Detailed view for Wbm0605

Basic information

TDR Targets ID: 233056
Wolbachia endosymbiont of Brugia malayi, leucyl-tRNA synthetase
Source Database / ID:  GenBank

pI: 7.2653 | Length (AA): 865 | MW (Da): 98820 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00133   tRNA synthetases class I (I, L, M and V)
PF08264   Anticodon-binding domain of tRNA
PF09334   tRNA synthetases class I (M)
PF13603   Leucyl-tRNA synthetase, Domain 2

Gene Ontology

Mouse over links to read term descriptions.
GO:0002161   GO:aminoacyl-tRNA editing activity  

GO:0005737   cytoplasm  
GO:0005524   ATP binding  
GO:0004823   leucine-tRNA ligase activity  
GO:0004812   aminoacyl-tRNA ligase activity  
GO:0000166   nucleotide binding  
GO:0006429   leucyl-tRNA aminoacylation  
GO:0006418   tRNA aminoacylation for protein translation  
GO:0006412   translation  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 865 1ffy (A) 16 862 20.00 0 1 0.84 1.17
2 864 2bte (A) 3 875 42.00 0 1 1.42 -0.18
2 804 2v0c (A) 3 813 42.00 0 1 1.33 -0.58
2 803 2v0c (A) 3 812 43.00 0 1 1.38 -0.46

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127675)

Species Accession Gene Product
Arabidopsis thaliana AT4G04350   tRNA synthetase class I (I, L, M and V) family protein
Babesia bovis BBOV_IV002370   leucyl-tRNA synthetase, putative
Brugia malayi Bm1_41800   protein ZK524.3
Candida albicans CaO19.13128   potential tRNA synthetase similar to S. cerevisiae NAM2 (YLR382C) Leucyl-tRNA synthetase
Candida albicans CaO19.5705   potential tRNA synthetase similar to S. cerevisiae NAM2 (YLR382C) Leucyl-tRNA synthetase
Caenorhabditis elegans CELE_ZK524.3   Protein LARS-2, isoform C
Chlamydia trachomatis CT_209   leucine--tRNA ligase
Dictyostelium discoideum DDB_G0291346   leucine-tRNA ligase
Drosophila melanogaster Dmel_CG7479   Leucyl-tRNA synthetase
Escherichia coli b0642   leucyl-tRNA synthetase
Echinococcus granulosus EgrG_000861400   leucyl tRNA synthetase
Echinococcus multilocularis EmuJ_000861400   leucyl tRNA synthetase
Homo sapiens ENSG00000011376   leucyl-tRNA synthetase 2, mitochondrial
Loa Loa (eye worm) LOAG_06912   hypothetical protein
Mycobacterium leprae ML0032   Probable leucyl-tRNA synthetase LeuS (Leucine--tRNA ligase)
Mus musculus ENSMUSG00000035202   leucyl-tRNA synthetase, mitochondrial
Mycobacterium tuberculosis Rv0041   Probable leucyl-tRNA synthetase LeuS (leucine--tRNA ligase) (LEURS)
Mycobacterium ulcerans MUL_0056   leucyl-tRNA synthetase
Neospora caninum NCLIV_039310   leucyl-tRNA synthetase, putative
Oryza sativa 4326033   Os01g0120300
Plasmodium berghei PBANKA_0702500   leucine--tRNA ligase, putative
Plasmodium falciparum PF3D7_0828200   leucine--tRNA ligase, putative
Plasmodium knowlesi PKNH_1322300   leucine--tRNA ligase, putative
Plasmodium yoelii PY05778   Leucyl-tRNA synthetase-related
Saccharomyces cerevisiae YLR382C   leucine--tRNA ligase NAM2
Schistosoma japonicum Sjp_0056410   Probable leucyl-tRNA synthetase, mitochondrial precursor, putative
Schistosoma japonicum Sjp_0077090   ko:K01869 leucyl-tRNA synthetase [EC6.1.1.4], putative
Schistosoma mansoni Smp_122780   leucyl-tRNA synthetase
Schmidtea mediterranea mk4.018836.00   Probable leucineALQ-tRNA ligase, mitochondrial
Schmidtea mediterranea mk4.003119.00   Probable leucineALQ-tRNA ligase, mitochondrial
Schmidtea mediterranea mk4.003119.01  
Toxoplasma gondii TGME49_266730   leucyl-tRNA synthetase (LeuRS2)
Toxoplasma gondii TGME49_251470   hypothetical protein
Treponema pallidum TP0586   leucyl-tRNA synthetase
Theileria parva TP03_0416   leucyl-tRNA synthetase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0605   leucyl-tRNA synthetase

Essentiality

Wbm0605 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b0642 Escherichia coli essential goodall
CELE_ZK524.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_ZK524.3 Caenorhabditis elegans slow growth wormbase
PBANKA_0702500 Plasmodium berghei Essential plasmo
TGME49_251470 Toxoplasma gondii Probably essential sidik
TGME49_266730 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

No enough druggable targets predicted through repurposing network model to make a plot

Putative Drugs List

Compound Raw Global Species
0.0049 0.2985 0

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Wbm0605 (Wolbachia endosymbiont of Brugia malayi), leucyl-tRNA synthetase
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