Detailed view for Bm1_45455

Basic information

TDR Targets ID: 241424
Brugia malayi, prolyl 4-hydroxylase
Source Database / ID:  GenBank

pI: 5.9866 | Length (AA): 541 | MW (Da): 62193 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF08336   Prolyl 4-Hydroxylase alpha-subunit, N-terminal region
PF13640   2OG-Fe(II) oxygenase superfamily

Gene Ontology

Mouse over links to read term descriptions.
GO:0016705   oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen  
GO:0016702   oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen  
GO:0005783   endoplasmic reticulum  
GO:0004656   procollagen-proline 4-dioxygenase activity  
GO:0031418   L-ascorbic acid binding  
GO:0016491   oxidoreductase activity  
GO:0005515   protein binding  
GO:0005506   iron ion binding  
GO:0005488   binding  
GO:0055114   oxidation reduction  

Metabolic Pathways

Arginine and proline metabolism (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
20 258 4bt9 (A) 5 238 38.00 0 1 0.910975 -0.2
131 278 3vtx (A) 76 216 13.00 0.44 0.45 0.414567 -0.47
163 248 5hrz (A) 34 111 29.00 0.24 0.95 0.512165 -0.79
316 517 3itq (A) 21 213 28.00 0 1 0.658783 -0.4
321 518 2jig (A) 38 248 33.00 0 1 0.762989 -0.68
321 517 2v4a (A) 38 247 34.00 0 1 0.43954 0

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_128524)

Species Accession Gene Product
Arabidopsis thaliana AT5G66060   2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Arabidopsis thaliana AT3G28480   oxoglutarate/iron-dependent oxygenase
Arabidopsis thaliana AT2G17720   prolyl 4-hydroxylase 5
Arabidopsis thaliana AT3G28490   Oxoglutarate/iron-dependent oxygenase
Arabidopsis thaliana AT1G20270   2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Arabidopsis thaliana AT4G35810   oxidoreductase, 2OG-Fe(II) oxygenase family protein
Brugia malayi Bm1_45455   prolyl 4-hydroxylase
Caenorhabditis elegans CELE_Y47D3B.10   Protein DPY-18
Caenorhabditis elegans CELE_F35G2.4   Protein PHY-2
Dictyostelium discoideum DDB_G0275385   hypothetical protein
Drosophila melanogaster Dmel_CG31022   prolyl-4-hydroxylase-alpha EFB
Drosophila melanogaster Dmel_CG9698   CG9698 gene product from transcript CG9698-RA
Echinococcus granulosus EgrG_000670500   prolyl 4 hydroxylase subunit alpha 1
Echinococcus multilocularis EmuJ_000670500   prolyl 4 hydroxylase subunit alpha 1
Homo sapiens ENSG00000072682   prolyl 4-hydroxylase, alpha polypeptide II
Homo sapiens ENSG00000122884   prolyl 4-hydroxylase, alpha polypeptide I
Loa Loa (eye worm) LOAG_06927   prolyl 4-hydroxylase 2
Mus musculus ENSMUSG00000019916   procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha 1 polypeptide
Mus musculus ENSMUSG00000018906   procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha II polypeptide
Oryza sativa 4349012   Os10g0497800
Oryza sativa 4334477   Os03g0803500
Oryza sativa 9270970   Os10g0415128
Oryza sativa 4342642   Os07g0194500
Oryza sativa 4348615   Os10g0413500
Schistosoma japonicum Sjp_0302180   ko:K00472 prolyl 4-hydroxylase [EC1.14.11.2], putative
Schistosoma mansoni Smp_068280   prolyl 4-hydroxylase alpha subunit 1
Schmidtea mediterranea mk4.002371.08  
Schmidtea mediterranea mk4.000450.06  

Essentiality

Bm1_45455 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
CELE_Y47D3B.10 Caenorhabditis elegans embryonic lethal wormbase
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Rattus norvegicus Prolyl 4-hydroxylase alpha-1 subunit Compounds References
Homo sapiens prolyl 4-hydroxylase, alpha polypeptide I Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0185 0.5693 1
0.0225 0.2563 0.4384
0.0151288 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Bm1_45455 (Brugia malayi), prolyl 4-hydroxylase
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