TDR Targets

Detailed view for LmjF.33.0802

Basic information

TDR Targets ID: 23905
Leishmania major, beta tubulin
Source Database / ID: TriTrypDB GeneDB

pI: 4.4459 | Length (AA): 443 | MW (Da): 49740 | Paralog Number: 17

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Network

Pfam domains

PF00091 Tubulin/FtsZ family, GTPase domain
PF03953 Tubulin C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.

GO:0043234 protein complex
GO:0005874 microtubule
GO:0005525 GTP binding
GO:0005200 structural constituent of cytoskeleton
GO:0005198 structural molecule activity
GO:0003924 GTPase activity
GO:0051258 protein polymerization
GO:0007018 microtubule-based movement
GO:0007017 microtubule-based process

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent	Ranking	Stage	Dataset
NA% percentile		amastigotes, metacyclic.	Fernandes MC

Show/Hide expression data references

Fernandes MC

Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_126611)

Species	Accession	Gene Product
Arabidopsis thaliana	AT5G44340	tubulin beta-4 chain
Arabidopsis thaliana	AT1G75780	tubulin beta-1 chain
Arabidopsis thaliana	AT2G29550	tubulin beta-7 chain
Arabidopsis thaliana	AT5G12250	beta-6 tubulin
Arabidopsis thaliana	AT4G20890	tubulin beta-9 chain
Arabidopsis thaliana	AT5G23860	tubulin beta-8 chain
Arabidopsis thaliana	AT5G62700	tubulin beta-2/beta-3 chain
Arabidopsis thaliana	AT5G62690	tubulin beta-2/beta-3 chain
Arabidopsis thaliana	AT1G20010	tubulin beta-5 chain
Babesia bovis	BBOV_III004850	tubulin beta chain
Brugia malayi	Bm1_07675	Tubulin beta-1 chain
Brugia malayi	Bm1_25780	beta-tubulin, identical
Caenorhabditis elegans	CELE_C54C6.2	Protein BEN-1
Caenorhabditis elegans	CELE_C36E8.5	Protein TBB-2
Caenorhabditis elegans	CELE_B0272.1	Protein TBB-4
Caenorhabditis elegans	CELE_K01G5.7	Protein TBB-1
Caenorhabditis elegans	CELE_ZK154.3	Protein MEC-7
Cryptosporidium hominis	Chro.60548	beta-catenin-like repeat protein
Cryptosporidium parvum	cgd6_4760	tubulin beta chain
Dictyostelium discoideum	DDB_G0269196	beta tubulin
Drosophila melanogaster	Dmel_CG9277	beta-Tubulin at 56D
Drosophila melanogaster	Dmel_CG3401	beta-Tubulin at 60D
Drosophila melanogaster	Dmel_CG4869	beta-Tubulin at 97EF
Drosophila melanogaster	Dmel_CG9359	beta-Tubulin at 85D
Echinococcus granulosus	EgrG_000955100	Tubulin beta 2C chain
Echinococcus granulosus	EgrG_000069900	beta tubulin
Echinococcus granulosus	EgrG_000617000	Tubulin beta 2C chain
Echinococcus granulosus	EgrG_001126150	tubulin beta 4A class IVa
Echinococcus granulosus	EgrG_000569000	tubulin subunit beta
Echinococcus granulosus	EgrG_000041100	Tubulin beta 2C chain
Echinococcus granulosus	EgrG_000202500	beta tubulin
Echinococcus granulosus	EgrG_000202600	Tubulin beta 2C chain
Echinococcus granulosus	EgrG_001081200	tubulin beta 1 chain
Echinococcus granulosus	EgrG_000672200	Tubulin beta 2C chain
Entamoeba histolytica	EHI_167010	tubulin family protein
Entamoeba histolytica	EHI_049920	tubulin family protein
Echinococcus multilocularis	EmuJ_001126150	tubulin, beta 4A class IVa
Echinococcus multilocularis	EmuJ_000672200	Tubulin beta 2C chain
Echinococcus multilocularis	EmuJ_000955100	Tubulin beta 2C chain
Echinococcus multilocularis	EmuJ_000202500	beta tubulin
Echinococcus multilocularis	EmuJ_000569000	tubulin subunit beta
Echinococcus multilocularis	EmuJ_000069900	beta tubulin
Echinococcus multilocularis	EmuJ_000617000	Tubulin beta 2C chain
Echinococcus multilocularis	EmuJ_000202600	Tubulin beta 2C chain
Echinococcus multilocularis	EmuJ_001081200	tubulin beta 1 chain
Echinococcus multilocularis	EmuJ_000041100	Tubulin beta 2C chain
Giardia lamblia	GL50803_101291	Beta tubulin
Giardia lamblia	GL50803_136020	Beta tubulin
Giardia lamblia	GL50803_136021	Beta tubulin
Homo sapiens	ENSG00000137267	tubulin, beta 2A class IIa
Homo sapiens	ENSG00000261456	tubulin, beta 8 class VIII
Homo sapiens	ENSG00000196230	tubulin, beta class I
Homo sapiens	ENSG00000104833	tubulin, beta 4A class IVa
Homo sapiens	ENSG00000258947	tubulin, beta 3 class III
Homo sapiens	ENSG00000258839	melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor)
Homo sapiens	ENSG00000101162	tubulin, beta 1 class VI
Homo sapiens	ENSG00000188229	tubulin, beta 4B class IVb
Homo sapiens	ENSG00000137285	tubulin, beta 2B class IIb
Homo sapiens	ENSG00000176014	tubulin, beta 6 class V
Leishmania braziliensis	LbrM.21.2150	beta tubulin
Leishmania braziliensis	LbrM.33.0960	beta-tubulin
Leishmania braziliensis	LbrM.33.0930	beta-tubulin
Leishmania braziliensis	LbrM.33.0920	beta-tubulin
Leishmania infantum	LinJ.08.1280	beta tubulin
Leishmania infantum	LinJ.08.1290	beta tubulin
Leishmania infantum	LinJ.21.2240	beta tubulin
Leishmania infantum	LinJ.33.0860	beta-tubulin
Leishmania major	LmjF.33.0816	beta tubulin
Leishmania major	LmjF.33.0812	beta tubulin
Leishmania major	LmjF.33.0806	beta tubulin
Leishmania major	LmjF.08.1230	beta tubulin
Leishmania major	LmjF.33.0804	beta tubulin
Leishmania major	LmjF.33.0802	beta tubulin
Leishmania major	LmjF.33.0808	beta tubulin
Leishmania major	LmjF.33.0792	beta tubulin
Leishmania major	LmjF.33.0819	beta tubulin
Leishmania major	LmjF.33.0818	beta tubulin
Leishmania major	LmjF.21.1860	beta tubulin
Leishmania major	LmjF.33.0800	beta tubulin
Leishmania major	LmjF.33.0814	beta tubulin
Leishmania major	LmjF.33.0798	beta tubulin
Leishmania major	LmjF.33.0796	beta tubulin
Leishmania major	LmjF.33.0810	beta tubulin
Leishmania major	LmjF.33.0794	beta tubulin
Leishmania major	LmjF.33.0820	beta tubulin
Leishmania mexicana	LmxM.32.0792	beta tubulin
Leishmania mexicana	LmxM.21.1860	beta tubulin
Leishmania mexicana	LmxM.32.0794	beta tubulin
Leishmania mexicana	LmxM.08.1171
Leishmania mexicana	LmxM.08.1230	beta tubulin
Loa Loa (eye worm)	LOAG_04418	tubulin beta chain
Loa Loa (eye worm)	LOAG_12815	BEN-1 protein
Loa Loa (eye worm)	LOAG_08711	tubulin beta-1 chain
Loa Loa (eye worm)	LOAG_11824	beta-tubulin
Loa Loa (eye worm)	LOAG_12555	BEN-1 protein
Loa Loa (eye worm)	LOAG_00602	tubulin beta-2A chain
Loa Loa (eye worm)	LOAG_10501	tubulin beta-4 chain
Mus musculus	ENSMUSG00000036752	tubulin, beta 4B class IVB
Mus musculus	ENSMUSG00000001525	tubulin, beta 5 class I
Mus musculus	ENSMUSG00000058672	tubulin, beta 2A class IIA
Mus musculus	ENSMUSG00000062591	tubulin, beta 4A class IVA
Mus musculus	ensembl-mmu:ENSMUSG00000001473	tubulin, beta 6 class V
Mus musculus	ENSMUSG00000045136	tubulin, beta 2B class IIB
Mus musculus	545486	tubulin, beta 1 class VI
Mus musculus	ENSMUSG00000062380	tubulin, beta 3 class III
Neospora caninum	NCLIV_005150	hypothetical protein
Neospora caninum	NCLIV_039100	hypothetical protein
Neospora caninum	NCLIV_049140	hypothetical protein
Oryza sativa	4333632	Os03g0661300
Oryza sativa	4341810	Os06g0671900
Oryza sativa	4328420	Os02g0167300
Oryza sativa	4331315	Os03g0105600
Oryza sativa	4327550	Os01g0805900
Oryza sativa	4338790	Os05g0413200
Oryza sativa	4326917	Os01g0282800
Oryza sativa	4334309	Os03g0780600
Plasmodium berghei	PBANKA_1206900	tubulin beta chain, putative
Plasmodium falciparum	PF3D7_1008700	tubulin beta chain
Plasmodium vivax	PVX_094635	tubulin beta chain, putative
Plasmodium yoelii	PY05711	tubulin beta chain
Saccharomyces cerevisiae	YFL037W	beta-tubulin
Schistosoma japonicum	Sjp_0027870	Tubulin beta-4 chain, putative
Schistosoma japonicum	Sjp_0060500	Tubulin beta chain, putative
Schistosoma japonicum	Sjp_0095680	Tubulin beta-7 chain, putative
Schistosoma japonicum	Sjp_0090690	ko:K07375 tubulin beta, putative
Schistosoma japonicum	Sjp_0068060	Tubulin beta chain, putative
Schistosoma japonicum	Sjp_0080560	Tubulin beta-1 chain, putative
Schistosoma japonicum	Sjp_0209650	Tubulin beta chain, putative
Schistosoma japonicum	Sjp_0080550	Tubulin beta chain, putative
Schistosoma japonicum	Sjp_0067680	Tubulin beta-1 chain, putative
Schistosoma japonicum	Sjp_0090950	ko:K07375 tubulin beta, putative
Schistosoma mansoni	Smp_078040	tubulin subunit beta
Schistosoma mansoni	Smp_079970	tubulin subunit beta
Schistosoma mansoni	Smp_192110	tubulin subunit beta
Schistosoma mansoni	Smp_079960	tubulin subunit beta
Schistosoma mansoni	Smp_035760	tubulin subunit beta
Schistosoma mansoni	Smp_030730	tubulin subunit beta
Schmidtea mediterranea	mk4.005007.03
Schmidtea mediterranea	mk4.002479.04
Schmidtea mediterranea	mk4.009333.02
Schmidtea mediterranea	mk4.004337.00
Schmidtea mediterranea	mk4.035087.00
Schmidtea mediterranea	mk4.000452.01	Tubulin beta-1 chain
Schmidtea mediterranea	mk4.000225.08
Schmidtea mediterranea	mk4.005538.05	Tubulin beta-2 chain
Schmidtea mediterranea	mk4.000157.13	Tubulin beta-1 chain
Schmidtea mediterranea	mk4.006183.00	Tubulin beta-2 chain
Schmidtea mediterranea	mk4.038724.00	Tubulin beta chain, putative
Schmidtea mediterranea	mk4.000299.16	Tubulin beta-2 chain
Schmidtea mediterranea	mk4.002542.01	Tubulin beta-2 chain
Schmidtea mediterranea	mk4.000536.06	Tubulin beta-2 chain
Schmidtea mediterranea	mk4.001487.01
Schmidtea mediterranea	mk4.055780.00	Tubulin beta chain, putative
Schmidtea mediterranea	mk4.000062.06	Tubulin beta-1 chain
Schmidtea mediterranea	mk4.002158.04	Tubulin beta-1 chain
Schmidtea mediterranea	mk4.009683.01
Schmidtea mediterranea	mk4.001602.00
Schmidtea mediterranea	mk4.004971.01	Tubulin beta-1 chain
Schmidtea mediterranea	mk4.007635.00
Schmidtea mediterranea	mk4.002409.02
Schmidtea mediterranea	mk4.000062.07	Tubulin beta-1 chain
Schmidtea mediterranea	mk4.000397.07
Trypanosoma brucei gambiense	Tbg972.1.1350	beta tubulin
Trypanosoma brucei gambiense	Tbg972.1.1370	beta tubulin
Trypanosoma brucei	Tb927.1.2350	beta tubulin
Trypanosoma brucei	Tb927.1.2330	beta tubulin
Trypanosoma brucei	Tb927.1.2390	beta tubulin
Trypanosoma brucei	Tb927.1.2370	beta tubulin
Trypanosoma congolense	TcIL3000_0_31240	beta tubulin
Trypanosoma cruzi	TcCLB.506563.40	beta tubulin, putative
Toxoplasma gondii	TGME49_221620	beta-tubulin, putative
Toxoplasma gondii	TGME49_266960	beta tubulin
Toxoplasma gondii	TGME49_212240	beta-1 tubulin, putative
Theileria parva	TP02_0464	tubulin beta chain, putative
Trichomonas vaginalis	TVAG_008680	tubulin epsilon chain, putative
Trichomonas vaginalis	TVAG_338530	tubulin epsilon chain, putative
Trichomonas vaginalis	TVAG_525430	tubulin gamma chain, putative
Trichomonas vaginalis	TVAG_456920	tubulin alpha chain, putative
Trichomonas vaginalis	TVAG_062880	tubulin, putative
Trichomonas vaginalis	TVAG_034440	tubulin beta chain, putative

Essentiality

LmjF.33.0802 has one or more orthologs with essentiality data

Gene/Ortholog	Organism	Phenotype	Source Study
Tb927.1.2330	Trypanosoma brucei	significant loss of fitness in bloodstream forms (3 days)	alsford
Tb927.1.2330	Trypanosoma brucei	significant loss of fitness in bloodstream forms (6 days)	alsford
Tb927.1.2330	Trypanosoma brucei	significant loss of fitness in procyclic forms	alsford
Tb927.1.2330	Trypanosoma brucei	significant loss of fitness in differentiation of procyclic to bloodstream forms	alsford
CELE_C54C6.2	Caenorhabditis elegans	embryonic lethal	wormbase
CELE_C54C6.2	Caenorhabditis elegans	larval arrest	wormbase
CELE_C54C6.2	Caenorhabditis elegans	slow growth	wormbase
CELE_C54C6.2	Caenorhabditis elegans	sterile	wormbase
CELE_ZK154.3	Caenorhabditis elegans	embryonic lethal	wormbase
CELE_ZK154.3	Caenorhabditis elegans	larval arrest	wormbase
CELE_ZK154.3	Caenorhabditis elegans	larval lethal	wormbase
CELE_ZK154.3	Caenorhabditis elegans	slow growth	wormbase
CELE_ZK154.3	Caenorhabditis elegans	sterile	wormbase
CELE_K01G5.7	Caenorhabditis elegans	embryonic lethal	wormbase
CELE_K01G5.7	Caenorhabditis elegans	larval arrest	wormbase
CELE_K01G5.7	Caenorhabditis elegans	slow growth	wormbase
CELE_K01G5.7	Caenorhabditis elegans	sterile	wormbase
CELE_C36E8.5	Caenorhabditis elegans	embryonic lethal	wormbase
CELE_C36E8.5	Caenorhabditis elegans	larval lethal	wormbase
CELE_C36E8.5	Caenorhabditis elegans	slow growth	wormbase
CELE_C36E8.5	Caenorhabditis elegans	sterile	wormbase
CELE_B0272.1	Caenorhabditis elegans	embryonic lethal	wormbase
CELE_B0272.1	Caenorhabditis elegans	slow growth	wormbase
YFL037W	Saccharomyces cerevisiae	inviable	yeastgenome
PBANKA_1206900	Plasmodium berghei	Essential	plasmo
TGME49_212240	Toxoplasma gondii	Probably non-essential	sidik
TGME49_266960	Toxoplasma gondii	Probably non-essential	sidik
TGME49_221620	Toxoplasma gondii	Probably non-essential	sidik
TGME49_212240	Toxoplasma gondii	Probably essential	sidik
TGME49_266960	Toxoplasma gondii	Probably essential	sidik
TGME49_221620	Toxoplasma gondii	Probably essential	sidik

Show/Hide essentiality data references

keio

Systematic single-gene knock-out mutants of E. coli K12

The Keio Collection

blattner

Systematic mutagenesis of the E. coli (MG1655) genome

J Bacteriol 2004, 186:4921-4930

nmpdr

Genome-scale essentiality datasets from published studies (M. tuberculosis)

National Microbial Pathogen Data Resource

shigen

Profiling of E. coli Chromosome (PEC)

National Institute of Genetics, Japan

goodall

The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis)

Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

yeastgenome

Systematic deletion of yeast genes

Saccharomyces Genome Database

plasmo

Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes.

Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

gerdes

Experimental determination and system-level analysis of essential genes in E. coli MG1655

Gerdes et al., J Bacteriol. 2003 185:5673-84

alsford

High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome

Genome Res 2011, 21:915-924

neb

C. elegans RNAi phenotypes

Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

sidik

A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes.

Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

wormbase

C. elegans RNAi experiments

WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity	Phenotypic quality	Occurs in	Occurs at	Evidence	Observed in	Drugs/Inhibitors
growth (GO:0040007)	decreased time (PATO:0000716)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from bioassay (ECO:0000094)	Leishmania amazonensis	No drug identifiers listed for this gene.
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 09/08/82. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; .		References:	2392684
growth (GO:0040007)	decreased time (PATO:0000716)	single cell organism (CARO:0000064)	amastigote (BTO:0000062)	inferred from bioassay (ECO:0000094)	Leishmania amazonensis	No drug identifiers listed for this gene.
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 09/08/82. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; .		References:	2392684
growth (GO:0040007)	decreased time (PATO:0000716)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from bioassay (ECO:0000094)	Leishmania mexicana	79195
Annotator:	aaronjr@u.washington.edu.	Comment:	chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied.		References:	2044859
morphology (PATO:0000051)	abnormal (PATO:0000460)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from visible phenotype (ECO:0000176)	Leishmania mexicana	79195
Annotator:	aaronjr@u.washington.edu.	Comment:	chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase II gene/enzyme was studied.		References:	2044859
growth (GO:0040007)	decreased time (PATO:0000716)	single cell organism (CARO:0000064)	amastigote (BTO:0000062)	inferred from bioassay (ECO:0000094)	Leishmania mexicana	79195
Annotator:	aaronjr@u.washington.edu.	Comment:	chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase II gene/enzyme was studied.		References:	2044859
cell differentiation (GO:0030154)	disrupted (PATO:0001507)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from bioassay (ECO:0000094)	Leishmania mexicana	79195
Annotator:	aaronjr@u.washington.edu.	Comment:	chemical inhibition with antimicrotubule agents leads to disrupted differentiation from promastigote to amastigote of Leishmania sp. in cell assay; unclear as to which topoisomerase II gene/enzyme was studied.		References:	2044859
growth (GO:0040007)	decreased time (PATO:0000716)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from bioassay (ECO:0000094)	Leishmania amazonensis	586034
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 95480-32-3. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase II gene/enzyme was studied.		References:	8468004
growth (GO:0040007)	decreased time (PATO:0000716)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from bioassay (ECO:0000094)	Leishmania amazonensis	13316 39854 79195 544264 551075 552236 554547 555991 557331 559236 559237 559604 561797 562909 564379 564831 568817 568820 568821 569525
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 29091-21-2. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase II gene/enzyme was studied.		References:	7818612
growth (GO:0040007)	decreased time (PATO:0000716)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from bioassay (ECO:0000094)	Leishmania major	39854 250338 536202
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 121-50-6; Drug: 394-25-2; Drug: 14371-82-5; Drug: 22227-59-4; Drug: 174824-16-9; Drug: 176177-41-6. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied.		References:	8849257
microtubule polymerization (GO:0046785)	decreased (PATO:0000468)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from specific protein inhibition (ECO:0000020)	Leishmania amazonensis	2976 8265 27599 67873 86876 250338 327961 371083 376038 543486 543487 559148 560712 572272 575226 588233
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 16037-91-5. chemical inhibition with antimicrotubule agents prevents mictotubule formation in vitro with purified tubulin and in live cells; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied.		References:	10029309
growth (GO:0040007)	decreased time (PATO:0000716)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from bioassay (ECO:0000094)	Leishmania amazonensis	2976 8265 27599 67873 86876 250338 327961 371083 376038 543486 543487 559148 560712 572272 575226 588233
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 16037-91-5. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied.		References:	10029309
microtubule polymerization (GO:0046785)	increased (PATO:0000470)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from bioassay (ECO:0000094)	Leishmania donovani	2976 8265 559148
Annotator:	aaronjr@u.washington.edu.	Comment:	chemical inhibition with microtubule stabilising agent forces mictotubule formation in live cells and interrupts cell cycle; unclear as to which topoisomerase II gene/enzyme was studied.		References:	10427726
growth (GO:0040007)	decreased time (PATO:0000716)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from bioassay (ECO:0000094)	Leishmania donovani	2976 8265 559148
Annotator:	aaronjr@u.washington.edu.	Comment:	chemical inhibition with microtubule stabilising agent forces mictotubule formation in live cells and interrupts cell cycle; unclear as to which topoisomerase II gene/enzyme was studied.		References:	10427726
growth (GO:0040007)	drug insensitive (PATO:0000528)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from bioassay (ECO:0000094)	Leishmania donovani	2976 8265 559148
Annotator:	aaronjr@u.washington.edu.	Comment:	chemical inhibition with microtubule stabilising agent generated cell line resistant to drug with a MDR phenotype; reversible with Ca2+-channel blocker verapamil; unclear as to which topoisomerase II gene/enzyme was studied.		References:	10427727
growth (GO:0040007)	drug insensitive (PATO:0000528)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from bioassay (ECO:0000094)	Leishmania infantum	37094 39854 79195 250338 551075 562909
Annotator:	aaronjr@u.washington.edu.	Comment:	chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied.		References:	10546848
microtubule polymerization (GO:0046785)	decreased (PATO:0000468)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from specific protein inhibition (ECO:0000020)	Leishmania donovani	2976 8265 80107 559148 576689 576690
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 313705-89-4; Drug: 157207-90-4. chemical inhibition with antimicrotubule agents leads to abnormal cell morphology, cell division, mitosis, organelle localization & organization, and slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase II gene/enzyme was studied.		References:	11071278
growth (GO:0040007)	decreased time (PATO:0000716)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from bioassay (ECO:0000094)	Leishmania donovani	2976 8265 80107 559148 576689 576690
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 313705-89-4; Drug: 157207-90-4. chemical inhibition with antimicrotubule agents leads to abnormal cell morphology, cell division, mitosis, organelle localization & organization, and slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase II gene/enzyme was studied.		References:	11071278
cytokinesis (GO:0000910)	discontinuous (PATO:0000690)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from visible phenotype (ECO:0000176)	Leishmania donovani	2976 8265 80107 559148 576689 576690
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 313705-89-4; Drug: 157207-90-4. chemical inhibition with antimicrotubule agents leads to abnormal cell morphology, cell division, mitosis, organelle localization & organization, and slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied.		References:	11071278
mitotic sister chromatid segregation (GO:0000070)	discontinuous (PATO:0000690)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from visible phenotype (ECO:0000176)	Leishmania donovani	2976 8265 80107 559148 576689 576690
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 313705-89-4; Drug: 157207-90-4. chemical inhibition with antimicrotubule agents leads to abnormal cell morphology, cell division, mitosis, organelle localization & organization, and slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied.		References:	11071278
organelle localization (GO:0051640)	discontinuous (PATO:0000690)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from visible phenotype (ECO:0000176)	Leishmania donovani	2976 8265 80107 559148 576689 576690
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 313705-89-4; Drug: 157207-90-4. chemical inhibition with antimicrotubule agents leads to abnormal cell morphology, cell division, mitosis, organelle localization & organization, and slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase II gene/enzyme was studied.		References:	11071278
morphology (PATO:0000051)	abnormal (PATO:0000460)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from visible phenotype (ECO:0000176)	Leishmania donovani	2976 8265 80107 559148 576689 576690
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 313705-89-4; Drug: 157207-90-4. chemical inhibition with antimicrotubule agents leads to abnormal cell morphology, cell division, mitosis, organelle localization & organization, and slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase II gene/enzyme was studied.		References:	11071278
growth (GO:0040007)	decreased time (PATO:0000716)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from anti-sense experiment (ECO:0000018)	Leishmania donovani	No drug identifiers listed for this gene.
Annotator:	aaronjr@u.washington.edu.	Comment:	Genetic disruption of the beta-tubulin gene product via anti-sense RNA was found to have antileishmanial activity; unclear as to which topoisomerase II gene/enzyme was studied.		References:	12097156
growth (GO:0040007)	decreased time (PATO:0000716)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from bioassay (ECO:0000094)	Leishmania donovani	12596 13316 15907 26907 78691 79195 527824 528258 531885 531886 532164 532165 532166 532195 532196 532197 532198 532199 532642 532643 532644 536969 536970 544264 552236 554547 559604 561797 564379 568817 568820 568821
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 10228-56-5; Drug: 36317-95-0; Drug: 62409-47-6; Drug: 62745-74-8; Drug: 204634-79-7; Drug: 483279-40-9; Drug: 483279-41-0; Drug: 483279-42-1; Drug: 483279-43-2; Drug: 483279-44-3; Drug: 483279-45-4; Drug: 483279-46-5; Drug: 483279-47-6; Drug: 88-91-5; Drug: 107-10-8; Drug: 1930-72-9; Drug: 97-08-5; Drug: 97-09-6; Drug: 62443-96-3; Drug: 483279-33-0; Drug: 483279-34-1; Drug: 483279-35-2; Drug: 483279-36-3; Drug: 483279-37-4; Drug: 483279-38-5; Drug: 483279-39-6. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase II gene/enzyme was studied.		References:	12161141
growth (GO:0040007)	decreased time (PATO:0000716)	single cell organism (CARO:0000064)	amastigote (BTO:0000062)	inferred from bioassay (ECO:0000094)	Leishmania donovani	12596 13316 15907 26907 78691 79195 527824 528258 531885 531886 532164 532165 532166 532195 532196 532197 532198 532199 532642 532643 532644 536969 536970 544264 552236 554547 559604 561797 564379 568817 568820 568821
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 10228-56-5; Drug: 36317-95-0; Drug: 62409-47-6; Drug: 62745-74-8; Drug: 204634-79-7; Drug: 483279-40-9; Drug: 483279-41-0; Drug: 483279-42-1; Drug: 483279-43-2; Drug: 483279-44-3; Drug: 483279-45-4; Drug: 483279-46-5; Drug: 483279-47-6; Drug: 88-91-5; Drug: 107-10-8; Drug: 1930-72-9; Drug: 97-08-5; Drug: 97-09-6; Drug: 62443-96-3; Drug: 483279-33-0; Drug: 483279-34-1; Drug: 483279-35-2; Drug: 483279-36-3; Drug: 483279-37-4; Drug: 483279-38-5; Drug: 483279-39-6. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied.		References:	12161141
microtubule polymerization (GO:0046785)	decreased (PATO:0000468)	in vitro (MI:0492)		inferred from specific protein inhibition (ECO:0000020)	Leishmania donovani	12596 13316 15907 26907 78691 79195 527824 528258 531885 531886 532164 532165 532166 532195 532196 532197 532198 532199 532642 532643 532644 536969 536970 544264 552236 554547 559604 561797 564379 568817 568820 568821
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 10228-56-5; Drug: 36317-95-0; Drug: 62409-47-6; Drug: 62745-74-8; Drug: 204634-79-7; Drug: 483279-40-9; Drug: 483279-41-0; Drug: 483279-42-1; Drug: 483279-43-2; Drug: 483279-44-3; Drug: 483279-45-4; Drug: 483279-46-5; Drug: 483279-47-6; Drug: 88-91-5; Drug: 107-10-8; Drug: 1930-72-9; Drug: 97-08-5; Drug: 97-09-6; Drug: 62443-96-3; Drug: 483279-33-0; Drug: 483279-34-1; Drug: 483279-35-2; Drug: 483279-36-3; Drug: 483279-37-4; Drug: 483279-38-5; Drug: 483279-39-6. chemical inhibition with antimicrotubule agents prevents mictotubule formation in vitro with purified tubulin; unclear as to which topoisomerase II gene/enzyme was studied.		References:	12161141
cell cycle arrest (GO:0007050)	abnormal (PATO:0000460)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from bioassay (ECO:0000094)	Leishmania donovani	79195
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 483279-40-9; Drug: 483279-44-3. chemical inhibition with antimicrotubule agents leads to cell cycle arrest in cell assay; unclear as to which topoisomerase II gene/enzyme was studied.		References:	14645662
growth (GO:0040007)	decreased time (PATO:0000716)	single cell organism (CARO:0000064)	amastigote (BTO:0000062)	inferred from bioassay (ECO:0000094)	Leishmania donovani	79195
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 19044-89-4; Drug: 19044-94-1; Drug: 483279-44-3; Drug: 483279-45-4; Drug: 619267-13-9; Drug: 619267-14-0; Drug: 619267-15-1; Drug: 619267-16-2; Drug: 619267-17-3; Drug: 619267-18-4; Drug: 619267-19-5; Drug: 619267-20-8; Drug: 619267-21-9; Drug: 619267-22-0; Drug: 619267-23-1; Drug: 619267-24-2; Drug: 619267-25-3; Drug: 619267-26-4; Drug: 678187-02-5; Drug: 678187-03-6; Drug: 678187-04-7; Drug: 678187-05-8; Drug: 678187-06-9; Drug: 678187-07-0; Drug: 678187-08-1; Drug: 678187-09-2; Drug: 678187-10-5; Drug: 678187-11-6; Drug: 678187-12-7; Drug: 678187-13-8; Drug: 678187-14-9. chemical inhibition with antimicrotubule agents leads to cell cycle arrest in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied.		References:	15027874
microtubule polymerization (GO:0046785)	decreased (PATO:0000468)	in vitro (MI:0492)		inferred from specific protein inhibition (ECO:0000020)	Leishmania donovani	79195
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 19044-89-4; Drug: 19044-94-1; Drug: 483279-44-3; Drug: 483279-45-4; Drug: 619267-13-9; Drug: 619267-14-0; Drug: 619267-15-1; Drug: 619267-16-2; Drug: 619267-17-3; Drug: 619267-18-4; Drug: 619267-19-5; Drug: 619267-20-8; Drug: 619267-21-9; Drug: 619267-22-0; Drug: 619267-23-1; Drug: 619267-24-2; Drug: 619267-25-3; Drug: 619267-26-4; Drug: 678187-02-5; Drug: 678187-03-6; Drug: 678187-04-7; Drug: 678187-05-8; Drug: 678187-06-9; Drug: 678187-07-0; Drug: 678187-08-1; Drug: 678187-09-2; Drug: 678187-10-5; Drug: 678187-11-6; Drug: 678187-12-7; Drug: 678187-13-8; Drug: 678187-14-9. chemical inhibition with antimicrotubule agents prevents mictotubule formation in vitro with purified tubulin; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied.		References:	15027874
growth (GO:0040007)	decreased time (PATO:0000716)	single cell organism (CARO:0000064)	amastigote (BTO:0000062)	inferred from bioassay (ECO:0000094)	Leishmania donovani	No drug identifiers listed for this gene.
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 902779-03-7; Drug: 908843-57-2; Drug: 908843-58-3; Drug: 908843-61-8; Drug: 908843-63-0; Drug: 908843-68-5; Drug: 908843-70-9; Drug: 908843-73-2; Drug: 908843-74-3; Drug: 908843-75-4; Drug: 908843-76-5; Drug: 908843-77-6; Drug: 908843-78-7; Drug: 908843-79-8; Drug: 908843-80-1; Drug: 908843-81-2; Drug: 908843-82-3; Drug: 908843-84-5. chemical inhibition with antimicrotubule agents leads to cell cycle arrest in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied.		References:	16675220
microtubule polymerization (GO:0046785)	decreased (PATO:0000468)	in vitro (MI:0492)		inferred from specific protein inhibition (ECO:0000020)	Leishmania donovani	No drug identifiers listed for this gene.
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 902779-03-7; Drug: 908843-57-2; Drug: 908843-58-3; Drug: 908843-61-8; Drug: 908843-63-0; Drug: 908843-68-5; Drug: 908843-70-9; Drug: 908843-73-2; Drug: 908843-74-3; Drug: 908843-75-4; Drug: 908843-76-5; Drug: 908843-77-6; Drug: 908843-78-7; Drug: 908843-79-8; Drug: 908843-80-1; Drug: 908843-81-2; Drug: 908843-82-3; Drug: 908843-84-5. chemical inhibition with antimicrotubule agents prevents mictotubule formation in vitro with purified tubulin; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied.		References:	16675220
microtubule polymerization (GO:0046785)	decreased (PATO:0000468)	in vitro (MI:0492)		inferred from specific protein inhibition (ECO:0000020)	Leishmania tarentolae	79195
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 483279-44-3; Drug: 619267-15-1. chemical inhibition with antimicrotubule agents prevents mictotubule formation in vitro with purified tubulin; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied.		References:	16753146
growth (GO:0040007)	decreased time (PATO:0000716)	single cell organism (CARO:0000064)	amastigote (BTO:0000062)	inferred from bioassay (ECO:0000094)	Leishmania mexicana	449723
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 209345-04-0; Drug: 58915-19-8; Drug: 209345-05-1; Drug: 218935-77-4; Drug: 268749-50-4. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied.		References:	16918078
growth (GO:0040007)	decreased time (PATO:0000716)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from bioassay (ECO:0000094)	Leishmania infantum	449723
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 209345-04-0; Drug: 58915-19-8; Drug: 209345-05-1; Drug: 218935-77-4; Drug: 268749-50-4. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied.		References:	16918078
growth (GO:0040007)	decreased time (PATO:0000716)	single cell organism (CARO:0000064)	promastigote (BTO:0001124)	inferred from bioassay (ECO:0000094)	Leishmania mexicana	449723
Annotator:	aaronjr@u.washington.edu.	Comment:	Drug: 209345-04-0; Drug: 58915-19-8; Drug: 209345-05-1; Drug: 218935-77-4; Drug: 268749-50-4. chemical inhibition with antimicrotubule agents leads to slow growth of Leishmania sp. in cell assay; unclear as to which topoisomerase I gene(s)/enzyme(s) was/were studied.		References:	16918078

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

64 chemical compounds are associated with this gene

Predicted associations

By orthology with druggable targets

Species	Known druggable target	Linked compounds	Reference
Leishmania major	beta tubulin	Compounds	References
Homo sapiens	melanocortin 1 receptor (alpha melanocyte stimulating hormone receptor)	Compounds	References
Leishmania major	beta tubulin	Compounds	References
Leishmania major	beta tubulin	Compounds	References
Leishmania major	beta tubulin	Compounds	References
Homo sapiens	tubulin, beta 6 class V	Compounds	References
Leishmania major	beta tubulin	Compounds	References
Leishmania donovani	Beta tubulin	Compounds	References
Homo sapiens	tubulin, beta 2B class IIb	Compounds	References
Leishmania major	beta tubulin	Compounds	References
Plasmodium falciparum	tubulin beta chain	Compounds	References
Homo sapiens	tubulin, beta 4B class IVb	Compounds	References
Leishmania major	beta tubulin	Compounds	References
Trypanosoma brucei	beta tubulin	Compounds	References
Leishmania major	beta tubulin	Compounds	References
Homo sapiens	tubulin, beta 2A class IIa	Compounds	References
Leishmania major	beta tubulin	Compounds	References
Bos taurus	Tubulin beta chain	Compounds	References
Leishmania major	beta tubulin	Compounds	References
Homo sapiens	tubulin, beta 8 class VIII	Compounds	References
Leishmania major	beta tubulin	Compounds	References
Leishmania major	beta tubulin	Compounds	References
Homo sapiens	tubulin, beta 1 class VI	Compounds	References
Leishmania major	beta tubulin	Compounds	References
Leishmania major	beta tubulin	Compounds	References
Homo sapiens	tubulin, beta 4A class IVa	Compounds	References

By sequence similarity to non orthologous druggable targets

Species	Target	Length	Identity	Alignment span	Linked Drugs	Reference
Bos taurus	Tubulin	85 aa	50.0%	80 aa	Compounds	References

Ranking Plot

Putative Drugs List

Raw	Global	Species
0.0507	0.2641	1
0.0919	0.5488	1
0.0771	0.6023	0.8449
0.0208	0.2549	1
0.2504	0.2918	0.2918
0.0366	0.4116	0.411
0.0234847	1	1
0.0852	0.3104	0.4014
0.0634	0.6501	0.5
0.169	0.3196	1
0.1286	0.3012	0.8086
0.2012	0.972	1
0.0262	0.3114	1
0.0453	0.5132	0.8237
0.3123	1	1
0.0274	1	1
0.1355	0.5558	1
0.0779	0.9931	1
0.1341	0.831	1
0.1511	0.2892	1
0.0811	0.4938	1
0.1035	0.3985	1
0.1528	0.2963	1
0.0166	0.2921	1
0.0086	0.4594	1
0.1828	0.5195	1
0.031	1	1
0.198	0.5535	1
0.0266	1	1
0.1412	0.4275	1
0.0502	1	1
0.1466	0.2511	1
0.1667	0.4936	1
0.1478	0.2526	1
0.0051	1	1
0.0017	1	0.5
0.0179	0.7609	1
0.0604	0.3576	1
0.004	1	1
0.0607	0.6181	0.6179
0.0298	1	1
0.0526	0.2904	1
0.0041	1	1
0.26	0.407	1
0.0334	0.3917	1
0.0417	0.4256	0.3585
0.0081	1	1
0.1354	0.2618	1
0.0123	0.3138	1
0.0351	1	0.5
0.03	0.3558	1
0.1068	0.6668	1
0.0556	0.3211	1
0.1667	1	1
0.1708	0.4968	1
0.0053	1	1
0.1058	0.3153	1
0.1365	0.2783	1
0.0299	0.8116	1
0.1321	0.4679	1
0.16	0.2591	1
0.0508	1	1
0.1114	0.7012	1
0.0345	0.663	1
0.0246	0.3156	1
0.0246	1	1
0.1713	0.4968	1
0.0274	0.2625	1
0.2504	0.2918	0.2918
0.0498	0.9712	1
0.119	0.3318	1
0.1959	0.5205	1
0.0286	0.3437	0.8725
0.0584	0.3403	1
0.003	1	0.5
0.0759	0.4488	0.5499
0.0081	0.274	0.5
0.1465	1	1
0.1012	0.5855	0.753
0.0137	0.4101	1
0.0027	0.5	0.5
0.08	1	1
0.0323	0.3607	1
0.0479	0.6585	1
0.0262	0.3114	1
0.0409	0.2722	0.2684
0.0031	0.8668	0.8662
0.0246	0.8122	1
0.0803	0.4937	1
0.0837	0.6783	1
0.1602	1	1
0.012	1	1
0.0278	1	1
0.1071	0.9095	1
0.128	0.7618	1
0.0352	0.3591	1
0.1977	1	1
0.1464	0.3322	1
0.024	1	1
0.1798	0.3258	1
0.0129	1	1
0.1466	0.297	1
0.0531	0.4202	1
0.3114	0.4071	1
0.0064	1	1
0.0027	0.5	0.5
0.0312	0.6247	1
0.0655	0.3494	1
0.0033	0.6334	1
0.1743	1	1
0.1836	0.4421	1
0.0636	0.2759	0.2758
0.1755	0.3219	1
0.1701	0.2903	1
0.0198	0.6641	0.5
0.0051	0.253	1
0.1586	0.4982	1
0.1365	0.2716	1
0.0783	0.4939	1
0.0067	0.5	0.5
0.0027	1	0.5
0.1634	0.3084	1
0.0486	1	1
0.016	0.5	0.5
0.2333	0.4633	1
0.0125527	1	1
0.0272	1	1
0.1097	0.4519	1
0.0132	1	1
0.0025	1	1
0.2812	1	1
0.2533	1	1
0.3114	0.4071	1
0.1637	0.3167	1
0.0129	1	1
0.0701	0.4454	1
0.196	0.8721	1
0.0166	1	1
0.0725	1	1
0.0344	0.3618	0.3546
0.1025	0.2664	1
0.0239	0.5537	1
0.1685	0.2763	0.8769
0.0584	1	1
0.0712	0.4817	1
0.0285	0.2825	1
0.0014	0.4677	0.4677
0.0795	0.3099	1
0.1647	0.3276	1
0.0017	1	0.5
0.0125	1	1
0.0087	0.4311	1
0.0225	0.5537	1
0.0314	1	1
0.1684	0.4948	1
0.1962	0.5449	1
0.0923	0.7174	1
0.1833	0.3262	1
0.041	0.8293	0.7094
0.1729	0.4918	1
0.1722	0.4949	1
0.0779	0.9931	1
0.0808	1	1
0.0744664	0.257377	1
0.0526	0.6274	1
0.1761	0.4996	1
0.0156	1	1
0.092	0.4646	1
0.4156	1	1
0.0174	0.312	1
0.0303	0.3743	1
0.0378	1	1
0.0196	0.3611	1
0.1165	0.3652	1
0.0111	0.3108	0.3086
0.0237	1	1
0.0965	0.6501	1
0.0309	1	1
0.1347	0.2705	1
0.1053	1	1
0.0184	0.5	0.5
0.1508	0.4583	1
0.0508	0.6181	0.6179
0.0252	0.5	0.5
0.0241	0.4638	1
0.1329	0.382	1
0.0197	0.395	1
0.386	0.3402	0.3402
0.1705	1	1
0.0183	1	1
0.0027	0.5	0.5
0.0783	0.4936	1
0.1687	0.5157	1
0.0484	0.3069	0.6057
0.0278	0.275	0.275
0.1279	1	1
0.0516	0.992	1
0.1876	0.5141	1
0.2441	0.3149	1
0.0027	0.5	0.5
0.0027	0.5	0.5
0.1922	0.5193	1
0.18	0.4993	1
0.0106	1	1
0.1647	0.3767	1
0.0017	1	0.5
0.1671	0.3162	1
0.0024	1	1
0.0233	0.5538	1
0.0025	0.3598	0.5
0.0233	1	1
0.0463	1	1
0.1922	0.5194	1
0.1271	1	1
0.0539	1	1
0.0176	1	1
0.2272	1	1
0.0347	0.4652	1
0.1468	0.2869	1
0.0473	1	1
0.1989	0.5269	1
0.0238	0.8171	1
0.0242	0.523	1
0.386	0.3402	0.3402
0.1086	0.682	1
0.1669	0.3252	1
0.1693	0.3254	1
0.1655	0.2739	1
0.0255	0.5031	1
0.1482	0.2908	1
0.0383	1	1
0.0259	1	1
0.0877	0.8002	1
0.0124	1	1
0.0162	0.8515	0.5
0.0654453	0.649599	0.670788
0.1138	1	1
0.1101	0.2599	1
0.006	1	1
0.0812	0.3254	0.4018
0.1848	0.5098	1
0.1378	0.2712	1
0.0837	0.6783	1
0.0529	0.407	1
0.1751	0.3978	0.9547
0.0876	0.3257	0.4022
0.1828	0.5091	1
0.1113	1	1
0.0143	0.5	0.5
0.0487	0.504	1
0.101296	1	1
0.0081	0.3074	0.3452
0.0322	0.5	0.5
0.3688	1	1
0.0062	1	0.5
0.0771	0.6023	0.8449
0.0053	0.5	0.5
0.0027	0.5	0.5
0.012	0.2759	1
0.0241	1	1
0.1608	0.4981	1
0.0521	0.5955	1
0.1726	0.3219	1
0.0846	1	1
0.1479	0.3509	1
0.0024	0.4659	1
0.0579	0.6644	1
0.0773	0.3254	0.402
0.1455	0.2511	1
0.1346	0.4201	1
0.1944	0.5474	1
0.0967	0.3896	1
0.0586	0.3691	1
0.0266	0.3683	0.853
0.093	0.5691	1
0.0841	0.4637	0.9117
0.1671	1	1
0.152	0.2954	1
0.0236	0.386	1
0.0074	1	1
0.1343	0.2717	1
0.0468	0.5928	0.5
0.0483	1	1
0.0042	0.6768	0
0.0233	1	1
0.03	0.3598	1
0.084	0.3512	1
0.0231	1	1
0.4202	1	1
0.0027	0.5	0.5
0.0159	0.3332	0.5
0.0768	0.4221	0.5795
0.1052	0.6538	1
0.0625	1	1
0.141	0.5661	1
0.0729	0.4578	1
0.386	0.3402	0.3402
0.0453	0.6685	1
0.0265	1	1
0.1828	0.5195	1
0.0753	0.5226	1
0.0852	0.5177	1
0.048	0.3051	1
0.0322	0.6957	1
0.1343	0.2728	1
0.1788	0.4987	1
0.0124	1	1
0.003	1	1
0.0056	1	0.5
0.0465	0.4598	1
0.0557	1	1
0.0338	1	1
0.2383	1	1
0.205	1	1
0.0027	0.5	0.5
0.0239	0.4792	1
0.386	0.3402	0.3402
0.0031	0.3316	0
0.141	0.5661	1
0.0302	0.2908	1
0.1554	0.2846	0.5845
0.2504	0.2918	0.2918
0.0251	0.4788	1
0.1833	0.6121	1
0.0141	0.4569	1
0.0069	1	1
0.1884	0.2613	1
0.058	0.2865	1
0.0582	1	1
0.0027	0.5	0.5
0.0312	0.6242	1
0.1062	0.4389	1
0.0526	1	1
0.6091	0.322	1
0.0347	0.4114	0.9229
0.0027	0.5	0.5
0.08	1	1
0.002	0.6881	0.6873
0.0532	0.5559	0.6205
0.1664	0.5051	1
0.0588	0.9295	0.9295
0.1819	0.3231	1
0.1052	1	1
0.0056	1	1
0.1903	0.5489	1
0.1827	0.3993	1
0.1706	0.4929	1
0.0569	0.2664	1
0.0936	0.2819	1
0.141	0.5661	1
0.1675	0.3307	1
0.029	1	1
0.0235	1	1
0.0306	0.3596	1
0.0616	0.263	1
0.05	1	1
0.0341	0.3745	1
0.0269	0.3418	1
0.0481	1	1
0.0183	0.371	1
0.0301	1	1
0.1885	0.6031	1
0.538	0.48	1
0.1776	0.4993	1
0.1055	0.6529	1
0.1912	0.5078	1
0.1138	1	1
0.0237	0.4678	1
0.135	0.2725	1
0.0366	0.3607	1
0.1637	0.3173	1
0.0344	0.3713	1
0.017	1	1
0.2115	1	1
0.15907	1	1
0.1591	0.4661	1
0.0225	0.3703	1
0.3002	0.9694	1
0.0775	0.4123	1
0.0068	1	1
0.0027	0.5	0.5
0.0577283	1	1
0.1639	0.3178	1
0.3303	1	1
0.1169	0.9587	1
0.0386	0.3397	0.555
0.0173	0.7288	1
0.2187	0.2819	1
0.1683	0.3173	1
0.0027	0.5	0.5
0.1831	0.5555	1
0.0616	0.263	1
0.1613	0.6669	1
0.1524	0.2965	1
0.0133	0.5	0.5
0.1635	0.4985	1
0.024	1	1
0.0636	0.437	1
0.1834	0.5035	1
0.0278	1	1
0.1721	0.4919	1
0.0549	1	1
0.028	0.3971	1
0.2117	0.3406	0.9567
0.0243	0.3904	1
0.0051	0.5589	0
0.0504	0.9418	1
0.1052	1	1
0.004	1	1
0.0528	0.6204	0.6195
0.0028	1	1
0.1905	0.508	1
0.0232966	1	1
0.0787	0.3283	1
0.1619	0.3173	1
0.1115	1	1
0.0328267	1	0.5
0.1884	0.2613	1
0.0835	1	1
0.1399	0.6411	1
0.0164	0.6827	0.5
0.1757	0.5005	1
0.0897	0.3314	1
0.1147	0.2974	1
0.0313	0.3655	1
0.2383	1	1
0.0795	0.4909	1
0.0524	0.3329	0.7993
0.0247	1	1
0.0529	0.3782	1
0.1114	1	1
0.0545	0.6204	0.6195
0.0983	0.6925	1
0.321	0.8193	1
0.0511	1	1
0.2267	0.3093	1
0.1126	0.6329	1
0.2299	0.3999	1
0.048	0.3051	1
0.0287	0.3626	1
0.0041	1	1
0.0439	0.2508	0.4046
0.029	0.3085	1
0.1714	0.3213	1
0.1745	0.4949	1
0.1524	0.4278	1
0.0603	0.6181	0.6179
0.0631	0.7608	1
0.0225	0.591	1
0.0435	1	1
0.0026	1	0.5
0.0156	0.9315	1
0.0855	1	1
0.0342	0.9826	1
0.0479	1	1
0.2115	1	1
0.0316	0.3603	1
0.0584	0.7152	1
0.0114	0.5156	0.5761
0.0027	0.5	0.5
0.0079	0.5341	1
0.1211	1	1
0.0215	0.416	0.416
0.0652	1	1
0.0344	1	1
0.1508	0.4583	1
0.0804	0.8736	1
0.1694	0.3066	1
0.004	1	1
0.0027	0.5	0.5
0.1272	0.429	1
0.0027	0.5	0.5
0.1652	0.3153	1
0.193	0.296	0.9831
0.1558	1	1
0.0619	0.6181	0.6179
0.0274	1	1
0.0593	0.3464	1
0.15992	1	1
0.0693	1	1
0.0111	0.3444	0.5
0.1508	0.2917	0.2917
0.1057	0.5894	1
0.0584	1	1
0.0233383	0.361958	1
0.0513	1	1
0.0231	0.748	1
0.0278	0.3345	1
0.1807	0.4962	1
0.0464	0.2779	1
0.1305	1	1
0.3409	0.8743	1
0.1846	0.5052	1
0.0243	1	1
0.1623	0.507	1
0.2161	0.3905	1
0.6091	0.322	1
0.0235	1	1
0.0539	0.6199	0.6195
0.0238	1	1
0.0455	0.2907	1
0.0053	0.2793	1
0.0716	0.42	1
0.0027	0.5	0.5
0.1404	0.3341	1
0.0512	0.6199	0.6195
0.0133	0.5	0.5
0.0193	1	1
0.0095	1	1
0.00207208	1	1
0.0796	0.4909	1
0.1673	0.3161	1
0.0257	0.2972	1
0.1948	0.3006	1
0.1202	0.5953	1
0.2534	1	1
0.1801	0.325	1
0.0257	1	0.5
0.1721	0.4919	1
0.070476	1	1
0.0104	1	1
0.0756	0.4655	0.5704
0.0085	0.8488	0.8488
0.021	0.2543	1
0.0625	0.4036	1
0.0100856	1	1
0.327	1	1
0.0024	0.5	0.5
0.0087	0.3772	1
0.234	1	1
0.1673	0.4948	1
0.0389	0.6243	1
0.1471	0.4408	1
0.1177	1	1
0.0346	0.4249	1
0.1028	1	1
0.1353	0.5641	1
0.0054	1	0.5
0.0027	0.5	0.5
0.1708	1	1
0.0698	0.3937	1
0.2333	0.4633	1
0.036	0.2966	0.7268
0.0149	0.3224	0.3974
0.1862	0.2793	1
0.0249	0.3006	1
0.1808	0.3257	1
0.2441	0.361	1
0.1557	0.2849	1
0.0466	0.3538	1
0.0503	0.9716	1
0.386	0.3402	0.3402
0.0237	0.2792	1
0.0608	0.2842	1
0.1779	0.3848	1
0.0247	0.2907	1
0.0081	1	1
0.0471	1	1
0.0024	0.9688	0.5
0.1283	0.8272	1
0.0396	0.4514	1
0.0625	0.7492	1
0.0073	1	1
0.0345	0.3617	1
0.0237	0.5813	1
0.6091	0.322	1
0.1263	0.7293	1
0.1764	0.3188	1
0.0399	1	0.5
0.1895	0.5163	1
0.6091	0.322	1
0.1795	0.3159	1
0.0641	0.3105	0.6456
0.2806	0.6258	1
0.0852	0.3515	1
0.0705	0.8135	1
0.0162	1	1
0.0233	1	1
0.05	1	1
0.1309	0.3801	1
0.1422	0.3223	1
0.1252	1	1
0.1253	0.3668	1
0.0559	0.7432	1
0.055	0.2743	1
0.1548	0.2744	1
0.03	0.3597	1
0.1812	0.646	1
0.1738	0.4924	1
0.0027	0.5	0.5
0.1156	0.2844	1
0.0241	1	1
0.0917	0.5508	1
0.1455	0.2511	1
0.1801	0.5095	1
0.0546	1	1
0.0027	0.5	0.5
0.174	0.4847	1
0.1403	0.8041	1
0.1231	0.3619	1
0.0124	0.4768	0.4768
0.15	0.438	1
0.0258	1	1
0.0057	1	0.5
0.0625	1	1
0.1173	0.3488	1
0.1798	0.3252	0.7745
0.0264	0.3323	0.3413
0.0474	0.2902	1
0.1844	0.505	1
0.0729	0.999	1
0.1106	0.4519	1
0.2081	1	1
0.1043	0.7316	1
0.0053	0.3237	1
0.0808	0.326	1
0.134	0.38	1
0.1038	0.6491	1
0.1992	0.3546	1
0.082	0.333	1
0.1628	0.3172	1
0.1155	1	1
0.0532	0.6341	1
0.1047	0.4376	1
0.1046	0.6549	1
0.0211	1	1
0.0051	1	1
0.1508	0.4583	1
0.1556	0.55	1
0.0027	0.5	0.5
0.1731	0.643	1
0.1353	0.8581	0.5
0.0837	1	1
0.1387	0.2725	1
0.252	0.2603	0.2602
0.003	0.9909	0.5
0.26	0.407	1
0.0075	0.7534	0.5
0.003	1	1
0.046	1	1
0.409	1	1
0.1715	0.3219	1
0.1477	0.4301	1
0.0228	0.4305	1
0.1404	0.3341	1
0.0181	0.8454	1
0.2369	0.4715	1
0.3995	1	1
0.0727	1	1
0.1072	0.4384	0.9672
0.0062	0.6619	0.5
0.1652	0.316	1
0.1088	0.683	1
0.0027	0.5	0.5
0.0801	0.4911	1
0.0091	0.4117	0
0.0405	0.623	0.6227
0.0305	1	1
0.089	0.2546	1
0.1756	0.3218	1
0.0229	0.4533	1
0.0917	0.5508	1
0.1558	1	1
0.03	0.3605	1
0.0481	1	1
0.0806	0.589	1
0.0329	0.377	1
0.0753	0.5226	1
0.1508	0.4583	1
0.0648	1	1
0.0569	0.9459	1
0.148	0.4154	1
0.047	0.4159	0.4196
0.0248	0.2718	0.5843
0.0936	0.2819	1
0.026	1	1
0.1647	0.2995	1
0.063	0.3736	0.8766
0.0472	0.6584	1
0.049	0.9596	1
0.0235	0.3604	1
0.0324	0.3592	0.7346
0.0384	0.2633	1
0.003	0.653	1
0.0794	0.5208	1
0.0102	0.4636	1
0.1015	0.735	1
0.1939	1	1
0.0458	0.6174	1
0.3114	0.4071	1
0.3114	0.4071	1
0.0027	0.5	0.5
0.2035	1	1
0.0632	0.8259	1
0.0533	0.4881	1
0.0754	0.5684	1
0.0253	0.4156	0.5
0.3096	0.7871	1
0.0894	0.6875	1
0.0487	1	1
0.0239	1	1
0.0256	0.5177	1
0.1581	0.3171	1
0.045	1	1
0.1502	0.3435	1
0.0051	0.4862	0.5
0.138	0.2688	1
0.0235	0.3604	1
0.024	0.5538	1
0.2091	0.3644	1
0.0934	0.3312	1
0.0121	0.2598	0.5
0.013	0.4281	1
0.0777	0.582	1
0.0269	0.4862	1
0.1588	0.3067	1
0.0077	1	1
0.0632	1	1
0.0241	0.5157	0.3974
0.1808	0.3257	1
0.0027	0.5	0.5
0.0485	0.5231	1
0.1068	1	1
0.0338	1	1
0.0609	0.6408	1
0.0811	0.4938	1
0.1343	0.7529	1
0.176	0.2542	1
0.0259	0.3135	1
0.049	0.4	1
0.0697	0.4193	1
0.0376	0.7275	1
0.1347	0.2748	1
0.0769	0.6178	1
0.0049	0.5	0.5
0.193	0.296	0.9831
0.064	1	1
0.0144	0.5	0.5
0.0027	0.5	0.5
0.1784	0.2845	1
0.0117	1	1
0.0625	0.4036	1
0.1379	0.8166	1
0.1031	1	1
0.135	0.2704	1
0.0709	1	1
0.0885	1	1
0.2504	0.2918	0.2918
0.0027	0.5	0.5
0.0124	1	1
0.1644	0.4949	1
0.0054	1	1
0.056	0.3721	1
0.0025	0.2578	0.2693
0.1068	1	1
0.2619	1	1
0.137969	0.540821	1
0.1145	1	1
0.1466	0.2511	1
0.1764	0.3213	1
0.0541	1	1
0.1668	0.4235	1
0.141	0.5661	1
0.4021	1	1
0.0027	0.4648	0.5
0.0814	0.5	0.5
0.0237	0.5538	1
0.0278	1	1
0.5229	0.3799	1
0.0597	0.3109	1
0.0958	1	1
0.0086	0.376	1
0.0263	0.5982	1
0.0278	1	1
0.0208	0.2549	1
0.0047	1	1
0.0503	0.4297	1
0.0027	0.5	0.5
0.0329	0.3596	0.7602
0.1938	0.5475	1
0.00267279	1	1
0.2137	1	1
0.0031	0.3861	0
0.6091	0.322	1
0.0052	0.4322	1
0.1798	0.3231	1
0.135	0.273	1
0.1752	0.4866	1
0.1334	0.2733	1
0.2333	0.4633	1
0.0974	0.4366	1
0.0542	0.3265	1
0.0247	0.4457	1
0.0294	1	1
0.1372	0.5635	1
0.0094	0.2612	0.5
0.1629	0.3211	1
0.0556	0.3204	1
0.1743	0.3202	1
0.0027	0.5	0.5
0.1112	0.2896	0.5948
0.092	0.4646	1
0.0056	1	1
0.0935	0.6924	1
0.055	0.2743	1
0.0026	0.5317	1
0.0045	0.5	0.5
0.1306	0.2505	1
0.005	1	1
0.1124	0.3977	1
0.1748	0.326	1
0.005	1	1
0.1552	0.2519	1
0.2388	0.3213	1
0.1581	0.4905	1
0.0698	1	1
0.0056	0.2955	1
0.0437	0.2891	1
0.0597	1	1
0.1359	0.2716	1
0.1655	0.317	1
0.6091	0.322	1
0.0128	1	1
0.1193	0.3142	1
0.0471	0.4923	1
0.0953	1	1
0.095	0.3255	0.4022
0.1253	0.3668	1
0.1349	0.2714	1
0.046	0.3517	1
0.1684	0.4948	1
0.1508	0.4583	1