Detailed view for TGME49_270550

Basic information

TDR Targets ID: 258718
Toxoplasma gondii, gamma-glutamyl phosphate reductase, putative
Source Database / ID:  ToxoDB 

pI: 6.0616 | Length (AA): 502 | MW (Da): 54410 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00171   Aldehyde dehydrogenase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016620   oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor  
GO:0016491   oxidoreductase activity  
GO:0004350   glutamate-5-semialdehyde dehydrogenase activity  
GO:0055114   oxidation reduction  
GO:0008152   metabolic process  
GO:0006561   proline biosynthetic process  

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 493 1t90 (A) 4 480 17.00 0 1 1.02 0.05
45 474 1o20 (A) 2 414 36.00 0 1 1.31 -0.96
46 489 2h5g (A) 362 787 44.00 0 1 1.39 -0.62
11 70 4kna (A) 2 63 12.00 0.015 0.01 0.207322 -0.58
46 489 2h5g (A) 362 787 44.00 0 1 1.31756 0.11

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Tachyzoite, ME49 merozoite. Gregory Hehl AB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Oocyst, ME49 Bradyzoite. Fritz HM Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_127763)

Species Accession Gene Product
Arabidopsis thaliana AT3G55610   delta 1-pyrroline-5-carboxylate synthase 2
Arabidopsis thaliana AT2G39800   delta1-pyrroline-5-carboxylate synthase 1
Candida albicans CaO19.6779   gamma-glutamyl phosphate reductase
Candida albicans CaO19.14071   gamma-glutamyl phosphate reductase
Caenorhabditis elegans CELE_T22H6.2   Protein ALH-13, isoform B
Cryptosporidium hominis Chro.70551   COG0014: gamma-glutamyl phosphate reductase
Cryptosporidium parvum cgd7_4940   gpantothenate kinase 4, rnaseH fold
Drosophila melanogaster Dmel_CG7470   CG7470 gene product from transcript CG7470-RB
Escherichia coli b0243   gamma-glutamylphosphate reductase
Homo sapiens ENSG00000059573   aldehyde dehydrogenase 18 family, member A1
Leishmania braziliensis LbrM.32.3430   pyrroline-5-carboxylate synthetase-like protein
Leishmania donovani LdBPK_323340.1   pyrroline-5-carboxylate synthetase-like protein
Leishmania infantum LinJ.32.3340   pyrroline-5-carboxylate synthetase-like protein
Leishmania major LmjF.32.3140   pyrroline-5-carboxylate synthetase-like protein
Leishmania mexicana LmxM.31.3140   pyrroline-5-carboxylate synthetase-like protein
Mycobacterium leprae ML1458c   PROBABLE GAMMA-GLUTAMYL PHOSPHATE REDUCTASE PROTEIN PROA (GPR) (GLUTAMATE-5-SEMIALDEHYDE DEHYDROGENASE) (GLUTAMYL-GAMMA-SEMIALDE
Mus musculus ENSMUSG00000025007   aldehyde dehydrogenase 18 family, member A1
Mycobacterium tuberculosis Rv2427c   Probable gamma-glutamyl phosphate reductase protein ProA (GPR) (glutamate-5-semialdehyde dehydrogenase) (glutamyl-gamma-semialde
Mycobacterium ulcerans MUL_3696   gamma-glutamyl phosphate reductase
Neospora caninum NCLIV_036210   MGC81784 protein, related
Oryza sativa 4338979   Os05g0455500
Oryza sativa 4324853   Os01g0848200
Saccharomyces cerevisiae YOR323C   glutamate-5-semialdehyde dehydrogenase
Schistosoma japonicum Sjp_0205690   ko:K00147 glutamate-5-semialdehyde dehydrogenase [EC1.2.1.41], putative
Schistosoma mansoni Smp_145020   delta 1-pyrroline-5-carboxylate synthetase
Schmidtea mediterranea mk4.004716.05   Probable delta-1-pyrroline-5-carboxylate synthase
Schmidtea mediterranea mk4.006666.03   Probable delta-1-pyrroline-5-carboxylate synthase
Schmidtea mediterranea mk4.041120.00   Probable delta-1-pyrroline-5-carboxylate synthase
Schmidtea mediterranea mk4.032424.00   Probable delta-1-pyrroline-5-carboxylate synthase
Trypanosoma cruzi TcCLB.511023.10   pyrroline-5-carboxylate synthetase-like protein, putative
Trypanosoma cruzi TcCLB.509067.70   pyrroline-5-carboxylate synthetase-like protein, putative
Toxoplasma gondii TGME49_270550   gamma-glutamyl phosphate reductase, putative
Treponema pallidum TP0350   gamma-glutamyl phosphate reductase

Essentiality

TGME49_270550 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu2469 Mycobacterium tuberculosis non-essential nmpdr
b0243 Escherichia coli non-essential goodall
CELE_T22H6.2 Caenorhabditis elegans slow growth wormbase
TGME49_270550 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.2

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_270550 (Toxoplasma gondii), gamma-glutamyl phosphate reductase, putative
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