Detailed view for LmjF.25.0120

Basic information

TDR Targets ID: 22825
Leishmania major, electron transfer flavoprotein, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.0965 | Length (AA): 258 | MW (Da): 28240 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01012   Electron transfer flavoprotein domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0009055   electron carrier activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
9 254 1efv (B) 4 251 49.00 0 1 1.57 -0.81
9 252 1efp (B) 1 246 54.00 0 1 1.66 -0.89
8 253 2a1t (S) 3 250 48.00 0 1 1.51519 -0.28
9 252 1efp (B) 1 246 54.00 0 1 1.62264 -0.47

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127708)

Species Accession Gene Product
Arabidopsis thaliana AT5G43430   electron transfer flavoprotein subunit beta
Brugia malayi Bm1_43265   Electron transfer flavoprotein beta-subunit
Candida albicans CaO19.6612   electron-transferring flavoprotein
Caenorhabditis elegans CELE_F23C8.5   Protein F23C8.5
Dictyostelium discoideum DDB_G0277991   electron transfer flavoprotein beta subunit
Drosophila melanogaster Dmel_CG7834   CG7834 gene product from transcript CG7834-RB
Escherichia coli b0041   anaerobic carnitine reduction putative electron transfer flavoprotein subunit
Escherichia coli b1697   putative electron transfer flavoprotein subunit
Homo sapiens ENSG00000105379   electron-transfer-flavoprotein, beta polypeptide
Leishmania braziliensis LbrM.25.0120   electron transfer flavoprotein, putative
Leishmania donovani LdBPK_250120.1   electron transfer flavoprotein, putative
Leishmania infantum LinJ.25.0120   electron transfer flavoprotein, putative
Leishmania major LmjF.25.0120   electron transfer flavoprotein, putative
Leishmania mexicana LmxM.25.0120   electron transfer flavoprotein, putative
Loa Loa (eye worm) LOAG_11220   electron transfer flavoprotein beta-subunit
Mycobacterium leprae ML1712c   PROBABLE ELECTRON TRANSFER FLAVOPROTEIN (BETA-SUBUNIT) FIXA (BETA-ETF) (ELECTRON TRANSFER FLAVOPROTEIN SMALL SUBUNIT) (ETFSS)
Mus musculus ENSMUSG00000004610   electron transferring flavoprotein, beta polypeptide
Mus musculus 102642019   electron transfer flavoprotein subunit beta-like
Mycobacterium tuberculosis Rv3029c   Probable electron transfer flavoprotein (beta-subunit) FixA (beta-ETF) (electron transfer flavoprotein small subunit) (ETFSS)
Mycobacterium ulcerans MUL_1922   electron transfer flavoprotein FixA
Neospora caninum NCLIV_038520   Electron transfer flavoprotein domain, related
Oryza sativa 4335102   Os04g0182800
Onchocerca volvulus OVOC10506  
Saccharomyces cerevisiae YGR207C   Cir1p
Schmidtea mediterranea mk4.000427.04   Electron transfer flavoprotein subunit beta
Trypanosoma brucei gambiense Tbg972.11.50   electron transfer flavoprotein, putative
Trypanosoma brucei Tb927.11.180   electron transfer flavoprotein, putative
Trypanosoma congolense TcIL3000.11.60   electron transfer flavoprotein, putative
Trypanosoma cruzi TcCLB.504109.160   electron transfer flavoprotein, putative
Toxoplasma gondii TGME49_267820   electron transfer flavoprotein subunit beta, putative

Essentiality

LmjF.25.0120 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu3082 Mycobacterium tuberculosis essential nmpdr
b0041 Escherichia coli non-essential goodall
b1697 Escherichia coli non-essential goodall
CELE_F23C8.5 Caenorhabditis elegans embryonic lethal wormbase
TGME49_267820 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.25.0120 (Leishmania major), electron transfer flavoprotein, putative
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