Detailed view for TGME49_229780

Basic information

TDR Targets ID: 263133
Toxoplasma gondii, GHMP kinase, N-terminal domain-containing protein
Source Database / ID:  ToxoDB 

pI: 7.9672 | Length (AA): 923 | MW (Da): 99031 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00288   GHMP kinases N terminal domain
PF08544   GHMP kinases C terminal
PF10509   Galactokinase galactose-binding signature

Gene Ontology

Mouse over links to read term descriptions.
GO:0005534   galactose binding  
GO:0005737   cytoplasm  
GO:0016301   kinase activity  
GO:0005524   ATP binding  
GO:0016310   phosphorylation  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
322 396 5d2s (A) 99 173 15.00 0 0.01 0.517457 -3.17
348 804 2aj4 (A) 12 525 25.00 0 1 0.685125 0.42
447 782 2a2c (A) 112 440 37.00 0 1 0.72523 0.21
703 775 1kkh (A) 235 303 33.00 0.55 0.56 0.42299 -0.18

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 Bradyzoite. Gregory Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile ME49 Oocyst. Fritz HM
Show/Hide expression data references
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_129233)

Species Accession Gene Product
Arabidopsis thaliana AT3G06580   galactokinase
Brugia malayi Bm1_16455   galactokinase family protein
Candida albicans CaO19.11154   galactokinase similar to S. cerevisiae GAL3 (YDR009W)
Candida albicans CaO19.3670   galactokinase similar to S. cerevisiae GAL3 (YDR009W)
Caenorhabditis elegans CELE_M01D7.4   Protein TAG-96
Dictyostelium discoideum DDB_G0292112   hypothetical protein
Drosophila melanogaster Dmel_CG5288   Galactokinase
Echinococcus granulosus EgrG_000657000   N acetylgalactosamine kinase
Echinococcus multilocularis EmuJ_000657000   N acetylgalactosamine kinase
Homo sapiens 2585   galactokinase 2
Loa Loa (eye worm) LOAG_04522   galactokinase
Mus musculus ENSMUSG00000027207   galactokinase 2
Neospora caninum NCLIV_030570   hypothetical protein
Oryza sativa 4334679   Os03g0832600
Saccharomyces cerevisiae YBR020W   galactokinase
Saccharomyces cerevisiae YDR009W   transcriptional regulator GAL3
Schistosoma japonicum Sjp_0067540   ko:K00849 galactokinase [EC2.7.1.6], putative
Schistosoma japonicum Sjp_0067520   ko:K00849 galactokinase [EC2.7.1.6], putative
Schistosoma mansoni Smp_078400   galactokinase
Schmidtea mediterranea mk4.002893.00   N-acetylgalactosamine kinase
Schmidtea mediterranea mk4.013349.00   N-acetylgalactosamine kinase
Toxoplasma gondii TGME49_229780   GHMP kinase, N-terminal domain-containing protein
Trichomonas vaginalis TVAG_484130   galactokinase, putative
Trichomonas vaginalis TVAG_472360   galactokinase, putative
Trichomonas vaginalis TVAG_284490   galactokinase, putative
Trichomonas vaginalis TVAG_487340   galactokinase, putative
Trichomonas vaginalis TVAG_472220   galactokinase, putative
Trichomonas vaginalis TVAG_472380   galactokinase, putative

Essentiality

TGME49_229780 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_229780 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0298 0.4485 1
0.0252 0.447 1
0.0178 0.2636 0.5
0.0228 0.3511 1
0.026 0.4576 1
0.0353 0.4499 1
0.0144 0.3085 1
0.0167 0.3905 1
0.0327 0.339 1
0.0089 0.3273 0.5
0.03 0.2897 0.3586
0.0151 0.4932 1
0.0329 0.4445 1
0.011 0.4271 0.9731
0.036 0.3511 1
0.0259 0.3998 1
0.0276 0.4475 1
0.0152 0.493 1
0.025 0.4306 1
0.0329 0.313 1
0.0107 0.4186 1
0.0203 0.3667 1
0.0271 0.4433 1
0.0369 0.3782 0.739
0.0322 0.3643 1
0.0106 0.4277 1
0.0292 0.3511 1
0.0104 0.4203 1
0.0275 0.3195 0.3733
0.0159 0.4951 1
0.0151 0.4932 1
0.0277 0.4483 1
0.0352 0.4469 1
0.0343 0.4478 1
0.0343 0.4235 0.8396
0.0258 0.3859 1
0.0305 0.4063 1
0.0108 0.427 1
0.0327 0.339 1
0.0284 0.3936 1
0.0352 0.4435 1
0.0132 0.284 1
0.03 0.2897 0.3586
0.0304 0.4487 0.9916
0.0122 0.5004 1
0.0256 0.3093 0.7976
0.0183 0.3511 1
0.0328 0.3837 1
0.0112 0.3066 1
0.0184 0.4447 1
0.019 0.4329 1
0.0177 0.4023 1
0.018 0.3511 1
0.0298 0.4485 1
0.0252 0.4265 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_229780 (Toxoplasma gondii), GHMP kinase, N-terminal domain-containing protein
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