Detailed view for TGME49_270330

Basic information

TDR Targets ID: 258132
Toxoplasma gondii, cell-cycle-associated protein kinase, putative
Source Database / ID:  ToxoDB 

pI: 8.3392 | Length (AA): 425 | MW (Da): 46506 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0070985   GO:TFIIK complex  

GO:0008353   RNA polymerase subunit kinase activity  
GO:0005675   holo TFIIH complex  
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
27 424 2xrw (A) 2 340 29.00 0 1 0.930571 0.69
44 417 4gcj (A) 0 298 41.00 0 1 1.0901 0.23
46 419 2baj (A) 19 319 43.00 0 1 1.0029 0.24
47 407 4au8 (A) 0 288 47.00 0 1 1.05251 0.2
278 407 2i6l (B) 196 318 37.00 0 1 0.679582 -0.43

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 merozoite. Gregory Hehl AB
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_141009)

Species Accession Gene Product
Babesia bovis BBOV_IV010230   cyclin-dependent kinase-related kinase, putative
Cryptosporidium hominis Chro.70056   cyclin-dependent kinase-related kinase
Cryptosporidium parvum cgd7_430   cyclin-dependent kinase-related kinase, putative
Neospora caninum NCLIV_036330   CAM kinase, CDPK family, putative
Plasmodium berghei PBANKA_1212800   MO15-related protein kinase
Plasmodium falciparum PF3D7_1014400   MO15-related protein kinase
Plasmodium knowlesi PKNH_0814500   MO15-related protein kinase
Plasmodium vivax PVX_094935   cyclin dependent kinase 7 (cdk7), putative
Plasmodium yoelii PY05139   MO15-related protein kinase Pfmrk
Toxoplasma gondii TGME49_270330   cell-cycle-associated protein kinase, putative
Theileria parva TP01_0781   cyclin-dependent kinase, putative

Essentiality

TGME49_270330 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1212800 Plasmodium berghei Essential plasmo
TGME49_270330 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.7

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Plasmodium falciparum MO15-related protein kinase Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 33.7% 208 aa Compounds References
Zea mays Casein kinase II alpha 332 aa 27.8% 284 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 35.1% 353 aa Compounds References
Patiria pectinifera Cdc2 300 aa 34.3% 356 aa Compounds References
Xenopus laevis Aurora kinase B-A 361 aa 21.8% 358 aa Compounds References
Rattus norvegicus Mitogen-activated protein kinase 1 358 aa 28.6% 371 aa Compounds References
Schizosaccharomyces pombe 972h- Casein kinase II subunit alpha 332 aa 24.0% 296 aa Compounds References
Rattus norvegicus Cell division protein kinase 5 292 aa 36.1% 360 aa Compounds References
Rattus norvegicus MAP kinase p38 alpha 360 aa 33.6% 381 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 35.1% 353 aa Compounds References
Bos taurus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 21.5% 289 aa Compounds References
Rattus norvegicus Serine/threonine-protein kinase pim-3 326 aa 21.1% 279 aa Compounds References
Xenopus laevis Aurora kinase B-B 368 aa 21.5% 358 aa Compounds References
Rattus norvegicus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 21.5% 289 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0238 0.9237 1
0.0346 0.3058 1
0.0121 0.2626 1
0.0135 0.9293 1
0.011 0.3097 1
0.0119 0.6176 1
0.0123 0.3423 1
0.014919 0.488888 0.709495
0.0224 0.9259 1
0.0081 1 0.5
0.0109 0.3097 1
0.0126 0.3097 1
0.0135 0.7173 0.847
0.0115 0.2665 0.6765
0.0116 0.6972 1
0.0116 0.6583 1
0.012 0.3097 1
0.0029 0.5 0.5
0.0135 0.2605 0
0.0113 0.6972 1
0.0092 1 0.5
0.0131 0.4089 1
0.0022 0.5 0.5
0.023 0.2833 1
0.0178198 0.269965 0.895144
0.0056 1 0.5
0.0162128 0.925042 1
0.0615882 0.924862 1
0.0039 0.9485 0.5
0.0003 0.5 0.5
0.0135 0.2787 0.7313
0.0112 0.4638 1
0.0116 0.9324 1
0.0012 0.5 0.5
0.0466 0.4372 0.8859
0.0231 0.6042 1
0.0115 0.5637 1
0.0601 0.6983 1
0.011 0.3097 1
0.0012 0.5 0.5
0.0317709 0.342281 0.739187
0.0152212 0.924623 0.5
0.0111 0.2623 1
0.0145855 0.391145 1
0.0108 0.52 1
0.012 0.4572 1
0.0217 0.2587 1
0.0059 1 1
0.0114 0.5525 1
0.0108 0.3083 1
0.0007 0.5 0.5
0.0109 0.6488 1
0.0128 0.3093 1
0.0122 0.4298 1
0.0113 0.4157 1
0.0108 0.2904 1
0.0111 0.6972 1
0.0116 0.655 1
0.0112 0.3457 1
0.0235 0.921 1
0.0033 0.5 0.5
0.011 0.3286 1
0.0111 0.3924 1
0.0121 0.78 1
0.0114 0.6556 0.6022
0.0135 0.3316 0.3316
0.0115 0.6972 1
0.0115 0.7458 1
0.0111 0.3097 1
0.0111 0.5853 0.5
0.023 0.2833 1
0.0115 0.9246 1
0.011 0.6492 1
0.011 0.6176 1
0.0108 0.3083 1
0.0011 1 0.5
0.0117 0.9301 1
0.0603 0.9329 1
0.0135 0.2904 1
0.0111 0.3112 1
0.0244 0.2588 0.3223
0.0007 0.5 0.5
0.0108 0.6311 1
0.0108 0.3083 1
0.0033 1 1
0.0458 0.4308 0.863
0.0135 0.521 1
0.0242 0.9157 1
0.011 0.2664 0.676
0.0037 1 0.5
0.0091 1 0.5
0.0347 0.9293 1
0.0706 0.8743 1
0.0603 0.6961 1
0.0067 0.5 0.5
0.0123 0.2668 0.6772
0.0458 0.2616 0.6508
0.0111 0.9318 1
0.0116 0.3097 1
0.0135 0.3233 0.5685
0.0229 0.928 1
0.0231 0.9171 1
0.0114 0.6095 1
0.0062 0.6935 0
0.0098 0.3242 0.2614
0.015778 0.924623 0.5
0.0337 0.9327 1
0.0231 0.2678 1
0.0111 0.3499 1
0.0135 0.4665 1
0.0111 0.6924 1
0.0122 0.9324 1
0.0111 0.6193 1
0.002 0.2516 1
0.0241 0.7308 1
0.0118 0.3097 1
0.0226 0.9205 1
0.0345 0.4142 1
0.0239 0.6433 1
0.0111 0.2839 1
0.011 0.3097 1
0.0063 0.7244 0.7244
0.0113 0.3483 1
0.0122 0.6572 0.5
0.0339 0.4214 1
0.0246 0.2506 0.3733
0.0088 0.4477 1
0.0147014 0.928045 1
0.034 0.2715 1
0.0115 0.9125 1
0.0228 0.921 1
0.0135 0.2605 0
0.0111 0.3759 0.5
0.0135 0.7588 1
0.002 0.3264 1
0.0016 0.5 0.5
0.011 0.2891 1
0.0027 1 0.5
0.0599 0.933 1
0.0135 0.2904 1
0.0039 0.5 0.5
0.0111 0.3097 1
0.0137 0.3485 1
0.0716 0.8576 1
0.0116 0.9336 1
0.0113 0.3097 1
0.024 0.5933 1
0.0118 0.2848 1
0.0238 0.9293 0.5
0.0147014 0.928045 1
0.0234 0.9298 1
0.0123 0.3013 1
0.0458 0.4308 0.863
0.0004 0.5 0.5
0.0113 0.3097 1
0.0115 0.3097 1
0.0113 0.3272 1
0.0249 0.5432 1
0.0354 0.9006 1
0.0109 0.6176 1
0.0107 0.362 0.5
0.0118 0.3097 1
0.0018 0.5 0.5
0.0223 0.9167 1
0.0007 0.5 0.5
0.0069 0.3067 1
0.0066 0.3101 0
0.0128 0.3093 1
0.0118 0.9293 1
0.0063 1 1
0.0008 0.5 0.5
0.0336 0.2969 0.8682
0.0093 0.8828 0
0.0111 0.9295 0.5
0.0081 0.5 0.5
0.0217 0.2587 1
0.0042 0.5 0.5
0.0135 0.3233 0.5685
0.0108 0.9295 0.5
0.0135 0.2587 1
0.0135 0.9293 0.5
0.0122 0.7456 1
0.0128 0.3093 1
0.0036 0.5 0.5
0.0032 0.5 0.5
0.0359 0.3533 1
0.023 0.5434 1
0.0113 0.5327 1
0.0023 0.5 0.5
0.0126 0.711 0.5
0.0224 0.9253 1
0.0012 0.5 0.5
0.0116 0.6583 1
0.0135 0.3112 1
0.0111 0.3097 1
0.0112 0.6694 0.5
0.0334 0.647 1
0.0337 0.9303 1
0.0118 0.3045 0.8498
0.0026 0.5 0.5
0.023 0.2833 1
0.0064 0.3377 0
0.0061 0.6883 0.6656
0.0112 0.6972 1
0.0116 0.3097 1
0.0253 0.672 1
0.0726 0.8639 1
0.0241 0.927 1
0.011 0.655 0.5
0.0109 0.4218 1
0.023 0.9167 1
0.0118 0.6351 0.5
0.0039 0.5 0.5
0.0059 1 1
0.013 0.3112 1
0.0111 0.298 1
0.0059 1 1
0.0115 0.3105 1
0.0016 0.5 0.5
0.0111 0.3892 1
0.0224 0.5303 1
0.0116 0.277 1
0.0118 0.3842 1
0.0135 0.9307 1
0.011 0.3394 1
0.0119 0.9306 0.5
0.0114 0.3114 1
0.0111 0.3097 1
0.0111 0.6175 1
0.0135 0.3112 1
0.0032 0.5 0.5
0.024 0.5597 1
0.0231 0.921 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

12 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_270330 (Toxoplasma gondii), cell-cycle-associated protein kinase, putative
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