Detailed view for ML0887c

Basic information

TDR Targets ID: 256284
Mycobacterium leprae, Probable long-chain-fatty-acid-CoA ligase FadD15 (FATTY-ACID-CoA SYNTHETASE) (FATTY-ACID-CoA SYNTHASE)
Source Database / ID:  Leproma 

pI: 6.2009 | Length (AA): 600 | MW (Da): 64463 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00501   AMP-binding enzyme

Gene Ontology

Mouse over links to read term descriptions.
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 595 2vsq (A) 447 964 23.00 0 1 0.9113 1.31
7 595 3pbk (A) 14 567 21.00 0 1 1.09397 0.7
12 520 1ult (A) 14 501 25.00 0 1 0.952633 0.28
56 200 1b9h (A) 64 199 23.00 0.5 0.75 0.448967 -0.2
425 462 5u95 (A) 400 437 39.00 0 0.1 0.323733 1.2

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_130027)

Species Accession Gene Product
Caenorhabditis elegans CELE_R07C3.4   Protein ACS-15
Caenorhabditis elegans CELE_R09E10.3   Protein ACS-18
Dictyostelium discoideum DDB_G0270106   hypothetical protein
Drosophila melanogaster Dmel_CG4501   bubblegum
Homo sapiens 23205   acyl-CoA synthetase bubblegum family member 1
Homo sapiens ENSG00000130377   acyl-CoA synthetase bubblegum family member 2
Mycobacterium leprae ML0887c   Probable long-chain-fatty-acid-CoA ligase FadD15 (FATTY-ACID-CoA SYNTHETASE) (FATTY-ACID-CoA SYNTHASE)
Mus musculus ENSMUSG00000032281   acyl-CoA synthetase bubblegum family member 1
Mus musculus ENSMUSG00000024207   acyl-CoA synthetase bubblegum family member 2
Mycobacterium tuberculosis Rv2187   Long-chain-fatty-acid-CoA ligase FadD15 (fatty-acid-CoA synthetase) (fatty-acid-CoA synthase)
Mycobacterium ulcerans MUL_3542   long-chain-fatty-acid-CoA ligase FadD15
Mycobacterium ulcerans MUL_4349   long-chain-fatty-acid-CoA ligase FadD15
Neospora caninum NCLIV_006300   very long-chain acyl-CoA synthetase, putative
Schmidtea mediterranea mk4.000084.06  
Schmidtea mediterranea mk4.000084.15  
Toxoplasma gondii TGME49_297220   AMP-binding enzyme domain-containing protein

Essentiality

ML0887c has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
TGME49_297220 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Photuris pennsylvanica Luciferase 552 aa 24.5% 474 aa Compounds References
Mycobacterium tuberculosis Fatty-acid-AMP ligase FadD28 (fatty-acid-AMP synthetase) (fatty-acid-AMP synthase) 580 aa 22.0% 509 aa Compounds References
Photinus pyralis Luciferin 4-monooxygenase 550 aa 23.3% 484 aa Compounds References
Luciola lateralis Luciferase 548 aa 25.7% 486 aa Compounds References
Photuris pennsylvanica Luciferin 4-monooxygenase 545 aa 23.1% 455 aa Compounds References
Yersinia pestis AMP-binding enzyme family protein 525 aa 23.5% 456 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Acyl Coenzyme A Synthetase (6.2.1.3 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier ML0887c (Mycobacterium leprae), Probable long-chain-fatty-acid-CoA ligase FadD15 (FATTY-ACID-CoA SYNTHETASE) (FATTY-ACID-CoA SYNTHASE)
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