Detailed view for TGME49_281450

Basic information

TDR Targets ID: 257956
Toxoplasma gondii, cell-cycle-associated protein kinase, putative
Source Database / ID:  ToxoDB 

pI: 9.5633 | Length (AA): 454 | MW (Da): 50169 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0004672   protein kinase activity  
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
6 439 3pvu (A) 117 551 18.00 0 1 0.901347 0.48
11 53 2mu3 (A) 135 177 49.00 0.042 0.08 0.482914 0.8
76 387 5hq0 (A) 0 289 45.00 0 1 1.20142 -0.84
77 387 3o0g (A) 1 288 44.00 0 1 1.14892 -0.85

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Tachyzoite, ME49 merozoite. Gregory Hehl AB
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_128263)

Species Accession Gene Product
Arabidopsis thaliana AT5G10270   1
Arabidopsis thaliana AT5G64960   cyclin-dependent kinase C-2
Brugia malayi Bm1_46210   Protein kinase domain containing protein
Candida albicans CaO19.1619   Rpo21 C-terminus domain kinase
Candida albicans CaO19.9187   Rpo21 C-terminus domain kinase
Caenorhabditis elegans CELE_B0285.1   Protein CDK-12, isoform B
Dictyostelium discoideum DDB_G0273207   CDK family protein kinase
Dictyostelium discoideum DDB_G0273721   CDK family protein kinase
Drosophila melanogaster Dmel_CG7597   CG7597 gene product from transcript CG7597-RB
Echinococcus granulosus EgrG_001041300   Cell division cycle 2 protein kinase
Echinococcus multilocularis EmuJ_001041300   Cell division cycle 2 protein kinase
Homo sapiens ENSG00000065883   cyclin-dependent kinase 13
Homo sapiens ENSG00000167258   cyclin-dependent kinase 12
Loa Loa (eye worm) LOAG_03355   hypothetical protein
Loa Loa (eye worm) LOAG_04967   CMGC/CDK/CRK7 protein kinase
Mus musculus ENSMUSG00000041297   cyclin-dependent kinase 13
Mus musculus ENSMUSG00000003119   cyclin-dependent kinase 12
Neospora caninum NCLIV_023400   hypothetical protein
Oryza sativa 9268645   Os08g0453800
Oryza sativa 4338674   Os05g0389700
Oryza sativa 4325386   Os01g0958000
Saccharomyces cerevisiae YKL139W   Ctk1p
Schistosoma japonicum Sjp_0094410   Cell division cycle 2-like protein kinase 5, putative
Schistosoma japonicum Sjp_0094420   ko:K08819 Cdc2-related kinase, arginine/serine-rich, putative
Schistosoma mansoni Smp_007510   protein kinase
Schmidtea mediterranea mk4.000235.06   Cyclin-dependent kinase 12
Schmidtea mediterranea mk4.004836.01  
Schmidtea mediterranea mk4.012172.00   Cyclin-dependent kinase 12
Toxoplasma gondii TGME49_281450   cell-cycle-associated protein kinase, putative
Trichomonas vaginalis TVAG_359930   CMGC family protein kinase
Trichomonas vaginalis TVAG_249190   CMGC family protein kinase
Trichomonas vaginalis TVAG_265810   CMGC family protein kinase
Trichomonas vaginalis TVAG_235250   CMGC family protein kinase

Essentiality

TGME49_281450 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_B0285.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_B0285.1 Caenorhabditis elegans larval arrest wormbase
CELE_B0285.1 Caenorhabditis elegans larval lethal wormbase
CELE_B0285.1 Caenorhabditis elegans slow growth wormbase
CELE_B0285.1 Caenorhabditis elegans sterile wormbase
YKL139W Saccharomyces cerevisiae inviable yeastgenome
TGME49_281450 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens cyclin-dependent kinase 12 Compounds References
Homo sapiens cyclin-dependent kinase 13 Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Cell division protein kinase 5 292 aa 41.7% 309 aa Compounds References
Xenopus laevis Aurora kinase B-A 361 aa 25.7% 377 aa Compounds References
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 41.4% 203 aa Compounds References
Rattus norvegicus MAP kinase p38 alpha 360 aa 32.1% 302 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 41.5% 313 aa Compounds References
Rattus norvegicus Mitogen-activated protein kinase 1 358 aa 34.4% 305 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 38.5% 304 aa Compounds References
Xenopus laevis Aurora kinase B-B 368 aa 24.4% 352 aa Compounds References
Patiria pectinifera Cdc2 300 aa 38.6% 319 aa Compounds References
Schizosaccharomyces pombe 972h- Casein kinase II subunit alpha 332 aa 25.1% 319 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0066 0.3101 0
0.0008 0.5 0.5
0.0011 1 0.5
0.0033 0.5 0.5
0.0063 1 1
0.0023 0.5 0.5
0.0007 0.5 0.5
0.0039 0.5 0.5
0.0092 1 0.5
0.0063 0.7244 0.7244
0.0007 0.5 0.5
0.0004 0.5 0.5
0.0059 1 1
0.0003 0.5 0.5
0.0036 0.5 0.5
0.0039 0.9485 0.5
0.0007 0.5 0.5
0.0062 0.6935 0
0.0032 0.5 0.5
0.0033 1 1
0.0032 0.5 0.5
0.0061 0.6883 0.6656
0.0042 0.5 0.5
0.0064 0.3377 0
0.0018 0.5 0.5
0.0081 1 0.5
0.0059 1 1
0.0022 0.5 0.5
0.0093 0.8828 0
0.0012 0.5 0.5
0.0016 0.5 0.5
0.0081 0.5 0.5
0.0027 1 0.5
0.0091 1 0.5
0.0067 0.5 0.5
0.0012 0.5 0.5
0.0059 1 1
0.0088 0.4477 1
0.0069 0.3067 1
0.0098 0.3242 0.2614
0.0016 0.5 0.5
0.0012 0.5 0.5
0.0029 0.5 0.5
0.0039 0.5 0.5
0.0037 1 0.5
0.0056 1 0.5
0.0026 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

12 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_281450 (Toxoplasma gondii), cell-cycle-associated protein kinase, putative
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