Detailed view for TGME49_261940

Basic information

TDR Targets ID: 260404
Toxoplasma gondii, hydrolase, alpha/beta fold family protein
Source Database / ID:  ToxoDB 

pI: 6.6243 | Length (AA): 844 | MW (Da): 92824 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF12146   Serine aminopeptidase, S33

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
31 282 4i4c (A) 64 298 17.00 0 0.53 0.374878 0.1
154 280 5a62 (A) 3 123 26.00 0 1 0.559374 -1
158 269 1a8q (A) 4 107 27.00 0.000000000024 1 0.437601 -0.36
164 412 4f0j (A) 54 295 21.00 0 1 0.548324 -0.06
166 585 5hz2 (A) 229 576 11.00 0 0.72 0.26493 1.28
171 218 4x00 (A) 32 78 45.00 0.16 0.57 0.365372 1.32
467 526 2wue (A) 222 275 43.00 0.017 0.55 0.39259 0.47
629 766 4uos (A) 17 156 14.00 0 0.01 0.392807 -1.19

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_140207)

Species Accession Gene Product
Babesia bovis BBOV_II006840   alpha/beta hydrolase protein, putative
Cryptosporidium hominis Chro.80075   hydrolase, alpha/beta fold family
Cryptosporidium parvum cgd8_600   secrted alpha/beta hydrolase superfamiy protein
Neospora caninum NCLIV_025680   Secrted alpha/beta hydrolase superfamiy protein, related
Plasmodium berghei PBANKA_0704400   alpha/beta hydrolase, putative
Plasmodium falciparum PF3D7_1401300   epoxide hydrolase 2
Plasmodium falciparum PF3D7_0826200   alpha/beta hydrolase, putative
Plasmodium knowlesi PKNH_1320400   alpha/beta hydrolase, putative
Plasmodium vivax PVX_089050   alpha/beta hydrolase, putative
Plasmodium yoelii PY05572   hydrolase, alpha/beta fold family, putative
Toxoplasma gondii TGME49_261940   hydrolase, alpha/beta fold family protein
Theileria parva TP02_0652   hypothetical protein

Essentiality

TGME49_261940 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_0704400 Plasmodium berghei Dispensable plasmo
TGME49_261940 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_261940 (Toxoplasma gondii), hydrolase, alpha/beta fold family protein
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