Detailed view for TGME49_271460

Basic information

TDR Targets ID: 261597
Toxoplasma gondii, protein c14orf29, putative
Source Database / ID:  ToxoDB 

pI: 7.5948 | Length (AA): 884 | MW (Da): 97596 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF12146   Serine aminopeptidase, S33

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
43 323 1tht (A) 4 292 15.00 0 1 0.61 -0.83
58 294 1mtz (A) 11 293 18.00 0 1 0.56 -1.25
1 180 3k9b (A) 1060 1250 17.00 0.63 0.57 0.24682 0.4
4 296 2ecf (A) 432 741 18.00 0 0.95 0.304648 0.88
31 305 3azo (A) 374 656 16.00 0 1 0.444286 0.13
51 175 2e3j (A) 7 125 26.00 0.00000000045 0.98 0.509603 -0.74
55 179 2i3d (A) 9 133 19.00 0 0.93 0.411803 -1.22
82 186 3trd (A) 39 147 33.00 0.91 0.7 0.421978 0.19
474 593 2y44 (A) 75 224 32.00 0.35 0.17 0.264947 -0.3
615 788 5cwq (A) 40 207 16.00 0 0.08 0.412233 -1.18

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Show/Hide expression data references
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_130566)

Species Accession Gene Product
Arabidopsis thaliana AT4G14290   alpha/beta-hydrolase family protein
Arabidopsis thaliana AT4G17150   alpha/beta-Hydrolases superfamily protein
Arabidopsis thaliana AT3G23540   alpha/beta-Hydrolases superfamily protein
Cryptosporidium hominis Chro.70050   random slug cDNA-11 (Fragment)
Cryptosporidium parvum cgd7_370   protein with a conserved N-terminal region
Dictyostelium discoideum DDB_G0272791   alpha/beta hydrolase fold-1 domain-containing protein
Dictyostelium discoideum DDB_G0295699   alpha/beta hydrolase fold-1 domain-containing protein
Leishmania braziliensis LbrM.30.0470   hypothetical protein, conserved
Leishmania donovani LdBPK_300400.1   Alpha/beta hydrolase family, putative
Leishmania infantum LinJ.30.0400   hypothetical protein, conserved
Leishmania major LmjF.30.0400   hypothetical protein, conserved
Leishmania mexicana LmxM.29.0400   hypothetical protein, conserved
Neospora caninum NCLIV_035460   Alpha/beta hydrolase, related
Oryza sativa 9266683   Os02g0197900
Oryza sativa 4341501   Os06g0609700
Plasmodium berghei PBANKA_0707200   alpha/beta hydrolase, putative
Plasmodium falciparum PF3D7_0823400   alpha/beta hydrolase, putative
Plasmodium knowlesi PKNH_1317400   alpha/beta hydrolase, putative
Plasmodium vivax PVX_089195   alpha/beta hydrolase, putative
Plasmodium yoelii PY02678   hypothetical protein
Trypanosoma brucei gambiense Tbg972.6.1510   hypothetical protein, conserved
Trypanosoma brucei Tb927.6.1810   Alpha/beta hydrolase family, putative
Trypanosoma congolense TcIL3000_6_1400   Alpha/beta hydrolase family, putative
Trypanosoma cruzi TcCLB.511491.120   Alpha/beta hydrolase family, putative
Toxoplasma gondii TGME49_271460   protein c14orf29, putative
Trichomonas vaginalis TVAG_292600   conserved hypothetical protein
Trichomonas vaginalis TVAG_316560   Clan SC, family S9, unassigned serine peptidase
Trichomonas vaginalis TVAG_479170   Clan SC, family S9, unassigned serine peptidase
Trichomonas vaginalis TVAG_329180   conserved hypothetical protein
Trichomonas vaginalis TVAG_112980   conserved hypothetical protein
Trichomonas vaginalis TVAG_043720   Clan SC, family S9, unassigned serine peptidase
Trichomonas vaginalis TVAG_233340   conserved hypothetical protein
Trichomonas vaginalis TVAG_112930   conserved hypothetical protein
Trichomonas vaginalis TVAG_011790   Clan SC, family S9, unassigned serine peptidase
Trichomonas vaginalis TVAG_460750   conserved hypothetical protein
Trichomonas vaginalis TVAG_173950   conserved hypothetical protein
Trichomonas vaginalis TVAG_374960   Clan SC, family S33, methylesterase-like serine peptidase
Trichomonas vaginalis TVAG_262420   Clan SC, family S9, unassigned serine peptidase
Trichomonas vaginalis TVAG_369930   conserved hypothetical protein

Essentiality

TGME49_271460 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.1810 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.1810 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.6.1810 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.1810 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_271460 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TGME49_271460 (Toxoplasma gondii), protein c14orf29, putative
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