Detailed view for Wbm0299

Basic information

TDR Targets ID: 232975
Wolbachia endosymbiont of Brugia malayi, replicative DNA helicase

Source Database / ID: GenBank / Wbm0299

pI: 7.3837 | Length (AA): 483 | MW (Da): 54775 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Pfam domains

PF00772   DnaB-like helicase N terminal domain
PF03796   DnaB-like helicase C terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0003677   DNA binding  
GO:0005524   ATP binding  
GO:0003678   DNA helicase activity  
GO:0006260   DNA replication  

Metabolic Pathways

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
18 477 2r6a (A) 8 441 38.00 0 1 1.31 0.09
20 477 2q6t (A) 6 440 39.00 0 1 1.36 -0.34
21 479 2r6e (A) 11 437 42.00 0 1 1.24 0.16
21 121 1b79 (A) 27 127 31.00 0 1 0.72 -1.9

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_131890)

Species Accession Gene Product
Chlamydia trachomatis CT_497   replicative DNA helicase
Escherichia coli b4052   replicative DNA helicase
Mycobacterium leprae ML2680c   PROBABLE REPLICATIVE DNA HELICASE DNAB replicative DNA helicase
Mycobacterium tuberculosis Rv0058   Probable replicative DNA helicase DnaB
Mycobacterium ulcerans MUL_0075   replicative DNA helicase DnaB
Schistosoma mansoni Smp_110860   Replicative DNA helicase
Schmidtea mediterranea mk4.039420.00  
Treponema pallidum TP0058   replicative DNA helicase (dnaB)
Wolbachia endosymbiont of Brugia malayi Wbm0299 this record   replicative DNA helicase

Essentiality

Wbm0299 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu58 Mycobacterium tuberculosis essential nmpdr
b4052 Escherichia coli essential goodall
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?
In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
 
In any case, if you have information about papers containing relevant validation data for this target, please log in using your TDR Targets username and password and send them to us using the corresponding form in this page (only visible to registered users) or contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Bacillus anthracis Replicative DNA helicase Compounds References
Staphylococcus aureus DnaC helicase Compounds References
Mycobacterium tuberculosis Probable replicative DNA helicase DnaB Compounds References
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG12228) Replicative DNA helicase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model

Ranking Plot

No enough druggable targets predicted through repurposing network model to make a plot

Putative Drugs List


Compound Raw Global Species
0.2603 0.7676 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier Wbm0299 (Wolbachia endosymbiont of Brugia malayi), replicative DNA helicase
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