Detailed view for TGME49_255890

Basic information

TDR Targets ID: 260301
Toxoplasma gondii, pyridine nucleotide-disulfide oxidoreductase domain-containing protein
Source Database / ID:  ToxoDB 

pI: 5.852 | Length (AA): 788 | MW (Da): 85898 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF07992   Pyridine nucleotide-disulphide oxidoreductase

Gene Ontology

Mouse over links to read term descriptions.
GO:0050660   FAD binding  
GO:0016491   oxidoreductase activity  
GO:0055114   oxidation reduction  

Metabolic Pathways

Ascorbate and aldarate metabolism (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 532 1gte (A) 27 499 16.00 0 0.9 0.31 0.88
159 717 2cdu (A) 2 450 15.00 0 1 0.31 0.54
350 548 2cvj (A) 3 174 19.00 0 1 0.17 -0.73
226 768 3lxd (A) 8 411 25.00 0.063 1 0.578286 0.98
266 749 5jci (A) 37 412 38.00 0 1 0.709113 0.7
406 530 5u1o (A) 160 275 28.00 0.24 1 0.520529 -0.57

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite, ME49 Bradyzoite. Hehl AB Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Oocyst. Gregory Fritz HM
Show/Hide expression data references
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_126784)

Species Accession Gene Product
Arabidopsis thaliana AT3G27820   monodehydroascorbate reductase (NADH)
Arabidopsis thaliana AT3G52880   monodehydroascorbate reductase 1
Arabidopsis thaliana AT3G09940   monodehydroascorbate reductase (NADH)
Arabidopsis thaliana AT1G63940   Monodehydroascorbate reductase
Arabidopsis thaliana AT5G03630   monodehydroascorbate reductase (NADH)
Babesia bovis BBOV_III001680   pyridine nucleotide-disulphide oxidoreductase domain containing protein
Caenorhabditis elegans CELE_F20D6.11   Protein F20D6.11
Drosophila melanogaster Dmel_CG10700   CG10700 gene product from transcript CG10700-RA
Drosophila melanogaster Dmel_CG4199   CG4199 gene product from transcript CG4199-RG
Escherichia coli b2711   NADH:flavorubredoxin oxidoreductase
Escherichia coli b3365   nitrite reductase, large subunit, NAD(P)H-binding
Escherichia coli b2542   phenylpropionate dioxygenase, ferredoxin reductase subunit
Entamoeba histolytica EHI_153000   pyridine nucleotide-disulfide oxidoreductase family protein
Giardia lamblia GL50803_33769   NADH oxidase lateral transfer candidate
Homo sapiens ENSG00000183773   apoptosis-inducing factor, mitochondrion-associated, 3
Mus musculus ENSMUSG00000022763   apoptosis-inducing factor, mitochondrion-associated 3
Mycobacterium tuberculosis Rv0688   Putative ferredoxin reductase
Mycobacterium tuberculosis Rv3303c   NAD(P)H quinone reductase LpdA
Mycobacterium tuberculosis Rv0252   Probable nitrite reductase [NAD(P)H] large subunit [FAD flavoprotein] NirB
Mycobacterium tuberculosis Rv1869c   Probable reductase
Mycobacterium ulcerans MUL_0769   ferredoxin reductase
Mycobacterium ulcerans MUL_2668   flavoprotein disulfide reductase
Neospora caninum NCLIV_029160   Pyridine nucleotide-disulphide oxidoreductase family protein, related
Neospora caninum NCLIV_002590   Phthalate dioxygenase reductase subunit, related
Oryza sativa 4346299   Os08g0557600
Oryza sativa 4330469   Os02g0707100
Oryza sativa 4330468   Os02g0707000
Oryza sativa 4344670   Os08g0151800
Oryza sativa 4347885   Os09g0567300
Plasmodium berghei PBANKA_0617900   ferrodoxin reductase-like protein, putative
Plasmodium falciparum PF3D7_0720400   ferrodoxin reductase-like protein
Plasmodium knowlesi PKNH_0315800   ferrodoxin reductase-like protein
Plasmodium vivax PVX_096305   ferrodoxin reductase, putative
Plasmodium yoelii PY01431   Tamegoloh, putative
Toxoplasma gondii TGME49_207620   pyridine nucleotide-disulfide oxidoreductase domain-containing protein
Toxoplasma gondii TGME49_255890   pyridine nucleotide-disulfide oxidoreductase domain-containing protein
Treponema pallidum TP0921   NADH oxidase
Theileria parva TP03_0672   oxidoreductase, putative

Essentiality

TGME49_255890 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu255 Mycobacterium tuberculosis non-essential nmpdr
mtu700 Mycobacterium tuberculosis non-essential nmpdr
mtu1899 Mycobacterium tuberculosis non-essential nmpdr
b2542 Escherichia coli non-essential goodall
b2711 Escherichia coli non-essential goodall
b3365 Escherichia coli non-essential goodall
TGME49_255890 this record Toxoplasma gondii Essentiality uncertain sidik
TGME49_207620 Toxoplasma gondii Essentiality uncertain sidik
TGME49_255890 this record Toxoplasma gondii Probably non-essential sidik
TGME49_207620 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_255890 (Toxoplasma gondii), pyridine nucleotide-disulfide oxidoreductase domain-containing protein
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