Detailed view for TGME49_225770

Basic information

TDR Targets ID: 263790
Toxoplasma gondii, Tyrosine kinase-like (TKL) protein
Source Database / ID:  ToxoDB 

pI: 6.1704 | Length (AA): 2015 | MW (Da): 214107 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
6 315 4wb7 (A) 38 335 25.00 0 1 0.268846 0.57
20 328 2j0k (A) 337 679 28.00 0.000000058 0.96 0.19505 0.86
39 330 4l68 (A) 279 580 27.00 0 1 0.449913 -0.43
54 327 3tl8 (A) 276 565 36.00 0 1 0.51998 -0.32
55 328 2nru (A) 168 457 32.00 0 1 0.47598 -0.48
60 339 2b9h (A) 6 326 23.00 0 0.93 0.183958 0.9
65 430 3mwu (A) 76 429 19.00 0 1 0.275638 0.68
510 918 4k6j (A) 640 1115 5.00 0 0 0.166978 -0.03
1293 1481 4ke2 (A) 3 191 14.00 0 0.07 0.185797 -0.04

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite, ME49 Oocyst. Gregory Fritz HM
Show/Hide expression data references
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_169473)

Species Accession Gene Product
Arabidopsis thaliana AT1G24030   protein kinase
Neospora caninum NCLIV_047060   Tyrosine kinase-like (TKL) protein, putative
Toxoplasma gondii TGME49_225770   Tyrosine kinase-like (TKL) protein

Essentiality

TGME49_225770 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_225770 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 27.6% 156 aa Compounds References
Rattus norvegicus Jak1 protein 210 aa 22.8% 206 aa Compounds References
Patiria pectinifera Cdc2 300 aa 28.4% 257 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 25.3% 245 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0033 1 1
0.0039 0.9485 0.5
0.0008 0.5 0.5
0.0022 0.5 0.5
0.0036 0.5 0.5
0.0059 1 1
0.0004 0.5 0.5
0.0081 0.5 0.5
0.0067 0.5 0.5
0.0042 0.5 0.5
0.0039 0.5 0.5
0.0081 1 0.5
0.0066 0.3101 0
0.0032 0.5 0.5
0.0012 0.5 0.5
0.0027 1 0.5
0.0069 0.3067 1
0.0026 0.5 0.5
0.0091 1 0.5
0.0003 0.5 0.5
0.0092 1 0.5
0.0007 0.5 0.5
0.0039 0.5 0.5
0.0063 0.7244 0.7244
0.0061 0.6883 0.6656
0.0093 0.8828 0
0.0029 0.5 0.5
0.0037 1 0.5
0.0018 0.5 0.5
0.0062 0.6935 0
0.0023 0.5 0.5
0.0011 1 0.5
0.0007 0.5 0.5
0.0033 0.5 0.5
0.0007 0.5 0.5
0.0012 0.5 0.5
0.0064 0.3377 0
0.0059 1 1
0.0016 0.5 0.5
0.0032 0.5 0.5
0.0059 1 1
0.0098 0.3242 0.2614
0.0012 0.5 0.5
0.0016 0.5 0.5
0.0056 1 0.5
0.0088 0.4477 1
0.0063 1 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

12 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_225770 (Toxoplasma gondii), Tyrosine kinase-like (TKL) protein
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