Detailed view for TGME49_305260

Basic information

TDR Targets ID: 262235
Toxoplasma gondii, hypothetical protein
Source Database / ID:  ToxoDB 

pI: 6.5064 | Length (AA): 594 | MW (Da): 64079 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF12146   Serine aminopeptidase, S33

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
128 510 1lzl (A) 2 318 17.00 0 0.97 0.37 0.17
243 507 1xkl (A) 3 259 13.00 0 0.24 0.26 -0.83
5 144 4ke2 (A) 8 128 36.00 0.0092 0.18 0.35439 0.81
229 288 1imj (A) 14 81 18.00 0.06 0.08 0.0524101 0.96
248 504 3llc (A) 48 265 22.00 0 0.93 0.44326 0.74

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite. Gregory
Show/Hide expression data references
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_144587)

Species Accession Gene Product
Entamoeba histolytica EHI_086370   hydrolase, alpha/beta fold family domain containing protein
Neospora caninum NCLIV_001135   hypothetical protein
Plasmodium falciparum PF3D7_1401500   esterase, putative
Plasmodium falciparum PF3D7_0936700   lysophospholipase, putative
Plasmodium falciparum PF3D7_1252600   esterase, putative
Plasmodium vivax PVX_097560   lysophospholipase, putative
Plasmodium vivax PVX_203290   unspecified product
Toxoplasma gondii TGME49_305260   hypothetical protein
Theileria parva TP03_0739   hypothetical protein
Theileria parva TP03_0200   hypothetical protein
Theileria parva TP04_0172   hypothetical protein
Theileria parva TP03_0738   hypothetical protein
Trichomonas vaginalis TVAG_364450   conserved hypothetical protein

Essentiality

TGME49_305260 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_305260 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Plasmodium falciparum esterase, putative Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Plasmodium falciparum lysophospholipase, putative 353 aa 24.3% 313 aa Compounds References
Plasmodium falciparum lysophospholipase, putative 371 aa 23.1% 308 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0478 0.5187 0.7471
0.0142 0.9044 0.8578
0.0308 0.3448 0.6796
0.0312 0.3458 0.6796
0.0293 1 1
0.0355 0.533 1
0.0644 0.3094 0.8044
0.031 0.3555 0.6813
0.0144 0.2648 0.4602
0.0301 0.331 0.672
0.0145 0.2804 1
0.051 0.7305 1
0.0178 0.3139 1
0.0678 0.6077 0.6697
0.0297 0.3466 0.3556
0.03 0.3474 0.6795
0.0326 0.3443 0.6125
0.0644 0.309 0.8044
0.0299 0.2979 0.67
0.0178 1 0.5
0.0146 0.2867 1
0.0146 0.3287 0.9204
0.0069 0.4643 0.9034
0.0147 0.2842 0.469
0.0334 0.3387 0.5977
0.0308 0.3458 0.6796
0.0299 0.2979 0.67
0.0143 0.4229 1
0.0449 0.4729 0.7472
0.0305 0.346 0.6697
0.0483 0.4806 1
0.0644 0.309 0.8044
0.0477 0.4902 0.7373
0.0145 0.2804 1
0.0173 1 0.5
0.0308 0.3459 0.6796
0.0533 0.7305 1
0.0644 0.309 0.8044
0.0793 0.6586 0.7908
0.051 0.7305 1
0.0743 0.7271 0.806
0.0145 0.2779 1
0.0178 0.9186 0.9154
0.0308 0.3453 0.6722
0.0178 0.9044 0.8578
0.0291 0.3557 0.6814
0.0293 0.3562 0.8011
0.0146 0.2648 0.4602
0.0301 0.3455 0.6698
0.0145 0.2779 1
0.0296 1 0.5
0.0355 0.4784 1
0.0144 0.2782 1
0.0297 0.3466 0.3556
0.0146 0.2906 0.4436
0.0144 0.2804 1
0.0327 0.336 0.6878
0.0509 0.7285 1
0.0297 0.3466 0.3556
0.0308 0.3459 0.6796
0.076 0.3329 0.332
0.0509 0.7305 1
0.0533 0.7313 1
0.0293 1 1
0.0326 0.3443 0.6125
0.0188149 1 1
0.0589 0.4367 0.4674
0.0297 0.3466 0.3556
0.0145 1 0.5
0.0644 0.309 0.8044
0.031 0.3555 0.6813
0.0334 0.3462 0.6818
0.0308 0.3469 0.677
0.0457 0.4802 1
0.0509 0.7285 1
0.0326 0.3443 0.6125
0.0177 0.9044 0.8578
0.0326 0.3443 0.6125
0.0323 0.3345 0.5901
0.0145 1 0.5
0.0144 0.3377 0.9206
0.0292 0.3473 0.6771
0.0449 0.4729 0.7472
0.0861 0.727 0.806
0.0483 0.4816 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_305260 (Toxoplasma gondii), hypothetical protein
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