Detailed view for TGME49_248400

Basic information

TDR Targets ID: 259990
Toxoplasma gondii, glyoxalase I, putative
Source Database / ID:  ToxoDB 

pI: 6.2963 | Length (AA): 336 | MW (Da): 38472 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00903   Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily

Gene Ontology

Mouse over links to read term descriptions.
GO:0046872   metal ion binding  
GO:0004462   lactoylglutathione lyase activity  

Metabolic Pathways

Pyruvate metabolism (KEGG)

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 173 1qip (A) 11 179 41.00 0 1 0.9 -0.09
3 173 1qip (A) 14 179 45.00 0 1 1.05 -0.33
27 326 5d7z (A) 31 280 33.00 0 1 1.06856 0.08
117 167 2p25 (A) 73 124 29.00 0.32 0.99 0.598486 -0.74
254 329 3w0t (A) 102 177 54.00 0.0000000071 1 0.85479 -0.58
288 335 1zsw (A) 235 281 15.00 0.069 0.23 0.143457 0.83

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Hehl AB Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite. Gregory
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_127627)

Species Accession Gene Product
Arabidopsis thaliana AT1G08110   lactoylglutathione lyase
Brugia malayi Bm1_18670   lactoylglutathione lyase
Candida albicans CaO19.6058   glyoxalase I
Candida albicans CaO19.13479   glyoxalase I
Dictyostelium discoideum DDB_G0291265   glyoxylase I
Drosophila melanogaster Dmel_CG1707   CG1707 gene product from transcript CG1707-RA
Escherichia coli b1651   glyoxalase I, Ni-dependent
Homo sapiens ENSG00000124767   glyoxalase I
Leishmania braziliensis LbrM.34.2920   glyoxalase I
Leishmania donovani LdBPK_353060.1   glyoxalase I
Leishmania infantum LinJ.35.3060   glyoxalase I,trypanothione-dependent glyoxalase I
Leishmania major LmjF.35.3010   glyoxalase I,trypanothione-dependent glyoxalase I
Leishmania mexicana LmxM.34.3010   glyoxalase I,trypanothione-dependent glyoxalase I
Loa Loa (eye worm) LOAG_03565   hypothetical protein
Mus musculus ENSMUSG00000024026   glyoxalase 1
Neospora caninum NCLIV_064370   Lactoylglutathione lyase (EC 4.4.1.5), related
Oryza sativa 4338324   Os05g0295800
Onchocerca volvulus OVOC11536   Lactoylglutathione lyase homolog
Plasmodium berghei PBANKA_0933900   glyoxalase I, putative
Plasmodium falciparum PF3D7_1113700   glyoxalase I
Plasmodium knowlesi PKNH_0911300   glyoxalase I, putative
Plasmodium vivax PVX_091330   glyoxalase I, putative
Plasmodium yoelii PY00733   lactoylglutathione lyase, putative
Saccharomyces cerevisiae YML004C   lactoylglutathione lyase GLO1
Trypanosoma cruzi TcCLB.510743.70   lactoylglutathione lyase-like protein, putative
Trypanosoma cruzi TcCLB.510659.240   lactoylglutathione lyase-like protein, putative
Toxoplasma gondii TGME49_248400   glyoxalase I, putative

Essentiality

TGME49_248400 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
b1651 Escherichia coli non-essential goodall
PBANKA_0933900 Plasmodium berghei Dispensable plasmo
TGME49_248400 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.6

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Leishmania major glyoxalase I,trypanothione-dependent glyoxalase I Compounds References
Plasmodium falciparum glyoxalase I Compounds References
Saccharomyces cerevisiae lactoylglutathione lyase GLO1 Compounds References
Trypanosoma cruzi lactoylglutathione lyase-like protein, putative Compounds References
Homo sapiens glyoxalase I Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.1277 1 1
0.108 1 1
0.0945 1 1
0.0198 0.3331 1
0.0629 0.8426 1
0.1179 1 1
0.1452 1 1
0.1213 1 1
0.1292 0.8444 1
0.0187 0.646 1
0.0985 1 1
0.0835 0.3161 1
0.1139 1 1
0.0186 0.5889 1
0.1323 0.5949 0.5947
0.0393 0.2815 1
0.1306 1 1
0.1581 0.3867 1
0.0774 1 1
0.0384846 1 1
0.0966127 1 1
0.0465 0.4779 1
0.0399 0.7908 1
0.108 1 1
0.0188 0.3141 1
0.038 0.2886 1
0.0775 0.9315 1
0.0843 0.2609 1
0.0654 0.367 1
0.3128 1 1
0.1055 1 1
0.1333 1 1
0.0829 1 1
0.0232 0.8164 1
0.2445 1 1
0.0384 0.8718 1
0.0465 0.5 0.5
0.0602 0.7403 1
0.2541 1 1
0.0992 1 1
0.2261 0.8461 0.8663
0.137 0.5579 1
0.1028 1 1
0.2991 1 1
0.0751 1 1
0.0929 0.7062 1
0.1251 0.7189 1
0.1323 1 1
0.039 1 1
0.0232 0.2903 1
0.0796 0.3036 1
0.0198 0.7702 1
0.1365 1 1
0.0991 1 1
0.1088 1 1
0.0761 1 1
0.0775 0.9315 1
0.2907 1 1
0.0966 0.3695 1
0.1294 0.2596 1
0.133 0.3224 1
0.0216 0.2524 1
0.1323 0.5949 0.5947
0.0187 0.7073 1
0.0186 0.5889 1
0.0465 0.4779 1
0.1403 1 1
0.0801 0.3037 1
0.0218 1 1
0.0443 1 1
0.1251 0.7189 1
0.0603 1 1
0.0602 0.7403 1
0.0374 0.3862 1
0.0431 1 1
0.0835 0.3161 1
0.1583 1 1
0.2991 1 1
0.0788 1 1
0.0358 0.2883 1
0.0208 1 1
0.1059 1 1
0.0629 0.8426 1
0.108 1 1
0.1251 0.7189 1
0.0403 0.991 1
0.0835 0.3161 1
0.0354 1 1
0.0806 1 1
0.0835 0.3161 1
0.0838 1 1
0.1573 1 1
0.1202 1 1
0.0801 0.3037 1
0.0595 0.4345 1
0.0187 0.5857 1
0.0111 0.2716 0.4766
0.0992 1 1
0.0929 0.7062 1
0.108 1 1
0.0755 1 1
0.2991 1 1
0.1334 1 1
0.1264 1 1
0.0431 0.5 0.5
0.0378 0.432 1
0.0384846 1 1
0.0405 0.2786 0.7612
0.0291 1 1
0.0221 1 1
0.0844 0.3778 1
0.0779 1 1
0.0582 0.6925 1
0.2671 1 1
0.1349 0.5571 1
0.0622 0.2734 1
0.0612 0.3486 1
0.0384 0.8718 1
0.1581 0.3867 1
0.0232 1 1
0.133 0.3224 1
0.1581 0.3867 1
0.0605 0.3168 0.4278
0.0465 0.4779 1
0.139721 1 1
0.0997 1 1
0.0402 0.4871 1
0.0835 0.3161 1
0.0835 1 1
0.0187 0.5857 1
0.082 0.7919 1
0.0596 1 1
0.0232 0.4317 1
0.0378 1 1
0.0379 0.5 0.5
0.0787 0.9548 1
0.0835 0.3161 1
0.0561 0.3039 1
0.1071 1 1
0.0196 0.4171 1
0.0187 0.4339 1
0.1581 0.3867 1
0.108 1 1
0.0605 0.3168 0.4278
0.1334 1 1
0.0761 1 1
0.1292 0.8444 1
0.1334 1 1
0.1323 0.5949 0.5947
0.1459 1 1
0.108 1 1
0.1403 1 1
0.1323 0.5949 0.5947
0.0196 1 1
0.0232 0.7063 1
0.0605 0.3168 0.4278
0.108 1 1
0.0232 1 1
0.0813 1 1
0.0788 1 1
0.02 0.392 1
0.0194 0.3716 0.5
0.1037 1 1
0.0465 0.4779 1
0.0952 1 1
0.0385 0.5491 1
0.1055 1 1
0.106 1 0.5
0.0755 1 1
0.019 0.4043 1
0.0232 1 1
0.1349 0.5571 1
0.0443 1 1
0.0232 1 1
0.0629 0.8426 1
0.1283 1 1
0.0189 0.3189 0.9008
0.0198 0.6479 1
0.0403 0.991 1
0.0929 0.4041 1
0.1148 0.5577 1
0.0382 0.4516 1
0.0186 0.271 1
0.1306 1 1
0.0602 0.7403 1
0.0405 0.2786 0.7612
0.1574 1 1
0.2657 1 1
0.0386 0.5416 1
0.0358 0.2883 1
0.069 0.9317 1
0.0232 1 1
0.1592 1 1
0.0189 1 0.5
0.0232 0.29 0.2898
0.0387 0.4194 1
0.1377 0.3339 1
0.019 0.6467 1
0.0203 0.648 1
0.1337 0.5454 1
0.1585 1 1
0.0443 1 1
0.1295 1 1
0.0929 0.7062 1
0.1426 1 1
0.0199 0.6479 1
0.0814 1 1
0.0251 0.4658 0.8694
0.2991 1 1
0.0391 0.4757 1
0.0196 0.3529 0.5
0.0835 0.3161 1
0.0813 1 1
0.1294 0.2596 1
0.1046 1 1
0.0577 1 1
0.0205 1 1
0.0232 1 1
0.1294 0.2596 1
0.108 1 1
0.0966 0.3695 1
0.0393 0.3045 1
0.0465 0.5 0.5
0.1071 1 1
0.08 0.356 1
0.0806 1 1
0.0818 1 1
0.1251 0.7189 1
0.3063 1 1

Assayability

Assay information

  • Assay for Glyoxalase I (4.4.1.5 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_248400 (Toxoplasma gondii), glyoxalase I, putative
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