Detailed view for TGME49_214230

Basic information

TDR Targets ID: 258401
Toxoplasma gondii, Dopey, N-terminal domain-containing protein
Source Database / ID:  ToxoDB 

pI: 6.4211 | Length (AA): 3107 | MW (Da): 333940 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04118   Dopey, N-terminal

Gene Ontology

Mouse over links to read term descriptions.
GO:0005488   binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
850 912 5hmo (A) 803 861 44.00 0.4 0.02 0.563377 -1.75
867 913 2maj (A) 0 348 28.00 0 0.03 0.471527 -3.04
1017 1820 3ea5 (D) 6 814 13.00 0 1 0.302171 0.81
2006 2080 4cwu (Q) 12 90 31.00 0.97 0.02 -0.051761 2.74
2757 3005 5cwi (A) 4 234 12.00 0 0.04 0.147542 -0.59

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_128921)

Species Accession Gene Product
Babesia bovis BBOV_I003740   dopey, N-terminal domain containing protein
Brugia malayi Bm1_04370   Dopey, N-terminal domain containing protein
Brugia malayi Bm1_54675   hypothetical protein
Candida albicans CaO19.10675   similar to Aspergillus nidulans dopA
Candida albicans CaO19.3166   similar to Aspergillus nidulans dopA
Caenorhabditis elegans CELE_Y18D10A.13   Protein PAD-1
Drosophila melanogaster Dmel_CG15099   CG15099 gene product from transcript CG15099-RB
Echinococcus granulosus EgrG_000045200   protein dopey 1
Echinococcus multilocularis EmuJ_000045200   protein dopey 1
Homo sapiens ENSG00000083097   dopey family member 1
Homo sapiens ENSG00000142197   dopey family member 2
Loa Loa (eye worm) LOAG_04088   hypothetical protein
Mus musculus ENSMUSG00000034973   dopey family member 1
Mus musculus ENSMUSG00000022946   dopey family member 2
Neospora caninum NCLIV_051470   dopey, N-terminal domain-containing protein, putative
Saccharomyces cerevisiae YDR141C   Dop1p
Schistosoma japonicum Sjp_0203630   expressed protein
Schistosoma mansoni Smp_164120   dopey-related
Schistosoma mansoni Smp_164130   dopey-related
Schmidtea mediterranea mk4.015431.00  
Schmidtea mediterranea mk4.015679.04   Dopey-related
Schmidtea mediterranea mk4.008868.03   Dopey-related
Schmidtea mediterranea mk4.014427.00  
Schmidtea mediterranea mk4.008868.02   Dopey-related
Schmidtea mediterranea mk4.009193.04   Dopey-related
Schmidtea mediterranea mk4.009193.05   Dopey-related
Toxoplasma gondii TGME49_214230   Dopey, N-terminal domain-containing protein
Theileria parva TP01_0466   hypothetical protein, conserved

Essentiality

TGME49_214230 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_Y18D10A.13 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y18D10A.13 Caenorhabditis elegans slow growth wormbase
CELE_Y18D10A.13 Caenorhabditis elegans sterile wormbase
YDR141C Saccharomyces cerevisiae inviable yeastgenome
TGME49_214230 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

No user comments are available for this gene. Log in to add comments, or register.

Enter your comment

User ()
Gene identifier TGME49_214230 (Toxoplasma gondii), Dopey, N-terminal domain-containing protein
Title for this comment
Comment