Detailed view for LmjF.36.5180

Basic information

TDR Targets ID: 23861
Leishmania major, aldehyde dehydrogenase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.8939 | Length (AA): 532 | MW (Da): 57735 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00171   Aldehyde dehydrogenase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016620   oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor  
GO:0016491   oxidoreductase activity  
GO:0055114   oxidation reduction  
GO:0008152   metabolic process  
GO:0006081   aldehyde metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 483 1uxt (A) 31 501 23.00 0 1 1.19 -0.78
3 469 2bhq (A) 45 516 25.00 0 1 1.28 -1.07
6 469 1a4s (A) 39 496 31.00 0 1 1.36 -1.05
1 486 3iwk (A) 21 502 29.00 0.00000000013 1 1.23243 0.06
4 465 2imp (A) 23 475 34.00 0 1 1.25912 -0.17
4 469 4cbb (A) 23 483 26.00 0.000000000006 1 1.24484 -0.64
9 463 4i9b (A) 32 484 34.00 0 1 1.32196 -0.53
9 452 5klo (A) 39 483 30.00 0 1 1.24229 -0.44

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_164857)

Species Accession Gene Product
Leishmania braziliensis LbrM.35.5430   aldehyde dehydrogenase, putative
Leishmania donovani LdBPK_365410.1   aldehyde dehydrogenase, putative
Leishmania infantum LinJ.36.5410   aldehyde dehydrogenase, putative
Leishmania major LmjF.36.5180   aldehyde dehydrogenase, putative
Leishmania mexicana LmxM.36.5180   aldehyde dehydrogenase, putative
Mycobacterium tuberculosis Rv1731   Possible succinate-semialdehyde dehydrogenase [NADP+] dependent (SSDH) GabD2
Mycobacterium ulcerans MUL_3430   succinate-semialdehyde dehydrogenase [NADP+] dependent, PutA_2
Mycobacterium ulcerans MUL_3190   succinate-semialdehyde dehydrogenase
Mycobacterium ulcerans MUL_2361   succinate-semialdehyde dehydrogenase [NADP+] dependent, GabD1
Trypanosoma cruzi TcCLB.506155.60   aldehyde dehydrogenase, putative

Essentiality

LmjF.36.5180 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu1761 Mycobacterium tuberculosis non-essential nmpdr
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Aldehyde dehydrogenase 519 aa 27.8% 468 aa Compounds References
Rattus norvegicus Aldehyde dehydrogenase 1A1 501 aa 29.6% 466 aa Compounds References
Rattus norvegicus Aldehyde dehydrogenase, cytosolic 1 501 aa 30.1% 468 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.36.5180 (Leishmania major), aldehyde dehydrogenase, putative
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