA): 1469 | MW (Da): 157765 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01055   Glycosyl hydrolases family 31

Gene Ontology

Mouse over links to read term descriptions.
GO:0004553   hydrolase activity, hydrolyzing O-glycosyl compounds  
GO:0005975   carbohydrate metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
206 1205 2f2h (A) 2 769 16.00 0 1 0.5 0.11
352 1255 2g3m (A) 3 693 18.00 0 1 0.45 0.07
544 791 1uas (A) 1 246 11.00 0 0.05 0.22 -0.21
235 1301 3l4y (A) 7 848 17.00 0 1 0.568744 0.98
530 1071 5jou (A) 377 819 25.00 0.0046 0.96 0.381658 0.68
917 1017 3loo (A) 74 179 33.00 0.28 0.15 0.172454 1.86
1319 1440 2jda (A) 7 133 11.00 0.00029 0.04 0.0844497 0.43
1320 1441 4zy9 (A) 1 129 15.00 0.0017 0.07 0.0924497 -0.02

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_154734)

Species Accession Gene Product
Leishmania braziliensis LbrM.35.4340   glycosyl hydrolase-like protein
Leishmania donovani LdBPK_364300.1   glycosyl hydrolase-like protein
Leishmania infantum LinJ.36.4300   glycosyl hydrolase-like protein
Leishmania major LmjF.36.4100   glycosyl hydrolase-like protein
Leishmania mexicana LmxM.36.4100   glycosyl hydrolase-like protein
Trypanosoma brucei gambiense Tbg972.11.11630   glycosyl hydrolase-like protein, putative
Trypanosoma brucei Tb927.11.10370   glycosyl hydrolase-like protein
Trypanosoma cruzi TcCLB.510283.70   glycosyl hydrolase-like protein, putative
Trypanosoma cruzi TcCLB.503823.40   glycosyl hydrolase-like protein, putative

Essentiality

LmjF.36.4100 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.2140 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.2140 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.2140 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.2140 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.4


Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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