Detailed view for TGME49_309940

Basic information

TDR Targets ID: 257331
Toxoplasma gondii, phospholipase D active site domain-containing protein
Source Database / ID:  ToxoDB 

pI: 8.0772 | Length (AA): 1186 | MW (Da): 129562 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00614   Phospholipase D Active site motif
PF13091   PLD-like domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Metabolic Pathways

Glycerophospholipid metabolism (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
15 138 1byr (A) 4 114 20.00 0.0000000016 0.84 0.38 -0.86
19 153 1byr (A) 9 146 19.00 0.00000000012 0.5 0.39 -0.9
174 239 2ys8 (A) 20 90 18.00 0.25 0.07 0.186449 -0.33
181 241 2och (A) 5 65 8.00 0.00038 0.05 0.197233 -0.8
1059 1116 1byr (A) 94 152 31.00 0.065 0.05 0.277704 0.57

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_129472)

Species Accession Gene Product
Babesia bovis BBOV_II002000   phospholipase D active site motif family protein
Cryptosporidium hominis Chro.30333   cardiolipin synthetase
Cryptosporidium parvum cgd3_2940   probable phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthase, 2x SMART_PLDc domains, possible bacterial origin
Chlamydia trachomatis CT_284   phospholipase D superfamily protein
Dictyostelium discoideum DDB_G0284687   phospholipase D
Escherichia coli b0789   cardiolipin synthase 2
Escherichia coli b1249   cardiolipin synthase 1
Leishmania braziliensis LbrM.20.1600   cardiolipin synthetase, putative
Leishmania donovani LdBPK_341860.1   cardiolipin synthetase, putative
Leishmania infantum LinJ.34.1860   cardiolipin synthetase, putative
Leishmania major LmjF.34.2110   cardiolipin synthetase, putative
Leishmania mexicana LmxM.33.2110   cardiolipin synthetase, putative
Neospora caninum NCLIV_068780   hypothetical protein
Neospora caninum NCLIV_054060   hypothetical protein
Plasmodium berghei PBANKA_0108000   mitochondrial cardiolipin synthase, putative
Plasmodium falciparum PF3D7_0609400   mitochondrial cardiolipin synthase, putative
Plasmodium knowlesi PKNH_1140800   mitochondrial cardiolipin synthase, putative
Plasmodium vivax PVX_113625   cardiolipin synthetase, putative
Plasmodium yoelii PY06901   cardiolipin synthetase
Trypanosoma brucei gambiense Tbg972.4.2520   cardiolipin synthetase, putative
Trypanosoma brucei Tb927.4.2560   cardiolipin synthetase
Trypanosoma brucei Tb11.v5.0672   cardiolipin synthetase, putative
Trypanosoma congolense TcIL3000_4_2440   cardiolipin synthetase, putative
Trypanosoma cruzi TcCLB.506631.20   cardiolipin synthetase, putative
Trypanosoma cruzi TcCLB.506559.40   cardiolipin synthetase, putative
Toxoplasma gondii TGME49_309940   phospholipase D active site domain-containing protein
Theileria parva TP04_0329   cardiolipin synthetase, putative

Essentiality

TGME49_309940 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.2560 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.2560 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.2560 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.2560 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0789 Escherichia coli non-essential goodall
b1249 Escherichia coli non-essential goodall
TGME49_309940 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.1323 0.3431 1
0.0929 0.3428 1
0.0123 0.2575 1
0.0272 0.2864 1
0.1049 0.3449 1
0.0319 0.3257 1
0.1276 0.3238 1
0.1602 0.3425 1
0.0442 0.3306 1
0.0892 0.3453 1
0.1012 0.3389 1
0.1983 0.3441 1
0.0034 0.3048 0.5
0.0097 0.3101 0.5
0.1964 0.3423 1
0.0304 0.3361 1
0.0314 0.336 1
0.0263 0.3394 1
0.0868 0.3474 1
0.0285 0.3015 0.5
0.1175 0.3472 1
0.1951 0.3411 1
0.084 0.3382 1
0.1136 0.3456 1
0.0067 0.3045 0.5
0.0035 0.2579 0.5
0.1971 0.347 1
0.0728 0.307 0.5
0.0859 0.3451 1
0.1239 0.3458 1
0.0556 0.3452 1
0.0432 0.3414 1
0.0383 0.3057 0.5
0.0868 0.3379 1
0.0311 0.324 1
0.0143 0.3192 1
0.0442 0.3306 1
0.1323 0.3431 1
0.0043 0.2613 0.5
0.1495 0.3456 1
0.087 0.3372 1
0.1708 0.3446 1
0.1347 0.3464 1
0.0648 0.3345 1
0.0127 0.2605 1
0.0613 0.3192 1
0.02 0.3304 1
0.1494 0.3265 1
0.0301 0.3317 1
0.03 0.3241 1
0.0478 0.3327 1
0.1264 0.3402 1
0.1049 0.3449 1
0.0379 0.3338 1
0.1642 0.346 1
0.1495 0.3456 1
0.1957 0.3442 1
0.1323 0.3431 1
0.1556 0.3192 1
0.0544 0.3311 1
0.157 0.3471 1
0.0304 0.2983 1
0.2048 0.3447 1
0.099 0.3452 1
0.0437 0.3294 1
0.1825 0.3433 1
0.1667 0.3465 1
0.083 0.336 1
0.0805 0.343 1
0.0865 0.3192 1
0.0316 0.3307 1
0.0733 0.3424 1
0.0336 0.326 1
0.0399 0.3359 1
0.1875 0.3459 1
0.0411 0.3382 1
0.0332 0.3014 1
0.1583 0.3436 1
0.0525 0.3328 1
0.1718 0.3463 1
0.1453 0.3435 1
0.0354 0.2706 1
0.0354 0.2706 1
0.1885 0.3442 1
0.0847 0.3401 1
0.0561 0.3059 0.5
0.1506 0.3435 1
0.1738 0.3477 1
0.175 0.3462 1
0.1444 0.3443 1
0.1971 0.347 1
0.1881 0.3192 1
0.0698 0.3286 1
0.1041 0.3435 1
0.03 0.3361 1
0.00582448 0.272382 0.5
0.1694 0.3462 1
0.0339 0.3192 1
0.1844 0.3443 1
0.0315 0.3075 1
0.1667 0.3465 1
0.0449 0.3367 1
0.1276 0.3238 1
0.124 0.3407 1
0.0268 0.3274 1
0.1134 0.347 1
0.1642 0.346 1
0.014 0.2945 1
0.2131 0.3441 1
0.0264 0.3095 0.5
0.0432 0.3414 1
0.0968 0.3389 1
0.1492 0.3399 1
0.0332 0.3014 1
0.1352 0.3451 1
0.0043 0.2504 0.5
0.016 0.2955 1
0.0579 0.3385 1
0.181 0.3465 1
0.0431 0.3311 1
0.1323 0.3431 1
0.0808 0.3466 1
0.1939 0.3424 1
0.1918 0.344 1
0.0504 0.2658 1
0.2209 0.3473 1
0.0294 0.3076 0.5
0.1049 0.3449 1
0.1959 0.3424 1
0.0814 0.3379 1
0.0254 0.3265 1
0.0201 0.3268 1
0.1623 0.344 1
0.1863 0.3478 1
0.1026 0.3351 1
0.1236 0.3431 1
0.0035 0.2582 0.5
0.1323 0.3431 1
0.1863 0.3421 1
0.1743 0.3441 1
0.0321 0.3356 1
0.1539 0.3459 1
0.0314 0.3362 1
0.1136 0.3447 1
0.1448 0.3417 1
0.1504 0.3431 1
0.0201 0.3064 0.5
0.0897 0.3192 1
0.1149 0.2888 0.5
0.0317 0.3053 1
0.2149 0.3453 1
0.1468 0.3452 1
0.1768 0.3442 1
0.1971 0.347 1
0.1164 0.3403 1
0.0352 0.3362 1
0.2174 0.345 1
0.0242 0.3192 1
0.1215 0.3373 1
0.1524 0.3426 1
0.1722 0.343 1
0.2352 0.3472 1
0.1526 0.3449 1
0.126 0.3396 1
0.0036 0.2854 0.5
0.126 0.3396 1
0.0063 0.2572 1
0.0036 0.2854 0.5
0.1528 0.3477 1
0.1667 0.3465 1
0.1939 0.3423 1
0.0197 0.33 1
0.0781 0.3319 1
0.1863 0.3478 1
0.1652 0.3192 1
0.2035 0.3434 0.4076
0.2209 0.3473 1
0.1568 0.3437 1
0.1842 0.2724 0.5
0.2209 0.3473 1
0.203 0.3464 1
0.0802 0.3369 1
0.099 0.3452 1
0.068299 0.308661 1
0.1302 0.3454 1
0.2035 0.3434 0.4076
0.1539 0.3459 1
0.0058 0.2869 1
0.0458 0.3358 1
0.0181 0.2774 1
0.0845 0.3375 1
0.0369 0.332 1
0.0808 0.3424 1
0.1483 0.3402 1
0.1012 0.3389 1
0.0892 0.3192 1
0.1339 0.3451 1
0.1336 0.3469 1
0.0344 0.3343 1
0.1997 0.3445 1
0.1467 0.3455 1
0.037 0.3258 1
0.1517 0.3472 1
0.1001 0.336 1
0.181 0.344 1
0.0398 0.3055 0.5
0.0345 0.3056 0.5
0.031 0.3082 1
0.0042 0.2573 0.5
0.1934 0.3064 0.5
0.0781 0.3319 1
0.0929 0.3428 1
0.157 0.3471 1
0.1667 0.3465 1
0.2352 0.3472 1
0.0338 0.3428 0.8148
0.0788 0.3065 0.5
0.0815 0.3192 1
0.0312 0.2976 0.5
0.1502 0.3474 1
0.0211 0.3362 1
0.0345 0.3056 0.5
0.0667 0.3132 0.5
0.1971 0.347 1
0.0583 0.3253 1
0.0311 0.324 1
0.0375 0.2715 1
0.0512 0.3343 1
0.1971 0.3443 1
0.032 0.3402 1
0.0366 0.3362 1
0.0831 0.3391 1
0.0143 0.2792 1
0.1346 0.3186 0.5
0.0264 0.3198 1
0.1854 0.3461 1
0.1374 0.3057 0.5
0.1523 0.3432 1
0.0344 0.3363 1
0.173 0.3442 1
0.0808 0.3424 1
0.0405 0.3379 1
0.0432 0.3414 1
0.071 0.3451 1
0.1716 0.3454 1
0.2209 0.3473 1
0.0892 0.3453 1
0.0308 0.327 1
0.039 0.3398 1
0.0042 0.2951 0.5
0.1921 0.341 1
0.0972 0.3381 1
0.1379 0.346 1
0.203 0.3464 1
0.0406 0.3349 1
0.0317 0.3053 1
0.0321 0.3438 1
0.0035 0.2598 0.5
0.224 0.3436 1
0.2146 0.3451 1
0.0314 0.3362 1
0.1359 0.3475 1
0.0042 0.2833 0.5
0.2317 0.2554 0.5
0.2352 0.3472 1
0.0711 0.3359 1
0.2106 0.3445 1
0.039 0.3398 1
0.0131 0.3067 1
0.0251 0.2713 0.5
0.0327 0.3322 1
0.0501 0.3327 1
0.0159 0.2992 1
0.0907 0.336 1
0.0432 0.3414 1
0.0393 0.2504 0.5
0.0245 0.3136 1
0.1158 0.3469 1
0.2007 0.3035 0.5
0.166 0.344 1
0.0398 0.3402 1
0.0504 0.2658 1
0.1622 0.3469 1
0.0259 0.2975 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_309940 (Toxoplasma gondii), phospholipase D active site domain-containing protein
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