Detailed view for TGME49_292080

Basic information

TDR Targets ID: 259349
Toxoplasma gondii, leucyl-tRNA synthetase
Source Database / ID:  ToxoDB 

pI: 6.9463 | Length (AA): 1259 | MW (Da): 141139 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00133   tRNA synthetases class I (I, L, M and V)
PF08264   Anticodon-binding domain of tRNA

Gene Ontology

Mouse over links to read term descriptions.
GO:0002161   GO:aminoacyl-tRNA editing activity  

GO:0005737   cytoplasm  
GO:0005524   ATP binding  
GO:0004823   leucine-tRNA ligase activity  
GO:0004817   cysteine-tRNA ligase activity  
GO:0004812   aminoacyl-tRNA ligase activity  
GO:0000166   nucleotide binding  
GO:0006429   leucyl-tRNA aminoacylation  
GO:0006423   cysteinyl-tRNA aminoacylation  
GO:0006418   tRNA aminoacylation for protein translation  
GO:0006412   translation  

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
113 1257 1wz2 (A) 5 967 31.00 0 1 0.983152 0.91
364 676 2wfd (A) 259 509 51.00 0 1 0.59491 0.16
1088 1245 4uos (A) 26 183 12.00 0.078 0.02 0.283196 -0.61

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_127407)

Species Accession Gene Product
Arabidopsis thaliana AT1G09620   ATP binding/leucine-tRNA ligases/aminoacyl-tRNA ligases
Babesia bovis BBOV_III006860   leucyl-tRNA synthetase, putative
Brugia malayi Bm1_25205   leucyl-tRNA synthetase
Candida albicans CaO19.10094   see CaP19.10092
Candida albicans CaO19.2560   similar to C terminus of S. cerevisiae CDC60 (YPL160W) cytosolic leucine tRNA synthetase
Candida albicans CaO19.2562   see CaP19.2560
Candida albicans CaO19.10092   similar to C terminus of S. cerevisiae CDC60 (YPL160W) cytosolic leucine tRNA synthetase
Caenorhabditis elegans CELE_R74.1   Protein LARS-1
Cryptosporidium hominis Chro.50331   KIAA1352 protein
Cryptosporidium hominis Chro.50330   leucyl tRNA synthetase (134.5 kD) (lrs-1)
Cryptosporidium parvum cgd5_610   conserved hypothetical protein
Dictyostelium discoideum DDB_G0285451   leucine-tRNA ligase
Drosophila melanogaster Dmel_CG33123   CG33123 gene product from transcript CG33123-RB
Echinococcus granulosus EgrG_000104900   leucyl tRNA synthetase
Entamoeba histolytica EHI_161970   leucyl-tRNA synthetase, putative
Echinococcus multilocularis EmuJ_000104900   leucyl tRNA synthetase
Giardia lamblia GL50803_8621   Leucyl-tRNA synthetase
Homo sapiens ENSG00000133706   leucyl-tRNA synthetase
Leishmania braziliensis LbrM.13.0900   leucyl-tRNA synthetase, putative
Leishmania donovani LdBPK_131000.1   leucyl-tRNA synthetase, putative
Leishmania infantum LinJ.13.1000   leucyl-tRNA synthetase, putative
Leishmania major LmjF.13.1100   leucyl-tRNA synthetase, putative
Leishmania mexicana LmxM.13.1100   leucyl-tRNA synthetase, putative
Loa Loa (eye worm) LOAG_07220   hypothetical protein
Loa Loa (eye worm) LOAG_07219   hypothetical protein
Mus musculus ENSMUSG00000024493   leucyl-tRNA synthetase
Neospora caninum NCLIV_012860   Leucyl-tRNA synthetase 2 (EC 6.1.1.4), related
Oryza sativa 4346920   Os09g0378300
Oryza sativa 9272228   Os05g0241100
Oryza sativa 4347497   Os09g0503400
Plasmodium berghei PBANKA_1121700   leucine--tRNA ligase, putative
Plasmodium falciparum PF3D7_0622800   leucine--tRNA ligase, putative
Plasmodium knowlesi PKNH_1127400   leucine--tRNA ligase, putative
Plasmodium vivax PVX_114255   hypothetical protein, conserved
Plasmodium yoelii PY02181   probable leucyl-tRNA synthetase-related
Saccharomyces cerevisiae YPL160W   leucine--tRNA ligase CDC60
Schistosoma japonicum Sjp_0050100   ko:K01869 leucyl-tRNA synthetase [EC6.1.1.4], putative
Schistosoma mansoni Smp_194160   leucyl-tRNA synthetase
Schmidtea mediterranea mk4.001685.01  
Schmidtea mediterranea mk4.001805.12   LeucineALQ-tRNA ligase
Trypanosoma brucei gambiense Tbg972.11.4270  
Trypanosoma brucei Tb927.11.3730   leucyl-tRNA synthetase, putative
Trypanosoma congolense TcIL3000.11.3680   leucyl-tRNA synthetase, putative
Trypanosoma cruzi TcCLB.509679.9   leucyl-tRNA synthetase, putative
Trypanosoma cruzi TcCLB.511049.40   leucyl-tRNA synthetase, putative
Toxoplasma gondii TGME49_292080   leucyl-tRNA synthetase
Theileria parva TP04_0592   leucyl-tRNA synthetase, putative
Trichomonas vaginalis TVAG_334040   leucyl-tRNA synthetase, putative
Trichomonas vaginalis TVAG_217760   leucyl-tRNA synthetase, putative

Essentiality

TGME49_292080 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.02.1210 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.02.1210 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.02.1210 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.02.1210 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_R74.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_R74.1 Caenorhabditis elegans larval arrest wormbase
CELE_R74.1 Caenorhabditis elegans larval lethal wormbase
CELE_R74.1 Caenorhabditis elegans slow growth wormbase
CELE_R74.1 Caenorhabditis elegans sterile wormbase
YPL160W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1121700 Plasmodium berghei Essential plasmo
TGME49_292080 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.7

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens leucyl-tRNA synthetase Compounds References
Saccharomyces cerevisiae leucine--tRNA ligase CDC60 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0297 0.3052 0.4831
0.0265 0.361 0.5883
0.0265 0.361 0.5883
0.0171559 1 1
0.0285 0.3229 0.5287
0.0285 0.3229 0.5287
0.0265 0.361 0.5883
0.0265 0.361 0.5883

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_292080 (Toxoplasma gondii), leucyl-tRNA synthetase
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