Detailed view for TGME49_226730

Basic information

TDR Targets ID: 264672
Toxoplasma gondii, aconitate hydratase ACN/IRP
Source Database / ID:  ToxoDB 

pI: 8.4816 | Length (AA): 1055 | MW (Da): 114885 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00330   Aconitase family (aconitate hydratase)
PF00694   Aconitase C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
17 733 1l5j (A) 151 829 18.00 0 1 0.553821 1.13
160 1049 2b3y (A) 3 889 58.00 0 1 1.6564 -1.51

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_126691)

Species Accession Gene Product
Arabidopsis thaliana AT4G35830   aconitate hydratase 1
Arabidopsis thaliana AT2G05710   aconitate hydratase 3
Arabidopsis thaliana AT4G26970   aconitase 2
Babesia bovis BBOV_III011790   aconitate hydratase 1 family protein
Brugia malayi Bm1_07420   aconitate hydratase, mitochondrial
Candida albicans CaO19.13953   likely mitochondrial aconitase family protein similar to S. cerevisiae YJL200C
Candida albicans CaO19.6632   likely mitochondrial aconitase family protein similar to S. cerevisiae YJL200C
Candida albicans CaO19.6385   similar to S. cerevisiae ACO1 (YLR304C) mitochondrial aconitate hydratase
Candida albicans CaO19.13742   similar to S. cerevisiae ACO1 (YLR304C) mitochondrial aconitate hydratase
Caenorhabditis elegans CELE_ZK455.1   Protein ACO-1
Caenorhabditis elegans CELE_F54H12.1   Protein ACO-2, isoform C
Dictyostelium discoideum DDB_G0278779   aconitase, mitochondrial
Dictyostelium discoideum DDB_G0279159   aconitate hydratase
Drosophila melanogaster Dmel_CG4900   Iron regulatory protein 1A
Drosophila melanogaster Dmel_CG4706   CG4706 gene product from transcript CG4706-RA
Drosophila melanogaster Dmel_CG6342   Iron regulatory protein 1B
Drosophila melanogaster Dmel_CG9244   Aconitase
Escherichia coli b0771   putative hydratase
Escherichia coli b1276   aconitate hydratase 1
Echinococcus granulosus EgrG_000158240   aconitate hydratase mitochondrial
Echinococcus multilocularis EmuJ_000158800   aconitate hydratase, mitochondrial
Homo sapiens ENSG00000100412   aconitase 2, mitochondrial
Homo sapiens ENSG00000122729   aconitase 1, soluble
Homo sapiens ENSG00000136381   iron-responsive element binding protein 2
Leishmania braziliensis LbrM.18.0570   aconitase, putative
Leishmania donovani LdBPK_180510.1   aconitase, putative
Leishmania infantum LinJ.18.0510   aconitase, putative
Leishmania major LmjF.18.0510   aconitase, putative
Leishmania mexicana LmxM.18.0510   aconitase, putative
Mycobacterium leprae ML2672c   PROBABLE ISOCITRATE DEHYDROGENASE [NADP] ICD2 (OXALOSUCCINATE DECARBOXYLASE) (IDH) (NADP+-SPECIFIC ICDH) (IDP)
Mycobacterium leprae ML1814   PROBABLE IRON-REGULATED ACONITATE HYDRATASE ACN (Citrate hydro-lyase) (Aconitase)
Mus musculus ENSMUSG00000028405   aconitase 1
Mus musculus ENSMUSG00000022477   aconitase 2, mitochondrial
Mus musculus ENSMUSG00000032293   iron responsive element binding protein 2
Mycobacterium tuberculosis Rv0066c   Probable isocitrate dehydrogenase [NADP] Icd2 (oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP)
Mycobacterium tuberculosis Rv1475c   Probable iron-regulated aconitate hydratase Acn (citrate hydro-lyase) (aconitase)
Mycobacterium ulcerans MUL_1484   aconitate hydratase
Mycobacterium ulcerans MUL_4939   isocitrate dehydrogenase [NADP] Icd2
Neospora caninum NCLIV_046260   Iron regulatory protein-like protein, related
Oryza sativa 4331547   Os03g0136900
Oryza sativa 4340833   Os06g0303400
Oryza sativa 9266421   Os09g0449600
Plasmodium berghei PBANKA_1355200   aconitate hydratase, putative
Plasmodium falciparum PF3D7_1342100   aconitate hydratase
Plasmodium knowlesi PKNH_1259300   aconitate hydratase, putative
Plasmodium vivax PVX_083005   aconitate hydratase I, putative
Plasmodium yoelii PY00319   aconitate hydratase 1
Saccharomyces cerevisiae YLR304C   aconitate hydratase ACO1
Saccharomyces cerevisiae YJL200C   aconitate hydratase ACO2
Schistosoma japonicum Sjp_0067360   ko:K01680 aconitate hydratase [EC4.2.1.3], putative
Schistosoma mansoni Smp_063090   aconitate hydratase
Schmidtea mediterranea mk4.000109.03   Aconitate hydratase, mitochondrial
Schmidtea mediterranea mk4.089615.01   Probable aconitate hydratase, mitochondrial
Trypanosoma brucei gambiense Tbg972.10.17200   aconitase, putative
Trypanosoma brucei Tb927.10.14000   aconitase
Trypanosoma congolense TcIL3000_10_11690   aconitase
Trypanosoma cruzi TcCLB.511277.290   aconitase, putative
Toxoplasma gondii TGME49_226730   aconitate hydratase ACN/IRP
Theileria parva TP01_1050   aconitate hydratase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0540   aconitate hydratase

Essentiality

TGME49_226730 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu66 Mycobacterium tuberculosis non-essential nmpdr
Tb927.10.14000 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.14000 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.14000 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.14000 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0771 Escherichia coli non-essential goodall
b1276 Escherichia coli non-essential goodall
CELE_F54H12.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_F54H12.1 Caenorhabditis elegans larval arrest wormbase
CELE_F54H12.1 Caenorhabditis elegans sterile wormbase
PBANKA_1355200 Plasmodium berghei Essential plasmo
TGME49_226730 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Aconitase (4.2.1.3 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

2 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_226730 (Toxoplasma gondii), aconitate hydratase ACN/IRP
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