Detailed view for LmjF.04.0960

Basic information

TDR Targets ID: 25363
Leishmania major, adenylate kinase, putative

Source Database / ID:  TriTrypDB  GeneDB

pI: 6.1276 | Length (AA): 211 | MW (Da): 23397 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00406   Adenylate kinase

Gene Ontology

Mouse over links to read term descriptions.
GO:0019205   nucleobase, nucleoside, nucleotide kinase activity  
GO:0006139   nucleobase, nucleoside, nucleotide and nucleic acid metabolic process  
GO:0005524   ATP binding  

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
8 205 1ukz () 9 204 41.00 0 1 1.53 -1.4
13 204 2bwj (A) 6 196 36.00 0 1 1.45 -1.55
9 207 3umf (A) 4 197 36.00 0 1 1.43933 -1.3
19 203 1ukz (A) 17 202 43.00 0 1 1.52098 -1.65
20 205 1qf9 (A) 8 193 44.00 0 1 1.51852 -1.46
22 200 1aky (A) 8 216 26.00 0 1 1.11254 -0.98

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_126886)

Species Accession Gene Product
Arabidopsis thaliana AT3G60180   uridylate kinase-like protein
Arabidopsis thaliana AT5G26667   UMP-CMP kinase
Babesia bovis BBOV_II007680   UMP-CMP kinase, putative
Brugia malayi Bm1_14420   UMP-CMP kinase family protein
Brugia malayi Bm1_13790   adenylate kinase isoenzyme 1
Candida albicans CaO19.12662   likely uridine kinase similar to S. cerevisiae URA6 (YKL024C)
Candida albicans CaO19.5195   likely uridine kinase similar to S. cerevisiae URA6 (YKL024C)
Caenorhabditis elegans CELE_F40F8.1   Protein F40F8.1
Caenorhabditis elegans CELE_C29F7.3   Protein C29F7.3
Cryptosporidium parvum cgd1_3140   P-loop nucleotide (UMP) kinase
Dictyostelium discoideum DDB_G0287495   UMP-CMP kinase
Drosophila melanogaster Dmel_CG17146   Adenylate kinase-1
Drosophila melanogaster Dmel_CG6092   CG6092 gene product from transcript CG6092-RA
Echinococcus granulosus EgrG_001107800   UMP CMP kinase
Echinococcus granulosus EgrG_000114000   adenylate kinase
Entamoeba histolytica EHI_044740   UMP-CMP kinase, putative
Entamoeba histolytica EHI_143580   UMP-CMP kinase, putative
Echinococcus multilocularis EmuJ_000114000   adenylate kinase
Echinococcus multilocularis EmuJ_001107800   UMP CMP kinase
Giardia lamblia GL50803_90402   Adenylate kinase
Giardia lamblia GL50803_4849   Adenylate kinase
Homo sapiens ENSG00000106992   adenylate kinase 1
Homo sapiens ENSG00000162368   cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
Leishmania braziliensis LbrM.04.0960   adenylate kinase, putative
Leishmania donovani LdBPK_040960.1   adenylate kinase, putative
Leishmania infantum LinJ.04.0960   adenylate kinase, putative
Leishmania major LmjF.04.0960   adenylate kinase, putative
Leishmania mexicana LmxM.04.0960   adenylate kinase, putative
Loa Loa (eye worm) LOAG_08791   UMP-CMP kinase
Loa Loa (eye worm) LOAG_13717   adenylate kinase 1
Mus musculus ENSMUSG00000026817   adenylate kinase 1
Mus musculus 66588   cytidine monophosphate (UMP-CMP) kinase 1
Neospora caninum NCLIV_028510   Uridylate kinase Ura6, related
Oryza sativa 4330910   Os02g0778400
Oryza sativa 9267059   Os06g0203500
Oryza sativa 4343960   Os07g0624700
Oryza sativa 4339882   Os06g0109600
Onchocerca volvulus OVOC1890   Adenylate kinase isoenzyme 1 homolog
Plasmodium berghei PBANKA_0202300   UMP-CMP kinase, putative
Plasmodium falciparum PF3D7_0111500   UMP-CMP kinase, putative
Plasmodium knowlesi PKNH_0202100   UMP-CMP kinase, putative
Plasmodium vivax PVX_081230   UMP-CMP kinase, putative
Plasmodium yoelii PY05596   ump-cmp kinase
Saccharomyces cerevisiae YKL024C   bifunctional uridylate/adenylate kinase
Schistosoma japonicum Sjp_0212050   ko:K00939 adenylate kinase [EC2.7.4.3], putative
Schistosoma mansoni Smp_071390   adenylate kinase
Schistosoma mansoni Smp_043620   cytidylate kinase
Schmidtea mediterranea mk4.014277.00   Putative adenylate kinase
Schmidtea mediterranea mk4.032381.00   Putative adenylate kinase
Schmidtea mediterranea mk4.000418.12  
Schmidtea mediterranea mk4.001366.01   Putative adenylate kinase
Schmidtea mediterranea mk4.013859.00   Putative adenylate kinase
Schmidtea mediterranea mk4.007490.00   UMP-CMP kinase
Schmidtea mediterranea mk4.000892.04   Putative adenylate kinase
Schmidtea mediterranea mk4.002057.01   UMP-CMP kinase
Schmidtea mediterranea mk4.000459.04   Putative adenylate kinase
Schmidtea mediterranea mk4.002231.02   Putative adenylate kinase
Schmidtea mediterranea mk4.018288.00   Putative adenylate kinase
Schmidtea mediterranea mk4.003938.03   Putative adenylate kinase
Schmidtea mediterranea mk4.023284.00   Putative adenylate kinase
Schmidtea mediterranea mk4.001204.01   Putative adenylate kinase
Schmidtea mediterranea mk4.038042.00  
Schmidtea mediterranea mk4.000051.23  
Schmidtea mediterranea mk4.026582.01   Putative adenylate kinase
Schmidtea mediterranea mk4.004672.00   UMP-CMP kinase
Schmidtea mediterranea mk4.018397.01   Putative adenylate kinase
Schmidtea mediterranea mk4.000037.12   Putative adenylate kinase
Schmidtea mediterranea mk4.064529.00   Putative adenylate kinase
Schmidtea mediterranea mk4.022072.00   Putative adenylate kinase
Schmidtea mediterranea mk4.001038.01   Putative adenylate kinase
Schmidtea mediterranea mk4.018067.00   Putative adenylate kinase
Schmidtea mediterranea mk4.005380.01   Putative adenylate kinase
Schmidtea mediterranea mk4.039209.00   Putative adenylate kinase
Schmidtea mediterranea mk4.004965.01   Putative adenylate kinase
Schmidtea mediterranea mk4.000313.05   Putative adenylate kinase
Schmidtea mediterranea mk4.039354.00   Putative adenylate kinase
Schmidtea mediterranea mk4.006642.00   Putative adenylate kinase
Schmidtea mediterranea mk4.021188.01   Putative adenylate kinase
Schmidtea mediterranea mk4.031525.00   Putative adenylate kinase
Schmidtea mediterranea mk4.038348.00   Putative adenylate kinase
Schmidtea mediterranea mk4.000056.08   Putative adenylate kinase
Schmidtea mediterranea mk4.002660.06   Putative adenylate kinase
Schmidtea mediterranea mk4.004240.00   Putative adenylate kinase
Schmidtea mediterranea mk4.001272.01   Putative adenylate kinase
Schmidtea mediterranea mk4.018288.01   Putative adenylate kinase
Schmidtea mediterranea mk4.027142.00   Putative adenylate kinase
Schmidtea mediterranea mk4.002715.01   UMP-CMP kinase
Schmidtea mediterranea mk4.001921.02   Putative adenylate kinase
Trypanosoma brucei gambiense Tbg972.9.4610   adenylate kinase, putative
Trypanosoma brucei Tb927.9.8450   UMP/CMP kinase
Trypanosoma congolense TcIL3000_9_2920   adenylate kinase, putative
Trypanosoma cruzi TcCLB.510575.180   adenylate kinase, putative
Trypanosoma cruzi TcCLB.510243.50   adenylate kinase, putative
Toxoplasma gondii TGME49_257740   UMP-CMP kinase
Theileria parva TP02_0690   UMP-CMP kinase, putative
Trichomonas vaginalis TVAG_273750   adenylate kinase, putative
Trichomonas vaginalis TVAG_160390   uridylate kinase, putative
Trichomonas vaginalis TVAG_179660   adenylate kinase isoenzyme, putative
Trichomonas vaginalis TVAG_493000   adenylate kinase 1, putative
Trichomonas vaginalis TVAG_070830   cytidylate kinase, putative
Trichomonas vaginalis TVAG_130730   adenylate kinase, putative
Trichomonas vaginalis TVAG_247200   adenylate kinase, putative

Essentiality

LmjF.04.0960 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.0350 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb09.211.0350 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb09.211.0350 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb09.211.0350 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F40F8.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_F40F8.1 Caenorhabditis elegans larval arrest wormbase
YKL024C Saccharomyces cerevisiae inviable yeastgenome
TGME49_257740 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5


Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Myokinase (2.7.4.3 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Leishmania donovani ( 1 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    LmjF.04.0960 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Leishmania donovani ( 1 )

Bibliographic References

2 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier LmjF.04.0960 (Leishmania major), adenylate kinase, putative
Title for this comment
Comment