Detailed view for Bm1_53220

Basic information

TDR Targets ID: 233890
Brugia malayi, Iron-sulfur cluster assembly accessory protein

Source Database / ID:  GenBank

pI: 9.159 | Length (AA): 712 | MW (Da): 81438 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01521   Iron-sulphur cluster biosynthesis
PF06003   Survival motor neuron protein (SMN)
PF08585   RecQ mediated genome instability protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0097428   GO:protein maturation by iron-sulfur cluster transfer  

GO:0005737   cytoplasm  
GO:0005634   nucleus  
GO:0005198   structural molecule activity  
GO:0003723   RNA binding  
GO:0003676   nucleic acid binding  
GO:0051536   iron-sulfur cluster binding  
GO:0006397   mRNA processing  
GO:0016226   iron-sulfur cluster assembly  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
9 165 3nbi (A) 20 204 36.00 0 1 0.516606 -0.5
455 518 4cpc (A) 139 204 20.00 0 0.02 0.358988 -1.52
471 508 2n72 (A) 31 69 55.00 0.013 0.22 0.744971 -1.48
527 571 4a4f (A) 73 119 40.00 0.012 0.95 0.580802 -0.71
599 712 2d2a (A) 9 122 34.00 0.000000000049 1 0.641212 -0.51
601 698 1nwb (A) 3 101 30.00 0 0.94 0.51174 -0.44
601 712 2apn (A) 4 114 36.00 0 0.75 0.495403 0.16
606 701 1r94 (A) 1 96 30.00 0.0000000036 0.99 0.595431 -0.7
610 712 1x0g (A) 4 109 33.00 0.00000086 0.99 0.550263 -0.55

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127222)

Species Accession Gene Product
Arabidopsis thaliana AT2G36260   Iron-sulfur assembly protein IscA-like 3
Arabidopsis thaliana AT1G10500   Iron-sulfur assembly protein IscA
Arabidopsis thaliana AT2G16710   Iron-sulphur cluster biosynthesis family protein
Babesia bovis BBOV_III007290   HesB-like domain containing protein
Brugia malayi Bm1_53220   Iron-sulfur cluster assembly accessory protein
Candida albicans CaO19.5521   similar to Iron Sulfur cluster Assembly protein
Candida albicans CaO19.12967   similar to Iron Sulfur cluster Assembly protein
Caenorhabditis elegans CELE_Y39B6A.3   Protein Y39B6A.3, isoform A
Dictyostelium discoideum DDB_G0280173   hypothetical protein
Drosophila melanogaster Dmel_CG8198   lethal (1) G0136
Escherichia coli b2528   FeS cluster assembly protein
Escherichia coli b1684   Fe-S cluster assembly protein
Echinococcus granulosus EgrG_000221700   FeS cluster biogenesis
Echinococcus multilocularis EmuJ_000221700   FeS cluster biogenesis
Homo sapiens ENSG00000135070   iron-sulfur cluster assembly 1
Leishmania braziliensis LbrM.16.0840   hypothetical protein, conserved
Leishmania donovani LdBPK_160830.1   Iron-sulfur assembly protein 1, putative
Leishmania infantum LinJ.16.0830   hypothetical protein, conserved
Leishmania major LmjF.16.0830   hypothetical protein, conserved
Leishmania mexicana LmxM.16.0830   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_06869   hypothetical protein
Loa Loa (eye worm) LOAG_06870   hypothetical protein
Mus musculus ENSMUSG00000044792   iron-sulfur cluster assembly 1 homolog (S. cerevisiae)
Neospora caninum NCLIV_013550   hypothetical protein
Neospora caninum NCLIV_006830   HesB-like domain containing protein, putative
Oryza sativa 9272545   Os01g0105800
Oryza sativa 4352230   Os12g0485500
Onchocerca volvulus OVOC6059  
Plasmodium berghei PBANKA_1216000   iron-sulfur assembly protein, putative
Plasmodium falciparum PF3D7_0322500   iron-sulfur assembly protein, putative
Plasmodium knowlesi PKNH_1010200   iron-sulfur assembly protein, putative
Plasmodium knowlesi PKNH_0818300   iron-sulfur assembly protein, putative
Plasmodium vivax PVX_095105   iron-sulfur assembly protein, putative
Plasmodium vivax PVX_080115   iron-sulfur cluster assembly accessory protein, putative
Plasmodium yoelii PY01258   HesB-like domain protein-related
Saccharomyces cerevisiae YLL027W   Isa1p
Schistosoma japonicum Sjp_0081100   Iron-sulfur cluster assembly 1 homolog, mitochondrial precursor, putative
Schistosoma mansoni Smp_045150   hypothetical protein
Schmidtea mediterranea mk4.003587.00   Iron-sulfur cluster assembly 1 homolog, mitochondrial
Trypanosoma brucei gambiense Tbg972.8.5510   hypothetical protein, conserved
Trypanosoma brucei Tb927.8.5540   Iron-sulfur assembly protein 1
Trypanosoma congolense TcIL3000_8_5350   Iron-sulfur assembly protein 1, putative
Trypanosoma cruzi TcCLB.507083.70   Iron-sulfur assembly protein 1, putative
Trypanosoma cruzi TcCLB.509901.130   Iron-sulfur assembly protein 1, putative
Toxoplasma gondii TGME49_297925   HesB-like domain-containing protein
Toxoplasma gondii TGME49_213810   iron-sulfur cluster protein ISCA
Theileria parva TP04_0636   hypothetical protein
Wolbachia endosymbiont of Brugia malayi Wbm0757   HesB/YadR/YfhF family protein

Essentiality

Bm1_53220 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.5540 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.5540 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.5540 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.8.5540 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b1684 Escherichia coli non-essential goodall
b2528 Escherichia coli non-essential goodall
CELE_Y39B6A.3 Caenorhabditis elegans embryonic arrest wormbase
CELE_Y39B6A.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y39B6A.3 Caenorhabditis elegans larval arrest wormbase
CELE_Y39B6A.3 Caenorhabditis elegans slow growth wormbase
TGME49_213810 Toxoplasma gondii Probably essential sidik
TGME49_297925 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier Bm1_53220 (Brugia malayi), Iron-sulfur cluster assembly accessory protein
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