Detailed view for TGME49_307030

Basic information

TDR Targets ID: 264273
Toxoplasma gondii, Purine nucleoside phosphorylase
Source Database / ID:  ToxoDB 

pI: 7.4372 | Length (AA): 330 | MW (Da): 36054 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01048   Phosphorylase superfamily

Gene Ontology

Mouse over links to read term descriptions.
GO:0005737   cytoplasm  
GO:0004850   uridine phosphorylase activity  
GO:0003824   catalytic activity  
GO:0009166   nucleotide catabolic process  
GO:0009116   nucleoside metabolic process  

Metabolic Pathways

Pyrimidine metabolism (KEGG)
Global map (KEGG)

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
84 330 3mb8 (A) 4 250 99.99 0 1 2.00828 -1.65

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 3MB8:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 merozoite. Hehl AB
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Oocyst, ME49 Bradyzoite. Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile ME49 Tachyzoite. Gregory
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_130516)

Species Accession Gene Product
Babesia bovis BBOV_III005140   phosphorylase family protein
Dictyostelium discoideum DDB_G0284431   uridine phosphorylase
Drosophila melanogaster Dmel_CG17224   CG17224 gene product from transcript CG17224-RC
Escherichia coli b4384   purine-nucleoside phosphorylase
Entamoeba histolytica EHI_130960   purine nucleoside phosphorylase, putative
Entamoeba histolytica EHI_130930   purine nucleoside phosphorylase, putative
Neospora caninum NCLIV_053130   hypothetical protein
Plasmodium berghei PBANKA_1113000   purine nucleoside phosphorylase, putative
Plasmodium falciparum PF3D7_0513300   purine nucleoside phosphorylase
Plasmodium knowlesi PKNH_1019800   purine nucleoside phosphorylase, putative
Plasmodium vivax PVX_080575   purine nucleoside phosphorylase, putative
Plasmodium yoelii PY04622   purine nucleoside phosphorylase
Toxoplasma gondii TGME49_307030   Purine nucleoside phosphorylase
Treponema pallidum TP1027   uridine phosphorylase (udp)
Treponema pallidum TP0734   purine nucleoside phosphorylase (deoD)
Theileria parva TP02_0437   purine nucleoside phosphorylase, putative
Theileria parva TP02_0440   purine nucleoside phosphorylase, putative
Theileria parva TP02_0438   purine nucleoside phosphorylase, putative
Trichomonas vaginalis TVAG_127180   purine nucleoside phosphorylase, putative
Trichomonas vaginalis TVAG_454490   purine nucleoside phosphorylase, putative

Essentiality

TGME49_307030 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
b4384 Escherichia coli non-essential goodall
PBANKA_1113000 Plasmodium berghei Essential plasmo
TGME49_307030 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

Compound Source Reference
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
ChEMBL23 References
Curated by TDRTargets References
Curated by TDRTargets References

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Plasmodium falciparum purine nucleoside phosphorylase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.2536 0.8786 1
0.0197 0.5105 0.484
0.0921 0.8899 1
0.4428 0.4025 1
0.018 0.5 0.5
0.0319 1 1
0.0211 1 0.5
0.0211 1 0.5
0.0451 0.9877 0.5
0.4044 0.7351 1
0.3627 0.7741 1
0.4697 0.7926 1
0.3872 0.7864 1
0.3872 0.7864 1
0.0261 0.3959 1
0.0164 0.41 1
0.0211 1 0.5
0.2536 0.8786 1
0.3965 0.8137 1
0.1365 0.3996 1
0.0719 0.3598 0.5
0.4298 0.383 1
0.0144 0.2545 1
0.0147 0.32 1
0.0152 0.3736 1
0.0153 0.6136 1
0.3872 0.7864 1
0.4018 0.5238 1
0.0146 1 1
0.365 0.825 1
0.07 0.3377 0.5
0.4343 0.3029 1
0.0216 0.285 1
0.0243 1 0.5
0.0159 0.3007 1
0.3696 0.7306 1
0.0568 0.2609 1
0.4031 0.7833 1
0.4643 0.8049 1
0.0211 1 0.5
0.018 0.643 1
0.3199 0.7981 1
0.4298 0.383 1
0.016 0.3007 1
0.018 0.3007 1
0.0295 0.2611 1
0.365 0.825 1
0.4412 0.7191 0.5
0.018 0.3007 1
0.1741 0.8258 0.5
0.1372 0.8147 0.5
0.196 0.8458 0.5
0.0492 0.3293 1
0.018 0.7599 0.6467
0.2349 0.2613 0.5
0.4058 0.3709 0.5
0.0211 1 0.5
0.4592 0.802 1
0.0223 0.2518 0.2518
0.2982 0.8108 1
0.4644 0.7912 1
0.3872 0.7864 1
0.0152 0.3736 1
0.2564 0.2906 0.5
0.4058 0.3709 0.5
0.3983 0.7961 1
0.0151 1 1
0.4116 0.8263 1
0.0167 0.3902 1
0.0211 1 0.5
0.0261 0.3959 1
0.018 0.643 1
0.0243 1 0.5
0.1734 0.6451 1
0.1743 0.8336 1
0.0211 1 0.5
0.4455 0.7578 0.5
0.0568 0.2609 1
0.4298 0.383 1
0.0146 0.3007 1
0.018 0.3007 1
0.0197 0.5105 0.484
0.0211 1 0.5
0.4311 0.7679 1
0.0211 1 0.5
0.1734 0.6451 1
0.0211 1 0.5
0.0330744 0.481619 1
0.4298 0.383 1
0.3959 0.8038 1
0.0211 1 0.5
0.4695 0.8006 1
0.047 0.8385 1
0.018 0.3007 1
0.4428 0.4025 1
0.018 0.7599 0.6467
0.3113 0.8199 1
0.4133 0.8116 1
0.0407 0.2888 1
0.1734 0.6451 1
0.018 0.3007 1
0.018 0.3007 1
0.0211 1 0.5
0.0175 0.3304 1
0.2446 0.806 1
0.0211 1 0.5
0.0243 1 0.5
0.0991 0.8829 1
0.0789 0.6984 1
0.1365 0.3996 1
0.0172 0.7692 1
0.4133 0.8116 1
0.0451 0.9877 0.5

Assayability

Assay information

  • Inhibition of Toxoplasma gondii purine nucleoside phosphorylase ChEMBL
  • Inhibition of Toxoplasma gondii purine nucleoside phosphorylase

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_307030 (Toxoplasma gondii), Purine nucleoside phosphorylase
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