Detailed view for Wbm0621

Basic information

TDR Targets ID: 233247
Wolbachia endosymbiont of Brugia malayi, NADH:ubiquinone oxidoreductase chain N
Source Database / ID:  GenBank

pI: 8.1083 | Length (AA): 465 | MW (Da): 51678 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 14

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00361   Proton-conducting membrane transporter

Gene Ontology

Mouse over links to read term descriptions.
GO:0008137   NADH dehydrogenase (ubiquinone) activity  
GO:0055114   oxidation reduction  
GO:0042773   ATP synthesis coupled electron transport  

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 418 1fft (A) 109 517 17.00 0.00000073 0.05 0.82 1.4
265 451 1ehk (A) 24 210 17.00 0.000029 0.02 0.34 0.99

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_129510)

Species Accession Gene Product
Arabidopsis thaliana ArthCp068   NADH dehydrogenase subunit 2
Arabidopsis thaliana AT2G07689   NADH-ubiquinone/plastoquinone (complex I) protein
Arabidopsis thaliana ArthCp086   NADH dehydrogenase subunit 2
Arabidopsis thaliana ArthMp026   NADH dehydrogenase subunit 2
Candida albicans CaalfMp09   NADH dehydrogenase subunit 2
Dictyostelium discoideum DidioMp03   NADH dehydrogenase subunit 2
Escherichia coli b2276   NADH:ubiquinone oxidoreductase, membrane subunit N
Homo sapiens 4536   MTND2
Mus musculus 17717   NADH dehydrogenase subunit 2
Mycobacterium tuberculosis Rv3158   Probable NADH dehydrogenase I (chain N) NuoN (NADH-ubiquinone oxidoreductase chain N)
Mycobacterium ulcerans MUL_2472   NADH dehydrogenase subunit N
Oryza sativa 6450140   NADH dehydrogenase subunit 2
Oryza sativa 9272191   Os07g0467900
Oryza sativa 3131397   NADH dehydrogenase subunit 2
Oryza sativa 3131396   NADH dehydrogenase subunit 2
Schmidtea mediterranea mk4.053440.03  
Wolbachia endosymbiont of Brugia malayi Wbm0621   NADH:ubiquinone oxidoreductase chain N

Essentiality

Wbm0621 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu3210 Mycobacterium tuberculosis non-essential nmpdr
b2276 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens MTND2 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Wbm0621 (Wolbachia endosymbiont of Brugia malayi), NADH:ubiquinone oxidoreductase chain N
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