Detailed view for LmjF.01.0180

Basic information

TDR Targets ID: 26274
Leishmania major, CLC-type chloride channel, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.4523 | Length (AA): 772 | MW (Da): 84037 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 10

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00654   Voltage gated chloride channel

Gene Ontology

Mouse over links to read term descriptions.
GO:0016020   membrane  
GO:0005247   voltage-gated chloride channel activity  
GO:0005216   ion channel activity  
GO:0055085   transmembrane transport  
GO:0006821   chloride transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 11 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
89 557 1ots (A) 37 445 24.00 0 1 0.85 -1.37
570 729 2d4z (B) 528 770 16.00 0 0.81 0.41 -0.86
578 729 2d4z (B) 536 770 14.00 0.00005 0.94 0.4 -0.68
88 558 1kpl (A) 36 446 24.00 0 1 0.703704 0.72
88 558 4ene (A) 36 446 24.00 0 0.61 0.662704 0.74
154 557 4ene (A) 70 445 26.00 0 1 0.678516 0.58
177 562 3nd0 (A) 91 442 28.00 0 0.35 0.6052 0.83
591 742 2j9l (A) 596 750 22.00 0 1 0.545491 -0.6
678 755 2yvx (A) 202 280 9.00 0.015 0.09 0.116836 0.52
683 727 1y5h (A) 79 123 13.00 0.027 0.49 0.19709 0.19
684 771 3kh5 (A) 160 263 17.00 0.0051 0.38 0.15079 -0.3

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_126837)

Species Accession Gene Product
Arabidopsis thaliana AT1G55620   chloride channel protein CLC-f
Arabidopsis thaliana AT4G35440   chloride channel protein CLC-e
Brugia malayi Bm1_19435   CLC chloride channel protein
Candida albicans CaO19.3734   one of three likely voltage-gated chloride channel genes
Candida albicans CaO19.11219   one of three likely voltage-gated chloride channel genes
Caenorhabditis elegans CELE_C07H4.2   Protein CLH-5
Drosophila melanogaster Dmel_CG5284   Chloride channel-c
Escherichia coli b1592   H(+)/Cl(-) exchange transporter
Escherichia coli b0155   H(+)/Cl(-) exchange transporter
Echinococcus granulosus EgrG_000209700   H:Cl exchange transporter 4
Echinococcus granulosus EgrG_000843900   H:Cl exchange transporter 3
Echinococcus multilocularis EmuJ_000843900   H(+):Cl() exchange transporter 3
Echinococcus multilocularis EmuJ_000209700   H(+):Cl() exchange transporter 4
Homo sapiens ENSG00000073464   chloride channel, voltage-sensitive 4
Homo sapiens ENSG00000109572   chloride channel, voltage-sensitive 3
Homo sapiens ENSG00000171365   chloride channel, voltage-sensitive 5
Leishmania braziliensis LbrM.01.0210   CLC-type chloride channel, putative
Leishmania donovani LdBPK_010180.1   CLC-type chloride channel, putative
Leishmania infantum LinJ.01.0180   CLC-type chloride channel, putative
Leishmania major LmjF.01.0180   CLC-type chloride channel, putative
Leishmania mexicana LmxM.01.0180   CLC-type chloride channel, putative
Loa Loa (eye worm) LOAG_10527   chloride channel 3
Loa Loa (eye worm) LOAG_12515   hypothetical protein
Mus musculus ENSMUSG00000000605   chloride channel 4-2
Mus musculus ENSMUSG00000004317   chloride channel 5
Mus musculus ENSMUSG00000004319   chloride channel 3
Mycobacterium tuberculosis Rv0143c   Probable conserved transmembrane protein
Oryza sativa 9267257   Os01g0704700
Oryza sativa 4330553   Os02g0720700
Oryza sativa 4345939   Os08g0499200
Saccharomyces cerevisiae YJR040W   Gef1p
Schistosoma japonicum Sjp_0019790   Chloride channel protein 4, putative
Schistosoma japonicum Sjp_0019760   Chloride channel protein 4, putative
Schistosoma japonicum Sjp_0052730   ko:K05012 chloride channel 3, putative
Schistosoma mansoni Smp_071970   chloride channel protein
Schmidtea mediterranea mk4.001298.02   Chloride channel protein,putative
Schmidtea mediterranea mk4.052451.01  
Schmidtea mediterranea mk4.001298.03  
Trypanosoma cruzi TcCLB.504797.140   CLC-type chloride channel, putative
Trichomonas vaginalis TVAG_395090   chloride channel protein 3, 4, putative
Trichomonas vaginalis TVAG_032700   chloride channel, putative

Essentiality

LmjF.01.0180 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu144 Mycobacterium tuberculosis non-essential nmpdr
b0155 Escherichia coli non-essential goodall
b1592 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.01.0180 (Leishmania major), CLC-type chloride channel, putative
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