Detailed view for LmjF.02.0360

Basic information

TDR Targets ID: 25629
Leishmania major, casein kinase II, alpha chain, putative

Source Database / ID:  TriTrypDB  GeneDB

pI: 8.8554 | Length (AA): 381 | MW (Da): 44198 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
24 379 1j1b (A) 35 358 25.00 0 1 1.05 -0.37
35 371 1ymi (A) 6 334 47.00 0 1 1.56 -2.02
48 367 1om1 (A) 19 330 50.00 0 1 1.6 -1.96
28 380 3uto (A) 120 427 25.00 0 1 1.02491 0.53
41 370 3war (A) 12 333 49.00 0 1 1.53354 -1.16
172 216 2cmw (A) 147 195 31.00 0.049 0.24 0.25951 0.9

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_143898)

Species Accession Gene Product
Leishmania braziliensis LbrM.02.0390   casein kinase II, alpha chain, putative
Leishmania donovani LdBPK_020330.1   casein kinase II, alpha chain, putative
Leishmania infantum LinJ.02.0330   casein kinase II, alpha chain, putative
Leishmania major LmjF.02.0360   casein kinase II, alpha chain, putative
Leishmania mexicana LmxM.02.0360   casein kinase II, alpha chain, putative
Trypanosoma brucei gambiense Tbg.972.2.980   casein kinase II, alpha chain
Trypanosoma brucei Tb927.2.2430   casein kinase II, alpha chain
Trypanosoma congolense TcIL3000_2_220   casein kinase II, alpha chain
Trypanosoma cruzi TcCLB.503513.10   casein kinase II, alpha chain, putative
Trypanosoma cruzi TcCLB.508741.320   casein kinase II, alpha chain, putative
Trichomonas vaginalis TVAG_125560   CMGC family protein kinase
Trichomonas vaginalis TVAG_044300   CMGC family protein kinase

Essentiality

LmjF.02.0360 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.2.2430 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.2.2430 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.2.2430 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.2.2430 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.7


Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 30.0% 307 aa Compounds References
Rattus norvegicus MAP kinase p38 alpha 360 aa 30.0% 307 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 30.8% 305 aa Compounds References
Zea mays Casein kinase II alpha 332 aa 49.7% 322 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 29.7% 303 aa Compounds References
Patiria pectinifera Cdc2 300 aa 29.8% 302 aa Compounds References
Rattus norvegicus Mitogen-activated protein kinase 1 358 aa 31.9% 307 aa Compounds References
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 35.1% 168 aa Compounds References
Schizosaccharomyces pombe 972h- Casein kinase II subunit alpha 332 aa 48.6% 315 aa Compounds References

Obtained from network model

Ranking Plot


Putative Drugs List


Compound Raw Global Species
0.0037 1 0.5
0.0059 1 1
0.0062 0.6935 0
0.0088 0.4477 1
0.0093 0.8828 0
0.0012 0.5 0.5
0.0016 0.5 0.5
0.0011 1 0.5
0.0069 0.3067 1
0.0042 0.5 0.5
0.0081 1 0.5
0.0039 0.9485 0.5
0.0007 0.5 0.5
0.0027 1 0.5
0.0036 0.5 0.5
0.0023 0.5 0.5
0.0056 1 0.5
0.0022 0.5 0.5
0.0039 0.5 0.5
0.0016 0.5 0.5
0.0059 1 1
0.0091 1 0.5
0.0029 0.5 0.5
0.0064 0.3377 0
0.0081 0.5 0.5
0.0061 0.6883 0.5304
0.0032 0.5 0.5
0.0033 1 1
0.0092 1 0.5
0.0032 0.5 0.5
0.0008 0.5 0.5
0.0003 0.5 0.5
0.0026 0.5 0.5
0.0007 0.5 0.5
0.0018 0.5 0.5
0.0012 0.5 0.5
0.0012 0.5 0.5
0.0063 0.7244 0.2543
0.0033 0.5 0.5
0.0004 0.5 0.5
0.0059 1 1
0.0066 0.3101 0
0.0039 0.5 0.5
0.0098 0.3242 0.2614
0.0063 1 1
0.0067 0.5 0.5
0.0007 0.5 0.5

Assayability

Assay information

  • Assay for Protein Kinase C (2.7.1.37 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

141 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.02.0360 (Leishmania major), casein kinase II, alpha chain, putative
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