Detailed view for LmjF.32.1650

Basic information

TDR Targets ID: 22603
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.3643 | Length (AA): 923 | MW (Da): 99321 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
GO:0019888   protein phosphatase regulator activity  
GO:0000159   protein phosphatase type 2A complex  
GO:0008601   protein phosphatase type 2A regulator activity  
GO:0005515   protein binding  
GO:0007165   signal transduction  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
470 822 1erj (A) 287 675 14.00 0 0.73 0.23 0.22
243 349 2mqa (A) 30 136 13.00 0 0.01 0.290726 -0.74
521 852 3dw8 (B) 87 424 29.00 0 1 0.487797 1.1
641 717 4elm (H) 109 211 36.00 0.87 0.13 0.131524 0.93
873 923 4wjs (A) 174 223 20.00 0.26 0.41 0.238355 -0.07

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_126981)

Species Accession Gene Product
Arabidopsis thaliana AT1G51690   serine/threonine protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform
Arabidopsis thaliana AT1G17720   serine/threonine protein phosphatase 2A 55 kDa regulatory subunit B beta isoform
Brugia malayi Bm1_23460   serine/threonine protein phosphatase 2A
Candida albicans CaO19.1468   subunit of protein phosphatase 2A
Candida albicans CaO19.9043   subunit of protein phosphatase 2A
Caenorhabditis elegans CELE_F26E4.1   Protein SUR-6
Dictyostelium discoideum DDB_G0283905   protein phosphatase 2A B55 regulatory subunit
Drosophila melanogaster Dmel_CG6235   twins
Echinococcus granulosus EgrG_001000500   protein phosphatase pp2a regulatory subunit b
Entamoeba histolytica EHI_148170   protein phosphatase regulatory subunit, putative
Echinococcus multilocularis EmuJ_001000500   protein phosphatase pp2a regulatory subunit b
Giardia lamblia GL50803_4079   Protein phosphatase PP2A regulatory subunit B
Homo sapiens ENSG00000221914   protein phosphatase 2, regulatory subunit B, alpha
Homo sapiens ENSG00000074211   protein phosphatase 2, regulatory subunit B, gamma
Homo sapiens 55844   protein phosphatase 2, regulatory subunit B, delta
Homo sapiens ENSG00000156475   protein phosphatase 2, regulatory subunit B, beta
Leishmania braziliensis LbrM.33.0710   protein phosphatase 2A regulatory subunit, putative
Leishmania braziliensis LbrM.32.1820   hypothetical protein, conserved
Leishmania donovani LdBPK_330720.1   protein phosphatase 2A regulatory subunit, putative
Leishmania donovani LdBPK_321730.1   hypothetical protein, conserved
Leishmania infantum LinJ.32.1730   hypothetical protein, conserved
Leishmania infantum LinJ.33.0720   protein phosphatase 2A regulatory subunit, putative
Leishmania major LmjF.33.0670   protein phosphatase 2A regulatory subunit, putative
Leishmania major LmjF.32.1650   hypothetical protein, conserved
Leishmania mexicana LmxM.31.1650   hypothetical protein, conserved
Leishmania mexicana LmxM.32.0670   protein phosphatase 2A regulatory subunit, putative
Loa Loa (eye worm) LOAG_09069   serine/threonine protein phosphatase 2A
Mus musculus ENSMUSG00000041769   protein phosphatase 2, regulatory subunit B, delta
Mus musculus ENSMUSG00000022052   protein phosphatase 2, regulatory subunit B, alpha
Mus musculus ENSMUSG00000029120   protein phosphatase 2, regulatory subunit B, gamma
Mus musculus ENSMUSG00000024500   protein phosphatase 2, regulatory subunit B, beta
Oryza sativa 4328769   Os02g0224200
Oryza sativa 9266142   Os02g0617600
Oryza sativa 4341313   Os06g0563300
Oryza sativa 9270873   Os01g0600200
Onchocerca volvulus OVOC11899   Protein phosphatase PP2A 55 kDa regulatory subunit homolog
Saccharomyces cerevisiae YGL190C   protein phosphatase 2A regulatory subunit CDC55
Schistosoma japonicum Sjp_0110960   similar to Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform, putative
Schistosoma mansoni Smp_173810   protein phosphatase pp2a regulatory subunit B
Schmidtea mediterranea mk4.001383.03  
Schmidtea mediterranea mk4.001176.04   Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B
Schmidtea mediterranea mk4.003295.00   Protein phosphatase PP2A 55 kDa regulatory subunit
Schmidtea mediterranea mk4.003732.02  
Trypanosoma brucei gambiense Tbg972.11.16660   hypothetical protein, conserved
Trypanosoma brucei gambiense Tbg972.10.13720   protein phosphatase 2A regulatory subunit, putative
Trypanosoma brucei Tb927.10.11360   protein phosphatase 2A regulatory subunit, putative
Trypanosoma brucei Tb927.11.14850   hypothetical protein, conserved
Trypanosoma congolense TcIL3000.11.15140   hypothetical protein, conserved
Trypanosoma congolense TcIL3000_10_9590   protein phosphatase 2A regulatory subunit, putative
Trypanosoma cruzi TcCLB.507709.100   protein phosphatase 2A regulatory subunit, putative
Trypanosoma cruzi TcCLB.506221.100   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.511717.20   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.506577.130   protein phosphatase 2A regulatory subunit, putative
Trichomonas vaginalis TVAG_402640   protein phosphatase pp2a regulatory subunit B, putative
Trichomonas vaginalis TVAG_481490   protein phosphatase PP2A regulatory subunit B, putative
Trichomonas vaginalis TVAG_081750   serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B gamma isoform, putative

Essentiality

LmjF.32.1650 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.6480 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.6480 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.6480 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb11.01.6480 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.11360 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.11360 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.11360 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.11360 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F26E4.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_F26E4.1 Caenorhabditis elegans larval lethal wormbase
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Gallus gallus Serine-threonine protein phosphatase 2A regulatory subunit Compounds References
Homo sapiens protein phosphatase 2, regulatory subunit B, alpha Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0595 0.5545 1
0.006 1 1
0.0489 0.618 1
0.037 0.6175 1
0.0074 0.5177 1
0.0397 0.6166 1
0.049 0.4584 1
0.0683 0.4519 1
0.0074 1 1
0.0597 0.5534 1
0.0051 0.4323 0.5
0.0212 0.2522 1
0.0244 1 1
0.0517 0.5226 1
0.0644 0.5637 1
0.0683 0.4519 1
0.049 0.4584 1
0.0135 0.3368 1
0.0054 0.3837 0.5
0.0341 0.2783 1
0.0212 0.5215 1
0.0385 0.344 1
0.0607 0.539 1
0.0282 0.639 0.5
0.0341 0.4298 1
0.0147 0.3368 1
0.0449 0.3991 1
0.0588 0.4872 1
0.0572 0.7155 1
0.0047 0.6842 1
0.041 0.6166 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier LmjF.32.1650 (Leishmania major), hypothetical protein, conserved
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