Detailed view for TGME49_238180

Basic information

TDR Targets ID: 258443
Toxoplasma gondii, 26s proteasome regulatory complex subunit, putative
Source Database / ID:  ToxoDB 

pI: 7.5823 | Length (AA): 417 | MW (Da): 47551 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01399   PCI domain
PF10602   26S proteasome subunit RPN7

Gene Ontology

Mouse over links to read term descriptions.
GO:0030234   enzyme regulator activity  
GO:0005515   protein binding  

Metabolic Pathways

Proteasome (KEGG)

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
27 411 4d10 (A) 77 489 19.00 0.000000000006 1 1.13346 0.03
47 366 4lct (B) 48 377 21.00 0 1 1.07199 -0.57
47 417 4d10 (A) 96 495 23.00 0 1 1.15629 -0.23
136 237 3b5k (A) 37 109 45.00 0.61 0.12 0.115804 1.95

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 merozoite. Gregory Hehl AB
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_127957)

Species Accession Gene Product
Arabidopsis thaliana AT4G24820   26S proteasome regulatory subunit Rpn7
Babesia bovis BBOV_II001850   PCI domain containing 26S proteasome non-ATPase regulatory subunit 6 protein, putative
Brugia malayi Bm1_41265   Probable 26S proteasome non-ATPase regulatory subunit 6
Candida albicans CaO19.7286   similar to S. cerevisiae RPN7 (YPR108W) subunit of the 19S regulatory particle of the 26S proteasome
Caenorhabditis elegans CELE_F49C12.8   Protein RPN-7
Cryptosporidium hominis Chro.20238   hypothetical protein
Cryptosporidium parvum cgd2_2210   proteasome regulatory subunit Rpn7/26S proteasome subunit 6, PINT domain containing protein
Dictyostelium discoideum DDB_G0270188   26S proteasome non-ATPase regulatory subunit 6
Drosophila melanogaster Dmel_CG5378   Regulatory particle non-ATPase 7
Echinococcus granulosus EgrG_000960400   26S proteasome non ATPase regulatory subunit 6
Entamoeba histolytica EHI_054230   proteasome regulatory subunit, putative
Echinococcus multilocularis EmuJ_000960400   26S proteasome non ATPase regulatory subunit 6
Giardia lamblia GL50803_4331   26S proteasome non-ATPase regulatory subunit 6
Homo sapiens ENSG00000163636   proteasome (prosome, macropain) 26S subunit, non-ATPase, 6
Leishmania braziliensis LbrM.32.3060   proteasome regulatory non-ATP-ase subunit, putative
Leishmania donovani LdBPK_322960.1   proteasome regulatory non-ATP-ase subunit, putative
Leishmania infantum LinJ.32.2960   proteasome regulatory non-ATP-ase subunit, putative
Leishmania major LmjF.32.2820   proteasome regulatory non-ATP-ase subunit, putative
Leishmania mexicana LmxM.31.2820   proteasome regulatory non-ATP-ase subunit, putative
Loa Loa (eye worm) LOAG_02683   proteasome Regulatory Particle
Mus musculus ENSMUSG00000021737   proteasome (prosome, macropain) 26S subunit, non-ATPase, 6
Neospora caninum NCLIV_015550   26S proteasome non-ATPase regulatory subunit, putative
Oryza sativa 4336206   Os04g0485000
Oryza sativa 4329887   Os02g0600100
Plasmodium berghei PBANKA_0919000   26S proteasome regulatory subunit RPN7, putative
Plasmodium falciparum PF3D7_1129200   26S proteasome regulatory subunit RPN7, putative
Plasmodium knowlesi PKNH_0927200   26S proteasome regulatory subunit RPN7, putative
Plasmodium vivax PVX_092075   26S proteasome regulatory subunit RPN7, putative
Plasmodium yoelii PY06923   Homo sapiens KIAA0107-like protein
Saccharomyces cerevisiae YPR108W   proteasome regulatory particle lid subunit RPN7
Schistosoma japonicum Sjp_0207640   ko:K03037 26S proteasome regulatory subunit N7, putative
Schistosoma mansoni Smp_052870   26S proteasome non-ATPase regulatory subunit
Schmidtea mediterranea mk4.013794.00   26S proteasome non-ATPase regulatory subunit 6
Schmidtea mediterranea mk4.010047.00   26S proteasome non-ATPase regulatory subunit 6
Trypanosoma brucei gambiense Tbg972.11.17960   proteasome regulatory non-ATP-ase subunit 7, putative
Trypanosoma brucei Tb927.11.16030   proteasome regulatory non-ATP-ase subunit 7
Trypanosoma congolense TcIL3000.11.15940   proteasome regulatory non-ATP-ase subunit 7
Trypanosoma cruzi TcCLB.509073.119   proteasome regulatory non-ATP-ase subunit 7, putative
Trypanosoma cruzi TcCLB.508707.90   proteasome regulatory non-ATPase subunit 7, putative
Toxoplasma gondii TGME49_238180   26s proteasome regulatory complex subunit, putative
Theileria parva TP04_0395   26S proteasome regulatory subunit, putative
Trichomonas vaginalis TVAG_371680   cop9 signalosome complex subunit, putative

Essentiality

TGME49_238180 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.7690 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.01.7690 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.7690 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.7690 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F49C12.8 Caenorhabditis elegans embryonic lethal wormbase
CELE_F49C12.8 Caenorhabditis elegans larval lethal wormbase
CELE_F49C12.8 Caenorhabditis elegans slow growth wormbase
CELE_F49C12.8 Caenorhabditis elegans sterile wormbase
YPR108W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0919000 Plasmodium berghei Essential plasmo
TGME49_238180 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens proteasome (prosome, macropain) 26S subunit, non-ATPase, 6 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TGME49_238180 (Toxoplasma gondii), 26s proteasome regulatory complex subunit, putative
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