Detailed view for TGME49_312570

Basic information

TDR Targets ID: 261065
Toxoplasma gondii, CMGC kinase, MAPK family (ERK) MAPK-1

Source Database / ID:  ToxoDB 

pI: 6.9402 | Length (AA): 1298 | MW (Da): 136204 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004707   MAP kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 13 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
56 261 1rjb (A) 594 836 38.00 0.0000000082 1 0.29 0.21
96 547 2gph (A) 10 354 32.00 0 1 0.29 0.27
154 257 2ac3 (A) 125 229 34.00 0.0000052 1 0.55 -1.42
46 548 5ci6 (B) 37 392 44.00 0 1 0.309219 1.07
51 550 2baj (A) 0 353 38.00 0 1 0.227908 1.16
71 546 5byz (A) 49 389 48.00 0 1 0.469318 0.46
98 551 4e7w (A) 26 367 27.00 0 1 0.210469 1.08
110 307 4js8 (A) 525 736 21.00 0 0.77 0.425242 -0.47
133 543 2b9h (A) 35 347 47.00 0 1 0.423241 0.52
327 546 2xs0 (A) 141 364 24.00 0 1 0.374592 0.47
357 505 4ylk (A) 322 480 33.00 0.00000000073 0.99 0.438392 -0.56
359 505 3llt (A) 737 875 28.00 0.00051 0.98 0.429851 -0.6
470 545 4qny (A) 276 350 45.00 0 0.99 0.273652 1.13

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 merozoite, ME49 Oocyst. Hehl AB Fritz HM
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_126781)

Species Accession Gene Product
Arabidopsis thaliana AT1G01560   mitogen-activated protein kinase 11
Arabidopsis thaliana AT2G18170   mitogen-activated protein kinase 7
Arabidopsis thaliana AT3G45640   mitogen-activated protein kinase 3
Arabidopsis thaliana AT4G01370   mitogen-activated protein kinase 4
Arabidopsis thaliana AT3G59790   mitogen-activated protein kinase 10
Arabidopsis thaliana AT4G11330   mitogen-activated protein kinase 5
Arabidopsis thaliana AT4G36450   mitogen-activated protein kinase 14
Arabidopsis thaliana AT1G10210   mitogen-activated protein kinase 1
Arabidopsis thaliana AT1G59580   mitogen-activated protein kinase 2
Arabidopsis thaliana AT1G07880   mitogen-activated protein kinase 13
Arabidopsis thaliana AT2G43790   MAP kinase 6
Brugia malayi Bm1_46485   MAP kinase sur-1
Candida albicans CaO19.10404   MAP kinase-like orf
Candida albicans CaO19.2886   MAP kinase-like orf
Candida albicans CaO19.8091   Serine/threonine protein kinase of MAP kinase family
Candida albicans CaO19.7523   MAP kinase implicated in PKC1-controlled signalling pathway
Candida albicans CaO19.460   Serine/threonine protein kinase of MAP kinase family
Caenorhabditis elegans CELE_F43C1.2   Protein MPK-1, isoform A
Cryptosporidium hominis Chro.30343   MAPK
Cryptosporidium parvum cgd3_3030   MAPK, putative
Dictyostelium discoideum DDB_G0286353   ERK subfamily protein kinase
Drosophila melanogaster Dmel_CG12559   rolled
Echinococcus granulosus EgrG_000803700   mitogen activated protein kinase 3
Echinococcus granulosus EgrG_000891000   mitogen activated protein kinase
Echinococcus multilocularis EmuJ_000891000   mitogen activated protein kinase
Echinococcus multilocularis EmuJ_000803700   mitogen activated protein kinase 3
Giardia lamblia GL50803_17563   Kinase, CMGC MAPK
Homo sapiens ENSG00000102882   mitogen-activated protein kinase 3
Homo sapiens ENSG00000166484   mitogen-activated protein kinase 7
Homo sapiens ENSG00000100030   mitogen-activated protein kinase 1
Leishmania braziliensis LbrM.33.1650   mitogen activated protein kinase, putative,map kinase, putative
Leishmania braziliensis LbrM.19.1710   mitogen activated protein kinase 4, putative;with=GeneDB:LmxM19.1440,map kinase
Leishmania donovani LdBPK_331470.1   mitogen activated protein kinase, putative
Leishmania donovani LdBPK_191480.1   mitogen-activated protein kinase 4
Leishmania infantum LinJ.19.1480   mitogen activated protein kinase 4, putative;with=GeneDB:LmxM19.1440
Leishmania infantum LinJ.33.1470   mitogen activated protein kinase, putative,map kinase, putative
Leishmania major LmjF.19.1440   mitogen activated protein kinase 4, putative;with=GeneDB:LmxM19.1440
Leishmania major LmjF.33.1380   mitogen activated protein kinase, putative,map kinase, putative
Leishmania mexicana LmxM.19.1440   mitogen-activated protein kinase
Leishmania mexicana LmxM.32.1380   mitogen activated protein kinase, putative,map kinase, putative
Loa Loa (eye worm) LOAG_08585   CMGC/MAPK/ERK1 protein kinase
Mus musculus ENSMUSG00000063358   mitogen-activated protein kinase 1
Mus musculus ENSMUSG00000063065   mitogen-activated protein kinase 3
Mus musculus ENSMUSG00000001034   mitogen-activated protein kinase 7
Oryza sativa 4340170   Os06g0154500
Oryza sativa 4344698   Os08g0157000
Oryza sativa 4349225   Os10g0533600
Oryza sativa 4332475   Os03g0285800
Oryza sativa 4341956   Os06g0699400
Oryza sativa 4328297   Os02g0148100
Saccharomyces cerevisiae YGR040W   mitogen-activated serine/threonine-protein kinase KSS1
Saccharomyces cerevisiae YBL016W   mitogen-activated serine/threonine-protein kinase FUS3
Saccharomyces cerevisiae YHR030C   mitogen-activated serine/threonine-protein kinase SLT2
Schistosoma japonicum Sjp_0132780   Mitogen-activated protein kinase 3, putative
Schistosoma japonicum Sjp_0044730   ko:K04371 extracellular signal-regulated kinase 1/2, putative
Schistosoma japonicum Sjp_0035520   Mitogen-activated protein kinase 3, putative
Schistosoma mansoni Smp_142050   serine/threonine protein kinase
Schmidtea mediterranea mk4.000508.08   Mitogen-activated protein kinase ERK-A
Schmidtea mediterranea mk4.002179.01  
Schmidtea mediterranea mk4.005156.02   Mitogen-activated protein kinase ERK-A
Schmidtea mediterranea mk4.017998.00   Mitogen-activated protein kinase mpk-1
Schmidtea mediterranea mk4.016766.00   Mitogen-activated protein kinase ERK-A
Schmidtea mediterranea mk4.001937.03   Mitogen-activated protein kinase ERK-A
Schmidtea mediterranea mk4.009672.00   Mitogen-activated protein kinase ERK-A
Schmidtea mediterranea mk4.000030.09  
Schmidtea mediterranea mk4.015572.01  
Trypanosoma brucei gambiense Tbg972.10.19640   mitogen-activated protein kinase, putative
Trypanosoma brucei gambiense Tbg972.10.14510   protein kinase, putative,tyrosine protein kinase, putative
Trypanosoma brucei Tb927.10.16030   mitogen activated protein kinase 4, putative
Trypanosoma brucei Tb11.v5.1057   protein kinase, putative
Trypanosoma congolense TcIL3000_10_10240   tyrosine protein kinase, putative
Trypanosoma cruzi TcCLB.511299.70   mitogen activated protein kinase 2, putative
Trypanosoma cruzi TcCLB.511837.70   mitogen-activated protein kinase 11, putative
Trypanosoma cruzi TcCLB.506483.69   mitogen activated protein kinase 4, putative
Trypanosoma cruzi TcCLB.510123.20   mitogen-activated protein kinase 11, putative
Toxoplasma gondii TGME49_312570 this record   CMGC kinase, MAPK family (ERK) MAPK-1
Trichomonas vaginalis TVAG_008800   CMGC family protein kinase
Trichomonas vaginalis TVAG_307130   CMGC family protein kinase
Trichomonas vaginalis TVAG_258130   CMGC family protein kinase
Trichomonas vaginalis TVAG_338030   CMGC family protein kinase

Essentiality

TGME49_312570 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.12040 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.12040 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.12040 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.12040 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.16030 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.16030 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.16030 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.16030 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F43C1.2 Caenorhabditis elegans embryonic lethal wormbase
CELE_F43C1.2 Caenorhabditis elegans larval arrest wormbase
CELE_F43C1.2 Caenorhabditis elegans larval lethal wormbase
CELE_F43C1.2 Caenorhabditis elegans sterile wormbase
TGME49_312570 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?
In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.
 
In any case, if you have information about papers containing relevant validation data for this target, please log in using your TDR Targets username and password and send them to us using the corresponding form in this page (only visible to registered users) or contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.7


Known modulators for this target

Compound Source Reference
ChEMBL23 References
ChEMBL23 References

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Mus musculus mitogen-activated protein kinase 1 Compounds References
Homo sapiens mitogen-activated protein kinase 3 Compounds References
Mus musculus mitogen-activated protein kinase 3 Compounds References
Rattus norvegicus Mitogen-activated protein kinase 1 Compounds References
Homo sapiens mitogen-activated protein kinase 1 Compounds References
Homo sapiens mitogen-activated protein kinase 7 Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets

Obtained from network model

Ranking Plot


Putative Drugs List


Compound Raw Global Species
0.0891 0.5028 1
0.0439 0.5 0.5
0.3356 0.7832 0.7832
0.2775 0.5 0.5
0.0997 0.5 0.5
0.005 0.2511 0.1874
0.1512 0.5 0.5
0.113 0.5 0.5
0.2386 0.5 0.5
0.0062 0.5 0.5
0.0062 0.4044 0.5
0.0062 0.8256 0.9317
0.0158 0.3414 1
0.3727 0.5 0.5
0.1768 0.5 0.5
0.0062 0.5 0.5
0.1458 0.5 0.5
0.0062 0.2634 1
0.0202 0.2743 1
0.2077 0.5 0.5
0.1116 0.5 0.5
0.2751 0.5483 1
0.0209 0.2837 1
0.0528 0.5 0.5
0.0868 0.3495 1
0.0868 0.3495 1
0.0218 0.2833 1
0.0868 0.3495 1
0.04 0.5 0.5
0.0747 0.5 0.5
0.343 0.5 0.5
0.494 0.5 0.5
0.026 0.2722 1
0.4596 0.5 0.5
0.061 0.5 0.5
0.0915 0.5 0.5
0.1702 0.5 0.5
0.0062 0.3473 0.4076
0.0063 0.5439 0.8468
0.1725 0.5 0.5
0.3403 0.5 0.5
0.1547 0.5 0.5
0.2342 0.5 0.5
0.0062 0.3522 1
0.0199 0.5 0.5
0.0467 0.5451 1
0.0062 0.4044 0
0.1888 0.5302 1
0.0112 0.2971 1
0.2077 0.5 0.5
0.0062 0.5 0.5
0.0231 0.5 0.5
0.482 0.5 0.5
0.0697 0.4527 0.5
0.0933 0.9521 1
0.523 0.5 0.5
0.1026 0.2574 0.8728
0.2495 0.5894 1
0.0063 0.5885 0.5885
0.2638 0.5 0.5
0.0206 0.2579 1
0.2826 0.5 0.5
0.085 0.4463 1
0.1333 0.2713 0.5
0.0557 0.5 0.5
0.0062 0.2639 0.5
0.3731 0.5339 0.5329
0.0062 0.2572 1
0.0671 0.5 0.5
0.0273 0.2689 0.365
0.066 0.3926 1
0.0617 0.6718 1
0.0716 0.5 0.5
0.0281 0.4742 0.5
0.0515 0.5 0.5
0.0398 0.5 0.5
0.0293 0.2639 0.3982
0.0537 0.6507 1
0.1189 0.5 0.5
0.0204 0.2547 1
0.0849 0.2731 1
0.0201 0.5576 0.5
0.0935 0.5 0.5
0.1883 0.3878 1
0.0421 0.4494 1
0.1973 0.5 0.5
0.1095 0.5 0.5
0.1068 0.3344 0.9155
0.0121 0.3347 1
0.0217 0.5 0.5
0.1189 0.5 0.5
0.1931 0.5 0.5
0.2166 0.5 0.5
0.0413 0.4004 0.5
0.038 0.3923 1
0.0062 0.2564 0.5
0.0062 0.5267 0.5
0.1883 0.3878 1
0.0062 0.3303 1
0.164 0.5 0.5
0.0058 0.2562 1
0.2751 0.5483 1
0.1068 0.3344 0.9155
0.0433 0.3765 1
0.0112 0.6218 1
0.1482 0.9726 1
0.0637 0.325 0
0.0212 0.9296 1
0.0102 0.295 0.3772
0.2141 0.5035 0.7532
0.0553 0.6779 1
0.0061 0.396 1
0.1418 0.5 0.5
0.0058 0.8178 1
0.0049 0.4728 0.5
0.0062 0.9537 0.5
0.0062 0.5 0.5
0.0106 0.274 1
0.4106 0.5 0.5
0.0467 0.5451 1
0.1013 0.2854 1
0.0617 0.6718 1
0.1296 0.5 0.5
0.0617 0.6718 1
0.0063 0.7244 0.7244
0.1677 0.5 0.5
0.0062 0.4492 1
0.0502 0.5228 1
0.3708 0.5 0.5
0.005 0.4325 0.5894
0.0062 0.8131 0.5894
0.2311 0.3768 0.8467
0.3708 0.5 0.5
0.0062 0.8957 0.5
0.258 0.5 0.5
0.0301 0.6029 1
0.01 0.4613 0
0.2759 0.5 0.5
0.0537 0.6507 1
0.2355 0.8681 1
0.1445 0.5721 1
0.02 0.2863 0.6373
0.0227 0.6122 1
0.0231 0.8955 1
0.0747 0.5 0.5
0.0062 0.2805 1
0.0062 0.4069 1
0.1136 0.2998 1
0.0929 0.5 0.5
0.0061 0.6883 0.6656
0.1662 0.5 0.5
0.3877 0.4244 0.5657
0.0211 0.5 0.5
0.0454 0.3024 0
0.0249 0.5 0.5
0.1883 0.3878 1
0.0053 0.8265 0.9317
0.2141 0.5035 0.7532
0.1097 0.4411 1
0.0633 0.5 0.5
0.0063 0.9 0.6245
0.2565 0.5 0.5
0.066 0.3926 1
0.0362 0.4268 1
0.2141 0.5035 0.7532
0.0108 0.743 1
0.5156 0.4858 1
0.519 0.5 0.5
0.0207 0.2554 1
0.0062 0.2522 0.7779
0.3356 0.7832 0.7832
0.0062 0.6754 1
0.1439 0.4286 1
0.2882 0.5 0.5
0.0062 0.3328 1
0.1284 0.5 0.5
0.1142 0.5 0.5
0.3541 0.5 0.5
0.0109 0.3849 1
0.0062 0.3194 0.5
0.2712 0.5 0.5
0.0433 0.3765 1
0.0109 0.3849 1
0.2173 0.3193 1
0.0467 0.5451 1
0.0868 0.3495 1
0.2759 0.5 0.5
0.1333 0.2713 0.5
0.0062 0.3796 1
0.0868 0.3495 1
0.1735 0.8321 0
0.1333 0.2713 0.5
0.1883 0.3878 1
0.2296 0.5 0.5
0.0994 0.3871 1
0.0053 0.9621 1

Assayability

Assay information

  • Assay for Protein Kinase C (2.7.1.37 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • ChEMBL
  • Inhibition of Toxoplasma gondii recombinant MAPK1 expressed in Escherichia coli

Reagent availability

No reagent availability information for this target.

Bibliographic References

52 literature references were collected for this gene.

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Gene identifier TGME49_312570 (Toxoplasma gondii), CMGC kinase, MAPK family (ERK) MAPK-1
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