Detailed view for TGME49_307040

Basic information

TDR Targets ID: 259618
Toxoplasma gondii, shikimate dehydrogenase substrate binding domain-containing protein

Source Database / ID:  ToxoDB 

pI: 6.9881 | Length (AA): 3215 | MW (Da): 349119 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00275   EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
PF01202   Shikimate kinase
PF01487   Type I 3-dehydroquinase
PF01761   3-dehydroquinate synthase
PF08501   Shikimate dehydrogenase substrate binding domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0016765   transferase activity, transferring alkyl or aryl (other than methyl) groups  
GO:0005524   ATP binding  
GO:0005488   binding  
GO:0004765   shikimate kinase activity  
GO:0004764   shikimate 5-dehydrogenase activity  
GO:0003856   3-dehydroquinate synthase activity  
GO:0003855   3-dehydroquinate dehydratase activity  
GO:0003824   catalytic activity  
GO:0055114   oxidation reduction  
GO:0009073   aromatic amino acid family biosynthetic process  
GO:0008152   metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 24 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
236 500 1o2d (A) 4 288 13.00 0 0.33 0.07 -0.41
325 698 1sg6 (A) 102 392 39.00 0 1 0.2 -0.06
723 1198 1rf6 (A) 5 424 14.00 0 1 0.18 0.62
1220 1665 2aa9 (A) 2 425 13.00 0 0.96 0 0.47
2932 3256 1npy (A) 1 269 25.00 0 1 -0.02 0.07
2937 3323 1gpj (A) 46 374 15.00 0 0.95 -0.26 0.67
2945 3249 1vi2 (A) 10 282 21.00 0 1 0.27 -0.23
227 698 3okf (A) 5 361 27.00 0 1 0.0974118 0.85
321 697 3okf (A) 92 360 37.00 0 1 0.0895628 0.6
327 663 5eks (A) 93 322 44.00 0 1 0.0374212 1.2
526 697 4p53 (A) 220 391 24.00 0 1 0.361799 -0.32
718 1279 3nvs (A) 2 423 21.00 0 1 0.0124056 1.18
727 1033 2aa9 (A) 11 199 42.00 0.00000038 1 -0.10091 0.89
733 1033 3ti2 (A) 17 200 39.00 0.0093 1 -0.125776 0.72
1233 1284 3nvs (A) 237 288 44.00 0.00011 0.5 0.397774 1.01
1468 1668 3sg1 (A) 233 414 13.00 0 0.57 0.117819 -0.16
1486 1668 3slh (A) 298 429 33.00 0 0.99 -0.00877932 0.47
1530 1668 5buf (A) 594 752 31.00 0.29 0.72 0.232835 -0.05
1996 2118 1kag (A) 26 162 20.00 0 0.66 0.227558 -0.26
2383 2450 2ocz (A) 20 87 32.00 0 0.46 0.474451 -1.15
2389 2452 2egz (A) 24 88 30.00 0 0.61 0.427207 -1.22
2984 3135 1npy (A) 47 265 32.00 0 0.92 -0.0654216 0.27
2984 3128 3phh (A) 48 253 30.00 0 1 -0.0725989 0.4
2991 3050 3u62 (A) 50 102 40.00 0.52 0.82 0.285263 0.78

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_127183)

Species Accession Gene Product
Arabidopsis thaliana AT2G45300   3-phosphoshikimate 1-carboxyvinyltransferase / 5-enolpyruvylshikimate-3-phosphate / EPSP synthase
Arabidopsis thaliana AT1G48860   putative 5-enolpyruvylshikimate-3-phosphate synthase
Arabidopsis thaliana AT5G66120   putative 3-dehydroquinate synthase
Candida albicans CaO19.12175   similar to S. cerevisiae ARO1 pentafunctional aromatic amino acid family biosynthesis protein
Candida albicans CaO19.4704   similar to S. cerevisiae ARO1 pentafunctional aromatic amino acid family biosynthesis protein
Chlamydia trachomatis CT_366   phosphoshikimate 1-carboxyl vinyltransferase
Chlamydia trachomatis CT_369   dehyroquinate synthase
Escherichia coli b3389   3-dehydroquinate synthase
Escherichia coli b0908   5-enolpyruvylshikimate-3-phosphate synthetase
Mycobacterium leprae ML0792c   probable 3-phosphoshikimate 1-carboxyvinyl transferase AroA (5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE) (EPSP SYNTHASE) (EPSPS
Mycobacterium leprae ML0518   3-dehydroquinate synthase AroB
Mycobacterium tuberculosis Rv3227   3-phosphoshikimate 1-carboxyvinyltransferase AroA (5-enolpyruvylshikimate-3-phosphate synthase) (EPSP synthase) (EPSPS)
Mycobacterium tuberculosis Rv2538c   3-dehydroquinate synthase AroB
Mycobacterium ulcerans MUL_2560   3-phosphoshikimate 1-carboxyvinyltransferase
Mycobacterium ulcerans MUL_1761   3-dehydroquinate synthase
Neospora caninum NCLIV_053120   3-dehydroquinate synthase (EC 4.2.3.4), related
Oryza sativa 4347717   Os09g0539100
Oryza sativa 4340026   Os06g0133900
Saccharomyces cerevisiae YDR127W   pentafunctional protein ARO1p
Schistosoma mansoni Smp_109520   3-dehydroquinate synthase
Schmidtea mediterranea mk4.065648.00  
Toxoplasma gondii TGME49_307040   shikimate dehydrogenase substrate binding domain-containing protein

Essentiality

TGME49_307040 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu2582 Mycobacterium tuberculosis essential nmpdr
b0908 Escherichia coli non-essential goodall
b3389 Escherichia coli non-essential goodall
TGME49_307040 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.


Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6


Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Escherichia coli 5-enolpyruvylshikimate-3-phosphate synthetase Compounds References
Escherichia coli 3-dehydroquinate synthase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model

Ranking Plot


Putative Drugs List


Compound Raw Global Species
0.0564 0.2793 1
0.0509 0.2731 1
0.1865 0.2881 0.2881
0.0383 0.2761 1
0.0397 0.2768 1
0.0177 0.2606 1
0.0568 0.2792 1
0.035 0.2758 1
0.0362 0.2756 1
0.0529 0.2779 1
0.014 0.5051 0.8742
0.0218 0.2641 1
0.0573 0.2784 1
0.0631 0.2783 1
0.1872 1 1
0.0508 1 1
0.0403 0.2775 1
0.0356 0.2782 1
0.0523 0.2748 0.9082
0.0487 0.344 1
0.0258 1 0.5
0.2044 0.4186 0.7774
0.0578 0.5361 0.5325
0.0176 0.4748 1
0.0595 0.2774 1
0.0355 0.2762 1
0.0528 1 1
0.0617 1 1
0.0551 0.2779 1
0.0561 0.2692 1
0.0357 1 0.5
0.0632 0.2774 1
0.0545 0.271 0.4171
0.0432 0.5954 1
0.0286 1 1
0.0599 0.2784 1
0.0625 0.2774 1
0.0554 0.277 1
0.0387 0.2737 1
0.0376 0.2769 1
0.2044 0.4186 0.7774
0.0523 0.2748 0.9082
0.0555 0.2769 1
0.0377 0.2766 1
0.0629 0.2774 1
0.0364 0.2789 1
0.2044 0.4186 0.7774
0.0543 0.2753 0.948
0.0356 0.2701 1
0.0356 0.2772 1
0.0551 0.2726 1
0.2024 0.5934 1
0.0353 0.2766 1
0.0258 1 0.5
0.035 0.2558 1
0.0416 0.2774 1
0.0543 0.2791 1
0.0213 0.379 1
0.0277 1 1
0.0394 0.2791 1
0.0629 0.2777 1
0.0383 0.2766 1
0.0365 0.2746 1
0.0559 0.2767 1
0.0357 0.2889 1
0.0543 0.2712 1
0.0563 0.2773 1
0.0357 0.2975 1
0.0608 0.2787 1
0.0291 0.2846 0.8146
0.0383 0.2774 1
0.2044 0.4186 0.7774
0.0367 0.2745 1
0.1438 0.9306 0.9306
0.0397 0.2773 1
0.0647 0.2775 1
0.0364 0.2756 1
0.0374 0.2762 1
0.039 1 1
0.0357 0.9002 1
0.029 0.2739 1
0.0609 0.279 1
0.0389 0.2771 1
0.0602 0.2782 1
0.0353 0.2744 1
0.1839 0.2879 0.2879
0.0548 0.2782 1
0.1997 0.705 1
0.0558 0.2712 1
0.0504 0.2918 1
0.0351 0.2706 1
0.0563 0.2773 1
0.0376 0.2671 0.5
0.2044 0.4186 0.7774
0.029 1 1
0.0541 0.2778 1
0.0685 1 0.5
0.0594 0.2766 1
0.0622 0.2773 1
0.2044 0.4186 0.7774
0.0426 0.2744 0.9175
0.0668 1 0.5
0.0357 0.2745 1
0.055 0.2756 1
0.0602 0.2781 1
0.0542 1 0.5
0.0357 0.2975 1
0.2044 0.4186 0.7774
0.0176 0.2597 1
0.0354 0.2662 0.5
0.0349 0.2755 1
0.0174 0.2664 1
0.0357 0.2729 1
0.0344 0.2693 1
0.0176 0.2711 1
0.0288 0.3553 0.3931
0.0218 0.2643 0.3753
0.0588 0.2764 1
0.2044 0.4186 0.7774
0.0357 0.2771 1
0.0558 0.2774 1
0.0601 0.279 1
0.056 0.2744 0.9632
0.0616 0.2792 1
0.0371 0.2764 1
0.0264 0.3965 1
0.0379 0.2769 1
0.0404 0.2777 1
0.0538 0.2763 1
0.0178 1 1
0.0368 0.2765 1
0.0622 0.2773 1
0.0258 0.2871 0.4959
0.0602 0.2772 1
0.0369 0.2716 1
0.0543 0.2711 1
0.0371 0.2764 1
0.1865 0.2881 0.2881
0.0375 0.2767 1
0.0609 0.2778 1
0.0387 0.2747 1
0.0573 0.2682 1
0.014 0.5051 0.8742
0.2044 0.4186 0.7774
0.0548 0.2771 1
0.1655 0.2877 0.2877
0.0349 0.2755 1
0.0651 0.2913 0.2913
0.1644 0.5016 0.5354
0.0397 0.2721 1
0.0609 0.2777 1
0.0361 0.274 1
0.0537 0.2776 1
0.0362 0.2745 1
0.0361 0.274 1
0.1625 1 1
0.0354 0.2708 1
0.0388 0.2769 1
0.0639 0.2778 1
0.0356 0.2702 0.5
0.0601 0.2779 1
0.0563 0.2678 1
0.0558 0.2774 1
0.1016 1 0.5
0.056 0.2766 1
0.0541 0.2765 1
0.0563 0.2711 1
0.0747 0.7303 1
0.0436 0.2731 1
0.0585 0.2791 1
0.0294 1 0.5
0.0396 0.2766 1
0.0294 0.2703 0.2619
0.0551 0.2779 1
0.0528 0.2765 1
0.0378 0.2766 1
0.0377 0.2634 0.5
0.0207 0.2636 1
0.0402 0.2766 1
0.0391 0.2739 1
0.2181 0.9004 1
0.0357 0.2764 1
0.0258 1 1
0.0595 0.2774 1
0.0199 0.2569 1
0.0385 0.2734 1
0.3103 1 1
0.0593 0.2794 1
0.0642 0.2892 1
0.0353 0.271 1
0.0359 0.271 1
0.0689 0.3007 1
0.03 1 1
0.0599 0.2782 1
0.0563 0.2773 1
0.0356 0.277 1
0.0351 0.2706 1
0.0543 0.2748 1
0.055 0.2757 1
0.043 0.278 1
0.0408 0.2791 1
0.0349 0.2755 1
0.1786 0.7732 0.8432
0.0561 0.2692 1
0.038 1 0.5
0.0558 0.2774 1
0.0397 0.2739 1
0.0391 0.2778 1
0.0356 0.2766 1
0.0614 0.2781 1
0.0423 0.2772 1
0.0423 0.2776 1
0.0487 0.344 1
0.0357 0.2754 1
0.0551 0.2779 1
0.0377 0.4519 0.6821
0.0609 0.2778 1
0.0563 0.2773 1
0.0542 0.2787 1
0.0532 0.272 1
0.0344 0.2693 1
0.0357 0.2975 1
0.0934 1 1
0.2044 0.4186 0.7774
0.0353 0.271 1
0.0294 0.2854 0.3896
0.0571 0.2786 1
0.0523 0.2748 0.9082
0.0357 0.2754 1
0.0377 0.2766 1
0.0461 1 1
0.0587 0.2794 0.9915
0.0349 0.2755 1
0.0396 0.2775 1
0.0383 0.2739 1
0.0176 0.2597 1
0.0545 0.271 0.4171
0.055 0.2709 0.7064
0.0456 0.462 1
0.0207 0.2636 1
0.3103 1 1
0.0409 1 1
0.056 0.2744 0.9632
0.0362 0.2701 1
0.0174 0.2664 1
0.0357 0.9074 1
0.0368 0.274 1
0.0146 0.2535 0.5043
0.0527 0.2712 1
0.0387 0.2747 1
0.0357 0.3658 1
0.0549 0.2782 1
0.0352 0.2703 1
0.0374 0.2762 1
0.0637 0.2774 1
0.0523 0.2712 0.8816
0.0356 0.277 1
0.0389 0.2766 1
0.0391 0.2766 1
0.0528 0.2777 1
0.0546 0.2773 1
0.0177 0.2705 1
0.2044 0.4186 0.7774
0.0629 0.2777 1
0.0581 0.2779 1
0.0357 0.2727 1
0.1771 0.344 0.3466
0.0672 0.2686 1
0.0356 0.2782 1
0.0631 0.281 0.2716
0.06 0.2776 1
0.0537 0.2776 1
0.0566 0.2797 1
0.0357 0.2534 0.5
0.0378 0.2764 1
0.0541 0.2676 1
0.0383 0.2739 1
0.0369 0.2793 1
0.0369 0.2766 1
0.0528 0.2712 0.9956
0.0618 1 0.5
0.0357 0.2889 1
0.0536 0.2764 1
0.0689 0.3007 1
0.0361 0.274 1
0.0369 0.2764 1
0.0174 0.2664 1
0.0537 0.2785 1
0.0357 0.2534 0.5
0.0622 0.2773 1
0.0361 0.277 1
0.0258 0.2871 0.4959
0.0383 0.2761 1
0.0383 0.2763 1
0.0403 0.2739 1
0.0287 0.3026 0.3026
0.0747 0.7303 1
0.0411 0.2765 1
0.0493 0.9356 0.5
0.0392 0.2775 1
0.029 0.2739 1
0.0529 0.2779 1
0.0386 0.2771 1
0.0622 0.2772 1
0.0359 0.2791 1
0.0578 0.277 1
0.0396 0.3646 1
0.0535 0.2781 1
0.0397 0.2739 1
0.055 0.2757 1
0.1222 1 0.5
0.041 0.2772 1
0.0307 1 0.5
0.2044 0.4186 0.7774
0.035 0.2758 1
0.0374 0.2613 1
0.0566 0.2678 1
0.0374 0.2745 1
0.0367 0.2764 1
0.1839 0.5934 1
0.0536 0.2777 1
0.0571 0.2794 1
0.041 0.277 1
0.0436 0.2731 1
0.0504 0.2918 1
0.014682 0.3075 0.5
0.037 0.2791 1
0.2278 0.3467 0.3467
0.0354 0.2662 0.5
0.0362 0.2599 1
0.0408 0.2731 1
0.0668 0.4992 0.5
0.0352 0.2708 1
0.0378 0.2766 1
0.033 0.27 1
0.0403 0.2739 1
0.0532 0.2711 1
0.054 0.2694 1
0.0383 0.2766 1
0.0383 0.2739 1
0.0668 0.4992 0.5
0.0543 0.2791 1
0.0349 0.2764 1
0.1997 0.705 1
0.0357 0.5171 0.5171
0.2044 0.4186 0.7774
0.1625 1 1
0.0562 0.269 1
0.0371 0.2671 1
0.0543 0.2711 1
0.0371 0.2713 1
0.0378 0.2766 1
0.0432 0.5954 1
0.035 0.2558 1
0.0366 0.2764 1
0.043 0.278 1
0.0349 0.2721 1
0.0594 0.2779 1
0.0357 0.2975 1
0.0528 0.2717 1
0.0558 0.2774 1
0.1813 0.9107 1
0.0307 1 0.5
0.0357 0.9002 1
0.0546 0.2693 1
0.0285 0.2746 1
0.0617 1 1
0.041 0.277 1
0.0383 0.2713 1
0.0532 0.2803 1
0.0534 0.2784 1
0.0526 0.2774 1
0.0349 0.2745 1
0.0575 0.2778 1
0.0764 0.4585 1
0.041 0.2771 1
0.0539 0.278 1
0.0535 0.2712 1
0.0538 0.2711 1
0.0543 0.2761 1
0.0218 0.2643 0.3753
0.0352 0.273 1
0.0637 0.2773 1
0.0403 0.2759 1
0.0287 1 0.5
0.0566 0.2797 1
0.0376 0.2709 1
0.0362 0.276 1
0.0526 0.2774 1
0.0548 0.2782 1
0.0365 0.2714 1
0.187 1 1
0.0542 0.2767 1
0.2044 0.4186 0.7774
0.0361 0.2745 1
0.2049 0.5935 1
0.0307 1 0.5
0.187 1 1
0.0523 0.2686 1
0.0578 0.2793 1
0.0371 0.2713 1
0.0578 0.2792 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_307040 (Toxoplasma gondii), shikimate dehydrogenase substrate binding domain-containing protein
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