Detailed view for LmjF.01.0810

Basic information

TDR Targets ID: 23072
Leishmania major, calcium/potassium channel (CAKC), putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.1965 | Length (AA): 1017 | MW (Da): 114241 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 7

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03493   Calcium-activated BK potassium channel alpha subunit
PF07885   Ion channel

Gene Ontology

Mouse over links to read term descriptions.
GO:0016020   membrane  
GO:0015269   calcium-activated potassium channel activity  
GO:0006813   potassium ion transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
66 273 1orq (C) 31 237 15.00 0 0.07 0.42 -0.87
184 272 2a9h (A) 25 115 19.00 0.0000017 0.04 0.5 -2.46
222 385 1lnq (A) 49 189 36.00 0.0000098 0.74 0.21 0.57
185 275 2qks (A) 42 135 18.00 0 0.02 0.221579 -0.06
189 268 3ldc (A) 21 95 31.00 0 0.15 0.328763 -0.15
222 385 1lnq (A) 49 189 36.00 0.000051 0.98 0.124259 1.66
283 1016 3naf (A) 333 1054 18.00 0 0.99 0.568831 1.09
488 551 4xtt (A) 151 214 16.00 0.07 0.33 0.20963 0.03
594 713 2mqa (A) 23 138 16.00 0 0.01 0.244094 -0.22
885 960 1exb (E) 49 121 30.00 0.35 0.18 0.15573 0.78

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_142669)

Species Accession Gene Product
Dictyostelium discoideum DDB_G0275169   hypothetical protein
Leishmania braziliensis LbrM.01.0770   calcium/potassium channel (CAKC), putative
Leishmania donovani LdBPK_010830.1   calcium/potassium channel (CAKC), putative
Leishmania infantum LinJ.01.0830   calcium/potassium channel (CAKC), putative
Leishmania major LmjF.01.0810   calcium/potassium channel (CAKC), putative
Leishmania mexicana LmxM.01.0810   calcium/potassium channel (CAKC), putative
Trypanosoma brucei gambiense Tbg972.9.2540   calcium/potassium channel (CAKC), putative
Trypanosoma brucei Tb927.9.4820   calcium/potassium channel (CAKC), putative
Trypanosoma congolense TcIL3000_0_21040   calcium/potassium channel (CAKC), putative
Trypanosoma cruzi TcCLB.511585.220   calcium/potassium channel (CAKC), putative
Trypanosoma cruzi TcCLB.510155.210   calcium/potassium channel (CAKC), putative

Essentiality

LmjF.01.0810 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.160.3380 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.160.3380 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.160.3380 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb09.160.3380 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.01.0810 (Leishmania major), calcium/potassium channel (CAKC), putative
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