Detailed view for TGME49_313050

Basic information

TDR Targets ID: 264970
Toxoplasma gondii, oxidoreductase, short chain dehydrogenase/reductase family protein
Source Database / ID:  ToxoDB 

pI: 7.9822 | Length (AA): 368 | MW (Da): 40367 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00106   short chain dehydrogenase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016491   oxidoreductase activity  
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 7 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 239 1vi2 (A) 48 285 12.00 0 0.04 0.51 -0.02
63 317 1iy8 (A) 10 259 21.00 0 1 1.07 -1.41
63 367 1zbq (A) 6 287 22.00 0 1 1.09 -0.53
62 317 3pgx (A) 7 269 23.00 0 1 0.945252 -0.66
63 362 3oml (A) 9 308 21.00 0 1 0.631817 1.07
63 128 3vpx (A) 170 235 21.00 0.96 0.61 0.339948 -0.16
65 264 2hq1 (A) 4 193 33.00 0.00000038 1 0.665178 -0.11

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Bradyzoite. Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 Oocyst. Gregory Fritz HM
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile ME49 merozoite. Hehl AB
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_131445)

Species Accession Gene Product
Arabidopsis thaliana AT3G03330   Rossmann-fold NAD(P)-binding domain-containing protein
Cryptosporidium hominis Chro.10039   dehydrogenase/reductase (SDR family) member 7; CGI-86 protein; retinal short-chain dehydrogenase/reductase 4; 2310016E22Rik
Cryptosporidium parvum cgd1_280   short chain dehydrogenase/reductase of the rossmann fold, signal peptide
Dictyostelium discoideum DDB_G0274201   short-chain dehydrogenase/reductase family protein
Drosophila melanogaster Dmel_CG31937   CG31937 gene product from transcript CG31937-RA
Homo sapiens ENSG00000100612   dehydrogenase/reductase (SDR family) member 7
Mus musculus 209183   predicted gene 4756
Mus musculus ENSMUSG00000021094   dehydrogenase/reductase (SDR family) member 7
Neospora caninum NCLIV_037010   oxidoreductase, short chain dehydrogenase/reductase domain-containing protein, putative
Neospora caninum NCLIV_056400   short-chain dehydrogenase/reductase family protein, putative
Oryza sativa 4350245   Os11g0265400
Toxoplasma gondii TGME49_313050   oxidoreductase, short chain dehydrogenase/reductase family protein
Toxoplasma gondii TGME49_269400   oxidoreductase, short chain dehydrogenase/reductase family protein

Essentiality

TGME49_313050 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
TGME49_269400 Toxoplasma gondii Probably non-essential sidik
TGME49_313050 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Cochliobolus lunatus 17-beta-hydroxysteroid-dehydrogenase 270 aa 22.9% 223 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0123 0.5 0.5
0.0053 1 1
0.008 0.5 0.5
0.0012 0.5 0.5
0.0058 1 1
0.0066 0.3586 0.5
0.007 0.2916 1
0.0005 0.5 0.5
0.0009 0.5 0.5
0.0009 0.5 0.5
0.0085 1 1
0.0132 0.3658 1
0.0021 0.3321 0.5
0.0009 1 0.5
0.0071 0.3141 0
0.0016 0.5 0.5
0.007 0.2916 1
0.0076 1 0.5
0.0004 0.5 0.5
0.0165 1 1
0.0082 1 0.5
0.0012 0.5 0.5
0.007 1 0.5
0.0056 1 0.5
0.0004 0.5 0.5
0.0106 0.2872 1
0.0402 1 1
0.0046 0.3082 0
0.0068 0.2585 0.5
0.0106 1 1
0.0131 0.5 0.5
0.0088 0.6544 0.5
0.0408 0.5144 1
0.0019 0.4322 1
0.016 0.577 1
0.0113 1 1
0.0097 1 1
0.0009 0.5 0.5
0.0045 1 1
0.0004 0.5 0.5
0.004 0.5 0.5
0.0009 0.5 0.5
0.0162 1 1
0.0171 0.4063 0.5
0.0093 0.5 0.5
0.0021 0.3321 0.5
0.0027 0.5 0.5
0.0057 0.284 0.6352
0.0032 1 1
0.0013 1 0.5
0.0171 0.4063 0.5
0.0073 0.3811 1
0.0009 0.5 0.5
0.0089 0.5 0.5
0.0196 0.8679 1
0.007 1 0.5
0.0068 1 1
0.0073 0.3141 1
0.0058 0.3931 0.5
0.0039 1 0.5
0.0053 1 1
0.0009 0.7374 0.5
0.0196 0.8679 1
0.011 0.3568 1
0.007 0.2916 1
0.0196 0.8679 1
0.0004 0.5 0.5
0.0157 0.5 0.5
0.0114 1 0.5
0.0004 0.5 0.5
0.0106 1 0.5
0.0222 0.5 0.5
0.0027 0.5 0.5
0.0087 0.5 0.5
0.0106 0.5 0.5
0.0073 0.4546 1
0.0073 0.2873 0.7466
0.016 0.577 1
0.0009 0.5 0.5
0.007 1 0.5
0.0108 0.5 0.5
0.0114 1 0.5
0.0088 1 0.5
0.0013 0.5 0.5
0.0009 0.5 0.5
0.0068 1 1
0.0084 0.4283 0.4047
0.0021 0.5 0.5
0.0408 0.5144 1
0.0119 0.2746 1
0.0089 0.5 0.5
0.0408 0.5144 1
0.0005 0.5 0.5
0.0035 1 0.5
0.0118 1 1
0.0073 0.3811 1
0.0102 0.7451 0.5
0.0064 0.3508 0.5
0.0097 0.4304 0.5
0.0067 0.3468 1
0.0026 0.5 0.5
0.0004 0.5 0.5
0.0009 0.5 0.5
0.0004 0.5 0.5
0.0056 0.875 0.5
0.0218 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_313050 (Toxoplasma gondii), oxidoreductase, short chain dehydrogenase/reductase family protein
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