Detailed view for LmjF.27.1310

Basic information

TDR Targets ID: 23814
Leishmania major, arginyl-tRNA synthetase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.5701 | Length (AA): 692 | MW (Da): 78250 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00750   tRNA synthetases class I (R)
PF03485   Arginyl tRNA synthetase N terminal domain
PF05746   DALR anticodon binding domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005737   cytoplasm  
GO:0005524   ATP binding  
GO:0004814   arginine-tRNA ligase activity  
GO:0004812   aminoacyl-tRNA ligase activity  
GO:0000166   nucleotide binding  
GO:0006420   arginyl-tRNA aminoacylation  
GO:0006418   tRNA aminoacylation for protein translation  
GO:0006412   translation  

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
8 692 1f7u (A) 29 607 23.00 0 1 1.06 -0.33
10 692 1iq0 (A) 2 592 22.00 0 1 1.09 0.1
3 692 4r3z (B) 69 660 40.00 0 1 1.16671 0.81
5 692 4q2y (A) 0 588 44.00 0 1 1.17382 0.81
7 114 3gdz (A) 0 102 26.00 0 0.98 0.485269 -1.03
8 691 4oby (A) 1 576 40.00 0 1 1.18704 0.32

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_126752)

Species Accession Gene Product
Arabidopsis thaliana AT1G66530   arginyl-tRNA synthetase, class Ic
Arabidopsis thaliana AT4G26300   Arginyl-tRNA synthetase, class Ic
Babesia bovis BBOV_I004390   arginyl-tRNA synthetase, putative
Babesia bovis BBOV_II002780   arginyl-tRNA synthetase, putative
Brugia malayi Bm1_42070   arginyl-tRNA synthetase family protein
Brugia malayi Bm1_27000   arginyl aa-tRNA synthetase protein 1
Candida albicans CaO19.10849   likely Arginyl tRNA synthetase similar to S. cerevisiae YDR341C
Candida albicans CaO19.3341   likely Arginyl tRNA synthetase similar to S. cerevisiae YDR341C
Caenorhabditis elegans CELE_C29H12.1   Protein RARS-2
Caenorhabditis elegans CELE_F26F4.10   Protein RARS-1, isoform C
Cryptosporidium hominis Chro.50402   RIKEN cDNA 2610011N19 gene
Cryptosporidium parvum cgd5_4400   RIKEN cDNA 2610011N19 gen
Chlamydia trachomatis CT_454   arginine--tRNA ligase
Dictyostelium discoideum DDB_G0287803   arginine-tRNA ligase
Dictyostelium discoideum DDB_G0272867   arginine-tRNA ligase
Drosophila melanogaster Dmel_CG9020   Arginyl-tRNA synthetase
Drosophila melanogaster Dmel_CG10092   CG10092 gene product from transcript CG10092-RB
Escherichia coli b1876   arginyl-tRNA synthetase
Echinococcus granulosus EgrG_000348100   arginyl tRNA synthetase cytoplasmic
Echinococcus granulosus EgrG_000095100   arginyl tRNA synthetase
Entamoeba histolytica EHI_127020   arginyl-tRNA synthetase
Echinococcus multilocularis EmuJ_000095100   arginyl tRNA synthetase
Echinococcus multilocularis EmuJ_000348100   arginyl tRNA synthetase, cytoplasmic
Giardia lamblia GL50803_10521   Arginyl-tRNA synthetase
Homo sapiens ENSG00000113643   arginyl-tRNA synthetase
Homo sapiens ENSG00000146282   arginyl-tRNA synthetase 2, mitochondrial
Leishmania braziliensis LbrM.27.1450   arginyl-tRNA synthetase, putative
Leishmania donovani LdBPK_271230.1   arginyl-tRNA synthetase, putative
Leishmania infantum LinJ.27.1230   arginyl-tRNA synthetase, putative
Leishmania major LmjF.27.1310   arginyl-tRNA synthetase, putative
Leishmania mexicana LmxM.27.1310   arginyl-tRNA synthetase, putative
Loa Loa (eye worm) LOAG_02298   hypothetical protein
Loa Loa (eye worm) LOAG_00798   arginyl aa-tRNA synthetase 1
Mycobacterium leprae ML1127   PROBABLE ARGINYL-TRNA SYNTHETASE ARGS (ARGRS) (Arginine--tRNA ligase)
Mus musculus ENSMUSG00000018848   arginyl-tRNA synthetase
Mus musculus ENSMUSG00000028292   arginyl-tRNA synthetase 2, mitochondrial
Mycobacterium tuberculosis Rv1292   Probable arginyl-tRNA synthetase ArgS (ARGRS) (arginine--tRNA ligase)
Mycobacterium ulcerans MUL_3972   arginyl-tRNA synthetase
Neospora caninum NCLIV_011900   Arginyl-tRNA synthetase (EC 6.1.1.19), related
Oryza sativa 4324056   Os01g0158500
Oryza sativa 4337898   Os05g0163000
Plasmodium berghei PBANKA_1434200   arginine--tRNA ligase, putative
Plasmodium falciparum PF3D7_1218600   arginine--tRNA ligase
Plasmodium knowlesi PKNH_1438200   arginine--tRNA ligase, putative
Plasmodium vivax PVX_123597   arginyl-tRNA synthetase, putative
Plasmodium yoelii PY01800   arginyl-tRNA synthetase, putative
Saccharomyces cerevisiae YHR091C   arginine--tRNA ligase MSR1
Saccharomyces cerevisiae YDR341C   arginine--tRNA ligase
Schistosoma japonicum Sjp_0038640   ko:K01887 arginyl-tRNA synthetase [EC6.1.1.19], putative
Schistosoma mansoni Smp_133280   arginyl-tRNA synthetase
Schmidtea mediterranea mk4.009862.02   Probable arginineALQ-tRNA ligase, mitochondrial
Schmidtea mediterranea mk4.011011.00   Probable arginineALQ-tRNA ligase, mitochondrial
Schmidtea mediterranea mk4.001807.06   Probable arginineALQ-tRNA ligase, cytoplasmic
Trypanosoma brucei gambiense Tbg972.11.2170   arginyl-tRNA synthetase, putative
Trypanosoma brucei Tb927.11.1990   arginyl-tRNA synthetase, putative
Trypanosoma cruzi TcCLB.508355.320   arginyl-tRNA synthetase, putative
Treponema pallidum TP0831   arginyl-tRNA synthetase
Theileria parva TP01_1024   arginyl-tRNA synthetase, putative
Theileria parva TP04_0389   arginyl-tRNA synthetase, putative
Trichomonas vaginalis TVAG_324610   arginyl-tRNA synthetase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0205   arginyl-tRNA synthetase

Essentiality

LmjF.27.1310 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb11.46.0008 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.46.0008 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.46.0008 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.46.0008 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b1876 Escherichia coli essential goodall
CELE_F26F4.10 Caenorhabditis elegans embryonic lethal wormbase
CELE_F26F4.10 Caenorhabditis elegans larval arrest wormbase
CELE_F26F4.10 Caenorhabditis elegans slow growth wormbase
CELE_F26F4.10 Caenorhabditis elegans sterile wormbase
CELE_C29H12.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_C29H12.1 Caenorhabditis elegans slow growth wormbase
CELE_C29H12.1 Caenorhabditis elegans sterile wormbase
YDR341C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1434200 Plasmodium berghei Essential plasmo
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens arginyl-tRNA synthetase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier LmjF.27.1310 (Leishmania major), arginyl-tRNA synthetase, putative
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