Detailed view for ML2474

Basic information

TDR Targets ID: 257050
Mycobacterium leprae, possible cytidine/deoxycytidylate deaminase

Source Database / ID:  Leproma 

pI: 8.8296 | Length (AA): 171 | MW (Da): 17974 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00383   Cytidine and deoxycytidylate deaminase zinc-binding region

Gene Ontology

Mouse over links to read term descriptions.
GO:0008251   tRNA-specific adenosine deaminase activity  
GO:0002100   tRNA wobble adenosine to inosine editing  
GO:0016787   hydrolase activity  
GO:0008270   zinc ion binding  
GO:0003824   catalytic activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
10 167 2nx8 (A) 9 165 39.00 0 1 1.48788 -0.94
14 165 2b3j (A) 1 151 46.00 0 1 1.50079 -0.82
17 145 2a8n (A) 4 131 45.00 0 1 1.26479 -0.85

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126941)

Species Accession Gene Product
Arabidopsis thaliana AT1G48175   tRNA adenosine deaminase 1
Arabidopsis thaliana AT1G68720   tRNA(adenine(34)) deaminase
Babesia bovis BBOV_III000450   cytosine deaminase, putative
Brugia malayi Bm1_29930   tRNA-specific adenosine deaminase 2
Candida albicans CaO19.1458   similar to S. cerevisiae TAD2 (YJL035C) subunit of adenosine deaminase that converts the tRNA wobble position to insosine
Candida albicans CaO19.9033   similar to S. cerevisiae TAD2 (YJL035C) subunit of adenosine deaminase that converts the tRNA wobble position to insosine
Caenorhabditis elegans CELE_JC8.4   Protein JC8.4
Cryptosporidium hominis Chro.20177   cytidine and deoxycytidylate deaminase family
Cryptosporidium parvum cgd2_1630   cytidine and deoxycytidylate deaminase family, putative
Chlamydia trachomatis CT_844   tRNA-specific adenosine deaminase
Dictyostelium discoideum DDB_G0288099   adenosine deaminase, tRNA-specific
Drosophila melanogaster Dmel_CG5292   CG5292 gene product from transcript CG5292-RB
Escherichia coli b2559   tRNA-specific adenosine deaminase
Echinococcus granulosus EgrG_000788300   tRNA specific adenosine deaminase 2
Entamoeba histolytica EHI_169890   cytidine/deoxycytidylate deaminase family protein
Echinococcus multilocularis EmuJ_000788300   tRNA specific adenosine deaminase 2
Giardia lamblia GL50803_2486   Cytosine deaminase, putative
Homo sapiens ENSG00000189007   adenosine deaminase, tRNA-specific 2
Leishmania braziliensis LbrM.10.0850   deaminase, putative
Leishmania donovani LdBPK_100780.1   deaminase, putative
Leishmania infantum LinJ.10.0780   deaminase, putative
Leishmania major LmjF.10.0730   deaminase, putative
Leishmania mexicana LmxM.10.0730   deaminase, putative
Loa Loa (eye worm) LOAG_06088   tRNA-specific adenosine deaminase 2
Mycobacterium leprae ML2474   possible cytidine/deoxycytidylate deaminase
Mus musculus ENSMUSG00000019808   adenosine deaminase, tRNA-specific 2
Mycobacterium tuberculosis Rv3752c   Possible cytidine/deoxycytidylate deaminase
Mycobacterium ulcerans MUL_4370   cytidine/deoxycytidylate deaminase
Neospora caninum NCLIV_027210   Cytidine and deoxycytidylate deaminase family protein, related
Oryza sativa 4332682   Os03g0321900
Oryza sativa 4341078   Os06g0489500
Plasmodium berghei PBANKA_1362200   cytidine and deoxycytidylate deaminase, putative
Plasmodium falciparum PF3D7_1349400   cytidine and deoxycytidylate deaminase, putative
Plasmodium knowlesi PKNH_1251500   cytidine and deoxycytidylate deaminase, putative
Plasmodium vivax PVX_083365   cytidine and deoxycytidylate deaminase, putative
Plasmodium yoelii PY04017   hypothetical protein
Saccharomyces cerevisiae YPR062W   cytosine deaminase
Saccharomyces cerevisiae YJL035C   Tad2p
Schistosoma japonicum Sjp_0087990   ko:K01500 adenosine deaminase, tRNA-specific 2, TAD2 homolog [EC:3.5.4.-], putative
Schistosoma mansoni Smp_053790   cytidine/deoxycytidylate deaminase-related
Schistosoma mansoni Smp_053780   cytidine/deoxycytidylate deaminase-related
Trypanosoma brucei gambiense Tbg972.8.3890   deaminase, putative
Trypanosoma brucei Tb927.8.4180   tRNA-specific adenosine deaminase
Trypanosoma congolense TcIL3000_8_3880   deaminase, putative
Trypanosoma cruzi TcCLB.503897.80   deaminase, putative
Trypanosoma cruzi TcCLB.509561.120   deaminase, putative
Toxoplasma gondii TGME49_259570   CMP/dCMP deaminase, zinc-binding, putative
Theileria parva TP03_0793   cytidine deaminase, putative
Trichomonas vaginalis TVAG_087960   riboflavin-specific deaminase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0168   cytosine/adenosine deaminase

Essentiality

ML2474 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.4180 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.4180 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.4180 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.4180 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b2559 Escherichia coli essential goodall
CELE_JC8.4 Caenorhabditis elegans embryonic lethal wormbase
YJL035C Saccharomyces cerevisiae inviable yeastgenome
TGME49_259570 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier ML2474 (Mycobacterium leprae), possible cytidine/deoxycytidylate deaminase
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