Detailed view for TGME49_240210

Basic information

TDR Targets ID: 263062
Toxoplasma gondii, phosphate transporter family protein, putative
Source Database / ID:  ToxoDB 

pI: 7.5452 | Length (AA): 869 | MW (Da): 92107 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 11

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01384   Phosphate transporter family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016020   membrane  
GO:0005315   inorganic phosphate transmembrane transporter activity  
GO:0006817   phosphate transport  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
20 290 1l7v (A) 14 281 10.00 0 0 0.42 -0.49
372 503 2mqa (A) 17 138 16.00 0 0.04 0.246999 -0.58
461 507 1jvr (A) 90 136 40.00 0.5 0.09 0.432785 -0.58

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite. Gregory
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_126728)

Species Accession Gene Product
Arabidopsis thaliana AT3G26570   1
Babesia bovis BBOV_II005570   phosphate transporter, putative
Brugia malayi Bm1_35205   Phosphate transporter family protein
Candida albicans CaO19.4599   probable Na+/Pi symporter
Candida albicans CaO19.12068   probable Na+/Pi symporter
Caenorhabditis elegans CELE_F09G2.3   Protein PITR-5
Caenorhabditis elegans CELE_C48A7.2   Protein PITR-1
Caenorhabditis elegans CELE_B0222.3   Protein PITR-3
Caenorhabditis elegans CELE_B0222.2   Protein PITR-2
Chlamydia trachomatis CT_692   phosphate permease
Drosophila melanogaster Dmel_CG42575   CG42575 gene product from transcript CG42575-RA
Escherichia coli b3493   phosphate transporter, low-affinity
Escherichia coli b2987   phosphate transporter
Echinococcus granulosus EgrG_000971100   sodium dependent phosphate transporter 1
Echinococcus granulosus EgrG_000971400   sodium dependent phosphate transporter 2
Echinococcus multilocularis EmuJ_000971400   sodium dependent phosphate transporter 2
Echinococcus multilocularis EmuJ_000971100   sodium dependent phosphate transporter 1
Homo sapiens ENSG00000168575   solute carrier family 20 (phosphate transporter), member 2
Homo sapiens ENSG00000144136   solute carrier family 20 (phosphate transporter), member 1
Leishmania braziliensis LbrM.28.3250   phosphate-repressible phosphate permease
Leishmania braziliensis LbrM.10.0030   phosphate-Repressible Phosphate Permease-like protein
Leishmania braziliensis LbrM.10.1450   phosphate-Repressible Phosphate Permease-like protein
Leishmania braziliensis LbrM.20.4120   phosphate-repressible phosphate permease
Leishmania donovani LdBPK_200040.1   phosphate-repressible phosphate permease
Leishmania donovani LdBPK_030480.1   phosphate-Repressible Phosphate Permease-like protein
Leishmania donovani LdBPK_101440.1   phosphate-Repressible Phosphate Permease-like protein
Leishmania infantum LinJ.20.0040   phosphate-repressible phosphate permease
Leishmania infantum LinJ.10.1440   phosphate-Repressible Phosphate Permease-like protein
Leishmania infantum LinJ.03.0480   phosphate-Repressible Phosphate Permease-like protein
Leishmania infantum LinJ.10.0010   phosphate-Repressible Phosphate Permease-like protein
Leishmania major LmjF.03.0500   phosphate-Repressible Phosphate Permease-like protein
Leishmania major LmjF.10.1300   phosphate-Repressible Phosphate Permease-like protein
Leishmania major LmjF.10.0030   phosphate-Repressible Phosphate Permease-like protein
Leishmania mexicana LmxM.03.0500   phosphate-Repressible Phosphate Permease-like protein
Leishmania mexicana LmxM.20.0031   phosphate-repressible phosphate permease
Leishmania mexicana LmxM.10.1300   phosphate-Repressible Phosphate Permease-like protein
Leishmania mexicana LmxM.10.0030   phosphate-Repressible Phosphate Permease-like protein
Loa Loa (eye worm) LOAG_11239   phosphate transporter
Loa Loa (eye worm) LOAG_08110   solute carrier family 20
Loa Loa (eye worm) LOAG_06338   phosphate transporter
Loa Loa (eye worm) LOAG_11120   phosphate permease
Loa Loa (eye worm) LOAG_11240   hypothetical protein
Loa Loa (eye worm) LOAG_11763   hypothetical protein
Mycobacterium leprae ML2260c   PROBABLE LOW-AFFINITY INORGANIC PHOSPHATE TRANSPORTER INTEGRAL MEMBRANE PROTEIN PITA
Mus musculus ENSMUSG00000027397   solute carrier family 20, member 1
Mus musculus ENSMUSG00000037656   solute carrier family 20, member 2
Mycobacterium tuberculosis Rv0545c   Probable low-affinity inorganic phosphate transporter integral membrane protein PitA
Mycobacterium tuberculosis Rv2281   Putative phosphate-transport permease PitB
Mycobacterium ulcerans MUL_2694   phosphate-transport permease PitB
Neospora caninum NCLIV_016480   hypothetical protein
Oryza sativa 4329844   Os02g0593500
Onchocerca volvulus OVOC7210  
Onchocerca volvulus OVOC7211  
Plasmodium berghei PBANKA_1354300   sodium-dependent phosphate transporter, putative
Plasmodium falciparum PF3D7_1340900   sodium-dependent phosphate transporter
Plasmodium knowlesi PKNH_1260500   sodium-dependent phosphate transporter, putative
Plasmodium vivax PVX_082960   sodium-dependent phosphate transporter, putative
Plasmodium yoelii PY01393   solute carrier family 20, member 1-related
Saccharomyces cerevisiae YBR296C   Pho89p
Schistosoma mansoni Smp_094660   phosphate transporter
Schmidtea mediterranea mk4.047682.01  
Schmidtea mediterranea mk4.043315.01  
Schmidtea mediterranea mk4.008985.00  
Trypanosoma brucei gambiense Tbg972.1.50   phosphate-repressible phosphate permease, putative
Trypanosoma brucei gambiense Tbg972.1.70   phosphate-repressible phosphate permease, putative
Trypanosoma brucei Tb927.1.600   phosphate-repressible phosphate permease, putative
Trypanosoma brucei Tb927.1.580   phosphate-repressible phosphate permease, putative
Trypanosoma congolense TcIL3000_0_52300   phosphate-repressible phosphate permease, putative
Trypanosoma congolense TcIL3000_1_150   phosphate-repressible phosphate permease, putative
Trypanosoma cruzi TcCLB.505999.10   phosphate-repressible phosphate permease, putative
Trypanosoma cruzi TcCLB.511417.90   phosphate-repressible phosphate permease, putative
Trypanosoma cruzi TcCLB.505999.20   phosphate-repressible phosphate permease, putative
Trypanosoma cruzi TcCLB.511417.80   phosphate-repressible phosphate permease, putative
Toxoplasma gondii TGME49_240210   phosphate transporter family protein, putative
Theileria parva TP02_0500   phosphate transporter, putative

Essentiality

TGME49_240210 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.1.600 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.1.600 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.1.600 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.1.600 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.1.580 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.1.580 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.1.580 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.1.580 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b2987 Escherichia coli non-essential goodall
b3493 Escherichia coli non-essential goodall
CELE_C48A7.2 Caenorhabditis elegans embryonic lethal wormbase
TGME49_240210 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_240210 (Toxoplasma gondii), phosphate transporter family protein, putative
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