Detailed view for TGME49_224190

Basic information

TDR Targets ID: 258622
Toxoplasma gondii, cation-transporting atpase family protein
Source Database / ID:  ToxoDB 

pI: 8.139 | Length (AA): 1256 | MW (Da): 139821 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 10

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00702   haloacid dehalogenase-like hydrolase
PF16209   Phospholipid-translocating ATPase N-terminal
PF16212   Phospholipid-translocating P-type ATPase C-terminal

Gene Ontology

Mouse over links to read term descriptions.
GO:0016021   integral to membrane  
GO:0016020   membrane  
GO:0005524   ATP binding  
GO:0004012   phospholipid-translocating ATPase activity  
GO:0003824   catalytic activity  
GO:0000287   magnesium ion binding  
GO:0000166   nucleotide binding  
GO:0015914   phospholipid transport  
GO:0008152   metabolic process  
GO:0006754   ATP biosynthetic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
338 937 1wpg (A) 206 765 26.00 0 0.97 0.54 0.69
443 1055 4nab (A) 80 765 26.00 0.000000000024 0.95 0.407757 1.6
675 858 1mo7 (A) 420 593 18.00 0 0.64 -0.0114032 1.09
848 941 5a5w (A) 46 132 33.00 0.98 0.15 0.337541 -0.03
990 1024 3r4c (A) 205 238 44.00 0.64 0.32 0.444566 0.3

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_127596)

Species Accession Gene Product
Babesia bovis BBOV_IV003630   phospholipid-translocating P-type ATPase, putative
Brugia malayi Bm1_27560   potential phospholipid-transporting ATPase IIB, putative
Candida albicans CaO19.8405   ATPase
Candida albicans CaO19.783   ATPase
Caenorhabditis elegans CELE_F36H2.1   Protein TAT-5, isoform C
Caenorhabditis elegans CELE_F02C9.3   Protein TAT-6
Cryptosporidium hominis Chro.20257   ATPas, class II, type 9B; ATPase, class 2, member b; ATPase 9B, p type; ATPase 9B, class II
Cryptosporidium parvum cgd2_2400   ATPase, class II, type 9B, putative
Dictyostelium discoideum DDB_G0270870   ATPase, class II, type 9B
Drosophila melanogaster Dmel_CG31729   CG31729 gene product from transcript CG31729-RB
Echinococcus granulosus EgrG_000172600   phospholipid transporting ATPase IIB
Entamoeba histolytica EHI_197300   phospholipid-transporting ATPase Neo1, putative
Entamoeba histolytica EHI_049640   phospholipid-transporting P-type ATPase, putative
Entamoeba histolytica EHI_168260   phospholipid-transporting P-type ATPase, putative
Echinococcus multilocularis EmuJ_000172600   phospholipid transporting ATPase IIB
Giardia lamblia GL50803_16958   Phospholipid-transporting ATPase IA, putative
Giardia lamblia GL50803_137725   Phospholipid-transporting ATPase IIB, putative
Giardia lamblia GL50803_101810   Phospholipid-transporting ATPase IIB, putative
Giardia lamblia GL50803_88438   ATPase
Giardia lamblia GL50803_38104   Phospholipid-transporting ATPase IIB, putative
Homo sapiens ENSG00000166377   ATPase, class II, type 9B
Homo sapiens ENSG00000054793   ATPase, class II, type 9A
Leishmania donovani LdBPK_343000.1   aminophospholipid translocase, putative
Leishmania infantum LinJ.34.3000   aminophospholipid translocase, putative
Leishmania major LmjF.34.3220   aminophospholipid translocase, putative
Leishmania mexicana LmxM.33.3220   aminophospholipid translocase, putative
Loa Loa (eye worm) LOAG_13053   transbilayer Amphipath transporters family member
Loa Loa (eye worm) LOAG_02497   transbilayer Amphipath transporters family member
Mus musculus ENSMUSG00000024566   ATPase, class II, type 9B
Mus musculus ENSMUSG00000027546   ATPase, class II, type 9A
Neospora caninum NCLIV_048370   phospholipid-transporting ATPase, P-type, putative
Plasmodium berghei PBANKA_1438300   phospholipid-transporting ATPase, putative
Plasmodium falciparum PF3D7_1223400   phospholipid-transporting ATPase, putative
Plasmodium knowlesi PKNH_1442600   phospholipid-transporting ATPase, putative
Plasmodium vivax PVX_123800   phospholipid-transporting ATPase, putative
Plasmodium yoelii PY06483   similar to ATPase, class II, type 9A
Saccharomyces cerevisiae YIL048W   putative aminophospholipid-translocating P4-type ATPase NEO1
Schistosoma japonicum Sjp_0130010   Probable phospholipid-transporting ATPase IIB, putative
Schistosoma japonicum Sjp_0052350   ko:K01530 phospholipid-translocating ATPase [EC3.6.3.1], putative
Schistosoma japonicum Sjp_0052370   ko:K01530 phospholipid-translocating ATPase [EC3.6.3.1], putative
Schistosoma mansoni Smp_091650   cation-transporting ATPase
Schmidtea mediterranea mk4.001013.07   Putative cation-transporting ATPase
Schmidtea mediterranea mk4.001013.09   Putative cation-transporting ATPase
Schmidtea mediterranea mk4.001013.08  
Schmidtea mediterranea mk4.001013.06   Putative cation-transporting ATPase
Trypanosoma brucei gambiense Tbg972.4.1360   phospholipid-translocating ATPase, putative
Trypanosoma brucei Tb927.4.1510   phospholipid-translocating ATPase, putative
Trypanosoma cruzi TcCLB.511003.10   phospholipid-translocating ATPase, putative
Trypanosoma cruzi TcCLB.504057.11   phospholipid-translocating ATPase, putative
Toxoplasma gondii TGME49_224190   cation-transporting atpase family protein
Theileria parva TP02_0772   phospholipid-transporting ATPase, putative
Trichomonas vaginalis TVAG_119100   cation-transporting ATPase, putative

Essentiality

TGME49_224190 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.4.1510 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.4.1510 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.4.1510 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.4.1510 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F36H2.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_F36H2.1 Caenorhabditis elegans slow growth wormbase
CELE_F36H2.1 Caenorhabditis elegans sterile wormbase
YIL048W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1438300 Plasmodium berghei Slow plasmo
TGME49_224190 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_224190 (Toxoplasma gondii), cation-transporting atpase family protein
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