Detailed view for TGME49_234570

Basic information

TDR Targets ID: 260127
Toxoplasma gondii, sterol carrier protein-2 HAD-2SCP-2
Source Database / ID:  ToxoDB 

pI: 9.7876 | Length (AA): 625 | MW (Da): 67508 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00106   short chain dehydrogenase
PF02036   SCP-2 sterol transfer family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016491   oxidoreductase activity  
GO:0005498   sterol carrier activity  
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Metabolic Pathways

Global map (KEGG)
Peroxisome (KEGG)

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 297 1gz6 (A) 4 301 53.00 0 1 1.0894 -0.12
2 278 2et6 (A) 3 281 55.00 0 1 1.0665 -0.26
3 487 3oml (A) 8 569 35.00 0 1 0.9003 1.03
3 360 3oml (A) 8 351 48.00 0 1 0.8856 0.71
340 451 1c44 (A) 12 123 28.00 0 1 0.6069 -1.15
500 625 1c44 (A) 1 123 33.00 0 1 0.6749 -0.96
505 622 1pz4 (A) 2 110 21.00 0.00000001 0.96 0.4251 -0.23

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 merozoite, ME49 Oocyst. Hehl AB Fritz HM
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_129142)

Species Accession Gene Product
Arabidopsis thaliana AT1G76150   enoyl-CoA hydratase 2
Brugia malayi Bm1_44740   oxidoreductase, short chain dehydrogenase/reductase family protein
Brugia malayi Bm1_24050   maoC like domain containing protein
Candida albicans CaO19.1809   similar to C.tropicalis HDE1 (P22414) peroxisomal trifunctional hydratase-dehydrogenase-epimerase important in fatty acid beta-o
Caenorhabditis elegans CELE_E04F6.3   Protein MAOC-1
Caenorhabditis elegans CELE_M03A8.1   Protein DHS-28
Dictyostelium discoideum DDB_G0291247   hypothetical protein
Dictyostelium discoideum DDB_G0277911   hypothetical protein
Drosophila melanogaster Dmel_CG3415   peroxisomal Multifunctional enzyme type 2
Homo sapiens ENSG00000133835   hydroxysteroid (17-beta) dehydrogenase 4
Loa Loa (eye worm) LOAG_08780   short chain dehydrogenase/reductase family oxidoreductase
Loa Loa (eye worm) LOAG_00696   hypothetical protein
Mus musculus ENSMUSG00000024507   hydroxysteroid (17-beta) dehydrogenase 4
Mycobacterium tuberculosis Rv0148   Probable short-chain type dehydrogenase/reductase
Mycobacterium tuberculosis Rv3389c   Probable 3-hydroxyacyl-thioester dehydratase HtdY
Mycobacterium tuberculosis Rv3538   Probable dehydrogenase. Possible 2-enoyl acyl-CoA hydratase.
Mycobacterium ulcerans MUL_4099   dehydratase
Mycobacterium ulcerans MUL_0920   hypothetical protein
Mycobacterium ulcerans MUL_4759   short-chain type dehydrogenase/reductase
Mycobacterium ulcerans MUL_4812   short-chain type dehydrogenase/reductase
Neospora caninum NCLIV_042220   peroxisomal multifunctional enzyme type 2, putative
Neospora caninum NCLIV_030080   peroxisomal multifunctional enzyme, putative
Oryza sativa 4347752   Os09g0544900
Saccharomyces cerevisiae YKR009C   bifunctional hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase FOX2
Schmidtea mediterranea mk4.001730.03  
Schmidtea mediterranea mk4.007769.01   Peroxisomal multifunctional enzyme type 2
Toxoplasma gondii TGME49_234570   sterol carrier protein-2 HAD-2SCP-2
Toxoplasma gondii TGME49_229140   MaoC family domain-containing protein

Essentiality

TGME49_234570 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu149 Mycobacterium tuberculosis non-essential nmpdr
mtu3449 Mycobacterium tuberculosis non-essential nmpdr
mtu3599 Mycobacterium tuberculosis essential nmpdr
CELE_M03A8.1 Caenorhabditis elegans larval arrest wormbase
CELE_M03A8.1 Caenorhabditis elegans slow growth wormbase
CELE_E04F6.3 Caenorhabditis elegans larval arrest wormbase
TGME49_229140 Toxoplasma gondii Essentiality uncertain sidik
TGME49_234570 this record Toxoplasma gondii Essentiality uncertain sidik
TGME49_229140 Toxoplasma gondii Probably non-essential sidik
TGME49_234570 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.4

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Homo sapiens hydroxysteroid (17-beta) dehydrogenase 4 Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Plasmodium falciparum 3-oxoacyl-[acyl-carrier-protein] reductase 301 aa 35.6% 250 aa Compounds References
Leishmania major pteridine reductase 1 288 aa 25.5% 278 aa Compounds References
Macaca mulatta Hydroxysteroid 11-beta dehydrogenase 1 140 aa 31.4% 153 aa Compounds References
Cochliobolus lunatus 17-beta-hydroxysteroid-dehydrogenase 270 aa 29.4% 269 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0088 0.6544 0.5
0.0056 0.875 0.5
0.0218 0.5 0.5
0.007 1 0.5
0.0132 0.3658 1
0.0073 0.3141 1
0.0097 1 1
0.0035 1 0.5
0.0073 0.2873 0.7466
0.0021 0.5 0.5
0.0004 0.5 0.5
0.0162 1 1
0.0114 1 0.5
0.0009 0.5 0.5
0.0012 0.5 0.5
0.0171 0.4063 0.5
0.0165 1 1
0.0118 1 1
0.007 0.2916 1
0.0004 0.5 0.5
0.0043 0.3113 1
0.0077 0.3481 1
0.0057 0.284 0.6352
0.0056 1 0.5
0.0082 1 0.5
0.004 0.5 0.5
0.0196 0.8679 1
0.0073 0.3811 1
0.0097 0.4304 0.5
0.0012 0.5 0.5
0.016 0.577 1
0.0073 0.3811 1
0.008 0.5 0.5
0.0021 0.3321 0.5
0.0004 0.5 0.5
0.0009 0.5 0.5
0.0119 0.2746 1
0.0196 0.8679 1
0.0114 1 0.5
0.0004 0.5 0.5
0.0108 0.5 0.5
0.007 0.2916 1
0.0089 0.5 0.5
0.0013 1 0.5
0.0016 0.5 0.5
0.0402 1 1
0.0019 0.4322 1
0.0068 1 1
0.007 1 0.5
0.0004 0.5 0.5
0.0067 0.3468 1
0.0106 0.2872 1
0.0106 1 0.5
0.0123 0.5 0.5
0.0196 0.8679 1
0.0058 1 1
0.0009 0.7374 0.5
0.0113 1 1
0.0009 0.5 0.5
0.0009 1 0.5
0.0009 0.5 0.5
0.0005 0.5 0.5
0.0088 1 0.5
0.0058 0.3931 0.5
0.0026 0.5 0.5
0.016 0.577 1
0.0089 0.5 0.5
0.007 0.2916 1
0.0085 1 1
0.0084 0.4283 0.4047
0.0013 0.5 0.5
0.0053 1 1
0.0005 0.5 0.5
0.0068 0.2585 0.5
0.0157 0.5 0.5
0.0222 0.5 0.5
0.0071 0.3141 0
0.0066 0.3586 0.5
0.011 0.3568 1
0.0087 0.5 0.5
0.0408 0.5144 1
0.0009 0.5 0.5
0.0032 1 1
0.0102 0.7451 0.5
0.0064 0.3508 0.5
0.0076 1 0.5
0.0027 0.5 0.5
0.0131 0.5 0.5
0.0021 0.3321 0.5
0.0408 0.5144 1
0.0004 0.5 0.5
0.0093 0.5 0.5
0.0106 0.5 0.5
0.007 1 0.5
0.0053 1 1
0.0004 0.5 0.5
0.0106 1 1
0.0027 0.5 0.5
0.0039 1 0.5
0.0045 1 1
0.0009 0.5 0.5
0.0009 0.5 0.5
0.0046 0.3082 0
0.0068 1 1
0.0009 0.5 0.5
0.0171 0.4063 0.5
0.0073 0.4546 1
0.0408 0.5144 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

3 literature references were collected for this gene.

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Gene identifier TGME49_234570 (Toxoplasma gondii), sterol carrier protein-2 HAD-2SCP-2
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