Detailed view for TGME49_231130

Basic information

TDR Targets ID: 261903
Toxoplasma gondii, hypothetical protein
Source Database / ID:  ToxoDB 

pI: 8.245 | Length (AA): 711 | MW (Da): 77125 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 3

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04389   Peptidase family M28

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
129 452 1rtq (A) 4 291 15.00 0 1 0.53 -0.82
258 550 1tkj (A) 6 273 19.00 0.000021 1 0.426496 -0.17
308 549 3pb6 (X) 138 380 28.00 0.086 1 0.474666 0.58
508 615 3giw (A) 122 238 32.00 0.04 0.14 0.283199 0.95

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite. Gregory Hehl AB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Oocyst, ME49 Bradyzoite. Fritz HM Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_132252)

Species Accession Gene Product
Arabidopsis thaliana AT3G44330   hypothetical protein
Brugia malayi Bm1_29425   nicalin
Caenorhabditis elegans CELE_T05F1.1   Protein NRA-2, isoform B
Dictyostelium discoideum DDB_G0271012   hypothetical protein
Drosophila melanogaster Dmel_CG4972   CG4972 gene product from transcript CG4972-RA
Echinococcus granulosus EgrG_000825400   nicalin
Echinococcus multilocularis EmuJ_000825400   nicalin
Homo sapiens ENSG00000125912   nicalin
Loa Loa (eye worm) LOAG_04256   hypothetical protein
Loa Loa (eye worm) LOAG_11503   hypothetical protein
Mus musculus ENSMUSG00000020238   nicalin homolog (zebrafish)
Neospora caninum NCLIV_031500   hypothetical protein
Oryza sativa 4344424   Os08g0102100
Schistosoma japonicum Sjp_0008170   Nicalin-1 precursor, putative
Schistosoma mansoni Smp_170400   nicalin (M28 family)
Schmidtea mediterranea mk4.000996.03  
Toxoplasma gondii TGME49_231130   hypothetical protein
Trichomonas vaginalis TVAG_318930   conserved hypothetical protein

Essentiality

TGME49_231130 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_T05F1.1 Caenorhabditis elegans sterile wormbase
TGME49_231130 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0582 0.5175 0.5
0.0205 0.4749 0.5
0.0233 0.356 0.5
0.0318 0.3394 0.5
0.0249 0.436 1
0.0275 0.4652 1
0.0233 0.356 0.5
0.0429 0.4597 1
0.0318 0.3394 0.5
0.0385 0.3517 1
0.045 0.2824 0.5
0.0284 0.337 1
0.045 0.4215 0.5
0.0385 0.3517 1
0.045 0.4215 0.5
0.0172 0.2618 1
0.0267 0.5372 1
0.0205 0.4749 0.5
0.0582 0.5175 0.5
0.0182 0.3513 0.5
0.0225 0.644 1
0.0228 1 0.5
0.0207 0.6634 0.5
0.0148 0.5 0.5
0.0153 0.661 0.8117
0.0608 0.4599 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_231130 (Toxoplasma gondii), hypothetical protein
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