Detailed view for TGME49_267270

Basic information

TDR Targets ID: 257423
Toxoplasma gondii, divalent metal transporter, putative
Source Database / ID:  ToxoDB 

pI: 6.5042 | Length (AA): 1227 | MW (Da): 132880 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 11

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01566   Natural resistance-associated macrophage protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0016020   membrane  
GO:0005215   transporter activity  
GO:0046873   metal ion transmembrane transporter activity  
GO:0006810   transport  
GO:0030001   metal ion transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
219 290 5bs7 (E) 615 669 27.00 0 0.04 -0.0182203 -0.19
657 1131 5m87 (A) 24 504 29.00 0 1 0.644223 0.03
666 1083 5m87 (A) 32 468 34.00 0 1 0.660668 0.02
672 1076 5kte (A) 43 427 36.00 0 0.75 0.382073 0.95
672 1077 5kte (A) 43 426 32.00 0 1 0.466988 0.54
681 1083 5m94 (A) 45 445 30.00 0 0.98 0.653543 -0.14

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_127097)

Species Accession Gene Product
Arabidopsis thaliana AT1G15960   metal transporter Nramp6
Arabidopsis thaliana AT1G47240   metal transporter NRAMP2
Arabidopsis thaliana AT1G80830   metal transporter Nramp1
Arabidopsis thaliana AT4G18790   metal transporter Nramp5
Arabidopsis thaliana AT5G67330   metal transporter Nramp4
Arabidopsis thaliana AT2G23150   metal transporter Nramp3
Brugia malayi Bm1_40010   NRAMP-like transporter K11G12.4
Candida albicans CaO19.2069   probable NRAMP divalent cation transporter similar to S. cerevisiae SMF3 (YLR034C) vacuolar metal transporter
Candida albicans CaO19.12159   one of two probable NRAMP divalent cation transporter genes similar to S. cerevisiae SMF1 (YOL122C) cell surface manganese trans
Candida albicans CaO19.9616   probable NRAMP divalent cation transporter similar to S. cerevisiae SMF3 (YLR034C) vacuolar metal transporter
Candida albicans CaO19.2270   one of two probable NRAMP divalent cation transporter genes similar to S. cerevisiae SMF1 (YOL122C) cell surface manganese trans
Candida albicans CaO19.9810   one of two probable NRAMP divalent cation transporter genes similar to S. cerevisiae SMF1 (YOL122C) cell surface manganese trans
Candida albicans CaO19.4690   one of two probable NRAMP divalent cation transporter genes similar to S. cerevisiae SMF1 (YOL122C) cell surface manganese trans
Caenorhabditis elegans CELE_K11G12.3   Protein SMF-2
Caenorhabditis elegans CELE_K11G12.4   Protein SMF-1, isoform B
Caenorhabditis elegans CELE_Y69A2AR.4   Protein SMF-3
Dictyostelium discoideum DDB_G0276973   natural resistance-associated macrophage protein
Dictyostelium discoideum DDB_G0275815   natural resistance-associated macrophage protein
Drosophila melanogaster Dmel_CG3671   Malvolio
Escherichia coli b2392   manganese/divalent cation transporter
Echinococcus granulosus EgrG_000945600   divalent metal transporter DMT1B
Echinococcus multilocularis EmuJ_000945600   divalent metal transporter DMT1B
Homo sapiens ENSG00000110911   solute carrier family 11 (proton-coupled divalent metal ion transporter), member 2
Homo sapiens ENSG00000018280   solute carrier family 11 (proton-coupled divalent metal ion transporter), member 1
Loa Loa (eye worm) LOAG_13171   hypothetical protein
Loa Loa (eye worm) LOAG_05166   hypothetical protein
Mycobacterium leprae ML2098   DIVALENT CATION-TRANSPORT INTEGRAL MEMBRANE PROTEIN MNTH (BRAMP) (MRAMP)
Mus musculus ENSMUSG00000026177   solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1
Mus musculus ENSMUSG00000023030   solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2
Mycobacterium tuberculosis Rv0924c   Divalent cation-transport integral membrane protein MntH (BRAMP) (MRAMP)
Mycobacterium ulcerans MUL_4445   manganese transport protein MntH
Neospora caninum NCLIV_038890   hypothetical protein
Oryza sativa 9272162   Os01g0733001
Oryza sativa 4342859   Os07g0257200
Oryza sativa 4342862   Os07g0258400
Oryza sativa 4328200   Os02g0131800
Oryza sativa 4352640   Os12g0581600
Oryza sativa 4332012   Os03g0208500
Onchocerca volvulus OVOC5136   Protein Malvolio homolog
Plasmodium berghei PBANKA_1238600   transporter, putative
Plasmodium falciparum PF3D7_0523800   transporter, putative
Plasmodium knowlesi PKNH_1009100   transporter, putative
Plasmodium vivax PVX_080060   metal transporter, putative
Plasmodium yoelii PY04720   putative metal ion transporter
Saccharomyces cerevisiae YOL122C   divalent metal ion transporter SMF1
Saccharomyces cerevisiae YLR034C   putative divalent metal ion transporter SMF3
Schistosoma japonicum Sjp_0301090   ko:K03322 metal ion transporter, Nramp family, putative
Schistosoma mansoni Smp_099850.3   divalent metal transporter DMT1B
Schistosoma mansoni Smp_099850.4   divalent metal transporter DMT1B
Schmidtea mediterranea mk4.022723.00   Protein Malvolio
Schmidtea mediterranea mk4.037519.00  
Schmidtea mediterranea mk4.009244.00   Protein Malvolio
Schmidtea mediterranea mk4.004761.00  
Toxoplasma gondii TGME49_267270   divalent metal transporter, putative

Essentiality

TGME49_267270 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
b2392 Escherichia coli non-essential goodall
TGME49_267270 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens solute carrier family 11 (proton-coupled divalent metal ion transporter), member 1 Compounds References
Homo sapiens solute carrier family 11 (proton-coupled divalent metal ion transporter), member 2 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0105 1 0.5
0.0122 0.5857 1
0.0442 1 1
0.0597 1 0.5
0.0117 0.3987 1
0.0171 0.5802 1
0.012 0.4868 0.5
0.0442 1 1
0.0582 1 0.5
0.0521 1 0.5
0.0351 1 0.5
0.0163 1 1
0.0159 1 0.5
0.0222 0.7341 1
0.0276 1 1
0.0404 0.3075 0.5
0.0175 0.519 1
0.0065 0.3008 0.3652
0.0293 0.285 1
0.0117 0.3987 1
0.0651 0.8196 1
0.0495 0.7309 1
0.0398 1 1
0.0519 1 0.5
0.0504 0.3962 0.5
0.0587 1 0.5
0.0419 1 0.5
0.0169 0.4591 0.5
0.0352 1 0.5
0.0281 0.843 1
0.058 1 0.5
0.0107 0.5 0.5
0.0289 0.7034 0.5
0.0293 0.338 0.5
0.0161 0.3197 1
0.058 0.5809 0.5
0.0354 0.3569 1
0.0825 1 1
0.0225 0.6682 1
0.0303 1 1
0.0432 1 0.5
0.0225 0.6066 1
0.0495 0.7309 1
0.0226 0.8404 0.5
0.0305 0.2943 1
0.0231 0.5904 0.5
0.0065 0.2819 0
0.0169 0.2635 0.5
0.0582 1 0.5
0.0651 0.8196 1
0.0516 1 0.5
0.0563 1 0.5
0.0286 0.6801 0.5
0.0117 0.4619 1
0.0164 1 1
0.0522 1 0.5
0.0519 1 0.5
0.0543 1 0.5
0.0065 1 1
0.0124 0.2968 1
0.0519 1 0.5
0.0124 0.3484 1
0.0412 1 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_267270 (Toxoplasma gondii), divalent metal transporter, putative
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