Detailed view for Wbm0128

Basic information

TDR Targets ID: 232817
Wolbachia endosymbiont of Brugia malayi, membrane associated signal transduction histidine kinase
Source Database / ID:  GenBank

pI: 6.8261 | Length (AA): 475 | MW (Da): 54858 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 2

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00512   His Kinase A (phospho-acceptor) domain
PF02518   Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016772   transferase activity, transferring phosphorus-containing groups  
GO:0016020   membrane  
GO:0005524   ATP binding  
GO:0004871   signal transducer activity  
GO:0004673   protein histidine kinase activity  
GO:0000155   two-component sensor activity  
GO:0018106   peptidyl-histidine phosphorylation  
GO:0016310   phosphorylation  
GO:0007165   signal transduction  

Metabolic Pathways

Two-component system (KEGG)

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
222 467 2c2a (A) 233 481 30.00 0 1 0.79 -0.1
222 466 2c2a (A) 233 480 33.00 0 1 0.92 -0.2
314 462 1id0 (A) 335 476 19.00 0.0000000000024 1 0.64 -1.09

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127065)

Species Accession Gene Product
Dictyostelium discoideum DDB_G0279913   HisK family protein kinase
Dictyostelium discoideum DDB_G0277887   HisK family protein kinase
Dictyostelium discoideum DDB_G0277845   HisK family protein kinase
Dictyostelium discoideum DDB_G0274191   HisK family protein kinase
Dictyostelium discoideum DDB_G0269204   HisK family protein kinase
Dictyostelium discoideum DDB_G0276143   HisK family protein kinase
Escherichia coli b2370   hybrid sensory histidine kinase in two-component regulatory system with EvgA
Schmidtea mediterranea mk4.035443.02  
Schmidtea mediterranea mk4.054018.00  
Wolbachia endosymbiont of Brugia malayi Wbm0128   membrane associated signal transduction histidine kinase

Essentiality

Wbm0128 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b2370 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

No enough druggable targets predicted through repurposing network model to make a plot

Putative Drugs List

Compound Raw Global Species
0.0023 0.4737 0

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Wbm0128 (Wolbachia endosymbiont of Brugia malayi), membrane associated signal transduction histidine kinase
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