Detailed view for LmjF.34.4460

Basic information

TDR Targets ID: 24761
Leishmania major, 20s proteasome beta 7 subunit, (putative)
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.7888 | Length (AA): 220 | MW (Da): 24694 | Paralog Number: 6

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00227   Proteasome subunit

Gene Ontology

Mouse over links to read term descriptions.
GO:0005839   proteasome core complex  
GO:0004298   threonine endopeptidase activity  
GO:0004175   endopeptidase activity  
GO:0051603   proteolysis involved in cellular protein catabolic process  

Metabolic Pathways

Proteasome (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 210 5le5 (M) 5 213 39.00 0 1 1.52495 -0.87
3 195 1ryp (M) 0 204 23.00 0.0002 1 1.13507 -0.45

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_127562)

Species Accession Gene Product
Arabidopsis thaliana AT1G56450   20S proteasome beta subunit G1
Babesia bovis BBOV_IV002060   proteasome subunit beta type 4
Brugia malayi Bm1_48355   Proteasome A-type and B-type family protein
Candida albicans CaO19.4230   endopeptidase subunit of proteasome
Candida albicans CaO19.11705   endopeptidase subunit of proteasome
Caenorhabditis elegans CELE_F39H11.5   Protein PBS-7
Cryptosporidium hominis Chro.50050   beta tubulin
Cryptosporidium parvum cgd5_3220   proteasome subunit beta7; NTN hydrolase fold
Dictyostelium discoideum DDB_G0273163   20S proteasome subunit beta-4
Dictyostelium discoideum DDB_G0273909   20S proteasome subunit alpha-4
Drosophila melanogaster Dmel_CG12000   Proteasome beta7 subunit
Echinococcus granulosus EgrG_001150600   proteasome prosome macropain subunit beta type
Entamoeba histolytica EHI_137970   proteasome subunit beta Type 4 precursor, putative
Echinococcus multilocularis EmuJ_001150600   proteasome (prosome macropain) subunit beta type
Giardia lamblia GL50803_1995   Proteasome subunit beta type 4 precursor
Homo sapiens ENSG00000159377   proteasome (prosome, macropain) subunit, beta type, 4
Leishmania braziliensis LbrM.20.3950   20s proteasome beta 7 subunit, (putative)
Leishmania braziliensis LbrM.20.3980   20s proteasome beta 7 subunit, (putative)
Leishmania infantum LinJ.34.4280   hypothetical protein, conserved
Leishmania infantum LinJ.34.4390   20s proteasome beta 7 subunit, (putative)
Leishmania major LmjF.34.4400   20s proteasome beta 7 subunit, (putative)
Leishmania major LmjF.34.4430   20s proteasome beta 7 subunit, (putative)
Leishmania major LmjF.34.4460   20s proteasome beta 7 subunit, (putative)
Leishmania major LmjF.34.4520   proteasome beta 7 subunit, putative
Leishmania major LmjF.34.4370   20s proteasome beta 7 subunit, (putative)
Leishmania major LmjF.34.4340   20s proteasome beta 7 subunit, (putative)
Leishmania major LmjF.34.4490   20s proteasome beta 7 subunit, (putative)
Leishmania mexicana LmxM.33.4340   20s proteasome beta 7 subunit, (putative)
Loa Loa (eye worm) LOAG_09447   proteasome A-type and B-type family protein
Mus musculus ENSMUSG00000005779   proteasome (prosome, macropain) subunit, beta type 4
Neospora caninum NCLIV_019480   proteasome A-type and B-type domain-containing protein, putative
Oryza sativa 4347573   Os09g0515200
Plasmodium berghei PBANKA_1226500   proteasome subunit beta type-4, putative
Plasmodium falciparum PF3D7_0803800   proteasome subunit beta type-4
Plasmodium knowlesi PKNH_0115100   proteasome subunit beta type-4, putative
Plasmodium vivax PVX_093555   proteasome subunit beta type-4, putative
Plasmodium yoelii PY00806   proteasome beta-subunit
Saccharomyces cerevisiae YFR050C   proteasome core particle subunit beta 7
Schistosoma japonicum Sjp_0068310   ko:K02736 20S proteasome subunit beta 7, putative
Schistosoma mansoni Smp_056500   proteasome subunit beta 4 (T01 family)
Schmidtea mediterranea mk4.000293.03   Proteasome subunit beta type-4
Trypanosoma brucei gambiense Tbg972.4.170   proteasome beta 7 subunit, putative
Trypanosoma brucei Tb927.4.430   proteasome beta 7 subunit
Trypanosoma congolense TcIL3000_4_120   proteasome beta 7 subunit
Trypanosoma cruzi TcCLB.510689.30   proteasome beta 7 subunit, putative
Toxoplasma gondii TGME49_280710   20S proteasome subunit beta 7, putative
Theileria parva TP03_0446   20S proteasome beta 7 subunit, putative
Trichomonas vaginalis TVAG_290960   Family T1, proteasome beta subunit, threonine peptidase

Essentiality

LmjF.34.4460 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.430 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.4.430 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.4.430 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.4.430 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F39H11.5 Caenorhabditis elegans embryonic lethal wormbase
CELE_F39H11.5 Caenorhabditis elegans larval arrest wormbase
CELE_F39H11.5 Caenorhabditis elegans larval lethal wormbase
CELE_F39H11.5 Caenorhabditis elegans slow growth wormbase
CELE_F39H11.5 Caenorhabditis elegans sterile wormbase
YFR050C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1226500 Plasmodium berghei Slow plasmo
TGME49_280710 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens proteasome (prosome, macropain) subunit, beta type, 4 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0128 0.3103 0.4555
0.0246 0.3118 0.4585
0.0132 0.3115 0.4568
0.0132 0.313 0.4585
0.0132 0.3115 0.4568
0.0115 0.3329 0.3515
0.0132 0.3115 0.4568
0.023 0.313 0.4585
0.0244 0.3254 0.4698
0.0221 0.313 0.4585
0.0107 0.3115 0.4568
0.011 0.2583 0.4568
0.0132 0.313 0.4585
0.0122 0.3115 0.4568
0.0238 0.313 0.4585
0.0118 0.3326 0.3535
0.025 0.3116 0.4585
0.0132 0.3115 0.4568

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.34.4460 (Leishmania major), 20s proteasome beta 7 subunit, (putative)
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